FastQCFastQC Report
Sat 18 Jun 2016
SRR3553531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487960
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT57191.1720222969095826Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11440.2344454463480613No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC10690.21907533404377405No Hit
AATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT10590.21702598573653578No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC9460.193868349864743Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT9300.19058939257316174No Hit
AAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT8790.1801377162062464No Hit
AACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT6210.12726452987949832No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG6190.12685466021805067No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT5940.12173128944995493No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4990.1022624805311911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA207.029291E-445.00000440
CAGCGTG207.029291E-445.00000430
AACGTAG453.8380676E-1045.0000041
CGAACAG453.8380676E-1045.0000041
ACTGCGC207.029291E-445.00000420
GTCGACG207.029291E-445.00000413
TTTACGG207.029291E-445.0000042
AGATACT207.029291E-445.00000418
CGTGCCC207.029291E-445.00000441
CCTAGCG453.8380676E-1045.0000041
GAGTCCG207.029291E-445.0000049
GCGAAAG406.8012014E-945.0000041
GACGTCA207.029291E-445.00000440
CGTGACG207.029291E-445.00000437
GAACTAC406.8012014E-945.00000419
CGTTGAT207.029291E-445.00000425
TAGCGGA207.029291E-445.0000043
CCAATTA207.029291E-445.00000430
CGACCTA207.029291E-445.00000417
ACCGAGG207.029291E-445.0000042