##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209075
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.891498266172427	31.0	31.0	33.0	28.0	34.0
2	31.045615209852922	31.0	31.0	34.0	28.0	34.0
3	31.317728088006696	31.0	31.0	34.0	28.0	34.0
4	35.320210450795166	37.0	35.0	37.0	33.0	37.0
5	34.89574554585675	35.0	35.0	37.0	32.0	37.0
6	34.936520387420785	35.0	35.0	37.0	32.0	37.0
7	33.83537008250628	35.0	35.0	37.0	30.0	37.0
8	34.628348678703816	37.0	35.0	37.0	32.0	37.0
9	35.949931842640204	39.0	35.0	39.0	32.0	39.0
10	35.568427597752006	37.0	35.0	39.0	30.0	39.0
11	35.945780222408224	37.0	35.0	39.0	31.0	39.0
12	35.77052732273108	37.0	35.0	39.0	31.0	39.0
13	35.478962094942005	37.0	35.0	39.0	30.0	39.0
14	36.46744947985173	38.0	35.0	40.0	31.0	41.0
15	36.62469448762406	38.0	35.0	40.0	31.0	41.0
16	36.63270118378572	38.0	35.0	40.0	31.0	41.0
17	36.56329546813345	38.0	35.0	40.0	31.0	41.0
18	36.346598110725814	38.0	35.0	40.0	31.0	41.0
19	36.34010761688389	38.0	35.0	40.0	30.0	41.0
20	36.342006457013035	38.0	35.0	40.0	31.0	41.0
21	36.432285065168	38.0	35.0	40.0	31.0	41.0
22	36.37675953605166	38.0	35.0	40.0	31.0	41.0
23	36.33411455219419	38.0	35.0	40.0	31.0	41.0
24	36.001807963649405	38.0	34.0	40.0	30.0	41.0
25	36.086390051416956	38.0	34.0	40.0	30.0	41.0
26	35.976491689585075	38.0	34.0	40.0	30.0	41.0
27	35.84712662920005	38.0	34.0	40.0	30.0	41.0
28	35.789769221571206	38.0	34.0	40.0	29.0	41.0
29	35.60358722946311	38.0	34.0	40.0	29.0	41.0
30	35.59647973215353	38.0	34.0	40.0	29.0	41.0
31	35.41459763242855	38.0	34.0	40.0	28.0	41.0
32	35.206834867870384	38.0	34.0	40.0	27.0	41.0
33	35.24294631113237	38.0	34.0	40.0	27.0	41.0
34	35.1719000358723	38.0	34.0	40.0	27.0	41.0
35	35.17987325122564	38.0	34.0	40.0	27.0	41.0
36	35.04581131173024	37.0	34.0	40.0	27.0	41.0
37	34.97713739088844	37.0	34.0	40.0	27.0	41.0
38	34.88611742197776	37.0	33.0	40.0	26.0	41.0
39	34.716996293196225	37.0	33.0	40.0	25.0	41.0
40	34.5380844194667	37.0	33.0	40.0	24.0	41.0
41	34.619327992347245	37.0	33.0	40.0	25.0	41.0
42	34.59643668539998	37.0	33.0	40.0	25.0	41.0
43	34.599325600860936	37.0	33.0	40.0	25.0	41.0
44	34.53147435130934	37.0	33.0	40.0	25.0	41.0
45	34.33267009446371	37.0	33.0	40.0	24.0	41.0
46	34.18850651680019	37.0	33.0	40.0	24.0	41.0
47	33.996398421619034	36.0	33.0	40.0	23.0	40.0
48	33.93314839172546	36.0	33.0	39.0	23.0	40.0
49	33.80169795527921	36.0	33.0	39.0	23.0	40.0
50	33.66945832835107	36.0	33.0	39.0	23.0	40.0
51	31.508769580294153	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	0.0
11	3.0
12	3.0
13	5.0
14	15.0
15	23.0
16	34.0
17	95.0
18	219.0
19	301.0
20	589.0
21	869.0
22	1212.0
23	1517.0
24	1852.0
25	2128.0
26	2452.0
27	3015.0
28	3769.0
29	4781.0
30	6036.0
31	7742.0
32	10300.0
33	14062.0
34	21444.0
35	23328.0
36	23262.0
37	29696.0
38	34036.0
39	16283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.16262106899438	37.04986248953725	17.607557096735622	25.17995934473275
2	20.6949659213201	36.86380485471721	19.657539160588307	22.783690063374387
3	19.489656821714696	34.59093626688987	21.207700585914147	24.711706325481288
4	19.67762764558173	34.32022001674041	18.58184861891666	27.420303718761208
5	16.09709434413488	38.367093148391724	18.678942963051536	26.85686954442186
