FastQCFastQC Report
Sat 18 Jun 2016
SRR3553543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525100
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT31500.5998857360502761TruSeq Adapter, Index 16 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC28270.538373643115597No Hit
AATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT27830.5299942868025138No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGT24080.4585793182251No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT7210.13730717958484098No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6380.12150066653970672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6260.11921538754522949No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5610.10683679299181108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG207.0297817E-445.0000041
CTCGTAA207.0297817E-445.00000433
CTCAACG302.1632404E-645.0000041
CTATACG207.0297817E-445.0000041
TAAACCG302.1632404E-645.0000041
CTAAGCG207.0297817E-445.0000041
CGACACG406.8030204E-945.0000041
ATCGTAG302.1632404E-645.0000041
ATTCCCG207.0297817E-445.0000041
ACGATAA207.0297817E-445.00000417
TCAATCG207.0297817E-445.0000041
TACGACG207.0297817E-445.0000041
CATACCG207.0297817E-445.0000041
CATCGCG406.8030204E-945.0000041
CGTAACG302.1632404E-645.0000041
ACGAGTA207.0297817E-445.00000426
ATACACG406.8030204E-945.0000041
GTGCCGT207.0297817E-445.00000443
GGTAATC750.045.08
TACGCGG253.8879018E-545.02