##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594643
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.853794629719008	33.0	31.0	34.0	30.0	34.0
2	32.051267735431175	33.0	31.0	34.0	30.0	34.0
3	32.19062866291203	34.0	31.0	34.0	30.0	34.0
4	35.80582635295463	37.0	35.0	37.0	35.0	37.0
5	35.63365750542763	37.0	35.0	37.0	33.0	37.0
6	35.64409738279943	37.0	35.0	37.0	33.0	37.0
7	35.82900496600481	37.0	35.0	37.0	35.0	37.0
8	36.01879110659673	37.0	35.0	37.0	35.0	37.0
9	37.62868645557082	39.0	37.0	39.0	35.0	39.0
10	37.10200237789733	39.0	37.0	39.0	33.0	39.0
11	37.08161871913064	39.0	37.0	39.0	33.0	39.0
12	37.13864284957529	39.0	37.0	39.0	33.0	39.0
13	37.08141187233348	39.0	37.0	39.0	33.0	39.0
14	38.293653503026185	40.0	38.0	41.0	34.0	41.0
15	38.246193094007666	40.0	38.0	41.0	33.0	41.0
16	38.19258445823797	40.0	37.0	41.0	33.0	41.0
17	38.16144812938183	40.0	37.0	41.0	33.0	41.0
18	38.097927664161524	40.0	37.0	41.0	33.0	41.0
19	38.09383613361294	40.0	37.0	41.0	33.0	41.0
20	38.026145098824	40.0	37.0	41.0	33.0	41.0
21	38.00393849755231	40.0	37.0	41.0	33.0	41.0
22	38.03271542757587	40.0	37.0	41.0	33.0	41.0
23	37.92938115810663	40.0	37.0	41.0	33.0	41.0
24	37.8717146254139	40.0	37.0	41.0	33.0	41.0
25	37.90720650877922	40.0	37.0	41.0	33.0	41.0
26	37.83103643698824	40.0	37.0	41.0	33.0	41.0
27	37.77420233652797	40.0	37.0	41.0	33.0	41.0
28	37.75132642610777	40.0	37.0	41.0	33.0	41.0
29	37.64760705162593	40.0	37.0	41.0	33.0	41.0
30	37.56426460918568	39.0	37.0	41.0	32.0	41.0
31	37.55392395100926	39.0	37.0	41.0	33.0	41.0
32	37.502728527873025	39.0	37.0	41.0	32.0	41.0
33	37.38769312007373	39.0	36.0	41.0	32.0	41.0
34	37.3422742721263	39.0	36.0	41.0	32.0	41.0
35	37.24340991149312	39.0	36.0	41.0	31.0	41.0
36	37.155032515307504	39.0	36.0	41.0	31.0	41.0
37	37.094752649909275	39.0	36.0	41.0	31.0	41.0
38	37.03218737965469	39.0	36.0	40.0	31.0	41.0
39	36.967176944822356	39.0	35.0	40.0	31.0	41.0
40	36.94053743170272	39.0	35.0	40.0	31.0	41.0
41	36.92533671463382	39.0	35.0	40.0	31.0	41.0
42	36.841165203323676	39.0	35.0	40.0	31.0	41.0
43	36.723191898332274	39.0	35.0	40.0	31.0	41.0
44	36.68366229821927	39.0	35.0	40.0	31.0	41.0
45	36.56334809288935	39.0	35.0	40.0	30.0	41.0
46	36.438286501312554	39.0	35.0	40.0	30.0	41.0
47	36.34138129936785	39.0	35.0	40.0	30.0	41.0
48	36.32322586829409	39.0	35.0	40.0	30.0	41.0
49	36.16455083133914	38.0	35.0	40.0	30.0	41.0
50	35.95419100199616	38.0	35.0	40.0	29.0	41.0
51	34.74337880038948	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	1.0
13	3.0
14	3.0
15	6.0
16	17.0
17	41.0
18	113.0
19	276.0
20	437.0
21	698.0
22	1054.0
23	1404.0
24	1963.0
25	2393.0
26	3086.0
27	3866.0
28	5136.0
29	6871.0
30	9249.0
31	12548.0
32	16328.0
33	22622.0
34	33483.0
35	43004.0
36	55607.0
37	82328.0
38	133992.0
39	158073.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.385148736300604	31.581806226593102	22.941664158158762	27.091380878947536
2	21.4927275693147	30.818995599040093	22.59086544363593	25.097411388009277
3	20.933904880743572	31.705746136757686	21.911298039327797	25.449050943170942
4	19.67382109938232	31.56566208632743	21.64357437992207	27.116942434368184
5	18.2576772954529	34.250802582389774	18.929340797755962	28.562179324401367
6	21.35331619139551	35.17303659506628	19.57863793906596	23.895009274472244
7	85.58815961846015	3.965068116500152	5.656166809329295	4.790605455710401
8	87.34568472175742	3.7341732770754894	4.290137107474569	4.630004893692518
9	78.80745253875014	9.00775759573391	8.638964891539965	3.545824973975982
10	28.009410688429863	47.31578442864038	11.986855979133699	12.687948903796059
11	18.750914414194735	26.116173906024287	37.35451354846521	17.778398131315765
12	22.605159734496162	24.952954966257064	33.70341532650683	18.738469972739946
13	23.137243690752264	26.499092732950697	30.669830469710398	19.69383310658664