6	19.54035633146	38.05093865837618	21.284228147793854	21.124476862369963
7	75.6326677029774	12.772928374985055	6.607676671051058	4.986727250986489
8	79.36099485830444	7.979433217744829	5.254573717565466	7.404998206385269
9	72.9460719837379	12.741839052971422	9.545856749970106	4.766232213320579
10	35.9741719478656	38.08824584479254	11.977041731436087	13.960540475905775
11	27.918689465502812	26.85686954442186	28.686356570608634	16.5380844194667
12	27.39256247757982	26.22456056439077	29.96149707042927	16.421379887600143
13	22.162860217625255	35.55614014109769	24.629439196460602	17.651560444816454
14	15.641755350950614	37.66112639005142	30.91952648571087	15.777591773287098
15	14.824345330622982	32.419944995814895	32.871935908166925	19.883773765395194
16	17.43250029893579	29.727609709434414	32.55195504005739	20.287934951572403
17	16.58208776754753	29.543465263661368	27.69101996891068	26.183426999880428
18	19.549443979433217	29.56833672127227	26.48092789668779	24.40129140260672
19	19.70106421140739	33.76300370680378	26.709075690541674	19.826856391247162
20	22.64928853282315	32.923592012435726	24.78966877914624	19.637450675594884
21	19.319861293794094	32.117661126390054	25.56307545139304	22.999402128422812
22	19.56761927537965	30.878871218462272	25.820877675475305	23.732631830682767
23	20.239148630874087	32.02630634939615	22.347482960660052	25.38706205906971
24	16.316154490015546	33.88496950854956	27.59870859739328	22.20016740404161
25	18.46897046514409	30.37618079636494	26.52349635298338	24.631352385507594
26	21.965801745785004	35.24333373191438	21.231615449001552	21.559249073299057
27	16.54525887839292	33.22874566543106	24.548606959225157	25.677388496950854
28	18.417314360875285	30.62824345330623	28.477818964486428	22.476623221332055
29	20.53856271672845	31.708716967595365	26.214516321894056	21.538203993782133
30	20.628482601937105	30.572760970943442	23.269161784048787	25.52959464307067
31	24.229582685639127	32.662920004782976	22.46083941169437	20.646657897883536
32	22.427836900633743	32.298457491330865	21.957670692335288	23.316034915700108
33	20.77914623938778	32.575869903144806	22.804735142891307	23.84024871457611
34	18.275738371397825	33.938538801865356	23.167762764558173	24.617960062178646
35	18.212124835585318	31.096496472557693	26.236996293196224	24.45438239866077
36	23.237594164773405	30.17529594643071	27.09219179720196	19.494918091593927
37	18.538801865359318	32.14253258400095	25.23065885447806	24.088006696161663
38	19.60636135358125	31.42939136673443	27.877077603730722	21.087169675953604
39	18.969269400932678	32.747100322850656	26.219777591773287	22.06385268444338
40	21.598947746024155	32.059308860456774	23.75367691019969	22.588066483319384
41	19.20746143728327	28.078440750926703	28.805930886045676	23.90816692574435
42	22.959703455697717	27.639363864641876	25.801267487743633	23.599665191916777
43	21.22204950376659	26.70429271792419	27.261987325122565	24.811670453186654
44	20.98624895372474	27.636972378333134	25.370799952170277	26.005978715771853
45	23.31316513212962	30.472796843238072	24.641874925266052	21.572163099366257
46	19.902427358603372	29.475068755231376	28.910677986368526	21.711825899796725
