Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553555_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 431611 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3644 | 0.8442787602725602 | No Hit |
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT | 3017 | 0.699009061400196 | Illumina PCR Primer Index 5 (95% over 24bp) |
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT | 1146 | 0.2655168658815461 | Illumina PCR Primer Index 5 (95% over 21bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC | 968 | 0.22427602632926405 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT | 940 | 0.21778870325362423 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 805 | 0.18651053842464627 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT | 794 | 0.18396194721635917 | Illumina PCR Primer Index 5 (95% over 21bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 735 | 0.17029223073554658 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT | 580 | 0.13438026370968303 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 445 | 0.1031020988807051 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CAGCGCG | 35 | 1.2095734E-7 | 45.000004 | 1 |
GCGGCTA | 35 | 1.2095734E-7 | 45.000004 | 23 |
CCGAACG | 35 | 1.2095734E-7 | 45.000004 | 1 |
TGGGACG | 35 | 1.2095734E-7 | 45.000004 | 6 |
GTGCGAC | 35 | 1.2095734E-7 | 45.000004 | 45 |
GACTACG | 35 | 1.2095734E-7 | 45.000004 | 1 |
CGGGTAT | 25 | 3.8867445E-5 | 45.0 | 6 |
AAGTACG | 20 | 7.0283865E-4 | 45.0 | 1 |
ACAGTCG | 20 | 7.0283865E-4 | 45.0 | 1 |
CGTATAT | 20 | 7.0283865E-4 | 45.0 | 39 |
CAACGGA | 20 | 7.0283865E-4 | 45.0 | 3 |
CGCGGAG | 25 | 3.8867445E-5 | 45.0 | 4 |
ACGTTAG | 20 | 7.0283865E-4 | 45.0 | 1 |
CGTGAGT | 20 | 7.0283865E-4 | 45.0 | 32 |
ATTGTCG | 20 | 7.0283865E-4 | 45.0 | 34 |
ATGCTCG | 20 | 7.0283865E-4 | 45.0 | 1 |
TTCAACG | 20 | 7.0283865E-4 | 45.0 | 1 |
ATATGCG | 20 | 7.0283865E-4 | 45.0 | 1 |
TATGCGA | 25 | 3.8867445E-5 | 45.0 | 11 |
ACCCTAC | 20 | 7.0283865E-4 | 45.0 | 22 |