##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553534_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429557 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1023484897769 33.0 31.0 34.0 30.0 34.0 2 32.30733576905293 34.0 31.0 34.0 30.0 34.0 3 32.43264941516848 34.0 31.0 34.0 30.0 34.0 4 36.01472763940158 37.0 35.0 37.0 35.0 37.0 5 35.82066962002914 37.0 35.0 37.0 35.0 37.0 6 35.82180843436113 37.0 35.0 37.0 35.0 37.0 7 35.835526670150266 37.0 35.0 37.0 35.0 37.0 8 35.98891055061113 37.0 35.0 37.0 35.0 37.0 9 37.633072343390296 39.0 37.0 39.0 35.0 39.0 10 37.25420812181676 39.0 37.0 39.0 34.0 39.0 11 37.2242764716622 39.0 37.0 39.0 34.0 39.0 12 37.135810604264115 39.0 37.0 39.0 34.0 39.0 13 37.11372544081838 39.0 37.0 39.0 33.0 39.0 14 38.36206461162444 40.0 38.0 41.0 34.0 41.0 15 38.35588297633463 40.0 38.0 41.0 34.0 41.0 16 38.292217099423105 40.0 38.0 41.0 33.0 41.0 17 38.30062389957168 40.0 38.0 41.0 34.0 41.0 18 38.27088881380735 40.0 38.0 41.0 34.0 41.0 19 38.274151362974685 40.0 38.0 41.0 34.0 41.0 20 38.19718136457658 40.0 37.0 41.0 34.0 41.0 21 38.166010869101406 40.0 37.0 41.0 34.0 41.0 22 38.14198804244951 40.0 37.0 41.0 34.0 41.0 23 38.05620202622211 40.0 37.0 41.0 33.0 41.0 24 37.95659424562994 40.0 37.0 41.0 33.0 41.0 25 37.948337142205595 40.0 37.0 41.0 33.0 41.0 26 37.88769667806181 40.0 37.0 41.0 33.0 41.0 27 37.83556864119444 40.0 37.0 41.0 33.0 41.0 28 37.774177594877294 40.0 37.0 41.0 33.0 41.0 29 37.67459639594644 40.0 37.0 41.0 33.0 41.0 30 37.68361527382259 40.0 37.0 41.0 33.0 41.0 31 37.633787949693506 40.0 36.0 41.0 33.0 41.0 32 37.6161782985918 40.0 36.0 41.0 33.0 41.0 33 37.54234073912408 40.0 36.0 41.0 33.0 41.0 34 37.48005990667039 40.0 36.0 41.0 32.0 41.0 35 37.41671231017721 40.0 36.0 41.0 32.0 41.0 36 37.34258585002207 39.0 36.0 41.0 32.0 41.0 37 37.250272636907795 39.0 36.0 41.0 32.0 41.0 38 37.241348893398445 39.0 36.0 41.0 32.0 41.0 39 37.16222368188187 39.0 35.0 41.0 32.0 41.0 40 37.049822427507266 39.0 35.0 41.0 31.0 41.0 41 36.9993116748755 39.0 35.0 41.0 31.0 41.0 42 36.94280955568753 39.0 35.0 41.0 31.0 41.0 43 36.88345620356516 39.0 35.0 41.0 31.0 41.0 44 36.75587052492485 39.0 35.0 41.0 31.0 41.0 45 36.59583213540978 39.0 35.0 40.0 31.0 41.0 46 36.48753634867305 39.0 35.0 40.0 30.0 41.0 47 36.3913072371371 38.0 35.0 40.0 30.0 41.0 48 36.313436260324 38.0 35.0 40.0 30.0 41.0 49 36.15143852256328 38.0 35.0 40.0 30.0 41.0 50 36.03327534334063 38.0 35.0 40.0 30.0 41.0 51 34.80008632044753 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 9.0 9 9.0 10 14.0 11 13.0 12 27.0 13 28.0 14 32.0 15 54.0 16 107.0 17 179.0 18 341.0 19 602.0 20 997.0 21 1602.0 22 2256.0 23 3225.0 24 4223.0 25 5490.0 26 7200.0 27 9403.0 28 12307.0 29 16261.0 30 21414.0 31 28491.0 32 37125.0 33 51444.0 34 81432.0 35 100635.0 36 123185.0 37 184680.0 38 304283.0 39 432364.0 40 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.740830900761566 31.71961663648249 20.729708574054758 26.809843888701185 2 23.878166452964102 30.26916730147871 19.50905070591799 26.343615539639202 3 21.288762882487372 29.871771464866388 21.028332553371428 27.81113309927481 4 21.72819971501661 30.869982798867063 18.967694187779852 28.434123298336477 5 19.002320299225563 33.036668002744904 17.417423719376004 30.543587978653523 6 21.5966204915229 36.169596595308896 19.269116236708296 22.96466667645991 7 86.67314419781793 4.502653619268067 4.453127787139652 4.371074395774356 8 87.86931895685167 3.744516657957675 3.922264030045672 4.463900355144985 9 80.06340425740281 8.212544165780027 6.966214008955222 4.757837567861932 10 35.4451064210801 36.30320441927115 12.661404896761724 15.590284262887034 11 26.526119630067218 27.03347960242229 25.80897438856933 20.631426378941168 12 27.774338483879973 22.702137795135137 29.891427903889106 19.632095817095784 