SRR3553548_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589820
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	6791	1.1513682140313994	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1735	0.29415753958834895	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1426	0.24176867518904072	Illumina Single End Adapter 2 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1191	0.20192601132548912	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	825	0.13987318164863857	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	646	0.10952493981214609	No Hit
AACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	626	0.10613407480248212	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	110	0.0	45.000004	21
TGCGACG	30	2.163697E-6	45.000004	1
CCATACG	55	1.8189894E-12	45.000004	1
TTACGCG	30	2.163697E-6	45.000004	1
TACTACG	30	2.163697E-6	45.000004	1
CCGGTTA	30	2.163697E-6	45.000004	35
TATACGG	55	1.8189894E-12	45.000004	2
CCCGGTT	30	2.163697E-6	45.000004	34
TATCGCG	30	2.163697E-6	45.000004	1
CTTAACG	25	3.888489E-5	45.0	1
CTTCGGT	20	7.03049E-4	45.0	32
CGGGTCC	20	7.03049E-4	45.0	6
AAGTACG	35	1.210592E-7	45.0	1
CGGCGCA	25	3.888489E-5	45.0	11
ATCTCGC	25	3.888489E-5	45.0	43
GTACACG	25	3.888489E-5	45.0	1
GATCGAT	25	3.888489E-5	45.0	9
CACGGAT	20	7.03049E-4	45.0	18
TAAAGCG	20	7.03049E-4	45.0	1
CGAAACG	25	3.888489E-5	45.0	1