##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369475
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.918603423776982	31.0	31.0	33.0	28.0	34.0
2	31.10324108532377	31.0	31.0	34.0	28.0	34.0
3	31.344605183029977	31.0	31.0	34.0	28.0	34.0
4	35.36037620948643	37.0	35.0	37.0	33.0	37.0
5	34.942946072129374	35.0	35.0	37.0	32.0	37.0
6	34.989761147574264	35.0	35.0	37.0	32.0	37.0
7	34.023238378780704	36.0	35.0	37.0	31.0	37.0
8	34.81032004871778	37.0	35.0	37.0	32.0	37.0
9	36.09136748088504	39.0	35.0	39.0	32.0	39.0
10	35.54108938358482	37.0	35.0	39.0	30.0	39.0
11	35.93079910684079	37.0	35.0	39.0	31.0	39.0
12	35.98713038771229	37.0	35.0	39.0	31.0	39.0
13	35.854355504431965	37.0	35.0	39.0	30.0	39.0
14	36.8895513904865	39.0	36.0	40.0	31.0	41.0
15	36.95412680154273	38.0	36.0	40.0	32.0	41.0
16	36.90790175248664	38.0	35.0	40.0	32.0	41.0
17	36.88590297043102	38.0	35.0	40.0	32.0	41.0
18	36.69609310508154	38.0	35.0	40.0	31.0	41.0
19	36.740464172136136	38.0	35.0	40.0	31.0	41.0
20	36.68972190269977	38.0	35.0	40.0	31.0	41.0
21	36.756172948102034	38.0	35.0	40.0	31.0	41.0
22	36.728839569659655	38.0	35.0	40.0	31.0	41.0
23	36.665330536572164	38.0	35.0	40.0	31.0	41.0
24	36.329229311861425	38.0	35.0	40.0	30.0	41.0
25	36.449372758644024	38.0	35.0	40.0	30.0	41.0
26	36.3464564584884	38.0	35.0	40.0	30.0	41.0
27	36.22006089721903	38.0	35.0	40.0	30.0	41.0
28	36.127513363556396	38.0	35.0	40.0	30.0	41.0
29	35.89668854455647	38.0	35.0	40.0	29.0	41.0
30	35.78161445293998	38.0	34.0	40.0	29.0	41.0
31	35.69109682657825	38.0	34.0	40.0	29.0	41.0
32	35.59254076730496	38.0	34.0	40.0	28.0	41.0
33	35.561937884836595	38.0	34.0	40.0	28.0	41.0
34	35.566704107179106	38.0	34.0	40.0	28.0	41.0
35	35.52932945395494	38.0	34.0	40.0	28.0	41.0
36	35.364641721361394	38.0	34.0	40.0	27.0	41.0
37	35.257906488937	38.0	34.0	40.0	27.0	41.0
38	35.208000541308614	38.0	34.0	40.0	27.0	41.0
39	35.079082481899995	38.0	34.0	40.0	26.0	41.0
40	34.9215670884363	38.0	34.0	40.0	25.0	41.0
41	34.96357804993572	38.0	34.0	40.0	25.0	41.0
42	34.89797685905677	38.0	34.0	40.0	25.0	41.0
43	34.8309790919548	38.0	34.0	40.0	25.0	41.0
44	34.67898233980648	38.0	33.0	40.0	24.0	41.0
45	34.48925367074904	37.0	33.0	40.0	24.0	41.0
46	34.42443196427363	37.0	33.0	40.0	23.0	41.0
47	34.27734217470736	37.0	33.0	40.0	23.0	41.0
48	34.2277447729887	37.0	33.0	40.0	23.0	41.0
49	34.02291630015563	37.0	33.0	40.0	23.0	41.0
50	33.86747953176805	37.0	33.0	40.0	22.0	41.0
51	31.88723188307734	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	6.0
12	9.0
13	11.0
14	10.0
15	32.0
16	56.0
17	143.0
18	287.0
19	561.0
20	936.0
21	1491.0
22	2219.0
23	2776.0
24	3417.0
25	3860.0
26	4378.0
27	5260.0
28	6522.0
29	8073.0
30	9952.0
31	12458.0
32	15979.0
33	21610.0
34	31580.0
35	35940.0
36	40736.0
37	55206.0
38	68261.0
39	37700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.215711482508965	33.21875634346031	21.178428851749104	25.387103322281618
2	23.45679680627918	32.434941471006155	20.328574328439	23.779687394275662
3	20.639826781243656	33.81933825021991	19.720143446782597	25.82069152175384
