FastQCFastQC Report
Sat 18 Jun 2016
SRR3553546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984770
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG453.8380676E-1045.0000041
CTTACCG453.8380676E-1045.0000041
CCTTACG253.890397E-545.01
CGAACAT207.032795E-445.014
CCGGATA253.890397E-545.05
TACGGTT207.032795E-445.024
CACGAAT207.032795E-445.015
TCGATAG207.032795E-445.01
CGTTATG406.8139343E-945.01
ATCGTAC253.890397E-545.028
CGTTACG207.032795E-445.01
AAACGCG253.890397E-545.01
TACGACC207.032795E-445.018
TATTCGA207.032795E-445.043
CTTGACG207.032795E-445.01
CGTACTA207.032795E-445.034
TCGTACT253.890397E-545.029
CGTGTGA207.032795E-445.039
AATTACG351.2116834E-745.01
ATACGAG253.890397E-545.01