13 25.995115969492648 23.4916830878377 28.08786218387934 22.42533875879031 14 21.826551861870495 26.364810916948393 28.57787412464141 23.230763096539697 15 20.776576240051988 28.976808899540206 28.41362743843023 21.832987421977577 16 23.21516385845405 25.493352136361125 28.65545060462787 22.636033400556958 17 22.294668907920425 26.440848458648382 27.732297487963052 23.53218514546814 18 23.322120069364146 26.841601978794827 26.20371205905046 23.632565892790563 19 24.139296299482986 28.555839326448684 25.939364432478033 21.365499941590297 20 26.102841649545976 26.669660601151268 26.02414594171481 21.203351807587943 21 25.591704283215012 26.325358135422373 25.555469281742525 22.527468299620093 22 23.45600770028757 26.1634198566409 25.857940606775387 24.52263183629614 23 23.63228608582939 27.114483717683168 25.282237784152713 23.97099241233473 24 22.406381837170535 27.18541478234166 26.50289565228949 23.905307728198316 25 23.71350005631115 27.03173080891493 24.714859218625072 24.53990991614885 26 25.01257382531791 27.713410518083574 24.23680902545334 23.037206631145175 27 23.490983570434757 25.91774934472707 26.12795432431166 24.463312760526513 28 23.19110045979279 27.104760425782253 26.91798927919628 22.786149835228674 29 24.53816112264149 27.48557769994481 24.388464398411536 23.587796779002165 30 23.589685475990112 25.865425442986883 26.3399080974036 24.2049809836194 31 24.878406387433312 27.16617805376071 23.97462990283004 23.980785655975943 32 24.870991502962106 26.725202282945 24.977528003430432 23.426278210662463 33 23.898732264610643 26.86629494311874 25.578483404299373 23.656489387971238 34 23.1549354100606 26.24085643314677 27.41667523575485 23.187532921037775 35 24.6709994774605 24.90260968957516 26.759128876987763 23.66726195597657 36 24.18077768147755 28.168936250880517 25.081965951689927 22.568320115952005 37 23.72462238301796 29.0005225395 24.35698611527907 22.917868962202974 38 23.416484967021255 26.61523814720225 26.15243743341469 23.815839452361814 39 23.534003890715795 25.151428029802243 26.710722272704064 24.603845806777905 40 23.3425459775301 25.344424881274406 27.777206505232037 23.53582263596345 41 21.123676775392656 25.108477661261496 30.561005961986826 23.20683960135902 42 23.405572495535328 25.925513987899745 27.273833782073748 23.395079734491176 43 22.913811761265904 25.668511294058227 27.23375143488507 24.183925509790797 44 23.85018575684635 24.82706181005724 26.180558033013025 25.142194400083383 45 24.08158611374013 25.050627572038053 26.79711267196761 24.07067364225421 46 21.690425775257648 25.351420055303848 28.043792587493886 24.914361581944615 47 22.842600889646235 25.879835501487523 27.233961290105956 24.04360231876029 48 22.32691666019613 24.536971943056486 28.664334475645255 24.47177692110213 49 21.919098014279946 26.000921963937078 27.5192244870264 24.56075553475657 50 21.871111120438012 25.02691393207826 27.186534010186374 25.915440937297358 51 21.959320264949213 23.968544101424428 28.97897740348933 25.093158230137032 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 999.0 1 862.0 2 725.0 3 541.5 4 358.0 5 379.0 6 400.0 7 412.5 8 425.0 9 525.5 10 626.0 11 758.5 12 891.0 13 828.0 14 765.0 15 927.5 16 1090.0 17 1298.0 18 1506.0 19 1594.5 20 1683.0 21 2042.0 22 2401.0 23 3889.5 24 5378.0 25 6440.0 26 9424.0 27 11346.0 28 15019.5 29 18693.0 30 21624.5 31 24556.0 32 28155.0 33 31754.0 34 36622.0 35 41490.0 36 44260.5 37 47031.0 38 52633.5 39 58236.0 40 60687.5 41 63139.0 42 68639.5 43 74140.0 44 80527.5 45 86915.0 46 96289.5 47 105664.0 48 114403.5 49 123143.0 50 121909.0 51 120675.0 52 110996.0 53 101317.0 54 94735.5 55 88154.0 56 82685.5 57 77217.0 58 75884.5 59 74552.0 60 73043.5 61 71535.0 62 64243.0 63 56951.0 64 49450.0 65 41949.0 66 35446.5 67 28944.0 68 24640.5 69 20337.0 70 16952.0 71 13567.0 72 11624.5 