##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1440455
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071886313699494	33.0	31.0	34.0	30.0	34.0
2	32.26534185378925	34.0	31.0	34.0	30.0	34.0
3	32.39918636819616	34.0	31.0	34.0	30.0	34.0
4	35.95970717585763	37.0	35.0	37.0	35.0	37.0
5	35.795335501629694	37.0	35.0	37.0	35.0	37.0
6	35.80511921580334	37.0	35.0	37.0	35.0	37.0
7	35.844086070026485	37.0	35.0	37.0	35.0	37.0
8	35.99166166246082	37.0	35.0	37.0	35.0	37.0
9	37.62887420988507	39.0	37.0	39.0	35.0	39.0
10	37.23929452846497	39.0	37.0	39.0	34.0	39.0
11	37.209128365690006	39.0	37.0	39.0	34.0	39.0
12	37.12896272358387	39.0	37.0	39.0	34.0	39.0
13	37.09997049543374	39.0	37.0	39.0	33.0	39.0
14	38.34402254843088	40.0	38.0	41.0	34.0	41.0
15	38.33287954153375	40.0	38.0	41.0	34.0	41.0
16	38.293277471354536	40.0	38.0	41.0	33.0	41.0
17	38.279461697866296	40.0	38.0	41.0	33.0	41.0
18	38.23053757319736	40.0	37.0	41.0	33.0	41.0
19	38.24255808060647	40.0	37.0	41.0	34.0	41.0
20	38.15895602431176	40.0	37.0	41.0	33.0	41.0
21	38.14893419093273	40.0	37.0	41.0	33.0	41.0
22	38.10793950522578	40.0	37.0	41.0	33.0	41.0
23	38.03779569649868	40.0	37.0	41.0	33.0	41.0
24	37.953409860078935	40.0	37.0	41.0	33.0	41.0
25	37.9520352944035	40.0	37.0	41.0	33.0	41.0
26	37.876070408308486	40.0	37.0	41.0	33.0	41.0
27	37.80270956052081	40.0	37.0	41.0	33.0	41.0
28	37.75233936499231	40.0	37.0	41.0	33.0	41.0
29	37.647285059234754	40.0	37.0	41.0	33.0	41.0
30	37.66605829408069	40.0	37.0	41.0	33.0	41.0
31	37.61215310440104	40.0	36.0	41.0	33.0	41.0
32	37.59833385978736	40.0	36.0	41.0	33.0	41.0
33	37.5314272226484	40.0	36.0	41.0	33.0	41.0
34	37.448641575057884	40.0	36.0	41.0	32.0	41.0
35	37.38921035367297	40.0	36.0	41.0	32.0	41.0
36	37.34072428503494	39.0	36.0	41.0	32.0	41.0
37	37.22044909420982	39.0	36.0	41.0	32.0	41.0
38	37.18441672943619	39.0	35.0	41.0	32.0	41.0
39	37.121438017848526	39.0	35.0	41.0	31.0	41.0
40	37.02726916148023	39.0	35.0	41.0	31.0	41.0
41	36.99305497221364	39.0	35.0	41.0	31.0	41.0
42	36.917360833903174	39.0	35.0	41.0	31.0	41.0
43	36.85989774064445	39.0	35.0	41.0	31.0	41.0
44	36.7734597748628	39.0	35.0	40.0	31.0	41.0
45	36.61718831896866	39.0	35.0	40.0	31.0	41.0
46	36.49148359372559	39.0	35.0	40.0	31.0	41.0
47	36.38523313814038	38.0	35.0	40.0	30.0	41.0
48	36.326410752158175	38.0	35.0	40.0	30.0	41.0
49	36.18075052674329	38.0	35.0	40.0	30.0	41.0
50	36.03042927408354	38.0	35.0	40.0	30.0	41.0
51	34.83191005619752	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	5.0
10	10.0
11	20.0
12	22.0
13	31.0
14	38.0
15	55.0
16	102.0
17	188.0
18	325.0
19	626.0
20	1028.0
21	1539.0
22	2229.0
23	3168.0
24	4245.0
25	5653.0
26	7332.0
27	9581.0
28	12421.0
29	16388.0
30	21707.0
31	28844.0
32	38322.0
33	52349.0
34	82513.0
35	101984.0
36	125214.0
37	187531.0
38	305566.0
39	431319.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.861186222408893	31.62965868423519	20.68360344474489	26.82555164861103
2	23.955972244880957	30.181713416941175	19.50619769447848	26.356116643699384
3	21.32451204654085	29.873477477602563	20.9897567088177	27.812253767038886
