SRR3553561_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553561_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275055
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT	9681	3.519659704422752	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	9612	3.4945738125102253	No Hit
AATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	7964	2.8954209158168367	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	3706	1.347366890258312	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3057	1.1114140808201995	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1933	0.7027685372016506	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	1609	0.5849739143080475	No Hit
AACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	1300	0.47263274617803713	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT	1068	0.38828597916780283	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	750	0.2726727381796368	No Hit
AAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	736	0.26758284706695024	No Hit
AATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	617	0.22431877260911454	No Hit
AATCTGACTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	564	0.20504989911108687	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	532	0.19341586228208904	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	502	0.18250895275490356	No Hit
AATCTGTCACTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	474	0.17232917052953045	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	464	0.16869353402046863	No Hit
AATGATACATGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT	446	0.16214938830415734	No Hit
GCATCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGT	390	0.14178982385341113	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	385	0.13997200559888023	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	343	0.12470233226082056	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG	323	0.11743105924269691	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	298	0.10834196797004235	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	283	0.10288851320644962	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	281	0.10216138590463725	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	20	7.0239196E-4	45.000004	40
AGATCTA	20	7.0239196E-4	45.000004	42
GGTACAA	40	6.7830115E-9	45.000004	8
CTACTCG	20	7.0239196E-4	45.000004	1
ATCTAAC	20	7.0239196E-4	45.000004	44
CTATCTC	20	7.0239196E-4	45.000004	7
ACGCATG	20	7.0239196E-4	45.000004	1
CCCTATT	20	7.0239196E-4	45.000004	19
TAAACGG	20	7.0239196E-4	45.000004	2
CGATTAA	20	7.0239196E-4	45.000004	24
AAGCGCG	20	7.0239196E-4	45.000004	1
TACGGAA	20	7.0239196E-4	45.000004	4
ATACTAG	20	7.0239196E-4	45.000004	1
ATACTAC	20	7.0239196E-4	45.000004	20
GCATTAC	20	7.0239196E-4	45.000004	12
TATCTGC	20	7.0239196E-4	45.000004	45
CTACGTC	20	7.0239196E-4	45.000004	23
TTAAGGG	40	6.7830115E-9	45.000004	3
ATCCCGC	20	7.0239196E-4	45.000004	31
TGTTCGG	20	7.0239196E-4	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content