##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487960
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.764933601114844	31.0	31.0	34.0	30.0	34.0
2	31.962017378473647	33.0	31.0	34.0	30.0	34.0
3	32.12671325518485	34.0	31.0	34.0	30.0	34.0
4	35.733404377407986	37.0	35.0	37.0	33.0	37.0
5	35.56135543897041	37.0	35.0	37.0	33.0	37.0
6	35.570122551028774	37.0	35.0	37.0	33.0	37.0
7	35.742485039757355	37.0	35.0	37.0	35.0	37.0
8	35.93782892040331	37.0	35.0	37.0	35.0	37.0
9	37.527481760800065	39.0	37.0	39.0	35.0	39.0
10	37.00775473399459	39.0	37.0	39.0	33.0	39.0
11	37.031719813099436	39.0	37.0	39.0	33.0	39.0
12	37.04106484138044	39.0	37.0	39.0	33.0	39.0
13	36.994431920649234	39.0	37.0	39.0	33.0	39.0
14	38.14962087056316	40.0	37.0	41.0	33.0	41.0
15	38.1407984261005	40.0	37.0	41.0	33.0	41.0
16	38.07595909500779	40.0	37.0	41.0	33.0	41.0
17	38.08455816050496	40.0	37.0	41.0	33.0	41.0
18	38.04431920649233	40.0	37.0	41.0	33.0	41.0
19	38.0486966144766	40.0	37.0	41.0	33.0	41.0
20	37.97116771866546	40.0	37.0	41.0	33.0	41.0
21	37.92961103369129	40.0	37.0	41.0	33.0	41.0
22	37.93500696778425	40.0	37.0	41.0	33.0	41.0
23	37.8335211902615	40.0	37.0	41.0	33.0	41.0
24	37.76708131814083	39.0	37.0	41.0	33.0	41.0
25	37.79654889745061	40.0	37.0	41.0	33.0	41.0
26	37.71313222395278	40.0	37.0	41.0	33.0	41.0
27	37.69069595868514	40.0	37.0	41.0	33.0	41.0
28	37.658367489138456	40.0	37.0	41.0	33.0	41.0
29	37.49931346831708	39.0	36.0	41.0	32.0	41.0
30	37.49302811705878	39.0	36.0	41.0	32.0	41.0
31	37.420235265185674	39.0	36.0	41.0	32.0	41.0
32	37.333256824329865	39.0	36.0	41.0	32.0	41.0
33	37.211009099106484	39.0	36.0	41.0	31.0	41.0
34	37.18358062136241	39.0	36.0	41.0	31.0	41.0
35	37.09275965243053	39.0	36.0	41.0	31.0	41.0
36	36.96242724813509	39.0	35.0	41.0	31.0	41.0
37	36.915843511763256	39.0	35.0	41.0	31.0	41.0
38	36.80135052053447	39.0	35.0	40.0	30.0	41.0
39	36.71904254447086	39.0	35.0	40.0	30.0	41.0
40	36.709529469628656	39.0	35.0	40.0	30.0	41.0
41	36.69587261250922	39.0	35.0	40.0	30.0	41.0
42	36.55267030084433	39.0	35.0	40.0	30.0	41.0
43	36.45555578326092	39.0	35.0	40.0	30.0	41.0
44	36.46462824821707	39.0	35.0	40.0	30.0	41.0
45	36.3432330518895	39.0	35.0	40.0	30.0	41.0
46	36.18625297155504	39.0	35.0	40.0	29.0	41.0
47	36.16202352651857	38.0	35.0	40.0	29.0	41.0
48	36.138843347815396	38.0	35.0	40.0	29.0	41.0
49	35.9683191245184	38.0	35.0	40.0	28.0	41.0
50	35.752541191900974	38.0	34.0	40.0	28.0	41.0
51	34.5424112632183	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	2.0
14	3.0
15	4.0
16	19.0
17	50.0
18	106.0
19	214.0
20	425.0
21	647.0
22	1020.0
23	1389.0
24	1737.0
25	2296.0
26	2839.0
27	3626.0
28	4835.0
29	6290.0
30	8373.0
31	11065.0
32	14732.0
33	19671.0
34	27797.0
35	35950.0
36	46071.0
37	66879.0
38	104040.0
39	127835.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.102877285023364	32.035207803918354	22.445897204688908	25.416017706369377
2	21.353594556930897	30.599229445036478	21.931510779572097	26.11566521846053
3	20.14611853430609	31.818591687843266	21.56201327977703	26.473276498073613
4	19.783588818755636	32.27375194688089	20.33076481678826	27.61189441757521
5	17.617017788343308	34.8915894745471	19.082096893187966	28.409295843921633
6	20.587343224854497	36.59746700549225	20.510287728502334	22.304902041150914
7	84.35056152143619	4.194606115255349	6.252356750553324	5.202475612755144
8	86.2443642921551	3.570989425362735	4.6671858349045	5.517460447577671
9	77.5532830559882	8.721206656283302	9.24870891056644	4.4768013771620625
10	27.655545536519387	44.779490122141155	12.848594147061235	14.71637019427822
11	19.67087466185753	25.9769243380605	36.41609968030167	17.93610131978031
12	23.396999754078205	23.073407656365276	35.38466267726863	18.144929912287893
13	24.75592261660792	25.369907369456513	30.47585867694073	19.398311336994837
14	18.874497909664726	28.878801541109926	31.835806213624068	20.410894335601277