47	21.079995217027385	29.92323328948942	23.915341384670572	25.081430108812626
48	20.826975965562596	26.446012196580178	31.160588305631947	21.56642353222528
49	21.588903503527444	24.366375702499106	29.302403443740282	24.74231735023317
50	19.899079277771133	27.647494918091592	24.176013392323327	28.27741241181394
51	19.122802821953844	27.030969747698197	29.612818366614853	24.23340906373311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3172.0
1	2498.0
2	1824.0
3	1203.0
4	582.0
5	526.5
6	471.0
7	499.5
8	528.0
9	574.5
10	621.0
11	634.5
12	648.0
13	698.0
14	748.0
15	714.0
16	680.0
17	718.5
18	757.0
19	756.5
20	756.0
21	735.0
22	714.0
23	785.5
24	857.0
25	931.0
26	1128.5
27	1252.0
28	1551.0
29	1850.0
30	2539.0
31	3228.0
32	3229.5
33	3231.0
34	3830.5
35	4430.0
36	4875.5
37	5321.0
38	6257.0
39	7193.0
40	9079.5
41	10966.0
42	16344.0
43	21722.0
44	23119.5
45	24517.0
46	24410.0
47	24303.0
48	21947.5
49	19592.0
50	18041.5
51	16491.0
52	15309.5
53	14128.0
54	12231.0
55	10334.0
56	8848.5
57	7363.0
58	6818.0
59	6273.0
60	5300.5
61	4328.0
62	3650.5
63	2973.0
64	2429.5
65	1886.0
66	1672.5
67	1459.0
68	1201.5
69	944.0
70	757.5
71	571.0
72	523.0
73	475.0
74	426.0
75	372.5
76	368.0
77	212.5
78	57.0
79	50.0
80	43.0
81	28.5
82	14.0
83	10.0
84	6.0
85	6.5
86	7.0
87	6.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	209075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.92729881621428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12170871367307	31.99043405476504
2	8.656172883873596	6.912352026784646
3	2.8294880088166945	3.3892143967475787
4	1.4279211289201945	2.2805213440153054
5	0.8780756606531062	1.752959464307067
6	0.6516687031313639	1.5611622623460482
7	0.4971369702196986	1.3894535453784527
8	0.40729293945710243	1.30096855195504
9	0.3509906801792089	1.2612698792299415
>10	3.8704808452526414	33.29570728207581
>50	0.25875080859627686	6.22934353700825
>100	0.040729293945710246	3.1897644385985893
>500	0.003593761230503845	0.8991988520865718
>1k	0.005989602050839742	4.547650364701663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2777	1.3282314958746861	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1820	0.8705010163816812	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT	1655	0.7915819681932321	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	1642	0.7853641037905057	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	1614	0.7719717804615569	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	708	0.33863446131770897	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.30037068037785486	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	544	0.260193710391008	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT	460	0.2200167404041612	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT	400	0.1913189046992706	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	364	0.17410020327633624	No Hit
AACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	352	0.16836063613535812	No Hit
TACTAGGGATCTTTGCTTATATTTGACATTGAGCAGTCAGGGGATTTAGGC	299	0.14301088126270478	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	271	0.12961855793375582	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	260	0.1243572880545259	No Hit
