##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240347
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98539611478404	31.0	31.0	33.0	28.0	34.0
2	31.16920951790536	31.0	31.0	34.0	28.0	34.0
3	31.31370476852218	31.0	31.0	34.0	28.0	34.0
4	35.23345829155346	35.0	35.0	37.0	33.0	37.0
5	34.78434929497768	35.0	35.0	37.0	32.0	37.0
6	34.818566489284244	35.0	35.0	37.0	32.0	37.0
7	33.582075083109004	35.0	35.0	37.0	29.0	37.0
8	34.3544208997824	35.0	35.0	37.0	30.0	37.0
9	35.80952123388268	38.0	35.0	39.0	32.0	39.0
10	35.562440970763106	37.0	35.0	39.0	30.0	39.0
11	35.995518978809805	37.0	35.0	39.0	31.0	39.0
12	35.4975181716435	37.0	35.0	39.0	30.0	39.0
13	35.556216636779325	37.0	35.0	39.0	30.0	39.0
14	36.4298160576167	38.0	35.0	40.0	31.0	41.0
15	36.66679009931474	38.0	35.0	40.0	31.0	41.0
16	36.49736838820539	38.0	35.0	40.0	31.0	41.0
17	36.42067510724078	38.0	35.0	40.0	30.0	41.0
18	36.06172325845548	38.0	35.0	40.0	30.0	41.0
19	36.028454692590294	38.0	34.0	40.0	29.0	41.0
20	36.2611640669532	38.0	34.0	40.0	30.0	41.0
21	36.47152658447994	38.0	35.0	40.0	31.0	41.0
22	36.42621709445094	38.0	35.0	40.0	31.0	41.0
23	36.39487074937486	38.0	35.0	40.0	31.0	41.0
24	36.06171909780443	38.0	34.0	40.0	30.0	41.0
25	36.07256175446334	38.0	34.0	40.0	30.0	41.0
26	36.02383220926411	38.0	34.0	40.0	30.0	41.0
27	35.937394683520075	38.0	34.0	40.0	30.0	41.0
28	35.79378981222982	38.0	34.0	40.0	29.0	41.0
29	35.59456535758716	38.0	34.0	40.0	29.0	41.0
30	35.738798487187275	38.0	34.0	40.0	29.0	41.0
31	35.416697524828685	38.0	34.0	40.0	28.0	41.0
32	35.233649681502165	37.0	34.0	40.0	27.0	41.0
33	35.42445297840206	38.0	34.0	40.0	28.0	41.0
34	35.30636538005467	38.0	34.0	40.0	28.0	41.0
35	35.233957569680506	37.0	34.0	40.0	27.0	41.0
36	35.06855088684277	37.0	34.0	40.0	27.0	41.0
37	35.083458499586015	37.0	34.0	40.0	27.0	41.0
38	34.931482398365695	37.0	34.0	40.0	27.0	41.0
39	34.749761802726894	37.0	33.0	40.0	26.0	41.0
40	34.47775091846372	37.0	33.0	40.0	25.0	41.0
41	34.660827886347654	37.0	33.0	40.0	25.0	41.0
42	34.52784931786126	37.0	33.0	40.0	24.0	41.0
43	34.70046224833262	37.0	33.0	40.0	26.0	41.0
44	34.66195542278456	37.0	33.0	40.0	26.0	41.0
45	34.39795379180934	37.0	33.0	40.0	24.0	40.0
46	34.25991587163559	37.0	33.0	39.0	24.0	40.0
47	34.10995352552767	36.0	33.0	39.0	23.0	40.0
48	33.98838762289523	36.0	33.0	39.0	23.0	40.0
49	33.79894485889152	36.0	33.0	39.0	23.0	40.0
50	33.658185872925394	36.0	33.0	39.0	23.0	40.0
51	31.22975115146018	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	4.0
12	6.0
13	5.0
14	6.0
15	21.0
16	43.0
17	98.0
18	188.0
19	363.0
20	623.0
21	950.0
22	1350.0
23	1652.0
24	1917.0
25	2328.0
26	2694.0
27	3406.0
28	4144.0
29	5509.0
30	7228.0
31	9604.0
32	12443.0
33	16940.0
34	24773.0
35	26500.0
36	27866.0
37	34215.0
38	36866.0
39	18600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.319762676463615	43.60819981110644	16.582274794359822	21.489762718070125
2	19.48599316821096	43.32818799485744	17.264621567982957	19.921197268948646
3	17.85460188810345	28.10769429200281	31.484062626119737	22.553641193774002
4	27.86596046549364	28.59407440076223	17.358236216803206	26.181728916940923
