##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589820
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864156861415346	33.0	31.0	34.0	30.0	34.0
2	32.07279678545997	34.0	31.0	34.0	30.0	34.0
3	32.20964531551999	34.0	31.0	34.0	30.0	34.0
4	35.8506323963243	37.0	35.0	37.0	35.0	37.0
5	35.651966701705604	37.0	35.0	37.0	33.0	37.0
6	35.654406429080055	37.0	35.0	37.0	33.0	37.0
7	35.81998236750195	37.0	35.0	37.0	35.0	37.0
8	36.00319080397409	37.0	35.0	37.0	35.0	37.0
9	37.629804008002445	39.0	37.0	39.0	35.0	39.0
10	37.109872503475636	39.0	37.0	39.0	33.0	39.0
11	37.07503475636635	39.0	37.0	39.0	33.0	39.0
12	37.12964294191448	39.0	37.0	39.0	33.0	39.0
13	37.086516225289074	39.0	37.0	39.0	33.0	39.0
14	38.30081889389984	40.0	38.0	41.0	34.0	41.0
15	38.255064256891934	40.0	38.0	41.0	33.0	41.0
16	38.16520972500085	40.0	37.0	41.0	33.0	41.0
17	38.16389067851209	40.0	37.0	41.0	33.0	41.0
18	38.122064358617884	40.0	37.0	41.0	33.0	41.0
19	38.12664202638093	40.0	37.0	41.0	34.0	41.0
20	38.05840256349395	40.0	37.0	41.0	33.0	41.0
21	38.020706317181514	40.0	37.0	41.0	33.0	41.0
22	38.05318910854159	40.0	37.0	41.0	33.0	41.0
23	37.94300295005256	40.0	37.0	41.0	33.0	41.0
24	37.86047777287986	40.0	37.0	41.0	33.0	41.0
25	37.89352344783154	40.0	37.0	41.0	33.0	41.0
26	37.825200908751825	40.0	37.0	41.0	33.0	41.0
27	37.77829676850565	40.0	37.0	41.0	33.0	41.0
28	37.768356447729815	40.0	37.0	41.0	33.0	41.0
29	37.66523685192092	40.0	37.0	41.0	33.0	41.0
30	37.573749618527685	39.0	37.0	41.0	32.0	41.0
31	37.56825980807704	39.0	37.0	41.0	33.0	41.0
32	37.500935878742666	39.0	37.0	41.0	32.0	41.0
33	37.39678206910583	39.0	36.0	41.0	32.0	41.0
34	37.38167576548778	39.0	36.0	41.0	32.0	41.0
35	37.27997863755044	39.0	36.0	41.0	32.0	41.0
36	37.15538130277034	39.0	36.0	41.0	31.0	41.0
37	37.130505917059445	39.0	36.0	41.0	31.0	41.0
38	37.10044928961378	39.0	36.0	41.0	31.0	41.0
39	37.017878335763456	39.0	36.0	41.0	31.0	41.0
40	36.96801736122885	39.0	35.0	41.0	31.0	41.0
41	36.92008070258723	39.0	35.0	40.0	31.0	41.0
42	36.848202841544875	39.0	35.0	40.0	31.0	41.0
43	36.73087043504798	39.0	35.0	40.0	31.0	41.0
44	36.63808789122105	39.0	35.0	40.0	30.0	41.0
45	36.51704926926859	39.0	35.0	40.0	30.0	41.0
46	36.41422298328303	39.0	35.0	40.0	30.0	41.0
47	36.34185005594927	39.0	35.0	40.0	30.0	41.0
48	36.29748228273032	39.0	35.0	40.0	30.0	41.0
49	36.135780407581976	38.0	35.0	40.0	30.0	41.0
50	35.96427384625818	38.0	35.0	40.0	29.0	41.0
51	34.70392492624869	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	1.0
12	1.0
13	4.0
14	4.0
15	13.0
16	29.0
17	48.0
18	124.0
19	259.0
20	451.0
21	657.0
22	1064.0
23	1437.0
24	1852.0
25	2393.0
26	2993.0
27	3836.0
28	5180.0
29	6861.0
30	9198.0
31	12415.0
32	16537.0
33	22417.0
34	32439.0
35	42486.0
36	54835.0
37	81264.0
38	132056.0
39	158919.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.25319589027161	31.65813298972568	22.994811976535214	27.093859143467498
2	21.516055745820758	30.810925367061138	22.608422908684005	25.064595978434102
