##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519977
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.906815109129827	33.0	31.0	34.0	30.0	34.0
2	32.11109914476987	34.0	31.0	34.0	30.0	34.0
3	32.237545122188095	34.0	31.0	34.0	30.0	34.0
4	35.84946449554499	37.0	35.0	37.0	35.0	37.0
5	35.64388424872639	37.0	35.0	37.0	33.0	37.0
6	35.63703394573221	37.0	35.0	37.0	33.0	37.0
7	35.59943228258173	37.0	35.0	37.0	35.0	37.0
8	35.81043969252486	37.0	35.0	37.0	35.0	37.0
9	37.44163684162953	39.0	37.0	39.0	35.0	39.0
10	37.063639353279086	39.0	37.0	39.0	33.0	39.0
11	37.0330630008635	39.0	37.0	39.0	33.0	39.0
12	36.8883008286905	39.0	37.0	39.0	33.0	39.0
13	36.858914913544254	39.0	37.0	39.0	33.0	39.0
14	38.03582273831343	40.0	37.0	41.0	33.0	41.0
15	38.04248264827098	40.0	37.0	41.0	33.0	41.0
16	37.963561849851054	40.0	37.0	41.0	33.0	41.0
17	37.972866107539375	40.0	37.0	41.0	33.0	41.0
18	37.93022383682355	40.0	37.0	41.0	33.0	41.0
19	37.91756366146964	40.0	37.0	41.0	33.0	41.0
20	37.869890398998415	40.0	37.0	41.0	33.0	41.0
21	37.83070981985742	40.0	37.0	41.0	33.0	41.0
22	37.8264230917906	40.0	37.0	41.0	33.0	41.0
23	37.71649899899418	40.0	36.0	41.0	33.0	41.0
24	37.60839421743654	39.0	36.0	41.0	32.0	41.0
25	37.62212174769269	39.0	36.0	41.0	32.0	41.0
26	37.557866982578076	39.0	36.0	41.0	32.0	41.0
27	37.46650524927064	39.0	36.0	41.0	32.0	41.0
28	37.39915996284451	39.0	36.0	41.0	32.0	41.0
29	37.26961384830483	39.0	36.0	41.0	32.0	41.0
30	37.2231367925889	39.0	36.0	41.0	31.0	41.0
31	37.17221146319933	39.0	36.0	41.0	31.0	41.0
32	37.11031834100354	39.0	35.0	41.0	31.0	41.0
33	37.07384172761488	39.0	35.0	41.0	31.0	41.0
34	36.99437090486695	39.0	35.0	41.0	31.0	41.0
35	36.90340149660466	39.0	35.0	41.0	31.0	41.0
36	36.80055848624074	39.0	35.0	41.0	31.0	41.0
37	36.68140321591147	39.0	35.0	40.0	30.0	41.0
38	36.67784728939934	39.0	35.0	40.0	31.0	41.0
39	36.639360971735286	39.0	35.0	40.0	31.0	41.0
40	36.502929937285685	39.0	35.0	40.0	30.0	41.0
41	36.4437042407645	39.0	35.0	40.0	30.0	41.0
42	36.34293632218348	38.0	35.0	40.0	30.0	41.0
43	36.23503924212032	38.0	35.0	40.0	30.0	41.0
44	36.08398833025307	38.0	35.0	40.0	30.0	41.0
45	35.919942612846334	38.0	35.0	40.0	29.0	41.0
46	35.7523505847374	38.0	34.0	40.0	29.0	41.0
47	35.631827946236086	38.0	34.0	40.0	28.0	41.0
48	35.57844481582839	38.0	34.0	40.0	28.0	41.0
49	35.390811516663234	37.0	34.0	40.0	28.0	41.0
50	35.26224813789841	37.0	34.0	40.0	28.0	41.0
51	33.99689024706862	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	6.0
12	3.0
13	6.0
14	7.0
15	27.0
16	54.0
17	68.0
18	182.0
19	292.0
20	506.0
21	836.0
22	1194.0
23	1674.0
24	1983.0
25	2655.0
26	3319.0
27	4301.0
28	5804.0
29	7340.0
30	9664.0
31	12494.0
32	16421.0
33	22196.0
34	33611.0
35	40820.0
36	49358.0
37	70683.0
38	106392.0
39	128045.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.53148504645398	33.35801391215381	19.768374370404846	26.34212667098737
2	24.284535662154287	31.171955682655195	20.006269508074396	24.53723914711612
3	21.751731326577904	29.584962411798983	22.087515409335413	26.575790852287696
