##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245043
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902033520647397	31.0	31.0	33.0	28.0	34.0
2	31.074505290908128	31.0	31.0	34.0	28.0	34.0
3	31.34702072697445	31.0	31.0	34.0	28.0	34.0
4	35.341593108148366	37.0	35.0	37.0	33.0	37.0
5	34.961541443746604	35.0	35.0	37.0	32.0	37.0
6	34.98002391417017	35.0	35.0	37.0	32.0	37.0
7	32.56605167256359	35.0	35.0	37.0	26.0	37.0
8	34.02523230616667	36.0	35.0	37.0	28.0	37.0
9	34.250082638557316	38.0	34.0	39.0	28.0	39.0
10	34.74854617352873	37.0	34.0	39.0	27.0	39.0
11	35.63740649600274	37.0	35.0	39.0	30.0	39.0
12	35.710087617275335	37.0	35.0	39.0	31.0	39.0
13	35.55571471129557	37.0	35.0	39.0	30.0	39.0
14	36.57356055875908	38.0	35.0	40.0	31.0	41.0
15	36.72968825879539	38.0	35.0	40.0	31.0	41.0
16	36.76360067416739	38.0	35.0	40.0	31.0	41.0
17	36.68643462575956	38.0	35.0	40.0	31.0	41.0
18	36.52865823549336	38.0	35.0	40.0	31.0	41.0
19	36.475671616818275	38.0	35.0	40.0	31.0	41.0
20	36.44674608130002	38.0	35.0	40.0	31.0	41.0
21	36.5350693551744	38.0	35.0	40.0	31.0	41.0
22	36.50775578163832	38.0	35.0	40.0	31.0	41.0
23	36.44710520194415	38.0	35.0	40.0	31.0	41.0
24	36.11877915304661	38.0	34.0	40.0	30.0	41.0
25	36.20415192435613	38.0	35.0	40.0	30.0	41.0
26	36.10349204017254	38.0	35.0	40.0	30.0	41.0
27	35.986141207869636	38.0	34.0	40.0	30.0	41.0
28	35.94790302110242	38.0	34.0	40.0	30.0	41.0
29	35.67354301081851	38.0	34.0	40.0	29.0	41.0
30	35.68004799157699	38.0	34.0	40.0	29.0	41.0
31	35.53280036565011	38.0	34.0	40.0	29.0	41.0
32	35.41221744755002	38.0	34.0	40.0	28.0	41.0
33	35.43221801887832	38.0	34.0	40.0	29.0	41.0
34	35.36048775112939	38.0	34.0	40.0	28.0	41.0
35	35.28338291646772	38.0	34.0	40.0	27.0	41.0
36	35.19508820900821	38.0	34.0	40.0	27.0	41.0
37	35.10461429218545	38.0	34.0	40.0	27.0	41.0
38	35.009990083373125	37.0	34.0	40.0	27.0	41.0
39	34.907763127287865	37.0	34.0	40.0	26.0	41.0
40	34.7130013915925	37.0	33.0	40.0	25.0	41.0
41	34.730745216145735	37.0	33.0	40.0	25.0	41.0
42	34.71301771525814	37.0	33.0	40.0	25.0	41.0
43	34.73028815350775	37.0	33.0	40.0	26.0	41.0
44	34.59259395289806	37.0	33.0	40.0	25.0	41.0
45	34.403463881849305	37.0	33.0	40.0	24.0	41.0
46	34.29610313291953	37.0	33.0	40.0	24.0	41.0
47	34.159604640818145	37.0	33.0	40.0	23.0	41.0
48	34.127047089694464	36.0	33.0	40.0	23.0	41.0
49	33.97417188003738	36.0	33.0	40.0	23.0	41.0
50	33.74792587423432	36.0	33.0	39.0	23.0	40.0
51	31.674910117816058	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	3.0
12	6.0
13	4.0
14	6.0
15	24.0
16	39.0
17	105.0
18	196.0
19	353.0
20	641.0
21	985.0
22	1325.0
23	1776.0
24	2065.0
25	2470.0
26	2812.0
27	3542.0
28	4276.0
29	5489.0
30	7072.0
31	9144.0
32	11750.0
33	16646.0
34	25143.0
35	27671.0
36	28453.0
37	37456.0
38	38005.0
39	17580.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.02464873511996	36.77599441730635	17.718522871496024	25.480833976077665
2	20.861644690931797	36.43768644686851	19.62512701852328	23.07554184367642
3	19.622678468676927	34.458033896091706	21.0563044037169	24.86298323151447
4	19.521471741694317	34.23113494366295	18.65305272952094	27.594340585121795