4	21.772495496214738	30.871078929921445	19.005383715562097	28.351041858301716
5	18.998719154711534	33.02657840751707	17.444557448861644	30.53014498890975
6	21.60303515208736	36.17947106990499	19.263427180994892	22.954066597012748
7	86.76827807880149	4.528707942976351	4.410411987878829	4.292601990343329
8	87.96512213154871	3.727780458257981	3.8995317451777387	4.407565665015568
9	80.08247394052573	8.190953552870447	6.967937214282988	4.75863529232083
10	35.44053788559865	36.31061018914162	12.670163247029583	15.578688678230144
11	26.571742956218696	26.94037647826555	25.816981439892256	20.670899125623503
12	27.77171102186462	22.698800031934354	29.86278641123812	19.66670253496291
13	25.960616610723697	23.588796595520165	28.073907202932407	22.376679590823734
14	21.839418794755826	26.426788757718917	28.46815763074862	23.265634816776643
15	20.760315317035243	28.99181161507996	28.35291626604094	21.89495680184386
16	23.235158335387084	25.543526177492527	28.591035471430903	22.630280015689486
17	22.319267175996472	26.479480441943693	27.7156176347057	23.485634747354133
18	23.305691604388894	26.76182178547751	26.18853070731123	23.74395590282237
19	24.122030886074192	28.539732237383326	25.91007702427358	21.4281598522689
20	26.081550621157902	26.752241479254817	25.9965774703132	21.169630429274083
21	25.617044614375317	26.34632806995012	25.629262975934687	22.407364339739875
22	23.46487741720498	26.18679514458973	25.86432759093481	24.48399984727048
23	23.614899458851543	27.252014120538306	25.155176662929424	23.97790975768073
24	22.441520214099018	27.220912836569	26.453585846138893	23.88398110319309
25	23.705148720369607	27.079568608529943	24.70337497526823	24.511907695832218
26	25.034381497512936	27.679517930098473	24.20575443175941	23.080346140629178
27	23.484871099756674	25.988316191758855	26.11084691989684	24.415965788587634
28	23.18739564929137	27.15482260813424	26.941278970880727	22.716502771693666
29	24.507117542720877	27.55469625916811	24.369175017615962	23.569011180495053
30	23.587269300325246	25.98408141871839	26.30161997424425	24.127029306712117
31	24.90456140594465	27.194115748149024	23.967218691316287	23.934104154590045
32	24.8330562218188	26.781815468029198	25.03750551041164	23.347622799740357
33	23.970898084285867	26.89483531245336	25.60350722514761	23.530759378113167
34	23.182119538618007	26.27336501313821	27.40738169536709	23.137133752876696
35	24.66810834076733	25.02910538683957	26.729401473839864	23.573384798553235
36	24.123766448795696	28.2141406708297	25.12851841952716	22.53357446084744
37	23.692374978739355	29.12406149445835	24.259348608599364	22.924214918202928
38	23.407603847395443	26.673446931698663	26.142434161428156	23.776515059477735
39	23.528399012811924	25.221058623837607	26.715516972067853	24.535025391282616
40	23.33283580535317	25.346157984803412	27.824402706089398	23.49660350375402
41	21.070564509130797	25.14649884932191	30.507374406003656	23.27556223554363
42	23.39434414820317	25.95520165503261	27.22028803398926	23.430166162774956
43	22.92706124106619	25.68861922101003	27.20800024992103	24.176319288002748