3	20.98352039605303	31.541656776643723	21.982130141399068	25.492692685904174
4	19.5641043030077	31.658811162727613	21.607778644332168	27.16930588993252
5	18.187413109084126	34.34997117764742	18.973076531823267	28.48953918144519
6	21.40110542199315	35.051710691397375	19.61411956190024	23.933064324709232
7	85.50761249194669	4.005289749415076	5.693940524227731	4.793157234410498
8	87.22186429758231	3.7618256417212033	4.3177579600556095	4.698552100640874
9	78.63687226611509	9.090570004408125	8.693160625275507	3.5793971042012815
10	27.956325658675528	47.28476484351158	12.004170763961888	12.754738733851006
11	18.74148045166322	26.11576413142993	37.32443796412465	17.818317452782203
12	22.59265538638907	24.907598928486657	33.787257129293685	18.712488555830593
13	23.10315011359398	26.54318266589807	30.682411583194874	19.671255637313077
14	18.923739445932657	29.07819334712285	32.01451290224136	19.98355430470313
15	18.45953002610966	31.00590010511681	29.803499372689974	20.73107049608355
16	21.45993692991082	31.194093113153166	27.394628869824693	19.95134108711132
17	20.947238140449628	29.601064731613036	29.78095012037571	19.67074700756163
18	21.420941982299684	27.67657929537825	28.73147739988471	22.171001322437352
19	21.24105659353701	30.506595232443797	28.73113831338374	19.521209860635448
20	22.562815774304024	29.822149130243126	28.149435420975895	19.46559967447696
21	20.930453358651793	30.988776236818012	27.154725170390964	20.926045234139227
22	21.14509511376352	27.276626767488388	29.045471499779595	22.532806618968497
23	21.619138042114542	28.77844088026856	27.276626767488388	22.325794310128515
24	18.208606015394526	30.414533247431418	28.073480044759417	23.303380692414635
25	19.96083550913838	29.69736529788749	27.300362822556036	23.041436370418094
26	22.702349869451695	30.177850869756874	26.447560272625548	20.67223898816588
27	19.34471533688244	28.56176460615103	28.097046556576583	23.99647350038995
28	21.21342104370825	28.419009189244175	29.696687124885557	20.670882642162017
29	23.52853412905632	27.347495846190363	28.21843952392255	20.905530500830764
30	21.3380353328134	27.888847444983217	29.48696212403784	21.286155098165544
31	25.93248787765759	28.16215116476213	24.324370146824457	21.580990810755825
32	24.084296904140245	26.70475738360856	28.35458275406056	20.856362958190637
33	22.61181377369367	27.69760265843817	28.150622223729272	21.539961344138888
34	23.578379844698382	25.758706045912312	29.836729849784682	20.826184259604624
35	24.058695873317284	27.302058255060867	29.012241022684886	19.627004848936963
36	23.60957580278729	27.797124546471807	30.482689634125666	18.11061001661524
37	24.435251432640467	27.728968159777562	27.140992167101828	20.694788240480147
38	23.51514021226815	27.75389101759859	29.94388118409006	18.7870875860432
39	23.598385948255398	26.665762435997426	27.870875860431997	21.86497575531518
40	24.979654809942016	27.792885965209724	25.669526296158153	21.55793292869011
41	23.589569699230275	25.23227425316198	29.673629242819842	21.5045268047879