5	16.079626841003417	38.13453149039148	18.73140632460425	27.05443534400085
6	19.336198136653568	38.372448917128835	21.125679982696916	21.165672963520688
7	73.03167199226259	16.04453095987235	6.30705631256555	4.616740735299519
8	79.80232040907106	7.885962871822497	5.149300326881404	7.162416392225038
9	70.07423186950862	16.321625184151355	9.08575229653571	4.51839064980432
10	35.88921128128533	38.27165028178728	11.775484302755027	14.06365413417237
11	27.836338928269733	27.101773974363685	28.86146513060973	16.200421966756856
12	27.684936929436876	26.189281064955942	29.706214827601684	16.419567178005494
13	22.109588929289963	35.689246377166455	24.50875968707533	17.692405006468253
14	15.841709414266067	37.601971898809595	30.557902082491644	15.998416604432691
15	14.874940316597495	32.397171108744175	32.72160396338602	20.006284611272307
16	17.55038911537975	29.586643976771427	32.666103500202006	20.19686340764682
17	16.50730688083316	29.56991221948801	27.722481360414296	26.200299539264538
18	19.49045677697384	29.341380900495018	26.70061989120277	24.46754243132838
19	19.799790240896495	33.62430267340834	26.80223470982644	19.773672375868724
20	22.842521516631777	32.80811939129051	24.835232999922464	19.514126092155255
21	19.295797064188736	32.468179054288434	25.37554633268447	22.860477548838368
22	19.60717098631669	30.64931461008884	26.009312651248965	23.734201752345506
23	20.174826459029642	32.04049901445868	22.331182690384953	25.45349183612672
24	16.41262962010749	33.98546377574549	27.5694469950172	22.032459609129827
25	18.34086262411087	30.358753361654895	26.758976995874196	24.541407018360044
26	22.050007549695362	35.228919006052	21.319931603840956	21.401141840411682
27	16.384063205233367	33.39740372097958	24.500597854254153	25.7179352195329
28	18.41187056965512	30.87988638728713	28.456638222679288	22.251604820378464
29	20.531090461674072	31.58262019319058	26.547585525805673	21.33870381932967
30	20.420089535306047	30.83907722318124	23.534236848226637	25.206596393286073
31	24.255334777977744	32.624886244455055	22.554816909685236	20.564962067881964
32	22.312002383255184	32.511028676599615	22.049191366413243	23.127777573731958
33	20.75064376456377	32.87790306191158	22.792326244781528	23.57912692874312
34	18.527360504074792	33.618997482074576	23.245716057997985	24.607925955852647
35	18.192725358406484	31.039042127300103	26.36476047061128	24.40347204368213
36	23.31101072056741	30.04125806491106	27.134013214007336	19.513718000514196
37	18.5710263096681	32.33595736258535	25.442881453459187	23.65013487428737
38	19.777345200638255	31.642609664426242	27.450692327469056	21.129352807466447
39	19.015846198422317	32.99053635484384	26.308851915786207	21.68476553094763
40	21.79046126598189	32.07804344543611	23.62442510090066	22.507070187681347
41	19.159086364433996	27.851438318988915	28.72883534726558	24.26063996931151
42	22.975151299975924	27.507417065576245	25.57836787829075	23.939063756157083
43	21.33421481127802	26.647976069506168	27.20910207596218	24.808707043253634
44	21.065690511461256	27.522924547936483	25.452267561203545	25.959117379398716
45	23.03228412972417	30.787657676407814	24.791567194329158	21.388490999538856
