FastQCFastQC Report
Sat 18 Jun 2016
SRR3553557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245043
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33001.3467024154944234No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT19980.8153670988357146No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT18180.7419106034451096No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT18140.740278236880874No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC16950.691715331594863No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.31871957166701353No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7130.29096934007500724No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.2815832323306522No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4640.18935452145133713No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4250.17343894745003938No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.15670719016662382No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT3480.14201589108850282No Hit
AACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG3310.13507833319050125No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA3230.13181360006202994No Hit
TACTAGGGATCTTTGCTTATATTTGACATTGAGCAGTCAGGGGATTTAGGC3040.12405985888191051No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT2680.10936855980378953No Hit
GATAGAGGGAAAGGATAAAAAGGAAGGAAGGAAGGATGAAGAAAGGAAGAA2670.10896046816273061No Hit
AGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAATCCTTCGACTGGCGCA2630.10732810159849496No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC2500.10202291026472905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG950.045.0000043
ATCCGGG453.8198777E-1045.0000043
AGTGCGC453.8198777E-1045.00000413
CAGGACG453.8198777E-1045.0000041
GCTACGG453.8198777E-1045.0000042
CTTAATG406.7775545E-945.045
TCGCCGG207.0224126E-445.032
CGGGTCA406.7775545E-945.06
GAGTGCG207.0224126E-445.040
CGGAACG207.0224126E-445.01
TTGGCGG207.0224126E-445.02
AGTCCGG406.7775545E-945.02
AAATCGC207.0224126E-445.045
TGCCCCG207.0224126E-445.01
GCGACGG502.1827873E-1145.02
AATCAGC207.0224126E-445.023
ACGCATG253.8817932E-545.01
CTATCGG351.2066994E-745.02
CGTGCGG207.0224126E-445.02
CCAGGTG207.0224126E-445.020
Produced by FastQC (version 0.11.5)