##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140459
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923861357576204	33.0	31.0	34.0	30.0	34.0
2	32.128230826360266	34.0	31.0	34.0	30.0	34.0
3	32.261969084377434	34.0	31.0	34.0	30.0	34.0
4	35.87991589351305	37.0	35.0	37.0	35.0	37.0
5	35.68377469071663	37.0	35.0	37.0	33.0	37.0
6	35.68974246334152	37.0	35.0	37.0	33.0	37.0
7	35.817843517390806	37.0	35.0	37.0	35.0	37.0
8	35.96790327403265	37.0	35.0	37.0	35.0	37.0
9	37.57666430796723	39.0	37.0	39.0	35.0	39.0
10	37.11442585836054	39.0	37.0	39.0	33.0	39.0
11	37.05727343113606	39.0	37.0	39.0	33.0	39.0
12	37.01227926650586	39.0	37.0	39.0	33.0	39.0
13	36.9887694340612	39.0	37.0	39.0	33.0	39.0
14	38.20756905772149	40.0	38.0	41.0	33.0	41.0
15	38.19986163465762	40.0	37.0	41.0	33.0	41.0
16	38.125202221210934	40.0	37.0	41.0	33.0	41.0
17	38.13911504052316	40.0	37.0	41.0	33.0	41.0
18	38.09511170502403	40.0	37.0	41.0	33.0	41.0
19	38.10452545860921	40.0	37.0	41.0	33.0	41.0
20	38.032244035077106	40.0	37.0	41.0	33.0	41.0
21	37.968174217573804	40.0	37.0	41.0	33.0	41.0
22	37.958260665223385	40.0	37.0	41.0	33.0	41.0
23	37.86200643775883	40.0	37.0	41.0	33.0	41.0
24	37.764136194286685	40.0	37.0	41.0	33.0	41.0
25	37.7488800561879	40.0	37.0	41.0	33.0	41.0
26	37.71096374354536	40.0	37.0	41.0	33.0	41.0
27	37.64830739202374	40.0	37.0	41.0	33.0	41.0
28	37.59568910412386	40.0	36.0	41.0	32.0	41.0
29	37.44548116153233	40.0	36.0	41.0	32.0	41.0
30	37.42466235086049	39.0	36.0	41.0	32.0	41.0
31	37.38683372221185	39.0	36.0	41.0	32.0	41.0
32	37.31923199343422	39.0	36.0	41.0	32.0	41.0
33	37.28249941470934	39.0	36.0	41.0	32.0	41.0
34	37.180521176122944	39.0	36.0	41.0	31.0	41.0
35	37.10629053740643	39.0	35.0	41.0	31.0	41.0
36	37.017802481281656	39.0	35.0	41.0	31.0	41.0
37	36.9342826002513	39.0	35.0	41.0	31.0	41.0
38	36.89256430963323	39.0	35.0	41.0	31.0	41.0
39	36.81277362886347	39.0	35.0	41.0	31.0	41.0
40	36.72721158761516	39.0	35.0	40.0	31.0	41.0
41	36.65475391925532	39.0	35.0	40.0	30.0	41.0
42	36.53859893253506	39.0	35.0	40.0	30.0	41.0
43	36.429597206037215	39.0	35.0	40.0	30.0	41.0
44	36.26349040167161	39.0	35.0	40.0	30.0	41.0
45	36.06056859562685	38.0	35.0	40.0	29.0	41.0
46	35.91921322905953	38.0	35.0	40.0	28.0	41.0
47	35.83993111545439	38.0	35.0	40.0	28.0	41.0
48	35.75604471532953	38.0	34.0	40.0	28.0	41.0
49	35.56429034274797	38.0	34.0	40.0	28.0	41.0
50	35.40442313138833	38.0	34.0	40.0	27.0	41.0
51	34.12670950906609	36.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	6.0
11	11.0
12	12.0
13	19.0
14	26.0
15	42.0
16	69.0
17	145.0
18	285.0
19	507.0
20	850.0
21	1397.0
22	2109.0
23	3130.0
24	4187.0
25	5490.0
26	7090.0
27	9261.0
28	12213.0
29	15957.0
30	20643.0
31	26432.0
32	33846.0
33	45122.0
34	67728.0
35	83824.0
36	102981.0
37	151388.0
38	239381.0
39	306197.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.050523517285583	31.488286733674776	21.23022397122562	27.23096577781402