5	13.981451817580414	42.48815254611041	18.38009211681444	25.15030351949473
6	20.504104482269387	32.91574265541072	28.07191269289818	18.50824016942171
7	64.33905977607375	19.928270375748397	9.377275356047713	6.355394492130129
8	68.87874614619696	6.6861662512950035	6.647056131343433	17.788031471164608
9	68.56212060063159	9.84742892567829	12.884288133407114	8.706162340283008
10	36.5030559982026	27.920464994362316	15.672340407827019	19.904138599608068
11	29.183638655776857	24.710106637486636	24.985541737571097	21.120712969165414
12	26.410564725168197	22.242008429479046	36.45936916208649	14.888057683266279
13	25.39120521579217	31.772395744486097	21.508069582728307	21.32832945699343
14	15.718523634578338	32.51632015377766	35.897681269165	15.867474942479
15	13.394383953200997	31.674204379501305	27.929202361585542	27.00220930571216
16	15.084440413235864	26.824549505506624	35.11755919566294	22.97345088559458
17	13.908640424053557	26.394338186039352	29.943373539091393	29.753647850815696
18	16.976704514722464	28.97851855858405	27.34255056231199	26.7022263643815
19	18.965495720770388	31.828980598884115	30.914469496186765	18.291054184158735
20	20.79368579595335	34.253808035881455	23.62334458096003	21.329161587205167
21	17.563356313996014	27.987035411301157	27.595102081573724	26.8545061931291
22	17.699409603614775	32.59870104473948	25.661231469500343	24.040657882145396
23	22.387631216532764	27.618817792608187	20.821146092940623	29.172404897918426
24	14.722047706025037	39.20248640507267	23.872983644480687	22.202482244421606
25	17.612035931382543	25.190245769658038	28.330705188747935	28.867013110211488
26	21.893345870761856	33.325566784690466	19.803450843988067	24.97763650055961
27	14.732449333671733	27.142007181283727	30.481761786084288	27.64378169896025
28	19.343698902004185	27.9891157368305	30.341547845406847	22.325637515758466
29	16.423753989024203	38.159411184662176	25.352927226052337	20.063907600261288
30	20.43254128406013	25.81184703782448	23.424881525461107	30.33073015265429
31	25.71781632389836	36.220963856424255	19.110702442718235	18.950517376959148
32	19.46976662908212	29.887204749799253	20.86524899416261	29.777779626956026
33	22.454617698577472	26.069391338356624	25.681618659687867	25.794372303378033
34	20.49786350568137	31.556874019646596	21.476448634682356	26.46881383998968
35	17.68318306448593	24.301114638418618	31.75325674961618	26.262445547479267
36	22.67263581405218	31.906784773681384	26.2607812870558	19.159798125210635
37	18.509488364739315	31.788206218509075	20.437118000224675	29.26518741652694
38	21.428185082401694	30.613238359538503	24.607338556337297	23.35123800172251
39	23.1964617823397	28.35816548573521	25.040462331545637	23.40491040037945
40	20.703815733085914	26.879470099481168	25.259728642337954	27.156985525094967
41	23.08121174801433	20.963440359147402	33.278967492833274	22.67638040000499
42	24.503322279870353	22.874843455503918	26.309044839336458	26.312789425289267
43	21.11988083895368	23.926239978031763	27.645445959383725	27.308433223630836
44	19.746865989590052	21.5559170699031	26.66686083038274	32.03035611012412
45	27.237286090527448	28.746770294615704	23.203118824033584	20.812824790823267