44	23.804353485530612	24.90414487089149	26.195681225723817	25.095820417854082
45	24.1635455463725	25.035492257654703	26.7282907136981	24.0726714822747
46	21.768330145683134	25.44411314480494	28.0064979468293	24.781058762682626
47	22.810709116216753	25.927502073997456	27.20633410970839	24.055454700077405
48	22.285388991672768	24.63964511213471	28.562433397780563	24.51253249841196
49	22.001728620470615	25.9091745316584	27.545393642980866	24.54370320489012
50	21.880933455054134	25.032021132211696	27.194393438184463	25.892651974549707
51	21.94084508020035	24.034350257383952	28.979176718467432	25.04562794394827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1109.0
1	905.0
2	701.0
3	554.0
4	407.0
5	392.0
6	377.0
7	418.0
8	459.0
9	549.5
10	640.0
11	763.0
12	886.0
13	877.5
14	869.0
15	946.5
16	1024.0
17	1316.5
18	1609.0
19	1639.5
20	1670.0
21	2018.5
22	2367.0
23	3921.5
24	5476.0
25	6506.5
26	9450.5
27	11364.0
28	15056.5
29	18749.0
30	21751.5
31	24754.0
32	28328.5
33	31903.0
34	36950.0
35	41997.0
36	44976.0
37	47955.0
38	53518.5
39	59082.0
40	61336.5
41	63591.0
42	69123.5
43	74656.0
44	81239.5
45	87823.0
46	97073.0
47	106323.0
48	114905.0
49	123487.0
50	122057.5
51	120628.0
52	111326.5
53	102025.0
54	95708.5
55	89392.0
56	83481.0
57	77570.0
58	76177.5
59	74785.0
60	73533.5
61	72282.0
62	64857.5
63	57433.0
64	49931.5
65	42430.0
66	35882.5
67	29335.0
68	24920.0
69	20505.0
70	17067.5
71	13630.0
72	11743.0
73	9856.0
74	8010.5
75	4594.0
76	3023.0
77	2395.5
78	1768.0
79	1613.5
80	1459.0
81	1133.5
82	808.0
83	543.0
84	278.0
85	217.0
86	156.0
87	96.5
88	37.0
89	36.0
90	35.0
91	24.5
92	14.0
93	14.0
94	14.0
95	11.5
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1440455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.04676285505868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.97521567570926	16.30914037154182
2	10.028810888636333	4.42205630759991
3	3.620393074997809	2.3945384269972014
4	1.7697111245649217	1.560656059409682
5	1.0942431164613056	1.2062259247201383
6	0.7206751750493121	0.9533132807904054
7	0.5198080384374251	0.8022059187508198
8	0.4263468096687366	0.7519653605421981
9	0.3675818325179379	0.7293590542215788
>10	5.227026941578308	28.995404178929235
>50	1.8524978778217331	28.693164784688722
>100	0.39101893470421445	11.492845022213793
>500	0.004129363242161143	0.5841908512701987
>1k	0.0025411466105607033	1.1049344583242862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	4320	0.29990523827540605	TruSeq Adapter, Index 19 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT	2354	0.16342058585655225	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1891	0.1312779642543502	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC	1755	0.12183650304938369	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGT	1681	0.11669923739374018	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1528	0.10607759353815287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.94225088600477E-5	0.0	0.0	0.33413053514340957	0.0
2	6.94225088600477E-5	0.0	0.0	0.49817592357970225	0.0
