FastQCFastQC Report
Sat 18 Jun 2016
SRR3553555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431611
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36440.8442787602725602No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT30170.699009061400196Illumina PCR Primer Index 5 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT11460.2655168658815461Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC9680.22427602632926405No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT9400.21778870325362423No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.18651053842464627No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT7940.18396194721635917Illumina PCR Primer Index 5 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.17029223073554658No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT5800.13438026370968303No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.1031020988807051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG351.2095734E-745.0000041
GCGGCTA351.2095734E-745.00000423
CCGAACG351.2095734E-745.0000041
TGGGACG351.2095734E-745.0000046
GTGCGAC351.2095734E-745.00000445
GACTACG351.2095734E-745.0000041
CGGGTAT253.8867445E-545.06
AAGTACG207.0283865E-445.01
ACAGTCG207.0283865E-445.01
CGTATAT207.0283865E-445.039
CAACGGA207.0283865E-445.03
CGCGGAG253.8867445E-545.04
ACGTTAG207.0283865E-445.01
CGTGAGT207.0283865E-445.032
ATTGTCG207.0283865E-445.034
ATGCTCG207.0283865E-445.01
TTCAACG207.0283865E-445.01
ATATGCG207.0283865E-445.01
TATGCGA253.8867445E-545.011
ACCCTAC207.0283865E-445.022