4	19.916909127816496	33.733811489275325	18.953109141349213	27.39617024155897
5	17.421747073550307	36.64957033628798	17.739495229717843	28.189187360443874
6	18.966641856688547	39.15339332837134	19.355030786927397	22.524934028012723
7	80.78435618106774	9.922186886798837	4.901278841599567	4.392178090533866
8	84.99032410853238	6.233439339603492	4.063333107788078	4.712903444076054
9	77.41065024697205	11.275999729345692	7.470870830232086	3.8424791934501656
10	32.95703362879762	42.94011773462345	10.88923472494756	13.21361391163137
11	22.969077745449624	28.314229650179307	31.498206915217537	17.21848568915353
12	25.741931118478924	26.081060964882603	31.3063130117058	16.870694904932677
13	24.50828878814534	31.408079031057586	25.986331957507275	18.097300223289803
14	18.422356045740578	34.57500507476825	29.051221327559375	17.951417551931794
15	16.15535557209554	32.62521144867718	31.7504567291427	19.46897625008458
16	18.845118072941336	31.402124636308276	29.81473712700453	19.938020163745858
17	18.393125380607618	28.730495974017185	28.254956356993034	24.621422288382163
18	21.42228838216388	28.459841667230528	27.739089248257663	22.378780702347925
19	21.314838622369578	32.33642330333581	26.54198524934028	19.806752824954327
20	22.611272751877664	31.58535760200284	25.364639014818323	20.438730631301173
21	20.897489681304553	31.903647066783954	26.353068543203197	20.8457947087083
22	20.313688341565737	30.034508424115298	25.501048785438797	24.150754448880168
23	19.786183097638542	32.01759252994113	23.55071385073415	24.645510521686177
24	17.082617227146628	32.68231950740916	28.1886460518303	22.04641721361391
25	19.647337438256987	30.741728127748836	26.40097435550443	23.20996007848975
26	20.804384599769943	33.06448338859192	23.105758170376888	23.02537384126125
27	18.153325664794643	32.36700724000271	25.79227281954124	23.68739427566141
28	20.7139860613032	30.014209351106295	29.051491981866164	20.220312605724338
29	19.854658637255564	29.986873266120845	26.953650449962787	23.204817646660803
30	21.881859395087623	32.09716489613641	25.291021043372353	20.729954665403614
31	23.521212531294406	30.885716218959335	23.960755125515934	21.63231612423033
32	23.79349076392178	30.67893632857433	24.50991271398606	21.017660193517827
33	21.643954259422152	30.622640232762706	25.533797956559983	22.19960755125516
34	21.119155558562824	28.69152175383991	26.9452601664524	23.24406252114487
35	20.929156235198594	29.80851207794844	27.096014615332564	22.1663170715204
36	21.975235130929022	29.282630759861966	28.727518776642537	20.01461533256648
37	19.449218485689155	32.10907368563502	27.23919074362271	21.202517085053117
38	21.537857771161782	30.074565261519727	27.7342174707355	20.65335949658299
39	21.54868394343325	28.07551255159348	27.94505717572231	22.430746329250965
40	22.61966303538805	28.391366127613505	26.98396373232289	22.005007104675553
41	20.282292441978484	24.99438392313418	31.30062927126328	23.42269436362406
42	23.596454428581097	26.89924893429867	26.31544759455985	23.18884904256039
43	20.099871439204275	26.953109141349213	27.939644089586572	25.007375329859936