2	23.18434945929665	28.806208728240122	20.995230867571742	27.014210944891488
3	20.49245084654512	30.75849285243924	20.935781119707066	27.813275181308576
4	20.302702683744002	31.40489925547521	19.97415075859807	28.31824730218272
5	19.203320768217008	33.62102451732154	17.212455686701585	29.963199027759874
6	20.89877847428097	36.53914783433688	18.74990683575648	23.812166855625673
7	87.3102847186966	3.648092566238681	4.6303286659143374	4.411294049150386
8	88.79538852339277	3.3514576148726083	3.8745803224841926	3.9785735392504247
9	80.64121551059705	7.767661967681433	7.063647180652702	4.527475341068815
10	35.10753126592013	38.637776544356264	12.213065090459192	14.04162709926442
11	24.267334467964215	27.66859659137242	29.6909402266982	18.373128713965166
12	27.11311849001148	23.005123375763617	29.630701322888413	20.251056811336486
13	25.200116795079875	23.582785527581436	28.861712696379264	22.35538498095942
14	20.62397683739617	26.51423681166969	29.121958790276548	23.739827560657595
15	20.225365401123582	29.035151636314854	29.418243005667016	21.32123995689455
16	22.428864167848207	27.793721650668722	28.425660194711078	21.351753986771993
17	21.97387192349747	27.707879020639936	28.530705619404117	21.787543436458478
18	22.94549825991114	27.375118263786774	26.791932020353208	22.887451455948877
19	22.897798167229162	30.268427010528214	25.767607603605214	21.066167218637407
20	25.462204252849073	27.889998675971693	25.475006115958575	21.17279095522066
21	24.702597813687298	28.91107878494536	25.21835506581122	21.16796833555612
22	23.00924452347695	26.345532807404737	26.136669533933265	24.508553135185043
23	22.70533180061712	28.539474018794188	25.196171015354345	23.55902316523435
24	21.362013014058377	27.119344053578427	27.462802257687475	24.05584067467572
25	23.409346587645853	28.327191069560588	24.881999265208133	23.38146307758543
26	23.841102573612904	28.688186072449778	24.724694180150273	22.746017173787045
27	22.574770333698975	27.80231468207099	24.905586259567418	24.717328724662615
28	23.87021365958794	28.034151161944443	26.020137506039237	22.075497672428384
29	23.80559055608312	27.210272355253455	24.918475806670823	24.0656612819926
30	25.101823037917185	26.744845715628536	25.115151004990093	23.038180241464183
31	24.43305721643654	27.3283826950377	23.29930317530047	24.93925691322529
32	24.32161086018875	27.45122797049258	24.967666527249115	23.25949464206955
33	24.031464524371327	27.14521083177913	25.231156928920722	23.592167714928813
34	23.183384935363744	27.025259128122975	26.628839791697906	23.162516144815378
35	23.478178522857903	25.809345184701947	26.671717264715344	24.0407590277248
36	22.186505608706668	29.017965573510313	27.024294604190068	21.771234213592948
37	23.27580386493508	28.072819803254657	26.148419189115963	22.502957142694303
38	23.36883658246373	25.77444695512947	28.922740756134154	21.933975706272648