42	26.603879149571057	25.278898647044862	26.976196127632157	21.141026075751924
43	22.468549727035366	25.692753721474347	28.851344477976333	22.987352073513954
44	23.797090637821707	24.203824895730904	28.42290868400529	23.576175782442103
45	24.807737953952053	25.5893323386796	28.569054965921808	21.033874741446542
46	21.619816215116476	25.608999355735644	31.191549964395914	21.57963446475196
47	22.782204740429286	25.968770133260993	27.669458478857955	23.579566647451767
48	23.871181038282867	24.36116103217931	31.27276118137737	20.494896748160453
49	24.001559797904445	23.834390152928012	29.469499169238073	22.69455087992947
50	22.44210097995999	23.77759316401614	27.673018887118104	26.10728696890577
51	22.394967956325658	22.558746736292427	31.573022277983114	23.4732630293988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2099.0
1	1693.5
2	1288.0
3	866.0
4	444.0
5	419.5
6	395.0
7	431.0
8	467.0
9	514.0
10	561.0
11	652.5
12	744.0
13	829.0
14	914.0
15	1182.5
16	1451.0
17	1280.0
18	1109.0
19	1182.0
20	1255.0
21	1820.0
22	2385.0
23	2894.5
24	3404.0
25	3522.0
26	4664.5
27	5689.0
28	7207.0
29	8725.0
30	9696.5
31	10668.0
32	12321.0
33	13974.0
34	16147.0
35	18320.0
36	19977.0
37	21634.0
38	23703.5
39	25773.0
40	27889.0
41	30005.0
42	33486.0
43	36967.0
44	42839.5
45	48712.0
46	53607.5
47	58503.0
48	66246.5
49	73990.0
50	68510.5
51	63031.0
52	54713.0
53	46395.0
54	38939.5
55	31484.0
56	26280.5
57	21077.0
58	18806.5
59	16536.0
60	14907.0
61	13278.0
62	11384.5
63	9491.0
64	8043.5
65	6596.0
66	5007.0
67	3418.0
68	2637.5
69	1857.0
70	1533.5
71	1210.0
72	963.5
73	717.0
74	789.5
75	616.5
76	371.0
77	270.0
78	169.0
79	144.5
80	120.0
81	75.0
82	30.0
83	32.0
84	34.0
85	20.5
86	7.0
87	10.0
88	13.0
89	8.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	589820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.73997211974021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1390073321874	21.454120668049878
2	11.916233491137707	7.087769035974999
3	4.607243634850373	4.110578917479019
4	2.26426945244218	2.6935724154883944
5	1.3178566724470346	1.9596510348194207
6	0.8656380200903181	1.5446430349963907
7	0.6089042898759397	1.2676157623150461
8	0.4602590669906581	1.0950473456127852
9	0.38707464393546676	1.0360430207009235
>10	4.37072531561499	30.686984482856133
>50	0.938023513097119	18.819186855959142
>100	0.11961373656943115	5.807945527018851
>500	0.002861572645201702	0.5402344196805038
>1k	0.0017169435871210214	0.7407373013388634
>5k	5.723145290403404E-4	1.1558701777096096
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	6791	1.1513682140313994	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1735	0.29415753958834895	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1426	0.24176867518904072	Illumina Single End Adapter 2 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1191	0.20192601132548912	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	825	0.13987318164863857	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	646	0.10952493981214609	No Hit
AACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	626	0.10613407480248212	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6954325048319826E-4	0.0	0.0	1.281916516903462	0.0
2	1.6954325048319826E-4	0.0	0.0	1.792411244108372	0.0
3	1.6954325048319826E-4	0.0	0.0	2.2330541521142044	0.0
4	1.6954325048319826E-4	0.0	0.0	4.321318368315757	0.0
5	1.6954325048319826E-4	0.0	0.0	4.623444440676817	0.0
6	1.6954325048319826E-4	0.0	0.0	6.238174358278797	0.0
7	1.6954325048319826E-4	0.0	0.0	7.433115187684378	0.0
8	1.6954325048319826E-4	0.0	0.0	8.395612220677496	0.0
9	1.6954325048319826E-4	0.0	0.0	9.760096300566275	0.0
10	1.6954325048319826E-4	0.0	0.0	11.702892407853243	0.0
11	1.6954325048319826E-4	0.0	0.0	13.43613305754298	0.0
12	1.6954325048319826E-4	0.0	0.0	14.391339730765319	0.0
13	1.6954325048319826E-4	0.0	0.0	14.753823200298395	0.0
14	1.6954325048319826E-4	0.0	0.0	15.156318876945509	0.0
15	1.6954325048319826E-4	0.0	0.0	15.519310976230036	0.0
16	1.6954325048319826E-4	0.0	0.0	16.313282018242855	0.0
17	1.6954325048319826E-4	0.0	0.0	17.440405547455157	0.0
18	1.6954325048319826E-4	0.0	0.0	18.733851005391475	0.0
19	1.6954325048319826E-4	0.0	0.0	19.36031331592689	0.0
20	1.6954325048319826E-4	0.0	0.0	20.02746600657828	0.0
21	1.6954325048319826E-4	0.0	0.0	20.757180156657963	0.0
22	1.6954325048319826E-4	0.0	0.0	21.57336136448408	0.0
23	1.6954325048319826E-4	0.0	0.0	22.383099928791836	0.0
24	1.6954325048319826E-4	0.0	0.0	22.95429113966973	0.0
25	1.6954325048319826E-4	0.0	0.0	23.463090434369807	0.0
26	1.6954325048319826E-4	0.0	0.0	23.981384151096943	0.0
27	1.6954325048319826E-4	0.0	0.0	24.53663829642942	0.0
28	1.6954325048319826E-4	0.0	0.0	25.11172900206843	0.0
29	1.6954325048319826E-4	0.0	0.0	25.67376487742023	0.0
30	1.6954325048319826E-4	0.0	0.0	26.435183615340275	0.0
31	1.6954325048319826E-4	0.0	0.0	26.99840629344546	0.0
32	1.6954325048319826E-4	0.0	0.0	27.5519650062731	0.0
33	1.6954325048319826E-4	0.0	0.0	28.098911532331897	0.0
34	1.6954325048319826E-4	0.0	0.0	28.62907327659286	0.0
35	1.6954325048319826E-4	0.0	0.0	29.196195449459157	0.0
36	3.390865009663965E-4	0.0	0.0	29.746871927028586	0.0
37	3.390865009663965E-4	0.0	0.0	30.29330982333593	0.0
38	3.390865009663965E-4	0.0	0.0	30.850768030924687	0.0
39	3.390865009663965E-4	0.0	0.0	31.41907700654437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	110	0.0	45.000004	21
TGCGACG	30	2.163697E-6	45.000004	1
CCATACG	55	1.8189894E-12	45.000004	1
TTACGCG	30	2.163697E-6	45.000004	1
TACTACG	30	2.163697E-6	45.000004	1
CCGGTTA	30	2.163697E-6	45.000004	35
TATACGG	55	1.8189894E-12	45.000004	2
CCCGGTT	30	2.163697E-6	45.000004	34
TATCGCG	30	2.163697E-6	45.000004	1
CTTAACG	25	3.888489E-5	45.0	1
CTTCGGT	20	7.03049E-4	45.0	32
CGGGTCC	20	7.03049E-4	45.0	6
AAGTACG	35	1.210592E-7	45.0	1
CGGCGCA	25	3.888489E-5	45.0	11
ATCTCGC	25	3.888489E-5	45.0	43
GTACACG	25	3.888489E-5	45.0	1
GATCGAT	25	3.888489E-5	45.0	9
CACGGAT	20	7.03049E-4	45.0	18
TAAAGCG	20	7.03049E-4	45.0	1
CGAAACG	25	3.888489E-5	45.0	1
>>END_MODULE