SRR3553562_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240347
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	9042	3.762060687256342	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT	8578	3.5690064781336983	No Hit
AATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	7502	3.121320424219982	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	3618	1.505323553029578	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2512	1.0451555459398287	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1928	0.8021735241130532	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	1490	0.6199370077429717	No Hit
AACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	1209	0.5030227129941293	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT	959	0.39900643652718776	No Hit
AAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	707	0.2941580298485107	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	602	0.25047119373239524	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	601	0.25005512862652746	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	509	0.211777138886693	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.18140438615834606	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	380	0.15810474022975113	No Hit
GCATCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGT	340	0.1414621359950405	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	306	0.12731592239553646	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	285	0.11857855517231337	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.11608216453710676	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	272	0.1131697087960324	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG	269	0.11192151347842909	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGG	256	0.10651266710214814	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTA	250	0.10401627646694156	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	249	0.10360021136107378	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCTA	20	7.02214E-4	45.000004	30
TGATACA	20	7.02214E-4	45.000004	3
ATTAGAA	20	7.02214E-4	45.000004	13
TTTACGG	20	7.02214E-4	45.000004	2
CGGCCAC	20	7.02214E-4	45.000004	30
AGCCAAT	20	7.02214E-4	45.000004	35
CACGCTA	20	7.02214E-4	45.000004	18
ACACACG	20	7.02214E-4	45.000004	1
ACTTATG	20	7.02214E-4	45.000004	34
ACGGGCC	35	1.206572E-7	45.000004	5
TAGCATA	20	7.02214E-4	45.000004	30
GTAGCAT	20	7.02214E-4	45.000004	29
ACCTAAA	20	7.02214E-4	45.000004	10
TACGATT	20	7.02214E-4	45.000004	11
CGCTATA	20	7.02214E-4	45.000004	20
AAGCACG	20	7.02214E-4	45.000004	1
GGGCGAT	45	3.8198777E-10	45.000004	7
TCACTTG	20	7.02214E-4	45.000004	31
CATACCG	20	7.02214E-4	45.000004	1
CTACAGG	40	6.7757355E-9	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content