FastQCFastQC Report
Sat 18 Jun 2016
SRR3553552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140459
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT35940.3151362740791208Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACA207.0332625E-445.00000429
CGTAGCG453.8562575E-1045.01
ACGGGTA1900.041.447375
GTAACGG2450.041.326532
TAAGACG501.0822987E-940.51
ACGAATA1000.040.512
TCTAGCG850.039.7058831
AATCGTT1650.039.54545222
CTTAACG403.45819E-739.3750041
ACGCATG800.039.3750041
CGTAACG403.45819E-739.3750041
CGAATAG750.039.01
TAGCACG1050.038.571431
CCATCGC356.248247E-638.57142626
ATAAGCG700.038.5714261
AATTACG700.038.5714261
ATACCCG700.038.5714261
TCGAACA356.248247E-638.57142613
GTACGAG356.248247E-638.5714261
ATCGTTG1700.038.38235523
Produced by FastQC (version 0.11.5)