FastQCFastQC Report
Sat 18 Jun 2016
SRR3553534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429557
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT42880.2999530623822625TruSeq Adapter, Index 19 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT23090.16151856833970243No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18600.1301102369475299No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC17840.1247939046851577No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGT16960.11863815153925307No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG15600.10912471485921864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG650.045.0000041
CGTATAC253.8912844E-545.024
GCGATAT253.8912844E-545.010
CAATGCG406.8175723E-945.01
TCGAGTG207.0338644E-445.029
CGCACGA253.8912844E-545.039
TATAGCG1100.042.9545481
TACGCGG950.042.631582
CCCGATC1550.042.0967741
TAATACG556.184564E-1140.9090921
CGATACG556.184564E-1140.9090921
CGGGTAT2650.040.754726
TTACCGG1950.040.3846172
GACCGAT2400.040.3124969
ACGTAAG950.040.2631571
TTACCCG950.040.2631571
TCACGCG1400.040.1785741
GATCGTA451.9288564E-840.09
CGTAACG451.9288564E-840.01
CAACGTA403.4590084E-739.37519