4	23.044288497375845	30.971562203712853	18.766406975693155	27.21774232321814
5	19.022956784627013	34.12766333895538	17.90925367852809	28.94012619788952
6	20.75187941005083	36.42334180165661	20.142429376683967	22.68234941160859
7	82.36364300728685	6.209120788034856	6.3133561676766465	5.113880037001636
8	84.2377643626545	4.261534644801597	4.689822819086229	6.810878173457673
9	77.38996147906542	7.788613727145624	9.015206441823388	5.806218351965567
10	36.99798260307667	31.916027055042818	13.842727659107998	17.243262682772507
11	27.962582960400173	27.63833784955873	23.873363629545153	20.525715560495943
12	28.22297909330605	22.355411873986732	29.33033576485114	20.091273267856078
13	26.587522140402363	23.66489287026157	27.005617556161138	22.741967433174928
14	21.748077318804484	25.64805751023603	29.596116751317847	23.007748419641636
15	20.284935679847376	27.46256084403733	27.67007002232791	24.582433453787377
16	22.868319175655845	26.025766524288574	27.667954544143296	23.43795975591228
17	22.144056371724133	25.318235229635157	28.102396836783168	24.435311561857546
18	23.655854008927317	25.201691613282897	26.131925065916377	25.01052931187341
19	23.65835411950913	28.76357992757372	25.297849712583442	22.280216240333708
20	25.354582991170766	27.118122532342774	25.357852366546986	22.169442109939478
21	24.525315542802854	26.62713927731419	25.404200570409845	23.44334460947311
22	23.433728799543058	25.642288024278	24.953026768491686	25.97095640768726
23	24.611665515974746	26.568482836740852	24.090103985368582	24.729747661915816
24	22.019050842633426	27.34274785230885	25.75883164063026	24.879369664427465
25	24.327806806839533	26.086153810649314	23.61412139383088	25.97191798868027
26	25.9249928362216	26.723489692813335	23.435844277727668	23.915673193237392
27	23.21121895776159	25.110726051344578	25.73806149118134	25.93999349971249
28	24.863984368539377	26.660025347274974	25.076301451794986	23.399688832390662
29	24.67551449391031	27.769497497004675	23.96048286751145	23.594505141573567
30	24.908024778019026	24.258765291541742	25.859028380101428	24.974181550337804
31	26.562521034584226	26.839648676768395	22.334064775942014	24.26376551270537
32	25.099956344222917	24.79244274265977	25.884798750713973	24.222802162403337
33	25.039761374060777	25.412277850751092	23.89240293320666	25.655557841981473
34	23.181794579375627	25.50632047186702	27.464291689824744	23.847593258932605
35	26.521942316679393	23.72470320802651	26.744452158460856	23.008902316833243
36	24.331653130811556	27.853539675793353	26.837725514782385	20.97708167861271
37	23.264682860972698	28.37106256622889	24.898408968088972	23.46584560470944
38	23.933943232104497	26.723297376614735	26.368473990195717	22.974285401085048
39	23.636045440471406	23.83778513280395	27.325631710633353	25.200537716091286
40	25.47420366670064	23.727972583402725	26.33693413362514	24.46088961627149
41	23.254297786248237	23.65989264909794	28.826082692119076	24.259726872534745