15	18.1570210673006	30.504139683580622	30.837158783506847	20.501680465611933
16	19.35998852364948	29.72743667513731	28.560742683826547	22.35183211738667
17	18.473850315599638	28.731043528158047	31.006230018854	21.78887613738831
18	19.347282564144603	26.954258545782437	29.839331092712516	23.85912779736044
19	19.53356832527256	32.03172391179605	30.091605869333556	18.343101893597836
20	22.445077465366015	29.219198294942206	29.236822690384457	19.09890154930732
21	20.846790720550864	29.62906795638987	28.589843429789326	20.93429789326994
22	19.331912451840314	26.894622510041806	30.025411919009752	23.74805311910812
23	20.7971964915157	28.301295188130176	26.438642511681287	24.46286580867284
24	17.09914747110419	29.859209771292726	29.756742355930815	23.284900401672267
25	19.652430527092385	28.611566521846054	28.53471596032462	23.201286990736946
26	20.987580949258135	30.711328797442413	26.710591032051806	21.590499221247644
27	17.80391835396344	28.44433969997541	28.283260923026475	25.468481023034673
28	19.728666284121648	27.646938273628983	30.426264447905567	22.1981309943438
29	19.81965734896303	31.285761127961308	25.69452414132306	23.200057381752604
30	19.90040167226822	28.65378309697516	26.827198950733667	24.618616280022955
31	23.978194934010986	29.51922288712189	23.862611689482744	22.639970489384375
32	21.774120829576194	30.544921714894663	24.53602754324125	23.144929912287893
33	22.14730715632429	28.96487417001394	26.635175014345435	22.252643659316337
34	21.220386916960408	27.936716124272483	26.897696532502664	23.94520042626445
35	21.549717189933602	28.971022214935648	26.99483564226576	22.48442495286499
36	21.34785638167063	29.315927535043855	28.422821542749404	20.91339454053611
37	22.843060906631692	31.07119436019346	23.708090827116976	22.377653906057873
38	23.519140913189606	29.064267562914992	27.552668251496026	19.86392327239938
39	23.22546930076236	28.23407656365276	27.08111320600049	21.459340929584393
40	25.25616853840479	28.25723419952455	25.296950569718827	21.189646692351833
41	23.525084023280595	25.29387654725797	29.780924665956228	21.400114763505204
42	25.39654889745061	26.30912369866382	26.59029428641692	21.704033117468647
43	21.091482908435115	26.296212804328224	28.518526108697433	24.093778178539225
44	22.560250840232808	25.37503074022461	26.39724567587507	25.667472743667513
45	23.869374538896633	26.127346503811786	28.2172719075334	21.786007049758176
46	19.488277727682597	27.94614312648578	31.27940814820887	21.286170997622754
47	22.138494958603165	27.389949995901304	27.03295352078039	23.438601524715143
48	23.172186244774164	24.023280596770228	30.979998360521353	21.824534797934255
49	23.354373309287645	23.568940077055498	28.80727928518731	24.269407328469548
50	21.820845970981228	24.122878924502007	26.570210673005985	27.486064431510783
51	21.299286826789082	23.021764079022873	31.865521764079023	23.813427330109025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1498.0
1	1197.5
2	897.0
3	628.5
4	360.0
5	321.5
6	283.0
7	315.0
8	347.0
9	410.5
10	474.0
11	578.0
12	682.0
13	774.5
14	867.0
15	921.5
16	976.0
17	1371.0
18	1766.0
19	1741.0
20	1716.0
21	1801.0
22	1886.0
23	2127.0
24	2368.0
25	2783.5
26	4180.0
27	5161.0
28	5908.5
29	6656.0
30	8222.5
31	9789.0
32	11557.5
33	13326.0
34	14580.0
35	15834.0
36	18005.5
37	20177.0
38	21154.5
39	22132.0
40	22852.5
41	23573.0
42	25813.0
43	28053.0
44	33911.5
45	39770.0
46	45309.0
47	50848.0
48	54949.0
49	59050.0
50	54415.0
51	49780.0
52	42527.0
53	35274.0
54	29966.0
55	24658.0
56	21150.0
57	17642.0
58	15435.0
59	13228.0
60	12419.0
61	11610.0
62	10117.5
63	8625.0
64	7222.0
65	5819.0
66	4765.0
67	3711.0
68	3041.0
69	2371.0
70	1846.5
71	1322.0
72	1099.5
73	877.0
74	743.5
75	471.5
76	333.0
77	227.5
78	122.0
79	114.5
80	107.0
81	88.0
82	69.0
83	54.0
84	39.0
85	49.0
86	59.0
87	33.0
88	7.0
89	5.0
90	3.0