39	22.662629695587476	24.604391740518512	28.585683483579853	24.147295080314155
40	23.68791863626838	24.66445527634049	29.136163597288462	22.51146249010267
41	21.155692576410022	25.448262497818863	29.229722418780508	24.166322506990607
42	23.30447653094061	26.4787247941399	26.154469384695112	24.062329290224373
43	22.219737842395034	26.164991463963194	27.31470399198919	24.300566701652578
44	24.378605456224204	25.565145261688492	25.921580696894846	24.134668585192454
45	24.137123737021675	24.597113969024754	27.175023389705373	24.0907389042482
46	21.228470291347605	25.973752673265764	28.285979592427257	24.51179744295937
47	22.179753941176315	26.219092488199923	27.203169951747498	24.39798361887626
48	22.71164504817797	24.31854192040222	28.842685269702812	24.12712776171699
49	22.896307539332845	24.85578175103182	27.138283796260982	25.10962691337435
50	21.317907965126324	24.655862244938223	27.803980677955103	26.22224911198035
51	21.289322983114694	23.93045256339772	29.240244498048597	25.539979955438994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	966.0
1	815.5
2	665.0
3	528.5
4	392.0
5	424.0
6	456.0
7	505.0
8	554.0
9	651.5
10	749.0
11	847.5
12	946.0
13	1148.0
14	1350.0
15	1398.0
16	1446.0
17	1641.0
18	1836.0
19	2173.5
20	2511.0
21	2657.0
22	2803.0
23	3384.5
24	3966.0
25	4814.5
26	7147.5
27	8632.0
28	11702.0
29	14772.0
30	16651.0
31	18530.0
32	22521.0
33	26512.0
34	30705.5
35	34899.0
36	37166.0
37	39433.0
38	41249.5
39	43066.0
40	46978.0
41	50890.0
42	54534.0
43	58178.0
44	66208.5
45	74239.0
46	83793.5
47	93348.0
48	102163.5
49	110979.0
50	107280.0
51	103581.0
52	94230.5
53	84880.0
54	77082.0
55	69284.0
56	64349.5
57	59415.0
58	56212.5
59	53010.0
60	50775.5
61	48541.0
62	44329.0
63	40117.0
64	33525.0
65	26933.0
66	22603.5
67	18274.0
68	15630.0
69	12986.0
70	11097.5
71	9209.0
72	7739.0
73	6269.0
74	5138.0
75	3570.0
76	3133.0
77	2317.0
78	1501.0
79	1124.5
80	748.0
81	579.5
82	411.0
83	296.0
84	181.0
85	133.0
86	85.0
87	56.0
88	27.0
89	47.0
90	67.0
91	39.5
92	12.0
93	8.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1140459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.878169681910077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47742044126203	22.25249005414985
2	9.539278668567452	5.700323734049672
3	3.3465076864521057	2.99962573492897
4	1.7934433216137202	2.1433921551225263
5	1.1410151524195413	1.7045722166810773
6	0.8225272229046378	1.4745364760361026
7	0.6741115534208795	1.409885356435152
8	0.5559819618727104	1.3289378717531284
9	0.4995760489153316	1.3433776162659439
>10	6.4372017793788014	44.26373164576501
>50	0.6637176560115867	12.401465491662492
>100	0.04745017758201185	2.322162214934367
>500	0.001178886399445635	0.24435933965593318
>1k	5.894431997228175E-4	0.4111400925598386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAGTTTCTCGTATGCCGTCTTCTGCT	3594	0.3151362740791208	Illumina PCR Primer Index 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7536798780140277E-4	0.0	0.0	0.387300201059398	0.0