42	27.059850724166644	24.012985189729548	24.604742132825105	24.322421953278702
43	23.4243053058116	23.130638470547733	27.799883456383647	25.645172767257012
44	24.51031487931197	22.33521867313362	26.404821751731326	26.749644695823083
45	26.476555693809534	23.539310392575054	26.80272396663699	23.181409946978423
46	22.529265717522122	23.598928414141398	29.653619294699574	24.21818657363691
47	24.42454185473588	25.319581443025363	26.092115612805948	24.163761089432803
48	23.207180317590968	23.73835765812719	29.122826586560556	23.931635437721283
49	23.87797921831158	23.180448365985416	28.156822320987274	24.784750094715726
50	22.374643493846843	23.064866330626163	27.14870080792035	27.411789367606644
51	23.072366662371603	21.54133740530831	29.431301769116715	25.95499416320337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	810.0
1	707.0
2	604.0
3	418.0
4	232.0
5	224.5
6	217.0
7	232.0
8	247.0
9	276.0
10	305.0
11	319.5
12	334.0
13	326.5
14	319.0
15	405.5
16	492.0
17	490.0
18	488.0
19	575.5
20	663.0
21	716.5
22	770.0
23	1159.5
24	1549.0
25	1733.5
26	2559.5
27	3201.0
28	4272.5
29	5344.0
30	5687.0
31	6030.0
32	7165.0
33	8300.0
34	10266.0
35	12232.0
36	12937.5
37	13643.0
38	14653.0
39	15663.0
40	16801.0
41	17939.0
42	20191.0
43	22443.0
44	30184.5
45	37926.0
46	41021.5
47	44117.0
48	47449.5
49	50782.0
50	48376.0
51	45970.0
52	42991.5
53	40013.0
54	36479.5
55	32946.0
56	30534.5
57	28123.0
58	27319.5
59	26516.0
60	25413.5
61	24311.0
62	22731.5
63	21152.0
64	18473.5
65	15795.0
66	13825.0
67	11855.0
68	10529.5
69	9204.0
70	7676.0
71	6148.0
72	5228.0
73	4308.0
74	3703.5
75	2563.5
76	2028.0
77	1437.5
78	847.0
79	714.0
80	581.0
81	430.0
82	279.0
83	210.0
84	141.0
85	97.0
86	53.0
87	39.0
88	25.0
89	17.5
90	10.0
91	6.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.188205345020016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94330226189406	21.12517193392173
2	9.757121217376252	5.50071472903307
3	3.3937003978088356	2.8698697107873476
4	1.7159598465267638	1.9347931407082182
5	1.009626921017335	1.4229785485748474
6	0.7121616725668061	1.2044735679099627
7	0.5897862707281374	1.1637511556270816
8	0.4412423229358862	0.9950263364664317
9	0.37258872682151895	0.9452346786796089
>10	6.160654263550902	42.121024821492526
>50	0.8285347572090715	14.480621927753884
>100	0.06847394687678277	3.3833733891153095
>500	0.004108436812606966	0.6855920062831639
>1k	0.0027389578750713107	2.16737405364686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	3336	0.6415668385332428	TruSeq Adapter, Index 16 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	2812	0.5407931504662706	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	2665	0.51252266927191	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT	2416	0.4646359358202382	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	683	0.13135196364454582	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	616	0.11846677833827264	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	609	0.11712056494806501	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	574	0.1103894979970268	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	564	0.10846633601101588	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9231619860109197E-4	0.0	0.0	0.7311861870813516	0.0