46	20.034034842864312	29.546650995947648	28.91247658574209	21.506837575445942
47	20.879600723138388	30.162869373946616	23.719918544908445	25.237611358006557
48	20.94285492750252	26.563501099806974	30.966809906832676	21.526834065857827
49	21.659055757560917	24.458156323584024	29.201813559252866	24.680974359602192
50	19.888346127006283	27.75063968364736	24.09373048811841	28.267283701227946
51	19.075835669657977	27.402129422183048	29.5948058095926	23.927229098566375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3828.0
1	2973.5
2	2119.0
3	1395.0
4	671.0
5	606.5
6	542.0
7	577.5
8	613.0
9	651.0
10	689.0
11	746.0
12	803.0
13	822.5
14	842.0
15	816.5
16	791.0
17	801.0
18	811.0
19	876.0
20	941.0
21	964.0
22	987.0
23	999.5
24	1012.0
25	1151.5
26	1385.5
27	1480.0
28	1845.5
29	2211.0
30	3015.0
31	3819.0
32	3882.5
33	3946.0
34	4606.0
35	5266.0
36	5872.0
37	6478.0
38	7528.0
39	8578.0
40	10910.0
41	13242.0
42	19453.5
43	25665.0
44	27066.0
45	28467.0
46	28529.0
47	28591.0
48	25773.5
49	22956.0
50	21029.0
51	19102.0
52	17601.5
53	16101.0
54	14035.0
55	11969.0
56	10304.5
57	8640.0
58	7903.5
59	7167.0
60	6014.0
61	4861.0
62	4179.0
63	3497.0
64	2843.0
65	2189.0
66	1926.0
67	1663.0
68	1349.0
69	1035.0
70	839.5
71	644.0
72	588.0
73	532.0
74	471.5
75	412.5
76	414.0
77	247.0
78	80.0
79	75.5
80	71.0
81	41.5
82	12.0
83	9.0
84	6.0
85	6.5
86	7.0
87	4.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	245043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.291295813387855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.977220933648	31.82094571156899
2	9.111626540802789	7.34238480593202
3	3.299875419067973	3.9886876997098466
4	1.7319788111130243	2.7913468248429867
5	1.0381744335619005	2.0914696604269456
6	0.720138558304889	1.7409189407573367
7	0.5216193495457354	1.471170366017393
8	0.4335011293312131	1.397305778985729
9	0.3393057904812065	1.2303962977926324
>10	3.4821889781325015	30.31223091457418
>50	0.29474025382098834	7.3089212913651895
>100	0.041526977342476026	3.276975877703097
>500	0.003038559317742148	0.891272144072673
>1k	0.005064265529570247	4.335973686250984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3300	1.3467024154944234	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1998	0.8153670988357146	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT	1818	0.7419106034451096	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	1814	0.740278236880874	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	1695	0.691715331594863	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	781	0.31871957166701353	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	713	0.29096934007500724	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	690	0.2815832323306522	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT	464	0.18935452145133713	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT	425	0.17343894745003938	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	384	0.15670719016662382	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	348	0.14201589108850282	No Hit
AACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	331	0.13507833319050125	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	323	0.13181360006202994	No Hit