2	1.7536798780140277E-4	0.0	0.0	0.5973033664515778	0.0
3	1.7536798780140277E-4	0.0	0.0	0.7885421571490075	0.0
4	1.7536798780140277E-4	0.0	0.0	1.5521820600302159	0.0
5	1.7536798780140277E-4	0.0	0.0	1.7446484266422555	0.0
6	1.7536798780140277E-4	0.0	0.0	2.9981788034466823	0.0
7	1.7536798780140277E-4	0.0	0.0	4.021801748243471	0.0
8	1.7536798780140277E-4	0.0	0.0	5.154854317428334	0.0
9	1.7536798780140277E-4	0.0	0.0	6.261864740424689	0.0
10	1.7536798780140277E-4	0.0	0.0	7.972754829415174	0.0
11	1.7536798780140277E-4	0.0	0.0	9.265918371462718	0.0
12	1.7536798780140277E-4	0.0	0.0	9.907940574803654	0.0
13	1.7536798780140277E-4	0.0	0.0	10.254467718699226	0.0
14	1.7536798780140277E-4	0.0	0.0	10.571796092625863	0.0
15	1.7536798780140277E-4	0.0	0.0	10.833708182407259	0.0
16	1.7536798780140277E-4	0.0	0.0	11.282124127215445	0.0
17	1.7536798780140277E-4	0.0	0.0	11.860049331014968	0.0
18	1.7536798780140277E-4	0.0	0.0	12.514522661489803	0.0
19	1.7536798780140277E-4	0.0	0.0	12.890160891360408	0.0
20	1.7536798780140277E-4	0.0	0.0	13.29543631116945	0.0
21	1.7536798780140277E-4	0.0	0.0	13.757355591038346	0.0
22	1.7536798780140277E-4	0.0	0.0	14.273814315113476	0.0
23	1.7536798780140277E-4	0.0	0.0	14.776243600164495	0.0
24	1.7536798780140277E-4	0.0	0.0	15.154512349852121	0.0
25	1.7536798780140277E-4	0.0	0.0	15.491744990394219	0.0
26	1.7536798780140277E-4	0.0	0.0	15.807933472400148	0.0
27	1.7536798780140277E-4	0.0	0.0	16.168577739313733	0.0
28	1.7536798780140277E-4	0.0	0.0	16.531238738087033	0.0
29	1.7536798780140277E-4	0.0	0.0	16.919240411097636	0.0
30	1.7536798780140277E-4	0.0	0.0	17.446484266422555	0.0
31	1.7536798780140277E-4	0.0	0.0	17.83474899141486	0.0
32	1.7536798780140277E-4	0.0	0.0	18.235201791559362	0.0
33	1.7536798780140277E-4	0.0	0.0	18.616802533015214	0.0
34	1.7536798780140277E-4	0.0	0.0	18.98568909535547	0.0
35	1.7536798780140277E-4	0.0	0.0	19.3899123072377	0.0
36	1.7536798780140277E-4	0.0	0.0	19.775195776437382	0.0
37	1.7536798780140277E-4	0.0	0.0	20.14899264243607	0.0
38	1.7536798780140277E-4	0.0	0.0	20.54392135096483	0.0
39	1.7536798780140277E-4	0.0	0.0	20.940253003396002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACA	20	7.0332625E-4	45.000004	29
CGTAGCG	45	3.8562575E-10	45.0	1
ACGGGTA	190	0.0	41.44737	5
GTAACGG	245	0.0	41.32653	2
TAAGACG	50	1.0822987E-9	40.5	1
ACGAATA	100	0.0	40.5	12
TCTAGCG	85	0.0	39.705883	1
AATCGTT	165	0.0	39.545452	22
CTTAACG	40	3.45819E-7	39.375004	1
ACGCATG	80	0.0	39.375004	1
CGTAACG	40	3.45819E-7	39.375004	1
CGAATAG	75	0.0	39.0	1
TAGCACG	105	0.0	38.57143	1
CCATCGC	35	6.248247E-6	38.571426	26
ATAAGCG	70	0.0	38.571426	1
AATTACG	70	0.0	38.571426	1
ATACCCG	70	0.0	38.571426	1
TCGAACA	35	6.248247E-6	38.571426	13
GTACGAG	35	6.248247E-6	38.571426	1
ATCGTTG	170	0.0	38.382355	23
>>END_MODULE