44	21.441234183638947	26.866229108870694	26.8012720752419	24.89126463224846
45	23.16449015494959	28.644969213072603	26.33087489004669	21.85966574193112
46	21.2839840313959	27.821638811827594	28.97841531903376	21.915961837742742
47	20.480411394546316	28.024900196224372	26.70221259895798	24.79247581027133
48	20.54076730495974	26.176601935178294	31.494417754922527	21.78821300493944
49	21.789295622166588	24.925908383517154	29.733270180661748	23.551525813654507
50	20.440083902835106	26.003924487448405	27.733405507815146	25.822586101901347
51	20.40083902835104	25.656133703227553	30.54117328641992	23.401853982001487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3534.0
1	2753.0
2	1972.0
3	1362.5
4	753.0
5	715.0
6	677.0
7	760.0
8	843.0
9	898.5
10	954.0
11	1088.5
12	1223.0
13	1261.0
14	1299.0
15	1333.0
16	1367.0
17	1300.5
18	1234.0
19	1198.5
20	1163.0
21	1171.5
22	1180.0
23	1357.5
24	1535.0
25	1849.0
26	2732.5
27	3302.0
28	3747.0
29	4192.0
30	5028.0
31	5864.0
32	7143.0
33	8422.0
34	8767.0
35	9112.0
36	10220.5
37	11329.0
38	12674.0
39	14019.0
40	17463.5
41	20908.0
42	26591.5
43	32275.0
44	34730.0
45	37185.0
46	39465.0
47	41745.0
48	39331.0
49	36917.0
50	33881.0
51	30845.0
52	27351.5
53	23858.0
54	20730.0
55	17602.0
56	15854.0
57	14106.0
58	12361.0
59	10616.0
60	9637.0
61	8658.0
62	7175.0
63	5692.0
64	4678.5
65	3665.0
66	3253.0
67	2841.0
68	2307.0
69	1773.0
70	1541.0
71	1309.0
72	1263.0
73	1217.0
74	1047.0
75	703.5
76	530.0
77	425.0
78	320.0
79	233.0
80	146.0
81	126.5
82	107.0
83	86.0
84	65.0
85	48.5
86	32.0
87	28.0
88	24.0
89	15.0
90	6.0
91	4.5
92	3.0
93	6.5
94	10.0
95	6.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	369475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.954379171833594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97503599992854	31.953529126221863
2	8.458378238772116	6.758985026613744
3	2.8779773386875225	3.449633935135975
4	1.3876411513022102	2.2176936285427282
5	0.8920055393467151	1.781976377121729
6	0.6673991303343206	1.5999310747397661
7	0.5308525003957411	1.4846917459589166
8	0.4310262203057671	1.377710803127912
9	0.38725426756645	1.3925253458034579
>10	4.09249111016909	35.84718816257066
>50	0.2549812778809375	6.392998803231807
>100	0.0389181950449852	2.720630372188056
>500	0.004026020177067434	1.1576351938419418
>1k	0.002013010088533717	1.8648704049014697
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2977	0.8057378713038771	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2849	0.7710941200351851	Illumina PCR Primer Index 5 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1130	0.30583936666892214	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	959	0.25955748020840386	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	864	0.23384532106367142	No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	746	0.20190811286284593	Illumina PCR Primer Index 5 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	655	0.17727857094526017	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.15508491778875433	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	521	0.14101089383584817	No Hit