14	18.92950896588373	29.11124826156198	31.91629263272249	20.0429501398318
15	18.34495655376419	31.03492347509346	29.888353179975212	20.731766791167136
16	21.438072927790287	31.18358410003986	27.481362767240174	19.896980204929683
17	21.03766461557607	29.579428329266467	29.726743609190727	19.65616344596674
18	21.483814658543025	27.50591531389422	28.738419522301616	22.27185050526114
19	21.3208597427364	30.370491202284395	28.71403514377534	19.594613911203865
20	22.671249808708755	29.767103959854907	28.12679204161152	19.43485418982482
21	21.04994088890309	30.992713275023835	27.143008494172133	20.814337341900938
22	21.18733424928907	27.214984452856587	29.097794811340588	22.499886486513756
23	21.597832649169334	28.856305379866576	27.333711151060385	22.212150819903705
24	18.227238864327	30.39739810272718	27.93423953531783	23.441123497627988
25	20.003935134189756	29.83907991853936	27.293350800396205	22.863634146874677
26	22.789808338784784	30.026419212872263	26.532726358504178	20.651046089838776
27	19.373977327573012	28.56049764312369	28.01613741354056	24.049387615762736
28	21.106949884216245	28.599680816893496	29.729434299235002	20.563934999655256
29	23.55228263008225	27.391729155140144	28.247200421092995	20.808787793684612
30	21.368114986639043	27.87252183242719	29.47381874502853	21.28554443590524
31	25.886456243493996	28.196918150890532	24.425916053833983	21.49070955178149
32	24.023489724086552	26.917663202963794	28.268557773319454	20.7902892996302
33	22.52679338695654	27.783897229093757	27.971741027809966	21.717568356139736
34	23.515285641973417	25.825579381242193	29.890875701891723	20.768259274892667
35	23.964967215623492	27.385506934412753	28.918023082757216	19.731502767206543
36	23.44095532950022	27.835524844318353	30.48013682158875	18.243383004592673
37	24.585675775213026	27.659116478290336	27.058419925905124	20.696787820591513
38	23.528570924067047	27.768930265722457	29.936785600772225	18.765713209438267
39	23.60760994411773	26.71569328151513	27.774647982066554	21.90204879230059
40	24.994492493815617	27.749590931029207	25.840714512741258	21.41520206241392
41	23.708847157033716	25.177627584954333	29.516533449481454	21.596991808530497
42	26.481435079535114	25.388678585302443	27.010155673235875	21.119730661926567
43	22.534529120833845	25.681459295745512	28.66392104170065	23.12009054171999
44	23.715573882144415	24.3927869326638	28.365590783041256	23.526048402150536
45	24.812198243315738	25.537675546504374	28.650299423351488	20.9998267868284
46	21.791562332357397	25.591657515517714	31.12018471587154	21.496595436253347
47	22.73380835223823	25.993243004626304	27.711753102281538	23.561195540853923
48	23.921916174915033	24.33930946803376	31.291548038066537	20.44722631898467
49	24.176186384099367	23.786877168317798	29.37089985083487	22.666036596747965
50	22.31473337784183	23.893832097577874	27.714948296709117	26.076486227871175
51	22.448763375672463	22.63458915685546	31.513866302975064	23.402781164497018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2076.0
1	1674.5
2	1273.0
3	894.5
4	516.0
5	447.5
6	379.0
7	440.5
8	502.0
9	529.5
10	557.0
11	632.0
12	707.0
13	795.0
14	883.0
15	1146.5
16	1410.0
17	1264.5
18	1119.0
19	1206.0
20	1293.0
21	1807.0
22	2321.0
23	2893.0
24	3465.0
25	3567.5
26	4781.5
27	5893.0
28	7414.0
29	8935.0
30	9934.5
31	10934.0
32	12494.0
33	14054.0
34	16261.0
35	18468.0
36	20054.0
37	21640.0
38	23706.5
39	25773.0
40	27965.5
41	30158.0
42	33625.5
43	37093.0
44	42947.0
45	48801.0
46	53813.5
47	58826.0
48	66913.5
49	75001.0
50	69343.5
51	63686.0
52	55255.0
53	46824.0
54	39278.5
55	31733.0
56	26566.5
57	21400.0
58	19145.0
59	16890.0
60	15102.0
61	13314.0
62	11413.5
63	9513.0
64	8075.0
65	6637.0
66	5017.0
67	3397.0
68	2696.0
69	1995.0
70	1628.5
71	1262.0
72	988.5
73	715.0
