##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	984770
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.963510261279282	33.0	31.0	34.0	30.0	34.0
2	32.1626816413985	34.0	31.0	34.0	30.0	34.0
3	32.30489251297257	34.0	31.0	34.0	30.0	34.0
4	35.915973272946985	37.0	35.0	37.0	35.0	37.0
5	35.732285711384385	37.0	35.0	37.0	35.0	37.0
6	35.73771337469663	37.0	35.0	37.0	33.0	37.0
7	35.867152736171896	37.0	35.0	37.0	35.0	37.0
8	36.02979071255217	37.0	35.0	37.0	35.0	37.0
9	37.634700488438924	39.0	37.0	39.0	35.0	39.0
10	37.18320318449993	39.0	37.0	39.0	34.0	39.0
11	37.12485758095799	39.0	37.0	39.0	34.0	39.0
12	37.08500462036821	39.0	37.0	39.0	33.0	39.0
13	37.079106796510864	39.0	37.0	39.0	33.0	39.0
14	38.281951115488894	40.0	38.0	41.0	34.0	41.0
15	38.25935599175442	40.0	38.0	41.0	33.0	41.0
16	38.18716959289986	40.0	37.0	41.0	33.0	41.0
17	38.202112168323566	40.0	37.0	41.0	33.0	41.0
18	38.17029154015658	40.0	37.0	41.0	33.0	41.0
19	38.173664916681055	40.0	37.0	41.0	34.0	41.0
20	38.118727215491944	40.0	37.0	41.0	33.0	41.0
21	38.063643287265045	40.0	37.0	41.0	33.0	41.0
22	38.05706713242686	40.0	37.0	41.0	33.0	41.0
23	37.96395198878926	40.0	37.0	41.0	33.0	41.0
24	37.91630025285092	40.0	37.0	41.0	33.0	41.0
25	37.920975456197894	40.0	37.0	41.0	33.0	41.0
26	37.86255064634381	40.0	37.0	41.0	33.0	41.0
27	37.84165135006143	40.0	37.0	41.0	33.0	41.0
28	37.79666318023498	40.0	37.0	41.0	33.0	41.0
29	37.67758562913168	40.0	37.0	41.0	33.0	41.0
30	37.6962112980696	40.0	37.0	41.0	33.0	41.0
31	37.621559348883494	40.0	37.0	41.0	33.0	41.0
32	37.59034698457508	40.0	37.0	41.0	33.0	41.0
33	37.514188084527355	40.0	36.0	41.0	33.0	41.0
34	37.468734831483495	40.0	36.0	41.0	32.0	41.0
35	37.306422819541616	39.0	36.0	41.0	32.0	41.0
36	37.164954253277415	39.0	36.0	41.0	31.0	41.0
37	37.09270997288707	39.0	36.0	41.0	31.0	41.0
38	36.82107801821745	39.0	35.0	41.0	31.0	41.0
39	36.64857276318328	39.0	35.0	41.0	30.0	41.0
40	36.22695147090184	39.0	35.0	41.0	29.0	41.0
41	35.935691582806136	39.0	35.0	41.0	27.0	41.0
42	35.639022309777914	39.0	35.0	41.0	25.0	41.0
43	35.3281588594291	39.0	35.0	40.0	23.0	41.0
44	34.85632381165145	39.0	34.0	40.0	21.0	41.0
45	34.232046061516904	38.0	34.0	40.0	15.0	41.0
46	34.059960193750825	38.0	33.0	40.0	15.0	41.0
47	33.884523289702166	38.0	33.0	40.0	14.0	41.0
48	33.74976288879637	38.0	33.0	40.0	13.0	41.0
49	33.59371223737522	38.0	33.0	40.0	13.0	41.0
50	33.31623627852189	38.0	33.0	40.0	13.0	41.0
51	32.12050326472171	36.0	30.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	4.0
11	6.0
12	8.0
13	12.0
14	15.0
15	32.0
16	59.0
17	117.0
18	175.0
19	385.0
20	654.0
21	1102.0
22	1822.0
23	2704.0
24	3700.0
25	5088.0
26	7251.0
27	9920.0
28	13809.0
29	18797.0
30	24641.0
31	31659.0
32	39127.0
33	47369.0
34	62577.0
35	68233.0
36	74398.0
37	109395.0
38	188364.0
39	273245.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.088853234765477	32.28225880154757	21.36417640667364	26.264711557013314
2	22.136133310316115	30.236501924307195	20.945398417904688	26.681966347472
3	20.42761253896849	30.71894960244524	21.378900657006206	27.474537201580063
4	19.910435939356397	30.55698284878703	20.52255856697503	29.010022644881545
5	18.62434883272236	33.01461254912314	18.289346751018005	30.07169186713649
6	21.093859479878553	36.2697888847142	19.068919646211807	23.56743198919545
7	86.96528123318136	3.65648831706896	4.939630573636484	4.438599876113204
8	88.64211947967546	3.264620165114697	3.9085268641408657	4.184733491068981
9	80.53027610508038	7.848533160027214	7.611320409841891	4.009870325050519
