Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553543_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 525100 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT | 3150 | 0.5998857360502761 | TruSeq Adapter, Index 16 (95% over 22bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC | 2827 | 0.538373643115597 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT | 2783 | 0.5299942868025138 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT | 2408 | 0.4585793182251 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT | 721 | 0.13730717958484098 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 638 | 0.12150066653970672 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 626 | 0.11921538754522949 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 561 | 0.10683679299181108 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CATATCG | 20 | 7.0297817E-4 | 45.000004 | 1 |
CTCGTAA | 20 | 7.0297817E-4 | 45.000004 | 33 |
CTCAACG | 30 | 2.1632404E-6 | 45.000004 | 1 |
CTATACG | 20 | 7.0297817E-4 | 45.000004 | 1 |
TAAACCG | 30 | 2.1632404E-6 | 45.000004 | 1 |
CTAAGCG | 20 | 7.0297817E-4 | 45.000004 | 1 |
CGACACG | 40 | 6.8030204E-9 | 45.000004 | 1 |
ATCGTAG | 30 | 2.1632404E-6 | 45.000004 | 1 |
ATTCCCG | 20 | 7.0297817E-4 | 45.000004 | 1 |
ACGATAA | 20 | 7.0297817E-4 | 45.000004 | 17 |
TCAATCG | 20 | 7.0297817E-4 | 45.000004 | 1 |
TACGACG | 20 | 7.0297817E-4 | 45.000004 | 1 |
CATACCG | 20 | 7.0297817E-4 | 45.000004 | 1 |
CATCGCG | 40 | 6.8030204E-9 | 45.000004 | 1 |
CGTAACG | 30 | 2.1632404E-6 | 45.000004 | 1 |
ACGAGTA | 20 | 7.0297817E-4 | 45.000004 | 26 |
ATACACG | 40 | 6.8030204E-9 | 45.000004 | 1 |
GTGCCGT | 20 | 7.0297817E-4 | 45.000004 | 43 |
GGTAATC | 75 | 0.0 | 45.0 | 8 |
TACGCGG | 25 | 3.8879018E-5 | 45.0 | 2 |