2	1.9231619860109197E-4	0.0	0.0	0.9858128340291974	0.0
3	1.9231619860109197E-4	0.0	0.0	1.3233277625741138	0.0
4	1.9231619860109197E-4	0.0	0.0	2.9572461858889914	0.0
5	1.9231619860109197E-4	0.0	0.0	3.18071760866346	0.0
6	1.9231619860109197E-4	0.0	0.0	4.444235033472634	0.0
7	1.9231619860109197E-4	0.0	0.0	5.327927966044652	0.0
8	1.9231619860109197E-4	0.0	0.0	6.065652903878441	0.0
9	1.9231619860109197E-4	0.0	0.0	7.466484094488795	0.0
10	1.9231619860109197E-4	0.0	0.0	9.046746298393968	0.0
11	1.9231619860109197E-4	0.0	0.0	10.901251401504297	0.0
12	1.9231619860109197E-4	0.0	0.0	11.583396957942371	0.0
13	1.9231619860109197E-4	0.0	0.0	11.905718906797802	0.0
14	1.9231619860109197E-4	0.0	0.0	12.302082592114651	0.0
15	1.9231619860109197E-4	0.0	0.0	12.607480715493185	0.0
16	1.9231619860109197E-4	0.0	0.0	13.136734894043391	0.0
17	1.9231619860109197E-4	0.0	0.0	13.776955519186426	0.0
18	1.9231619860109197E-4	0.0	0.0	14.691034411137416	0.0
19	1.9231619860109197E-4	0.0	0.0	15.06355088782773	0.0
20	1.9231619860109197E-4	0.0	0.0	15.507608990397653	0.0
21	1.9231619860109197E-4	0.0	0.0	15.991476546078	0.0
22	1.9231619860109197E-4	0.0	0.0	16.490729397646433	0.0
23	1.9231619860109197E-4	0.0	0.0	16.99325162459109	0.0
24	1.9231619860109197E-4	0.0	0.0	17.357113872344353	0.0
25	1.9231619860109197E-4	0.0	0.0	17.69443648469067	0.0
26	1.9231619860109197E-4	0.0	0.0	17.996565232692983	0.0
27	1.9231619860109197E-4	0.0	0.0	18.336387955621113	0.0
28	1.9231619860109197E-4	0.0	0.0	18.65313273471711	0.0
29	1.9231619860109197E-4	0.0	0.0	18.98930144987182	0.0
30	1.9231619860109197E-4	0.0	0.0	19.470668894970355	0.0
31	1.9231619860109197E-4	0.0	0.0	19.791259998038374	0.0
32	1.9231619860109197E-4	0.0	0.0	20.1480065464434	0.0
33	1.9231619860109197E-4	0.0	0.0	20.483790629200907	0.0
34	1.9231619860109197E-4	0.0	0.0	20.79207349555846	0.0
35	1.9231619860109197E-4	0.0	0.0	21.152666367935506	0.0
36	1.9231619860109197E-4	0.0	0.0	21.48325791333078	0.0
37	1.9231619860109197E-4	0.0	0.0	21.82942707081275	0.0
38	1.9231619860109197E-4	0.0	0.0	22.15963398381082	0.0
39	1.9231619860109197E-4	0.0	0.0	22.517726745606055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATC	60	0.0	45.000004	8
TAAACCG	30	2.1631986E-6	45.000004	1
TCGATGG	30	2.1631986E-6	45.000004	1
CTAACCG	35	1.21021E-7	45.000004	1
TAACCGG	60	0.0	45.000004	2
CGTGGTC	20	7.029719E-4	45.0	37
CAGGTCG	20	7.029719E-4	45.0	27
CAGGTAA	40	6.8030204E-9	45.0	5
CGGGTAT	55	1.8189894E-12	45.0	6
CTATGCG	20	7.029719E-4	45.0	1
ACCGGCG	25	3.887849E-5	45.0	20
CTCAACG	40	6.8030204E-9	45.0	1
ACGTTTG	20	7.029719E-4	45.0	20
ACGCATG	20	7.029719E-4	45.0	1
CCGATGT	20	7.029719E-4	45.0	12
TTTCGTG	20	7.029719E-4	45.0	15
ATAGCGA	20	7.029719E-4	45.0	28
TTATCGT	20	7.029719E-4	45.0	22
AGTGATA	25	3.887849E-5	45.0	40
AATTGCG	40	6.8030204E-9	45.0	1
>>END_MODULE