10	33.073204910791354	40.68970419488815	12.771510098804798	13.465580795515706
11	23.920306264406918	26.778638666896835	31.927759781471813	17.373295287224426
12	27.43919900078191	22.51256638605969	31.462676564070797	18.585558049087606
13	26.5694527656204	22.79374879413467	28.94218954679773	21.6946088934472
14	19.593712237375225	26.83733257511906	30.57048854047138	22.998466647034334
15	18.927262203357127	29.488205367750847	31.389156858961993	20.195375569930036
16	22.16842511449374	28.126466078373628	30.056358337479814	19.64875046965281
17	21.09913990068747	28.482589843313665	29.387268093057262	21.0310021629416
18	21.77198736760868	28.2393858464413	27.888745595418218	22.0998811905318
19	22.63685936817734	31.594991723955847	27.042761253896845	18.725387653969964
20	25.33566213430547	28.446337723529354	26.82220213857043	19.39579800359475
21	24.503081937914438	28.85161002061395	26.278623434913733	20.36668460655788
22	21.075174913939296	28.245884825898433	27.71946748987073	22.95947277029154
23	22.556231404287296	28.80408623333367	26.44637834215096	22.193304020228073
24	21.113153325141912	27.42894279882612	27.844979030636598	23.612924845395373
25	22.26773764432304	28.508991947358265	25.441067457375834	23.78220295094286
26	22.82624369142033	29.97278552352326	26.25780639134011	20.943164393716298
27	20.25853752652904	28.221716746042226	27.798775348558546	23.72097037887019
28	21.621596921108484	28.619779237791565	28.440143383734274	21.31848045736568
29	22.49012459762178	28.978441666582043	26.619312123643084	21.91212161215309
30	23.767580247164314	25.99205905947582	27.982879250992614	22.25748144236725
31	23.877250525503417	27.391472120393594	26.307056470038688	22.4242208840643
32	23.74920032088711	26.86546097058196	28.73310519207531	20.65223351645562
33	24.735928186276997	26.956345136427796	27.490276917452803	20.8174497598424
34	22.180813794083896	25.76723498888065	30.93920407810961	21.11274713892584
35	22.319932573088135	25.085451425205886	30.51159153914112	22.08302446256486
36	22.12567401525229	28.391807224021854	29.83935335154402	19.64316540918184
37	24.08816271819816	27.332981305279407	27.24362033774384	21.3352356387786
38	24.151121581689125	25.723976156869117	28.75575007362125	21.369152187820507
39	24.411791585852534	24.94552027376951	26.974420423042943	23.668267717335013
40	25.519258303969455	24.158128293916345	28.014561775846136	22.308051626268064
41	21.0838063710308	25.426952486367377	28.955695238482082	24.533545904119745
42	22.022502716370322	26.659118372818018	27.475146480904172	23.84323242990749
43	21.476791535079258	26.1343257816546	28.431004193872685	23.957878489393465
44	22.957848025427257	25.030920925698386	27.282106481716546	24.729124567157815
45	24.209409303695278	24.105730272043218	27.109477339886475	24.575383084375034
46	20.38770474323954	26.10599429308366	28.74366603369315	24.76263492998365
47	22.072362074393006	25.812829391634594	27.9150461529088	24.1997623810636
48	22.714237842338818	23.20511388446033	30.260263817947337	23.82038445525351
49	21.465418321029276	24.331975994394632	28.615920468738892	25.5866852158372
50	20.396640839993093	24.222711902271595	28.57641885922601	26.804228398509295
51	19.905663251317566	23.23110980228886	30.044782030321798	26.81844491607177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1136.0
1	929.0
2	722.0
3	568.5
4	415.0
5	469.5
6	524.0
7	563.0
8	602.0
9	759.0
10	916.0
11	1047.5
12	1179.0
13	1324.5
14	1470.0
15	1536.5
16	1603.0
17	1724.5
18	1846.0
19	2469.0
20	3092.0
21	3067.5
22	3043.0
23	3876.0
24	4709.0
25	5562.5
26	8596.0
