FastQCFastQC Report
Sat 18 Jun 2016
SRR3553558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209075
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27771.3282314958746861No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT18200.8705010163816812No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT16550.7915819681932321No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC16420.7853641037905057No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT16140.7719717804615569No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7080.33863446131770897No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.30037068037785486No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5440.260193710391008No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4600.2200167404041612No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4000.1913189046992706No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT3640.17410020327633624No Hit
AACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG3520.16836063613535812No Hit
TACTAGGGATCTTTGCTTATATTTGACATTGAGCAGTCAGGGGATTTAGGC2990.14301088126270478No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.12961855793375582No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA2600.1243572880545259No Hit
GATAGAGGGAAAGGATAAAAAGGAAGGAAGGAAGGATGAAGAAAGGAAGAA2530.12100920722228865No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT2480.11861772091354776No Hit
AGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAATCCTTCGACTGGCGCA2310.11048666746382876No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT2190.10474710032285066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGT302.1569576E-645.0000048
CTGCGCG302.1569576E-645.0000041
TATCTCA302.1569576E-645.00000424
AGTTGAC302.1569576E-645.00000415
GACGGCC302.1569576E-645.00000419
AGCTACG302.1569576E-645.0000041
TCCTTCG302.1569576E-645.00000436
TTCGACT302.1569576E-645.00000439
GTTATTG302.1569576E-645.00000433
ACGCGAG207.020037E-445.01
TCCGGCA207.020037E-445.022
CCTTCCG207.020037E-445.013
TCGCCGG207.020037E-445.032
CTTCGAG253.8798258E-545.01
CGGGTAT253.8798258E-545.06
CAGCGGT207.020037E-445.026
GGTACGA351.2055534E-745.09
TGCCCGG253.8798258E-545.02
CGAACAG207.020037E-445.01
CTCCGGC207.020037E-445.021
Produced by FastQC (version 0.11.5)