##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553532_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485125
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.765392424632825	31.0	31.0	34.0	30.0	34.0
2	31.987566091213605	33.0	31.0	34.0	30.0	34.0
3	32.146100489564546	34.0	31.0	34.0	30.0	34.0
4	35.777504766812676	37.0	35.0	37.0	35.0	37.0
5	35.57230610667354	37.0	35.0	37.0	33.0	37.0
6	35.57785106931203	37.0	35.0	37.0	33.0	37.0
7	35.73694614790003	37.0	35.0	37.0	35.0	37.0
8	35.92788456583355	37.0	35.0	37.0	35.0	37.0
9	37.51640092759598	39.0	37.0	39.0	35.0	39.0
10	37.00792373099716	39.0	37.0	39.0	33.0	39.0
11	37.03354805462509	39.0	37.0	39.0	33.0	39.0
12	37.03958361247101	39.0	37.0	39.0	33.0	39.0
13	37.008131924761656	39.0	37.0	39.0	33.0	39.0
14	38.159344498840504	40.0	37.0	41.0	33.0	41.0
15	38.157143004380316	40.0	37.0	41.0	33.0	41.0
16	38.07188662715795	40.0	37.0	41.0	33.0	41.0
17	38.10685081164648	40.0	37.0	41.0	33.0	41.0
18	38.08427106415872	40.0	37.0	41.0	33.0	41.0
19	38.07316464828652	40.0	37.0	41.0	33.0	41.0
20	38.000566864210256	40.0	37.0	41.0	33.0	41.0
21	37.94039062097398	40.0	37.0	41.0	33.0	41.0
22	37.96179953620201	40.0	37.0	41.0	33.0	41.0
23	37.84252924503994	40.0	37.0	41.0	33.0	41.0
24	37.771963926822984	40.0	37.0	41.0	33.0	41.0
25	37.79434372584385	40.0	37.0	41.0	33.0	41.0
26	37.73231641329554	40.0	37.0	41.0	33.0	41.0
27	37.724835867044575	40.0	37.0	41.0	33.0	41.0
28	37.6791033238856	40.0	37.0	41.0	33.0	41.0
29	37.53224632826591	40.0	37.0	41.0	32.0	41.0
30	37.504028858541616	39.0	37.0	41.0	32.0	41.0
31	37.438194279824785	39.0	36.0	41.0	32.0	41.0
32	37.35094666323113	39.0	36.0	41.0	32.0	41.0
33	37.21797062612729	39.0	36.0	41.0	31.0	41.0
34	37.22218191187838	39.0	36.0	41.0	31.0	41.0
35	37.12485235763978	39.0	36.0	41.0	31.0	41.0
36	36.956388559649575	39.0	35.0	41.0	31.0	41.0
37	36.929566606544704	39.0	35.0	41.0	31.0	41.0
38	36.85286678691059	39.0	35.0	40.0	31.0	41.0
39	36.743734089152284	39.0	35.0	40.0	30.0	41.0
40	36.71774078845658	39.0	35.0	40.0	30.0	41.0
41	36.673671734089154	39.0	35.0	40.0	30.0	41.0
42	36.57030249935583	39.0	35.0	40.0	30.0	41.0
43	36.46412986343726	39.0	35.0	40.0	30.0	41.0
44	36.429188353517134	39.0	35.0	40.0	30.0	41.0
45	36.31737593403762	39.0	35.0	40.0	30.0	41.0
46	36.156291677402734	39.0	35.0	40.0	29.0	41.0
47	36.14123576397836	38.0	35.0	40.0	29.0	41.0
48	36.10080906982736	38.0	35.0	40.0	29.0	41.0
49	35.92878742592116	38.0	35.0	40.0	28.0	41.0
50	35.745768616335994	38.0	34.0	40.0	28.0	41.0
51	34.49135377480031	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	3.0
14	7.0
15	8.0
16	19.0
17	46.0
18	107.0
19	261.0
20	418.0
21	658.0
22	1035.0
23	1326.0
24	1712.0
25	2065.0
26	2831.0
27	3656.0
28	4811.0
29	6361.0
30	8411.0
31	11005.0
32	14535.0
33	19285.0
34	27626.0
35	35945.0
36	45315.0
37	66280.0
38	103590.0
39	127766.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.068435970110794	32.11522803401185	22.28786395258954	25.528472043287813
2	21.428497809842824	30.58077814996135	21.87930945632569	26.111414583870136
3	20.125946920896677	31.890543674310745	21.52373099716568	26.4597784076269
4	19.65431589796444	32.33496521515073	20.30239628961608	27.708322597268747
5	17.65297603710384	34.77268745168771	19.136305076011336	28.438031435197114
6	20.626230353001805	36.50131409430559	20.47060036073177	22.401855191960834
7	84.15604225715022	4.174181911878382	6.375057974748777	5.294717856222623
8	86.09306879670187	3.631435197114146	4.693841793352229	5.581654212831745
9	77.31842308683329	8.780829683071373	9.366451945374903	4.534295284720432
10	27.707910332388558	44.552847204328785	12.9224426694151	14.81679979386756
11	19.594949755217726	26.05328523576398	36.37474877608864	17.977016232929657
12	23.422210770419998	22.98129348106158	35.491677402731256	18.10481834578717
13	24.684771965988148	25.346663231125998	30.548003091986605	19.420561710899253