TACTAGGGATCTTTGCTTATATTTGACATTGAGCAGTCAGGGGATTTAGGC	304	0.12405985888191051	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	268	0.10936855980378953	No Hit
GATAGAGGGAAAGGATAAAAAGGAAGGAAGGAAGGATGAAGAAAGGAAGAA	267	0.10896046816273061	No Hit
AGAAAAGTAGGTTAAGTTGACGGCCGTTATAAAAATCCTTCGACTGGCGCA	263	0.10732810159849496	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	250	0.10202291026472905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0479793342392967	0.0
2	0.0	0.0	0.0	1.349150965340777	0.0
3	0.0	0.0	0.0	1.8441661259452422	0.0
4	0.0	0.0	0.0	4.4029007153846464	0.0
5	0.0	0.0	0.0	4.7693670090555536	0.0
6	0.0	0.0	0.0	6.349089751594618	0.0
7	0.0	0.0	0.0	7.576629407899838	0.0
8	0.0	0.0	0.0	8.25365344041658	0.0
9	0.0	0.0	0.0	10.113327048722061	0.0
10	4.080916410589162E-4	0.0	0.0	11.741204604906077	0.0
11	4.080916410589162E-4	0.0	0.0	14.257905755316413	0.0
12	4.080916410589162E-4	0.0	0.0	15.216513020163807	0.0
13	4.080916410589162E-4	0.0	0.0	15.663373367123322	0.0
14	4.080916410589162E-4	0.0	0.0	16.146961961778135	0.0
15	4.080916410589162E-4	0.0	0.0	16.502409781140454	0.0
16	4.080916410589162E-4	0.0	0.0	17.148418848936718	0.0
17	4.080916410589162E-4	0.0	0.0	18.037242443163038	0.0
18	4.080916410589162E-4	0.0	0.0	19.166840105614117	0.0
19	4.080916410589162E-4	0.0	0.0	19.701848247042356	0.0
20	4.080916410589162E-4	0.0	0.0	20.177275008875995	0.0
21	4.080916410589162E-4	0.0	0.0	20.734320098921415	0.0
22	4.080916410589162E-4	0.0	0.0	21.321563970405194	0.0
23	4.080916410589162E-4	0.0	0.0	21.91125639173533	0.0
24	4.080916410589162E-4	0.0	0.0	22.36178956346437	0.0
25	4.080916410589162E-4	0.0	0.0	22.726215398929984	0.0
26	4.080916410589162E-4	0.0	0.0	23.054729169982412	0.0
27	4.080916410589162E-4	0.0	0.0	23.46200462775921	0.0
28	4.080916410589162E-4	0.0	0.0	23.86805581061283	0.0
29	4.080916410589162E-4	0.0	0.0	24.2716584436201	0.0
30	4.080916410589162E-4	0.0	0.0	24.882571630285298	0.0
31	4.080916410589162E-4	0.0	0.0	25.283317621805153	0.0
32	4.080916410589162E-4	0.0	0.0	25.634276433115822	0.0
33	4.080916410589162E-4	0.0	0.0	26.03012532494297	0.0
34	4.080916410589162E-4	0.0	0.0	26.465151014311775	0.0
35	4.080916410589162E-4	0.0	0.0	26.923846018861994	0.0
36	4.080916410589162E-4	0.0	0.0	27.333978118126208	0.0
37	4.080916410589162E-4	0.0	0.0	27.71676807743947	0.0
38	4.080916410589162E-4	0.0	0.0	28.13832674265333	0.0
39	4.080916410589162E-4	0.0	0.0	28.541929375660597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	95	0.0	45.000004	3
ATCCGGG	45	3.8198777E-10	45.000004	3
AGTGCGC	45	3.8198777E-10	45.000004	13
CAGGACG	45	3.8198777E-10	45.000004	1
GCTACGG	45	3.8198777E-10	45.000004	2
CTTAATG	40	6.7775545E-9	45.0	45
TCGCCGG	20	7.0224126E-4	45.0	32
CGGGTCA	40	6.7775545E-9	45.0	6
GAGTGCG	20	7.0224126E-4	45.0	40
CGGAACG	20	7.0224126E-4	45.0	1
TTGGCGG	20	7.0224126E-4	45.0	2
AGTCCGG	40	6.7775545E-9	45.0	2
AAATCGC	20	7.0224126E-4	45.0	45
TGCCCCG	20	7.0224126E-4	45.0	1
GCGACGG	50	2.1827873E-11	45.0	2
AATCAGC	20	7.0224126E-4	45.0	23
ACGCATG	25	3.8817932E-5	45.0	1
CTATCGG	35	1.2066994E-7	45.0	2
CGTGCGG	20	7.0224126E-4	45.0	2
CCAGGTG	20	7.0224126E-4	45.0	20
>>END_MODULE