FastQCFastQC Report
Sat 18 Jun 2016
SRR3553533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440455
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT43200.29990523827540605TruSeq Adapter, Index 19 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT23540.16342058585655225No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18910.1312779642543502No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC17550.12183650304938369No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGT16810.11669923739374018No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG15280.10607759353815287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG207.0338824E-445.01
TACGTCG551.8189894E-1245.01
TACCGCG351.2122109E-745.01
TGCGACG302.165887E-644.9999961
CGCAATT253.8913007E-544.99999624
TATCGCA253.8913007E-544.99999633
CAATACG302.165887E-644.9999961
CCGCAAT253.8913007E-544.99999639
CGAATAG650.041.5384641
TATAGCG1300.041.5384641
GACCGAT1800.041.259
ATATACG603.6379788E-1241.2499961
CATACCG501.0822987E-940.4999961
AACGTAG950.040.2631571
AATTGCG451.9288564E-840.01
TACGCGG2150.039.7674452
CGATTGT403.4590266E-739.37541
AATACCG1200.039.3749961
CTAGCGG2750.039.2727282
AGATACG1150.039.1304321
Produced by FastQC (version 0.11.5)