27	10776.0
28	12483.5
29	14191.0
30	16174.0
31	18157.0
32	21556.0
33	24955.0
34	27931.0
35	30907.0
36	34588.5
37	38270.0
38	40404.0
39	42538.0
40	45760.0
41	48982.0
42	52461.0
43	55940.0
44	60845.0
45	65750.0
46	72296.5
47	78843.0
48	84750.0
49	90657.0
50	89716.0
51	88775.0
52	83971.0
53	79167.0
54	70579.0
55	61991.0
56	56382.5
57	50774.0
58	46220.5
59	41667.0
60	39589.0
61	37511.0
62	33968.5
63	30426.0
64	25349.5
65	20273.0
66	16076.0
67	11879.0
68	8837.0
69	5795.0
70	4872.5
71	3950.0
72	2952.0
73	1954.0
74	1550.0
75	1019.0
76	892.0
77	647.0
78	402.0
79	339.0
80	276.0
81	224.0
82	172.0
83	111.0
84	50.0
85	35.0
86	20.0
87	11.5
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	984770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.49766031397544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75150342521542	30.27992445281552
2	8.322383943205828	6.241398522096313
3	2.6426487011725754	2.972794299772129
4	1.2093968712437417	1.8139821225073005
5	0.7408772012315055	1.389058081307391
6	0.5387908866301021	1.2122038588272732
7	0.4077908368262149	1.0703841594922836
8	0.35684756558530073	1.0704759038549343
9	0.2824087140568988	0.9530699426470992
>10	4.069052066514134	35.207998541367864
>50	0.6433694987076751	15.644185066950412
>100	0.033847179855235034	1.8292582392653602
>500	0.0010831097553030253	0.31526680909615956
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5135209236674554	0.0
2	0.0	0.0	0.0	0.7774404175594302	0.0
3	0.0	0.0	0.0	1.0387197010469449	0.0
4	0.0	0.0	0.0	2.2582938147993947	0.0
5	0.0	0.0	0.0	2.5055596738324684	0.0
6	0.0	0.0	0.0	3.993419783299654	0.0
7	0.0	0.0	0.0	5.080780283721072	0.0
8	0.0	0.0	0.0	6.0716715578256855	0.0
9	0.0	0.0	0.0	7.305766828802665	0.0
10	0.0	0.0	0.0	9.13685429084964	0.0
11	0.0	0.0	0.0	10.710724331569809	0.0
12	0.0	0.0	0.0	11.489789493993522	0.0
13	0.0	0.0	0.0	11.831595194817064	0.0
14	0.0	0.0	0.0	12.18203235273211	0.0
15	0.0	0.0	0.0	12.47692354559948	0.0
16	0.0	0.0	0.0	13.07594666774983	0.0
17	0.0	0.0	0.0	13.813073103364237	0.0
18	0.0	0.0	0.0	14.719782284188186	0.0
19	0.0	0.0	0.0	15.200199031245875	0.0
20	0.0	0.0	0.0	15.744793200442743	0.0
21	0.0	0.0	0.0	16.293550778354337	0.0
22	0.0	0.0	0.0	16.968124536693846	0.0
23	0.0	0.0	0.0	17.577302314245966	0.0
24	0.0	0.0	0.0	18.023904058815763	0.0
25	0.0	0.0	0.0	18.414655198675835	0.0
26	0.0	0.0	0.0	18.791697553743514	0.0
27	0.0	0.0	0.0	19.216568335753525	0.0
28	0.0	0.0	0.0	19.627628786417134	0.0
29	0.0	0.0	0.0	20.08651766402307	0.0
30	0.0	0.0	0.0	20.732455294129593	0.0
31	0.0	0.0	0.0	21.17479208343065	0.0
32	0.0	0.0	0.0	21.627080435025437	0.0
33	0.0	0.0	0.0	22.05550534642607	0.0
34	0.0	0.0	0.0	22.464027133239235	0.0
35	0.0	0.0	0.0	22.906567015648324	0.0
36	0.0	0.0	0.0	23.363018775957837	0.0
37	0.0	0.0	0.0	23.820181362145476	0.0
38	0.0	0.0	0.0	24.27703930867106	0.0
39	0.0	0.0	0.0	24.748012226205105	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	45	3.8380676E-10	45.000004	1
CTTACCG	45	3.8380676E-10	45.000004	1
CCTTACG	25	3.890397E-5	45.0	1
CGAACAT	20	7.032795E-4	45.0	14
CCGGATA	25	3.890397E-5	45.0	5
TACGGTT	20	7.032795E-4	45.0	24
CACGAAT	20	7.032795E-4	45.0	15
TCGATAG	20	7.032795E-4	45.0	1
CGTTATG	40	6.8139343E-9	45.0	1
ATCGTAC	25	3.890397E-5	45.0	28
CGTTACG	20	7.032795E-4	45.0	1
AAACGCG	25	3.890397E-5	45.0	1
TACGACC	20	7.032795E-4	45.0	18
TATTCGA	20	7.032795E-4	45.0	43
CTTGACG	20	7.032795E-4	45.0	1
CGTACTA	20	7.032795E-4	45.0	34
TCGTACT	25	3.890397E-5	45.0	29
CGTGTGA	20	7.032795E-4	45.0	39
AATTACG	35	1.2116834E-7	45.0	1
ATACGAG	25	3.890397E-5	45.0	1
>>END_MODULE