GATAGAGGGAAAGGATAAAAAGGAAGGAAGGAAGGATGAAGAAAGGAAGAA	253	0.12100920722228865	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	248	0.11861772091354776	No Hit
AGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAATCCTTCGACTGGCGCA	231	0.11048666746382876	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	219	0.10474710032285066	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.782972617481765E-4	0.0	0.0	1.124476862369963	0.0
2	4.782972617481765E-4	0.0	0.0	1.4176730838215952	0.0
3	4.782972617481765E-4	0.0	0.0	1.9925863924429033	0.0
4	4.782972617481765E-4	0.0	0.0	4.800669616166448	0.0
5	4.782972617481765E-4	0.0	0.0	5.242616286021763	0.0
6	4.782972617481765E-4	0.0	0.0	7.0237952887719715	0.0
7	4.782972617481765E-4	0.0	0.0	8.436207102714336	0.0
8	4.782972617481765E-4	0.0	0.0	9.150304914504364	0.0
9	4.782972617481765E-4	0.0	0.0	11.081190960181752	0.0
10	4.782972617481765E-4	0.0	0.0	12.709793136434294	0.0
11	4.782972617481765E-4	0.0	0.0	15.300729403324166	0.0
12	4.782972617481765E-4	0.0	0.0	16.282673681693172	0.0
13	4.782972617481765E-4	0.0	0.0	16.748535214635897	0.0
14	4.782972617481765E-4	0.0	0.0	17.20674399139065	0.0
15	4.782972617481765E-4	0.0	0.0	17.58412053090996	0.0
16	4.782972617481765E-4	0.0	0.0	18.283391127585794	0.0
17	4.782972617481765E-4	0.0	0.0	19.16632787277293	0.0
18	4.782972617481765E-4	0.0	0.0	20.24680138706206	0.0
19	4.782972617481765E-4	0.0	0.0	20.755231376300372	0.0
20	4.782972617481765E-4	0.0	0.0	21.238789907927778	0.0
21	4.782972617481765E-4	0.0	0.0	21.799354298696638	0.0
22	4.782972617481765E-4	0.0	0.0	22.41300968551955	0.0
23	4.782972617481765E-4	0.0	0.0	23.010402965443024	0.0
24	4.782972617481765E-4	0.0	0.0	23.44661006815736	0.0
25	4.782972617481765E-4	0.0	0.0	23.82398660767667	0.0
26	4.782972617481765E-4	0.0	0.0	24.155924907329904	0.0
27	4.782972617481765E-4	0.0	0.0	24.53999760851369	0.0
28	4.782972617481765E-4	0.0	0.0	24.93315795767069	0.0
29	4.782972617481765E-4	0.0	0.0	25.349276575391606	0.0
30	4.782972617481765E-4	0.0	0.0	25.950974530670813	0.0
31	4.782972617481765E-4	0.0	0.0	26.362310175774244	0.0
32	4.782972617481765E-4	0.0	0.0	26.723902905655866	0.0
33	4.782972617481765E-4	0.0	0.0	27.123281119215594	0.0
34	4.782972617481765E-4	0.0	0.0	27.539878034198253	0.0
35	4.782972617481765E-4	0.0	0.0	27.995695324644267	0.0
36	4.782972617481765E-4	0.0	0.0	28.404639483438956	0.0
37	4.782972617481765E-4	0.0	0.0	28.79445175176372	0.0
38	4.782972617481765E-4	0.0	0.0	29.199569532464427	0.0
39	4.782972617481765E-4	0.0	0.0	29.59512136793017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTAGT	30	2.1569576E-6	45.000004	8
CTGCGCG	30	2.1569576E-6	45.000004	1
TATCTCA	30	2.1569576E-6	45.000004	24
AGTTGAC	30	2.1569576E-6	45.000004	15
GACGGCC	30	2.1569576E-6	45.000004	19
AGCTACG	30	2.1569576E-6	45.000004	1
TCCTTCG	30	2.1569576E-6	45.000004	36
TTCGACT	30	2.1569576E-6	45.000004	39
GTTATTG	30	2.1569576E-6	45.000004	33
ACGCGAG	20	7.020037E-4	45.0	1
TCCGGCA	20	7.020037E-4	45.0	22
CCTTCCG	20	7.020037E-4	45.0	13
TCGCCGG	20	7.020037E-4	45.0	32
CTTCGAG	25	3.8798258E-5	45.0	1
CGGGTAT	25	3.8798258E-5	45.0	6
CAGCGGT	20	7.020037E-4	45.0	26
GGTACGA	35	1.2055534E-7	45.0	9
TGCCCGG	25	3.8798258E-5	45.0	2
CGAACAG	20	7.020037E-4	45.0	1
CTCCGGC	20	7.020037E-4	45.0	21
>>END_MODULE