46	18.45456777076477	23.006736094064	37.67802385717317	20.86067227799806
47	23.99530678560581	26.661868049112325	21.789329594294916	27.553495570986946
48	19.042051700250052	21.493091238917067	35.11797526076881	24.346881800064075
49	23.194381456810362	19.746865989590052	30.916133756610236	26.142618796989353
50	22.135911827482765	21.15691063337591	25.29342991591324	31.413747623228083
51	18.524882773656422	21.012952106745665	35.06471892721773	25.397446192380187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2924.0
1	2291.0
2	1658.0
3	1108.5
4	559.0
5	523.5
6	488.0
7	513.5
8	539.0
9	574.0
10	609.0
11	621.5
12	634.0
13	646.5
14	659.0
15	683.5
16	708.0
17	728.0
18	748.0
19	702.0
20	656.0
21	669.5
22	683.0
23	695.5
24	708.0
25	794.5
26	1026.0
27	1171.0
28	1374.5
29	1578.0
30	2082.0
31	2586.0
32	2743.0
33	2900.0
34	3237.0
35	3574.0
36	3917.0
37	4260.0
38	4823.5
39	5387.0
40	7448.5
41	9510.0
42	23117.0
43	36724.0
44	34898.5
45	33073.0
46	29299.0
47	25525.0
48	22230.5
49	18936.0
50	17579.5
51	16223.0
52	14863.5
53	13504.0
54	12171.5
55	10839.0
56	9958.5
57	9078.0
58	8493.5
59	7909.0
60	7356.5
61	6804.0
62	5907.5
63	5011.0
64	4319.0
65	3627.0
66	3183.5
67	2740.0
68	2437.0
69	2134.0
70	1869.5
71	1605.0
72	1374.5
73	1144.0
74	1022.5
75	727.5
76	554.0
77	396.5
78	239.0
79	177.0
80	115.0
81	85.0
82	55.0
83	69.0
84	83.0
85	66.5
86	50.0
87	38.0
88	26.0
89	22.0
90	18.0
91	10.0
92	2.0
93	2.5
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.290176286785353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62635463067616	24.915226734679443
2	8.532677348580545	5.339779568706912
3	2.679343128781331	2.5151135649706466
4	1.3376770161558407	1.6742459860118912
5	0.8869091150854331	1.3875771280690004
6	0.5983644704474437	1.1233757858429687
7	0.4454491057775413	0.9756726732599117
8	0.4281630210757264	1.0717837127153658
9	0.32843560933448573	0.9249127303440442
>10	4.769629678877734	34.25588836141079
>50	0.2752476564058241	5.422992589880464
>100	0.07446313410012632	4.059963303057663
>500	0.006648494116082707	1.4054679276213142
>1k	0.006648494116082707	4.47561234381956
>5k	0.003989096469649624	10.452387589610023
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	9042	3.762060687256342	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT	8578	3.5690064781336983	No Hit
AATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	7502	3.121320424219982	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	3618	1.505323553029578	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2512	1.0451555459398287	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1928	0.8021735241130532	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	1490	0.6199370077429717	No Hit
AACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	1209	0.5030227129941293	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT	959	0.39900643652718776	No Hit
AAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	707	0.2941580298485107	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	602	0.25047119373239524	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	601	0.25005512862652746	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	509	0.211777138886693	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.18140438615834606	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	380	0.15810474022975113	No Hit
GCATCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGT	340	0.1414621359950405	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	306	0.12731592239553646	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	285	0.11857855517231337	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.11608216453710676	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	272	0.1131697087960324	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG	269	0.11192151347842909	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGG	256	0.10651266710214814	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTA	250	0.10401627646694156	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	249	0.10360021136107378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8552343070643695	0.0
2	0.0	0.0	0.0	2.147728076489409	0.0
3	0.0	0.0	0.0	3.1042617548794036	0.0
4	0.0	0.0	0.0	8.180256046466152	0.0
5	0.0	0.0	0.0	8.729461986211602	0.0
6	0.0	0.0	0.0	10.8979933179944	0.0
7	0.0	0.0	0.0	12.535625574689927	0.0
8	0.0	0.0	0.0	13.146825215209676	0.0
9	0.0	0.0	0.0	18.151672373693035	0.0
10	0.0	0.0	0.0	19.523022962633192	0.0
11	0.0	0.0	0.0	25.08206884213242	0.0
12	0.0	0.0	0.0	25.937498699796546	0.0
13	0.0	0.0	0.0	26.475886946789434	0.0
14	0.0	0.0	0.0	27.273067689632075	0.0
15	0.0	0.0	0.0	27.72241800396926	0.0
16	0.0	0.0	0.0	28.35941368105281	0.0
17	0.0	0.0	0.0	28.988088056019006	0.0
18	0.0	0.0	0.0	30.217560443858254	0.0
19	0.0	0.0	0.0	30.554989244717014	0.0
20	0.0	0.0	0.0	31.17034953629544	0.0
21	0.0	0.0	0.0	31.539399285200147	0.0
22	0.0	0.0	0.0	31.91718640132808	0.0
23	0.0	0.0	0.0	32.351558371854026	0.0
24	0.0	0.0	0.0	32.63448264384411	0.0
25	0.0	0.0	0.0	32.85874173590683	0.0
26	0.0	0.0	0.0	33.10172375773361	0.0
27	0.0	0.0	0.0	33.395049657370386	0.0
28	0.0	0.0	0.0	33.66923656213724	0.0
29	0.0	0.0	0.0	33.888918938035424	0.0
30	0.0	0.0	0.0	34.27045064011617	0.0
31	0.0	0.0	0.0	34.51634511768402	0.0
32	0.0	0.0	0.0	34.74684518633476	0.0
33	0.0	0.0	0.0	34.99898064049062	0.0
34	0.0	0.0	0.0	35.2161666257536	0.0
35	0.0	0.0	0.0	35.4807840330855	0.0
36	0.0	0.0	0.0	35.71627688300665	0.0
37	0.0	0.0	0.0	35.9467769516574	0.0
38	0.0	0.0	0.0	36.1880947130607	0.0
39	0.0	0.0	0.0	36.438565906793094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCTA	20	7.02214E-4	45.000004	30
TGATACA	20	7.02214E-4	45.000004	3
ATTAGAA	20	7.02214E-4	45.000004	13
TTTACGG	20	7.02214E-4	45.000004	2
CGGCCAC	20	7.02214E-4	45.000004	30
AGCCAAT	20	7.02214E-4	45.000004	35
CACGCTA	20	7.02214E-4	45.000004	18
ACACACG	20	7.02214E-4	45.000004	1
ACTTATG	20	7.02214E-4	45.000004	34
ACGGGCC	35	1.206572E-7	45.000004	5
TAGCATA	20	7.02214E-4	45.000004	30
GTAGCAT	20	7.02214E-4	45.000004	29
ACCTAAA	20	7.02214E-4	45.000004	10
TACGATT	20	7.02214E-4	45.000004	11
CGCTATA	20	7.02214E-4	45.000004	20
AAGCACG	20	7.02214E-4	45.000004	1
GGGCGAT	45	3.8198777E-10	45.000004	7
TCACTTG	20	7.02214E-4	45.000004	31
CATACCG	20	7.02214E-4	45.000004	1
CTACAGG	40	6.7757355E-9	45.000004	2
>>END_MODULE