14	18.772893584127804	28.880185519196083	31.815099201236798	20.53182169543932
15	18.096985313063644	30.456892553465604	30.84236021643906	20.603761917031694
16	19.30553980932749	29.648853388301983	28.639216696727647	22.406390105642878
17	18.45524349394486	28.770523061066733	31.03550631280598	21.73872713218243
18	19.113218242720947	26.966658077814998	29.918474619943314	24.00164905952074
19	19.365112084514298	32.29435712445246	29.984230868332908	18.356299922700334
20	22.28209224426694	29.336150476681265	29.41695439319763	18.964802885854162
21	20.81278021128575	29.812728678175727	28.431641329554235	20.942849780984282
22	19.240608090698274	26.97593403761917	30.004431847461994	23.779026024220563
23	20.66869363566091	28.411234217985054	26.301674826075754	24.61839732027828
24	17.09580005153311	30.003195052821436	29.46024220561711	23.440762690028343
25	19.653491368204072	28.65096624581294	28.38855964957485	23.30698273640814
26	20.88884308167998	30.920896676114403	26.65890234475651	21.531357897449112
27	17.742643648544192	28.44833805720175	28.31393970626127	25.495078587992786
28	19.892192733831486	27.380365885081165	30.45751095078588	22.269930430301468
29	19.730172635918578	31.358928111311517	25.69461479000258	23.216284462767327
30	20.0	28.635300180365885	26.69270806493172	24.671991754702397
31	24.033187322854936	29.59319762947694	23.783560937902603	22.590054109765525
32	21.782633341922185	30.489049214120072	24.45575882504509	23.27255861891265
33	22.233032723524865	28.822262303530017	26.669208966761143	22.275496006183975
34	21.150219015717596	28.007214635403248	26.90296315382633	23.939603195052822
35	21.38788971914455	29.106106673537745	27.039628961607832	22.46637464570987
36	21.296985313063644	29.275959804174185	28.329605771708323	21.09744911105385
37	22.85122391136305	31.113012110280856	23.635763978356096	22.400000000000002
38	23.548363823756763	29.096212316413293	27.650399381602682	19.705024478227262
39	23.166606544704972	28.280340118526155	27.127441381087348	21.425611955681525
40	25.359031177531566	28.341973718113888	25.241123421798505	21.057871682556044
41	23.581345014171607	25.200309198660136	29.746766297346046	21.471579489822208
42	25.376758567379543	26.254264364854418	26.618500386498322	21.750476681267713
43	20.91522803401185	26.52677145065705	28.559855707291938	23.998144808039164
44	22.571914455037362	25.318835351713474	26.38742592115434	25.721824272094825
45	23.896315382633343	26.165627415614534	28.251069312032982	21.686987889719145
46	19.387580520484413	28.021231641329553	31.295027054882762	21.29616078330327
47	22.05658335480546	27.51971141458387	27.007678433393455	23.416026797217214
48	23.141252254573562	23.907446534398353	31.174439577428497	21.776861633599587
49	23.451893841793353	23.533728420510176	28.64313321308941	24.37124452460706
50	21.666786910590055	23.965163617624324	26.544704972945116	27.82334449884051
51	21.26936356609121	22.97943828910075	31.9544447307395	23.79675341406854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1434.0
1	1178.5
2	923.0
3	642.5
4	362.0
5	341.5
6	321.0
7	343.5
8	366.0
9	435.5
10	505.0
11	561.0
12	617.0
13	754.0
14	891.0
15	900.5
16	910.0
17	1309.0
18	1708.0
19	1710.0
20	1712.0
21	1749.0
22	1786.0
23	2124.5
24	2463.0
25	2859.5
26	4213.0
27	5170.0
28	5842.0
29	6514.0
30	8056.0
31	9598.0
32	11494.5
33	13391.0
34	14683.5
35	15976.0
36	18001.0
37	20026.0
38	21011.0
39	21996.0
40	22703.0
41	23410.0
42	25641.0
43	27872.0
44	33846.0
45	39820.0
46	44975.0
47	50130.0
48	54433.5
49	58737.0
50	54287.0
51	49837.0
52	42515.0
53	35193.0
54	29700.5
55	24208.0
56	20854.5
57	17501.0
58	15350.0
59	13199.0
60	12347.0
61	11495.0
62	9980.5
63	8466.0
64	7111.5
65	5757.0
66	4656.0
67	3555.0
68	2955.5
69	2356.0
70	1882.0
71	1408.0
72	1116.0
73	824.0
74	749.5
75	497.0
76	319.0
77	230.0
78	141.0
79	120.5
80	100.0
81	90.5
82	81.0
83	61.5
84	42.0
85	48.0
86	54.0
87	33.5
