Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553531_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 487960 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT | 5719 | 1.1720222969095826 | Illumina Single End Adapter 1 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1144 | 0.2344454463480613 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC | 1069 | 0.21907533404377405 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT | 1059 | 0.21702598573653578 | No Hit |
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC | 946 | 0.193868349864743 | Illumina Single End Adapter 1 (95% over 21bp) |
AATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT | 930 | 0.19058939257316174 | No Hit |
AAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT | 879 | 0.1801377162062464 | No Hit |
AACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT | 621 | 0.12726452987949832 | No Hit |
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG | 619 | 0.12685466021805067 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT | 594 | 0.12173128944995493 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 499 | 0.1022624805311911 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCCAA | 20 | 7.029291E-4 | 45.000004 | 40 |
CAGCGTG | 20 | 7.029291E-4 | 45.000004 | 30 |
AACGTAG | 45 | 3.8380676E-10 | 45.000004 | 1 |
CGAACAG | 45 | 3.8380676E-10 | 45.000004 | 1 |
ACTGCGC | 20 | 7.029291E-4 | 45.000004 | 20 |
GTCGACG | 20 | 7.029291E-4 | 45.000004 | 13 |
TTTACGG | 20 | 7.029291E-4 | 45.000004 | 2 |
AGATACT | 20 | 7.029291E-4 | 45.000004 | 18 |
CGTGCCC | 20 | 7.029291E-4 | 45.000004 | 41 |
CCTAGCG | 45 | 3.8380676E-10 | 45.000004 | 1 |
GAGTCCG | 20 | 7.029291E-4 | 45.000004 | 9 |
GCGAAAG | 40 | 6.8012014E-9 | 45.000004 | 1 |
GACGTCA | 20 | 7.029291E-4 | 45.000004 | 40 |
CGTGACG | 20 | 7.029291E-4 | 45.000004 | 37 |
GAACTAC | 40 | 6.8012014E-9 | 45.000004 | 19 |
CGTTGAT | 20 | 7.029291E-4 | 45.000004 | 25 |
TAGCGGA | 20 | 7.029291E-4 | 45.000004 | 3 |
CCAATTA | 20 | 7.029291E-4 | 45.000004 | 30 |
CGACCTA | 20 | 7.029291E-4 | 45.000004 | 17 |
ACCGAGG | 20 | 7.029291E-4 | 45.000004 | 2 |