##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431611
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941044134649026	31.0	31.0	33.0	28.0	34.0
2	31.124584405865466	31.0	31.0	34.0	28.0	34.0
3	31.37373931618981	31.0	31.0	34.0	28.0	34.0
4	35.373743949992004	37.0	35.0	37.0	33.0	37.0
5	35.00417505577939	35.0	35.0	37.0	32.0	37.0
6	35.02464719388524	36.0	35.0	37.0	32.0	37.0
7	32.80987277896068	35.0	35.0	37.0	27.0	37.0
8	34.24186825636974	37.0	35.0	37.0	30.0	37.0
9	34.469283683687394	38.0	35.0	39.0	28.0	39.0
10	34.77692876224192	37.0	34.0	39.0	26.0	39.0
11	35.66252250290192	37.0	35.0	39.0	30.0	39.0
12	35.93091695994773	37.0	35.0	39.0	31.0	39.0
13	35.91561151129142	37.0	35.0	39.0	31.0	39.0
14	36.97763031989454	39.0	36.0	40.0	32.0	41.0
15	37.04468143768347	39.0	36.0	40.0	32.0	41.0
16	37.01940172979836	38.0	36.0	40.0	32.0	41.0
17	36.98659672714551	38.0	36.0	40.0	32.0	41.0
18	36.8442162039429	38.0	35.0	40.0	31.0	41.0
19	36.846922344425884	39.0	35.0	40.0	31.0	41.0
20	36.769148608353355	38.0	35.0	40.0	31.0	41.0
21	36.83462655029645	38.0	35.0	40.0	31.0	41.0
22	36.835432831878705	39.0	35.0	40.0	31.0	41.0
23	36.786034183558805	38.0	35.0	40.0	31.0	41.0
24	36.454837805338606	38.0	35.0	40.0	30.0	41.0
25	36.561125643229666	38.0	35.0	40.0	31.0	41.0
26	36.47907954153161	38.0	35.0	40.0	31.0	41.0
27	36.34951843210669	38.0	35.0	40.0	30.0	41.0
28	36.252646480279694	38.0	35.0	40.0	30.0	41.0
29	35.937916318166124	38.0	35.0	40.0	29.0	41.0
30	35.83980714115257	38.0	35.0	40.0	29.0	41.0
31	35.79313085162334	38.0	34.0	40.0	29.0	41.0
32	35.7595659054102	38.0	34.0	40.0	29.0	41.0
33	35.71427048893564	38.0	34.0	40.0	29.0	41.0
34	35.69545956891738	38.0	34.0	40.0	29.0	41.0
35	35.5948504556186	38.0	34.0	40.0	28.0	41.0
36	35.455062544745154	38.0	34.0	40.0	27.0	41.0
37	35.339958898174515	38.0	34.0	40.0	27.0	41.0
38	35.29517088304051	38.0	34.0	40.0	27.0	41.0
39	35.22471623753797	38.0	34.0	40.0	26.0	41.0
40	35.081724052445374	38.0	34.0	40.0	26.0	41.0
41	35.07892755281955	38.0	34.0	40.0	26.0	41.0
42	34.98579739626655	38.0	34.0	40.0	26.0	41.0
43	34.917476616675664	38.0	34.0	40.0	25.0	41.0
44	34.729017564427224	38.0	33.0	40.0	24.0	41.0
45	34.538901927893406	37.0	33.0	40.0	24.0	41.0
46	34.49686870816546	37.0	33.0	40.0	24.0	41.0
47	34.39267303196628	37.0	33.0	40.0	23.0	41.0
48	34.35199983318312	37.0	33.0	40.0	23.0	41.0
49	34.13496180588539	37.0	33.0	40.0	23.0	41.0
50	33.90950879379812	37.0	33.0	40.0	22.0	41.0
51	32.01236066736019	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	6.0
12	6.0
13	12.0
14	10.0
15	31.0
16	77.0
17	148.0
18	289.0
19	572.0
20	1037.0
21	1715.0
22	2538.0
23	3190.0
24	3814.0
25	4331.0
26	5067.0
27	6034.0
28	7323.0
29	9218.0
30	11591.0
31	14500.0
32	19251.0
33	25227.0
34	37385.0
35	43142.0
36	50050.0
37	68063.0
38	76059.0
39	40919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.23048532127309	33.08279909455505	21.248068283709173	25.438647300462687
2	23.56543276237167	32.25288512109283	20.28238390587821	23.89929821065728