88	13.0
89	9.0
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.023316168241653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0079152054856	21.919397209984716
2	11.781275432210828	7.074259143328071
3	4.3998772052609985	3.962967133049713
4	2.2493912417507897	2.7013673774863065
5	1.3133818722470623	1.9716039600055357
6	0.8370043970235245	1.5077788587627443
7	0.5988497147923963	1.258561802713142
8	0.47615929643747096	1.1436704882711752
9	0.3504375858060606	0.9469168592301606
>10	3.8236044302743766	26.82024343897161
>50	0.9855466297554643	20.431786501447675
>100	0.16966023157441865	7.472508576365038
>500	0.0041380544286443565	0.943588494305461
>1k	0.0020690272143221783	0.6675728122977411
>5k	6.896757381073929E-4	1.1777773437808785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	5688	1.1724813192476167	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1086	0.22385982994073692	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	1069	0.22035557845916004	No Hit
AATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	1069	0.22035557845916004	No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	933	0.1923215666065447	Illumina Single End Adapter 1 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	884	0.1822210770419995	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT	883	0.18201494460190673	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT	701	0.14449884050502448	No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	601	0.12388559649574851	No Hit
AACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	555	0.11440350425148157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3229580005153312	0.0
2	0.0	0.0	0.0	1.8073692347333161	0.0
3	0.0	0.0	0.0	2.331151765009018	0.0
4	0.0	0.0	0.0	4.600876062870395	0.0
5	0.0	0.0	0.0	4.8855449626384955	0.0
6	0.0	0.0	0.0	6.6069569698531305	0.0
7	0.0	0.0	0.0	7.882092244266942	0.0
8	0.0	0.0	0.0	9.046122133470755	0.0
9	0.0	0.0	0.0	10.882349909817057	0.0
10	0.0	0.0	0.0	13.473846946663231	0.0
11	0.0	0.0	0.0	15.801906725070857	0.0
12	0.0	0.0	0.0	16.932543158979644	0.0
13	0.0	0.0	0.0	17.359237309971657	0.0
14	0.0	0.0	0.0	17.820767843339347	0.0
15	0.0	0.0	0.0	18.257562483895903	0.0
16	0.0	0.0	0.0	19.056119556815254	0.0
17	0.0	0.0	0.0	20.274362277763462	0.0
18	0.0	0.0	0.0	21.78593146096367	0.0
19	0.0	0.0	0.0	22.464519453749034	0.0
20	0.0	0.0	0.0	23.183303272352486	0.0
21	0.0	0.0	0.0	23.88435970110796	0.0
22	0.0	0.0	0.0	24.681679979386757	0.0
23	0.0	0.0	0.0	25.451790775573304	0.0
24	0.0	0.0	0.0	26.0071115691832	0.0
25	0.0	0.0	0.0	26.480185519196084	0.0
26	0.0	0.0	0.0	26.928523576397836	0.0
27	0.0	0.0	0.0	27.454573563514558	0.0
28	0.0	0.0	0.0	27.96701880958516	0.0
29	0.0	0.0	0.0	28.50894099458902	0.0
30	0.0	0.0	0.0	29.240505024478228	0.0
31	0.0	0.0	0.0	29.745529502705487	0.0
32	0.0	0.0	0.0	30.238186034527185	0.0
33	0.0	0.0	0.0	30.71682556042257	0.0
34	0.0	0.0	0.0	31.169698531306363	0.0
35	0.0	0.0	0.0	31.69286266426179	0.0
36	0.0	0.0	0.0	32.16923473331615	0.0
37	0.0	0.0	0.0	32.659005410976555	0.0
38	0.0	0.0	0.0	33.13682040711157	0.0
39	0.0	0.0	0.0	33.61257407884566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCG	30	2.1628985E-6	45.000004	1
CGGGTCA	30	2.1628985E-6	45.000004	6
AGATCGA	30	2.1628985E-6	45.000004	15
CGAGTAG	30	2.1628985E-6	45.000004	1
ACGTTAG	30	2.1628985E-6	45.000004	1
CGGCAGA	30	2.1628985E-6	45.000004	36
TAAACCG	30	2.1628985E-6	45.000004	1
ATTCGCG	30	2.1628985E-6	45.000004	1
TAACGGC	30	2.1628985E-6	45.000004	3
CACTGCG	30	2.1628985E-6	45.000004	1
CCTCGTC	30	2.1628985E-6	45.000004	36
CCCAACG	30	2.1628985E-6	45.000004	1
TCGCTAG	30	2.1628985E-6	45.000004	1
TCGTCCC	30	2.1628985E-6	45.000004	38
GCGATGT	30	2.1628985E-6	45.000004	9
AGTTCGT	30	2.1628985E-6	45.000004	30
CCGACCG	55	1.8189894E-12	45.000004	1
TATAGCG	30	2.1628985E-6	45.000004	1
TGCAACG	30	2.1628985E-6	45.000004	1
CGGAACG	45	3.8380676E-10	45.0	1
>>END_MODULE