91	4.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.89604927617508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12551612919479	21.86164033544143
2	11.648538214661125	6.964905449218349
3	4.506431827875688	4.041735239576859
4	2.2218063537060675	2.656929289300619
5	1.274244033209132	1.9047431203346141
6	0.8133653806947465	1.4589846900471026
7	0.6122144341076808	1.2811955022768207
8	0.465622828004485	1.1136226408107261
9	0.36836785813269907	0.9911469274644819
>10	3.7833349741736093	26.579925636719416
>50	0.9905672964544431	20.438221119915344
>100	0.183106949865797	7.912239962516339
>500	0.004130231951860083	0.944397981324615
>1k	0.0020651159759300414	0.6733646099137373
>5k	6.883719919766805E-4	1.1769474951395673
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	5719	1.1720222969095826	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1144	0.2344454463480613	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	1069	0.21907533404377405	No Hit
AATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	1059	0.21702598573653578	No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	946	0.193868349864743	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGT	930	0.19058939257316174	No Hit
AAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	879	0.1801377162062464	No Hit
AACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	621	0.12726452987949832	No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	619	0.12685466021805067	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT	594	0.12173128944995493	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	499	0.1022624805311911	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3072792851873105	0.0
2	0.0	0.0	0.0	1.772891220591852	0.0
3	0.0	0.0	0.0	2.306336584965981	0.0
4	0.0	0.0	0.0	4.5606197229281085	0.0
5	0.0	0.0	0.0	4.859004836462005	0.0
6	0.0	0.0	0.0	6.531068120337733	0.0
7	0.0	0.0	0.0	7.7940814820886954	0.0
8	0.0	0.0	0.0	8.961185343060906	0.0
9	0.0	0.0	0.0	10.757644069185998	0.0
10	0.0	0.0	0.0	13.286130010656612	0.0
11	0.0	0.0	0.0	15.518690056562013	0.0
12	0.0	0.0	0.0	16.646856299696697	0.0
13	0.0	0.0	0.0	17.080293466677595	0.0
14	0.0	0.0	0.0	17.5438560537749	0.0
15	0.0	0.0	0.0	17.976473481432905	0.0
16	0.0	0.0	0.0	18.81424706943192	0.0
17	0.0	0.0	0.0	20.051233707680957	0.0
18	0.0	0.0	0.0	21.54213460119682	0.0
19	0.0	0.0	0.0	22.216984998770393	0.0
20	0.0	0.0	0.0	22.92544470858267	0.0
21	0.0	0.0	0.0	23.661365685711942	0.0
22	0.0	0.0	0.0	24.48684318386753	0.0
23	0.0	0.0	0.0	25.271333715878352	0.0
24	0.0	0.0	0.0	25.824452824001966	0.0
25	0.0	0.0	0.0	26.276948930240184	0.0
26	0.0	0.0	0.0	26.7294450364784	0.0
27	0.0	0.0	0.0	27.238503155996394	0.0
28	0.0	0.0	0.0	27.740593491269777	0.0
29	0.0	0.0	0.0	28.27977703090417	0.0
30	0.0	0.0	0.0	28.987212066562833	0.0
31	0.0	0.0	0.0	29.497909664726617	0.0
32	0.0	0.0	0.0	29.976227559636037	0.0
33	0.0	0.0	0.0	30.44306910402492	0.0
34	0.0	0.0	0.0	30.911755061890318	0.0
35	0.0	0.0	0.0	31.425321747684237	0.0
36	0.0	0.0	0.0	31.88560537748996	0.0
37	0.0	0.0	0.0	32.356340683662594	0.0
38	0.0	0.0	0.0	32.82195261906713	0.0
39	0.0	0.0	0.0	33.32568243298631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	20	7.029291E-4	45.000004	40
CAGCGTG	20	7.029291E-4	45.000004	30
AACGTAG	45	3.8380676E-10	45.000004	1
CGAACAG	45	3.8380676E-10	45.000004	1
ACTGCGC	20	7.029291E-4	45.000004	20
GTCGACG	20	7.029291E-4	45.000004	13
TTTACGG	20	7.029291E-4	45.000004	2
AGATACT	20	7.029291E-4	45.000004	18
CGTGCCC	20	7.029291E-4	45.000004	41
CCTAGCG	45	3.8380676E-10	45.000004	1
GAGTCCG	20	7.029291E-4	45.000004	9
GCGAAAG	40	6.8012014E-9	45.000004	1
GACGTCA	20	7.029291E-4	45.000004	40
CGTGACG	20	7.029291E-4	45.000004	37
GAACTAC	40	6.8012014E-9	45.000004	19
CGTTGAT	20	7.029291E-4	45.000004	25
TAGCGGA	20	7.029291E-4	45.000004	3
CCAATTA	20	7.029291E-4	45.000004	30
CGACCTA	20	7.029291E-4	45.000004	17
ACCGAGG	20	7.029291E-4	45.000004	2
>>END_MODULE