3	20.657721883825943	33.68866873179785	19.694586097203267	25.95902328717294
4	19.821783967507777	33.63074620433677	19.03218407315847	27.515285754996977
5	17.400854009744886	36.75902606745426	17.7242934030875	28.115826519713355
6	19.04029322700302	39.161652506539454	19.317626288486622	22.480427977970905
7	78.03786279775076	13.147023593003885	4.651874025453476	4.163239583791887
8	85.21909775237424	6.261193528431852	3.9922522827268074	4.527456436467096
9	74.57270551491968	14.767695911364632	7.0890222909054685	3.5705762828102157
10	32.93266390337596	43.030182270609416	10.830354184670918	13.20679964134371
11	23.077493391039617	28.4441314053627	31.481125365201535	16.997249838396147
12	25.78734091577833	26.25535493766378	31.150966958673436	16.80633718788446
13	24.396273496273267	31.400265516865883	26.194420438774728	18.009040548086126
14	18.521539071061675	34.66825451621946	28.878318671210884	17.93188774150798
15	16.28572951106436	32.60181042651833	31.83746475414204	19.274995308275276
16	19.034037594037223	31.420422556422334	29.758972778728992	19.786567070811447
17	18.44716654580166	28.843333464624394	28.230744814196118	24.478755175377827
18	21.40167882653593	28.615582086647468	27.603559686847646	22.379179399968955
19	21.38800911005512	32.3191484925083	26.58111123210484	19.711731165331745
20	22.819853988892778	31.34651341138201	25.44652476419739	20.387107835527825
21	20.930652833222506	31.917166151928473	26.29752253765544	20.85465847719358
22	20.32710009707816	29.88802416991226	25.528079682862575	24.25679605014701
23	19.80556565981868	32.04366895190345	23.71950668541812	24.43125870285975
24	17.05748926695566	32.79527166823829	28.25020678342304	21.897032281383005
25	19.650796666442698	30.942909240033273	26.25628169810315	23.150012395420877
26	20.829172565110714	33.12936880663375	23.113173667955635	22.928284960299898
27	18.107045464550257	32.44889495402109	25.835764148735784	23.608295432692863
28	20.59308034317939	30.24853398082996	29.082437657983697	20.075948018006954
29	19.97724803121329	29.796506576523768	26.931194988079543	23.295050404183396
30	21.828683698978942	32.29157736943683	25.276232533461844	20.603506398122384
31	23.553153186549924	30.87363389718983	23.988730593057177	21.58448232320307
32	23.832803149131973	30.86645150378466	24.41504039517065	20.885704951912718
33	21.54926542650674	30.63128604229271	25.668483889428213	22.150964641772337
34	21.173464068339314	28.909133455820175	26.761829517783376	23.15557295805714
35	21.005720428812054	29.600960123815195	27.12257101881092	22.27074842856183
36	22.017279448392184	29.54164745569506	28.572719416326276	19.868353679586477
37	19.358403747819217	32.16878161121936	27.32645831547389	21.146356325487535
38	21.59212809682793	30.269386090715944	27.605644897836246	20.532840914619875
39	21.473039380367968	28.276851146055126	27.857260357127135	22.392849116449767
40	22.644927955960345	28.502749003153305	26.85612739249	21.99619564839636