74	754.0
75	586.0
76	379.0
77	274.5
78	170.0
79	137.0
80	104.0
81	63.0
82	22.0
83	28.0
84	34.0
85	19.5
86	5.0
87	11.5
88	18.0
89	10.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	594643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.448001837523304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0763320850435	21.22503959682301
2	11.907482680848721	7.013031437318201
3	4.6047244765188875	4.067998045374503
4	2.284549645107936	2.6910168858820684
5	1.3092367976734376	1.9277203811820256
6	0.848841956818229	1.499801970249005
7	0.6254813995177873	1.289342418163551
8	0.49490725749604436	1.1659223862517707
9	0.3823067276082496	1.0132352295394729
>10	4.371862012600651	30.380720835463208
>50	0.9479508312461418	18.861893605862782
>100	0.14058592836277517	6.366605138619207
>500	0.0034429206945985756	0.5990289889429914
>1k	0.0017214603472992878	0.7328600070650928
>5k	5.738201157664293E-4	1.1657830732631025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	6899	1.1601919134674081	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1693	0.28470864031023657	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1467	0.2467026434348004	Illumina Single End Adapter 2 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1177	0.1979338863822495	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTT	761	0.12797594523100414	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	619	0.10409607108803097	No Hit
AACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	618	0.10392790296026357	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2689966921329268	0.0
2	0.0	0.0	0.0	1.785609180634431	0.0
3	0.0	0.0	0.0	2.241512975011898	0.0
4	0.0	0.0	0.0	4.283914886747175	0.0
5	0.0	0.0	0.0	4.581236136639967	0.0
6	0.0	0.0	0.0	6.190436951246378	0.0
7	0.0	0.0	0.0	7.3882985253336875	0.0
8	0.0	0.0	0.0	8.355937932507404	0.0
9	0.0	0.0	0.0	9.689847521958553	0.0
10	0.0	0.0	0.0	11.648838042321191	0.0
11	0.0	0.0	0.0	13.337918717617125	0.0
12	0.0	0.0	0.0	14.298158727169074	0.0
13	0.0	0.0	0.0	14.652825308630556	0.0
14	0.0	0.0	0.0	15.043311701306498	0.0
15	0.0	0.0	0.0	15.406386689156351	0.0
16	0.0	0.0	0.0	16.198458570940883	0.0
17	0.0	0.0	0.0	17.29743728590095	0.0
18	0.0	0.0	0.0	18.620247778919452	0.0
19	0.0	0.0	0.0	19.268704079590613	0.0
20	0.0	0.0	0.0	19.929941157972095	0.0
21	0.0	0.0	0.0	20.656931974310638	0.0
22	0.0	0.0	0.0	21.498108949403257	0.0
23	0.0	0.0	0.0	22.297075724426254	0.0
24	0.0	0.0	0.0	22.87136988075198	0.0
25	0.0	0.0	0.0	23.344426824161722	0.0
26	0.0	0.0	0.0	23.855321596319136	0.0
27	0.0	0.0	0.0	24.409603745440542	0.0
28	0.0	0.0	0.0	24.976330336016737	0.0
29	0.0	0.0	0.0	25.54759746604265	0.0
30	0.0	0.0	0.0	26.320498181261698	0.0
31	0.0	0.0	0.0	26.898155700142773	0.0
32	0.0	0.0	0.0	27.42384926754372	0.0
33	0.0	0.0	0.0	27.975608894748614	0.0
34	0.0	0.0	0.0	28.490707870100213	0.0
35	0.0	0.0	0.0	29.046335364243756	0.0
36	0.0	0.0	0.0	29.60667156596479	0.0
37	0.0	0.0	0.0	30.151031795547915	0.0
38	0.0	0.0	0.0	30.721626253062762	0.0
39	0.0	0.0	0.0	31.255223722468774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	25	3.8885257E-5	45.000004	6
TTCTACG	95	0.0	45.000004	1
CTCGTAA	25	3.8885257E-5	45.000004	31
CGTATAA	25	3.8885257E-5	45.000004	36
TACCACG	25	3.8885257E-5	45.000004	1
CGTGAAC	25	3.8885257E-5	45.000004	34
CGCGCCG	25	3.8885257E-5	45.000004	19
CACGACC	105	0.0	45.000004	27
CCGTCGG	25	3.8885257E-5	45.000004	1
AGCGGAT	55	1.8189894E-12	45.000004	4
GCCGAAA	25	3.8885257E-5	45.000004	44
CGAATCC	25	3.8885257E-5	45.000004	24
CGAATAG	25	3.8885257E-5	45.000004	24
TCATCGT	25	3.8885257E-5	45.000004	38
CATCGCG	25	3.8885257E-5	45.000004	1
CGTAAAC	25	3.8885257E-5	45.000004	20
TCATACG	25	3.8885257E-5	45.000004	1
GCGTATA	25	3.8885257E-5	45.000004	35
ACGAATC	25	3.8885257E-5	45.000004	23
ACGCGTA	25	3.8885257E-5	45.000004	33
>>END_MODULE