73 9682.0 74 7972.5 75 4673.0 76 3083.0 77 2356.0 78 1629.0 79 1549.0 80 1469.0 81 1149.0 82 829.0 83 545.0 84 261.0 85 215.5 86 170.0 87 109.5 88 49.0 89 39.5 90 30.0 91 19.0 92 8.0 93 11.5 94 15.0 95 12.5 96 10.0 97 7.0 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1429557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.452308127988005 #Duplication Level Percentage of deduplicated Percentage of total 1 74.00911672189022 16.61675492920109 2 10.073345028186006 4.523396929047364 3 3.70212864750235 2.4936399936952274 4 1.7653626339356343 1.5854586325903741 5 1.069583186677203 1.2007305637895938 6 0.7341480869566257 0.9889991435953855 7 0.5189691634103684 0.8156438896069625 8 0.4349100540111352 0.7811787632494331 9 0.3435253539343734 0.694164338667962 >10 5.184094549139369 29.405588801846655 >50 1.7887763928231197 28.23849521790509 >100 0.37040427539916804 11.084192562000709 >500 0.003131058963589787 0.46425790207741974 >1k 0.0025048471708718294 1.1074983327267822 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT 4288 0.2999530623822625 TruSeq Adapter, Index 19 (95% over 22bp) AATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT 2309 0.16151856833970243 No Hit ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA 1860 0.1301102369475299 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC 1784 0.1247939046851577 No Hit AATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGT 1696 0.11863815153925307 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 1560 0.10912471485921864 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.33828661606357774 0.0 2 0.0 0.0 0.0 0.4998051844032802 0.0 3 0.0 0.0 0.0 0.6923123736933889 0.0 4 0.0 0.0 0.0 1.507599906824282 0.0 5 0.0 0.0 0.0 1.6491822291800886 0.0 6 0.0 0.0 0.0 2.4974170319896305 0.0 7 0.0 0.0 0.0 3.114181526165099 0.0 8 0.0 0.0 0.0 3.702615565521347 0.0 9 0.0 0.0 0.0 4.5096487932975045 0.0 10 0.0 0.0 0.0 5.620062718730348 0.0 11 0.0 0.0 0.0 6.700117588875435 0.0 12 0.0 0.0 0.0 7.177538216384517 0.0 13 0.0 0.0 0.0 7.399704943559438 0.0 14 0.0 0.0 0.0 7.667060494964524 0.0 15 0.0 0.0 0.0 7.877265474549109 0.0 16 0.0 0.0 0.0 8.258292603932547 0.0 17 0.0 0.0 0.0 8.740190142820468 0.0 18 0.0 0.0 0.0 9.287492558883626 0.0 19 0.0 0.0 0.0 9.569677879231119 0.0 20 0.0 0.0 0.0 9.878025150448705 0.0 21 0.0 0.0 0.0 10.249049180970049 0.0 22 0.0 0.0 0.0 10.670648319724222 0.0 23 0.0 0.0 0.0 11.082943877019245 0.0 24 0.0 0.0 0.0 11.399545453591568 0.0 25 0.0 0.0 0.0 11.679142559548168 0.0 26 0.0 0.0 0.0 11.933557038998794 0.0 27 0.0 0.0 0.0 12.22714449301427 0.0 28 0.0 0.0 0.0 12.509819475543821 0.0 29 0.0 0.0 0.0 12.81033215184844 0.0 30 0.0 0.0 0.0 13.18884101858128 0.0 31 0.0 0.0 0.0 13.481519099972928 0.0 32 6.995174029437091E-5 0.0 0.0 13.794133427348472 0.0 33 6.995174029437091E-5 0.0 0.0 14.08744107440277 0.0 34 6.995174029437091E-5 0.0 0.0 14.39054196509828 0.0 35 6.995174029437091E-5 0.0 0.0 14.705814458605008 0.0 36 6.995174029437091E-5 0.0 0.0 15.020527338189384 0.0 37 6.995174029437091E-5 0.0 0.0 15.325237118911662 0.0 38 6.995174029437091E-5 0.0 0.0 15.643447585510756 0.0 39 6.995174029437091E-5 0.0 0.0 15.965085687384274 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGCG 65 0.0 45.000004 1 CGTATAC 25 3.8912844E-5 45.0 24 GCGATAT 25 3.8912844E-5 45.0 10 CAATGCG 40 6.8175723E-9 45.0 1 TCGAGTG 20 7.0338644E-4 45.0 29 CGCACGA 25 3.8912844E-5 45.0 39 TATAGCG 110 0.0 42.954548 1 TACGCGG 95 0.0 42.63158 2 CCCGATC 155 0.0 42.09677 41 TAATACG 55 6.184564E-11 40.909092 1 CGATACG 55 6.184564E-11 40.909092 1 CGGGTAT 265 0.0 40.75472 6 TTACCGG 195 0.0 40.384617 2 GACCGAT 240 0.0 40.312496 9 ACGTAAG 95 0.0 40.263157 1 TTACCCG 95 0.0 40.263157 1 TCACGCG 140 0.0 40.178574 1 GATCGTA 45 1.9288564E-8 40.0 9 CGTAACG 45 1.9288564E-8 40.0 1 CAACGTA 40 3.4590084E-7 39.375 19 >>END_MODULE