SRR3553556_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369475
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2977	0.8057378713038771	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2849	0.7710941200351851	Illumina PCR Primer Index 5 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1130	0.30583936666892214	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	959	0.25955748020840386	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	864	0.23384532106367142	No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	746	0.20190811286284593	Illumina PCR Primer Index 5 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	655	0.17727857094526017	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.15508491778875433	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	521	0.14101089383584817	No Hit
ACACAAGGGAAAGTTGCCCTGTGACCGAGTTAGTGTGCTTTTCAACATATA	397	0.10744975979430274	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.1025779822721429	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	30	2.1614887E-6	45.000004	1
GGATCGA	30	2.1614887E-6	45.000004	8
AATATAC	30	2.1614887E-6	45.000004	15
CATAGCG	30	2.1614887E-6	45.000004	1
CTACGAA	30	2.1614887E-6	45.000004	10
TACGAAG	30	2.1614887E-6	45.000004	1
CGAATAT	30	2.1614887E-6	45.000004	13
TACTACG	55	1.8189894E-12	45.000004	1
TTCGCAG	30	2.1614887E-6	45.000004	1
GTCATAC	20	7.0270663E-4	45.0	39
ACGTTAG	25	3.8856502E-5	45.0	1
GCGGTAG	20	7.0270663E-4	45.0	16
CGTTGGT	20	7.0270663E-4	45.0	43
CGTTGGG	20	7.0270663E-4	45.0	3
ACCGAGT	50	2.1827873E-11	45.0	24
TATGCGA	20	7.0270663E-4	45.0	11
GCTTGCG	20	7.0270663E-4	45.0	1
TTATCCG	20	7.0270663E-4	45.0	1
ATACTCC	20	7.0270663E-4	45.0	12
CTACGGC	20	7.0270663E-4	45.0	3