FastQCFastQC Report
Sat 18 Jun 2016
SRR3553541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975526
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT41530.42571904798026905TruSeq Adapter, Index 4 (96% over 27bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG12910.13233886129124184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG453.8380676E-1045.01
CGTAACG502.1827873E-1145.01
TTACGCA253.890371E-545.015
TAGTGCG253.890371E-545.01
TCGACAA406.8139343E-945.033
CGAAACG302.165163E-644.9999961
CATACCG302.165163E-644.9999961
GCTACGA1600.042.18759
TCGTTGA2150.041.86046224
ATTACGG1350.041.6666642
CGTAAGG1300.041.538462
TATACGG1300.041.538462
CGGGTAC1700.041.029416
TACGGGT1650.040.9090924
AACGGGA8700.040.862074
CGAATAT1600.040.7812513
TTAATCG2250.040.020
ACTACGG2200.039.8863642
TCTAACG1700.039.7058831
TACGAAT1650.039.54545611
Produced by FastQC (version 0.11.5)