ACACAAGGGAAAGTTGCCCTGTGACCGAGTTAGTGTGCTTTTCAACATATA	397	0.10744975979430274	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.1025779822721429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.413086135733135E-4	0.0	0.0	0.9762500845794708	0.0
2	5.413086135733135E-4	0.0	0.0	1.2374314906285946	0.0
3	5.413086135733135E-4	0.0	0.0	1.6239258407199404	0.0
4	5.413086135733135E-4	0.0	0.0	3.389674538196089	0.0
5	5.413086135733135E-4	0.0	0.0	3.734217470735503	0.0
6	5.413086135733135E-4	0.0	0.0	5.141078557412545	0.0
7	5.413086135733135E-4	0.0	0.0	6.210163069219839	0.0
8	5.413086135733135E-4	0.0	0.0	6.980986534948237	0.0
9	5.413086135733135E-4	0.0	0.0	8.331551525813655	0.0
10	5.413086135733135E-4	0.0	0.0	9.827457879423505	0.0
11	5.413086135733135E-4	0.0	0.0	11.589146762297855	0.0
12	5.413086135733135E-4	0.0	0.0	12.41274781784965	0.0
13	5.413086135733135E-4	0.0	0.0	12.80303132823601	0.0
14	5.413086135733135E-4	0.0	0.0	13.17220380269301	0.0
15	5.413086135733135E-4	0.0	0.0	13.446376615467894	0.0
16	5.413086135733135E-4	0.0	0.0	14.032072535354219	0.0
17	5.413086135733135E-4	0.0	0.0	14.83591582651059	0.0
18	5.413086135733135E-4	0.0	0.0	15.82434535489546	0.0
19	5.413086135733135E-4	0.0	0.0	16.27606739292239	0.0
20	5.413086135733135E-4	0.0	0.0	16.742946072129374	0.0
21	5.413086135733135E-4	0.0	0.0	17.232289058799648	0.0
22	5.413086135733135E-4	0.0	0.0	17.806617497800932	0.0
23	5.413086135733135E-4	0.0	0.0	18.39068949184654	0.0
24	5.413086135733135E-4	0.0	0.0	18.814534136274442	0.0
25	5.413086135733135E-4	0.0	0.0	19.172068475539618	0.0
26	5.413086135733135E-4	0.0	0.0	19.525272345896205	0.0
27	5.413086135733135E-4	0.0	0.0	19.89715136342107	0.0
28	5.413086135733135E-4	0.0	0.0	20.279585898910618	0.0
29	5.413086135733135E-4	0.0	0.0	20.69043913661276	0.0
30	5.413086135733135E-4	0.0	0.0	21.226064009743556	0.0
31	5.413086135733135E-4	0.0	0.0	21.624196495026727	0.0
32	5.413086135733135E-4	0.0	0.0	22.00852561066378	0.0
33	5.413086135733135E-4	0.0	0.0	22.39880912105014	0.0
34	5.413086135733135E-4	0.0	0.0	22.798294877867242	0.0
35	5.413086135733135E-4	0.0	0.0	23.238378780702348	0.0
36	5.413086135733135E-4	0.0	0.0	23.626497056634413	0.0
37	5.413086135733135E-4	0.0	0.0	24.00162392584072	0.0
38	5.413086135733135E-4	0.0	0.0	24.38865958454564	0.0
39	5.413086135733135E-4	0.0	0.0	24.787604032749172	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	30	2.1614887E-6	45.000004	1
GGATCGA	30	2.1614887E-6	45.000004	8
AATATAC	30	2.1614887E-6	45.000004	15
CATAGCG	30	2.1614887E-6	45.000004	1
CTACGAA	30	2.1614887E-6	45.000004	10
TACGAAG	30	2.1614887E-6	45.000004	1
CGAATAT	30	2.1614887E-6	45.000004	13
TACTACG	55	1.8189894E-12	45.000004	1
TTCGCAG	30	2.1614887E-6	45.000004	1
GTCATAC	20	7.0270663E-4	45.0	39
ACGTTAG	25	3.8856502E-5	45.0	1
GCGGTAG	20	7.0270663E-4	45.0	16
CGTTGGT	20	7.0270663E-4	45.0	43
CGTTGGG	20	7.0270663E-4	45.0	3
ACCGAGT	50	2.1827873E-11	45.0	24
TATGCGA	20	7.0270663E-4	45.0	11
GCTTGCG	20	7.0270663E-4	45.0	1
TTATCCG	20	7.0270663E-4	45.0	1
ATACTCC	20	7.0270663E-4	45.0	12
CTACGGC	20	7.0270663E-4	45.0	3
>>END_MODULE