41	20.35814657179729	25.013495948898427	31.266348633375884	23.362008845928393
42	23.537861639300203	27.18906608033623	26.233576067338415	23.039496213025153
43	20.08544731251057	27.247451988016984	27.911475842830697	24.755624856641745
44	21.456126002349336	27.015761878172707	26.65455699692547	24.873555122552485
45	23.084675784444787	28.725866578933346	26.43931688488014	21.750140751741732
46	21.329159822154672	28.017358223029532	28.764327137167495	21.889154817648297
47	20.45174937617438	28.131118066963072	26.690005583731647	24.727126973130897
48	20.613237382735843	26.408965480490533	31.377096505881454	21.60070063089217
49	21.78327243744946	25.078369179654832	29.785617141361087	23.352741241534623
50	20.3922050179444	26.228710575031684	27.665189256066224	25.71389515095769
51	20.391973327834556	25.906893012457978	30.432495928046322	23.268637731661148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4283.0
1	3323.5
2	2364.0
3	1625.5
4	887.0
5	838.0
6	789.0
7	858.0
8	927.0
9	1069.5
10	1212.0
11	1314.5
12	1417.0
13	1487.5
14	1558.0
15	1576.0
16	1594.0
17	1558.5
18	1523.0
19	1470.5
20	1418.0
21	1428.5
22	1439.0
23	1658.0
24	1877.0
25	2251.5
26	3283.5
27	3941.0
28	4515.5
29	5090.0
30	6029.5
31	6969.0
32	8336.5
33	9704.0
34	10209.0
35	10714.0
36	12219.0
37	13724.0
38	15543.0
39	17362.0
40	21194.5
41	25027.0
42	31414.0
43	37801.0
44	40912.5
45	44024.0
46	46148.0
47	48272.0
48	45430.5
49	42589.0
50	38875.5
51	35162.0
52	31274.5
53	27387.0
54	23730.0
55	20073.0
56	18190.0
57	16307.0
58	14271.0
59	12235.0
60	11039.5
61	9844.0
62	8224.5
63	6605.0
64	5498.5
65	4392.0
66	3856.0
67	3320.0
68	2631.5
69	1943.0
70	1735.5
71	1528.0
72	1434.0
73	1340.0
74	1142.5
75	769.5
76	594.0
77	481.0
78	368.0
79	256.0
80	144.0
81	136.0
82	128.0
83	93.5
84	59.0
85	53.0
86	47.0
87	32.0
88	17.0
89	13.5
90	10.0
91	10.0
92	10.0
93	12.0
94	14.0
95	8.5
96	3.0
97	3.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.04025305059019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18657823656669	30.524237995153307
2	9.271204365526588	7.239003290277888
3	3.3582177849538026	3.9331701637076675
4	1.7727786947662176	2.768389153854725
5	1.0533823622721645	2.0562156991066884
6	0.7599069278571748	1.7800175255064412
7	0.5455974572034672	1.4910183955087337
8	0.4394397249443546	1.3724670449847483
9	0.3720174371226551	1.3071289396050432
>10	3.87656332269535	33.83288578333624
>50	0.31513133846919655	7.700514293397109
>100	0.0437176423309868	3.0012686307126613
>500	0.0036431368609155666	1.1430469423660417
>1k	0.0018215684304577833	1.850636142482722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3644	0.8442787602725602	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	3017	0.699009061400196	Illumina PCR Primer Index 5 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1146	0.2655168658815461	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	968	0.22427602632926405	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	940	0.21778870325362423	