FastQCFastQC Report
Sat 18 Jun 2016
SRR3553532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485125
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT56881.1724813192476167Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10860.22385982994073692No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC10690.22035557845916004No Hit
AATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT10690.22035557845916004No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC9330.1923215666065447Illumina Single End Adapter 1 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT8840.1822210770419995No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT8830.18201494460190673No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT7010.14449884050502448No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG6010.12388559649574851No Hit
AACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT5550.11440350425148157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG302.1628985E-645.0000041
CGGGTCA302.1628985E-645.0000046
AGATCGA302.1628985E-645.00000415
CGAGTAG302.1628985E-645.0000041
ACGTTAG302.1628985E-645.0000041
CGGCAGA302.1628985E-645.00000436
TAAACCG302.1628985E-645.0000041
ATTCGCG302.1628985E-645.0000041
TAACGGC302.1628985E-645.0000043
CACTGCG302.1628985E-645.0000041
CCTCGTC302.1628985E-645.00000436
CCCAACG302.1628985E-645.0000041
TCGCTAG302.1628985E-645.0000041
TCGTCCC302.1628985E-645.00000438
GCGATGT302.1628985E-645.0000049
AGTTCGT302.1628985E-645.00000430
CCGACCG551.8189894E-1245.0000041
TATAGCG302.1628985E-645.0000041
TGCAACG302.1628985E-645.0000041
CGGAACG453.8380676E-1045.01
Produced by FastQC (version 0.11.5)