3	6.94225088600477E-5	0.0	0.0	0.6908233856663346	0.0
4	6.94225088600477E-5	0.0	0.0	1.502233669222572	0.0
5	6.94225088600477E-5	0.0	0.0	1.6470490227046315	0.0
6	6.94225088600477E-5	0.0	0.0	2.4745653283164	0.0
7	6.94225088600477E-5	0.0	0.0	3.0690302716849884	0.0
8	6.94225088600477E-5	0.0	0.0	3.6544008663929106	0.0
9	6.94225088600477E-5	0.0	0.0	4.462548292032726	0.0
10	6.94225088600477E-5	0.0	0.0	5.558521439406299	0.0
11	6.94225088600477E-5	0.0	0.0	6.641859690167343	0.0
12	6.94225088600477E-5	0.0	0.0	7.126289956992756	0.0
13	6.94225088600477E-5	0.0	0.0	7.343512987215845	0.0
14	6.94225088600477E-5	0.0	0.0	7.606277183251126	0.0
15	6.94225088600477E-5	0.0	0.0	7.822042340788154	0.0
16	6.94225088600477E-5	6.94225088600477E-5	0.0	8.19685446612355	0.0
17	6.94225088600477E-5	6.94225088600477E-5	0.0	8.657681079936548	0.0
18	6.94225088600477E-5	6.94225088600477E-5	0.0	9.193414580809536	0.0
19	6.94225088600477E-5	6.94225088600477E-5	0.0	9.47818571215345	0.0
20	6.94225088600477E-5	6.94225088600477E-5	0.0	9.789545664390765	0.0
21	6.94225088600477E-5	6.94225088600477E-5	0.0	10.148390612688353	0.0
22	6.94225088600477E-5	6.94225088600477E-5	0.0	10.560066090228435	0.0
23	6.94225088600477E-5	6.94225088600477E-5	0.0	10.955010743133245	0.0
24	6.94225088600477E-5	6.94225088600477E-5	0.0	11.278033676859048	0.0
25	6.94225088600477E-5	6.94225088600477E-5	0.0	11.564332103397884	0.0
26	6.94225088600477E-5	6.94225088600477E-5	0.0	11.827096299433165	0.0
27	6.94225088600477E-5	6.94225088600477E-5	0.0	12.1127005008834	0.0
28	6.94225088600477E-5	6.94225088600477E-5	0.0	12.40205351781208	0.0
29	6.94225088600477E-5	6.94225088600477E-5	0.0	12.702652981176087	0.0
30	6.94225088600477E-5	6.94225088600477E-5	0.0	13.078992401706405	0.0
31	6.94225088600477E-5	6.94225088600477E-5	0.0	13.372094234113527	0.0
32	6.94225088600477E-5	6.94225088600477E-5	0.0	13.676859048009137	0.0
33	6.94225088600477E-5	6.94225088600477E-5	0.0	13.960866531755592	0.0
34	6.94225088600477E-5	6.94225088600477E-5	0.0	14.257300644587994	0.0
35	6.94225088600477E-5	6.94225088600477E-5	0.0	14.567341569156968	0.0
36	6.94225088600477E-5	6.94225088600477E-5	0.0	14.871203890437396	0.0
37	6.94225088600477E-5	6.94225088600477E-5	0.0	15.172497578890004	0.0
38	1.388450177200954E-4	6.94225088600477E-5	0.0	15.481774855861516	0.0
39	1.388450177200954E-4	6.94225088600477E-5	0.0	15.79285711806339	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATG	20	7.0338824E-4	45.0	1
TACGTCG	55	1.8189894E-12	45.0	1
TACCGCG	35	1.2122109E-7	45.0	1
TGCGACG	30	2.165887E-6	44.999996	1
CGCAATT	25	3.8913007E-5	44.999996	24
TATCGCA	25	3.8913007E-5	44.999996	33
CAATACG	30	2.165887E-6	44.999996	1
CCGCAAT	25	3.8913007E-5	44.999996	39
CGAATAG	65	0.0	41.538464	1
TATAGCG	130	0.0	41.538464	1
GACCGAT	180	0.0	41.25	9
ATATACG	60	3.6379788E-12	41.249996	1
CATACCG	50	1.0822987E-9	40.499996	1
AACGTAG	95	0.0	40.263157	1
AATTGCG	45	1.9288564E-8	40.0	1
TACGCGG	215	0.0	39.767445	2
CGATTGT	40	3.4590266E-7	39.375	41
AATACCG	120	0.0	39.374996	1
CTAGCGG	275	0.0	39.272728	2
AGATACG	115	0.0	39.130432	1
>>END_MODULE