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805	0.18651053842464627	No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	794	0.18396194721635917	Illumina PCR Primer Index 5 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	735	0.17029223073554658	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	580	0.13438026370968303	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	445	0.1031020988807051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8913118525709494	0.0
2	0.0	0.0	0.0	1.1313428063696245	0.0
3	0.0	0.0	0.0	1.4652082546552336	0.0
4	0.0	0.0	0.0	3.090977755432554	0.0
5	0.0	0.0	0.0	3.3866143355938565	0.0
6	0.0	0.0	0.0	4.653032476002697	0.0
7	0.0	0.0	0.0	5.640495724159023	0.0
8	0.0	0.0	0.0	6.366380838301155	0.0
9	0.0	0.0	0.0	7.6005940534416405	0.0
10	0.0	0.0	0.0	9.121407934459501	0.0
11	0.0	0.0	0.0	10.813904186871975	0.0
12	0.0	0.0	0.0	11.62644140209587	0.0
13	0.0	0.0	0.0	12.018692758062237	0.0
14	0.0	0.0	0.0	12.383604681066979	0.0
15	0.0	0.0	0.0	12.669510276614822	0.0
16	0.0	0.0	0.0	13.257308085289763	0.0
17	0.0	0.0	0.0	14.052931922495025	0.0
18	0.0	0.0	0.0	15.029737425598514	0.0
19	0.0	0.0	0.0	15.473192295840468	0.0
20	2.3169010984428108E-4	0.0	0.0	15.914561955093824	0.0
21	2.3169010984428108E-4	0.0	0.0	16.40041611543728	0.0
22	2.3169010984428108E-4	0.0	0.0	16.9729223768625	0.0
23	2.3169010984428108E-4	0.0	0.0	17.542184976749898	0.0
24	2.3169010984428108E-4	0.0	0.0	17.93791168436393	0.0
25	2.3169010984428108E-4	0.0	0.0	18.31510318319042	0.0
26	2.3169010984428108E-4	0.0	0.0	18.655919334771358	0.0
27	2.3169010984428108E-4	0.0	0.0	19.024538299533607	0.0
28	2.3169010984428108E-4	0.0	0.0	19.419801626927953	0.0
29	2.3169010984428108E-4	0.0	0.0	19.819003686189646	0.0
30	2.3169010984428108E-4	0.0	0.0	20.370889527838724	0.0
31	2.3169010984428108E-4	0.0	0.0	20.765921165123224	0.0
32	2.3169010984428108E-4	0.0	0.0	21.141490833180804	0.0
33	2.3169010984428108E-4	0.0	0.0	21.530035147389665	0.0
34	2.3169010984428108E-4	0.0	0.0	21.935492839617154	0.0
35	2.3169010984428108E-4	0.0	0.0	22.384739962605217	0.0
36	2.3169010984428108E-4	0.0	0.0	22.77351596692392	0.0
37	4.6338021968856215E-4	0.0	0.0	23.147927184432277	0.0
38	4.6338021968856215E-4	0.0	0.0	23.520021500842194	0.0
39	4.6338021968856215E-4	0.0	0.0	23.930112995266573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	35	1.2095734E-7	45.000004	1
GCGGCTA	35	1.2095734E-7	45.000004	23
CCGAACG	35	1.2095734E-7	45.000004	1
TGGGACG	35	1.2095734E-7	45.000004	6
GTGCGAC	35	1.2095734E-7	45.000004	45
GACTACG	35	1.2095734E-7	45.000004	1
CGGGTAT	25	3.8867445E-5	45.0	6
AAGTACG	20	7.0283865E-4	45.0	1
ACAGTCG	20	7.0283865E-4	45.0	1
CGTATAT	20	7.0283865E-4	45.0	39
CAACGGA	20	7.0283865E-4	45.0	3
CGCGGAG	25	3.8867445E-5	45.0	4
ACGTTAG	20	7.0283865E-4	45.0	1
CGTGAGT	20	7.0283865E-4	45.0	32
ATTGTCG	20	7.0283865E-4	45.0	34
ATGCTCG	20	7.0283865E-4	45.0	1
TTCAACG	20	7.0283865E-4	45.0	1
ATATGCG	20	7.0283865E-4	45.0	1
TATGCGA	25	3.8867445E-5	45.0	11
ACCCTAC	20	7.0283865E-4	45.0	22
>>END_MODULE