FastQCFastQC Report
Sat 18 Jun 2016
SRR3553529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285393
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT62170.48366530703061245No Hit
AATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT17990.13995719597041528No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA17370.13513376842724364No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC14790.11506208607017464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAC253.8910635E-545.00000428
CGATCTA253.8910635E-545.00000430
TCGACTA253.8910635E-545.00000417
AGTCGAC253.8910635E-545.00000415
TCGCACG453.8562575E-1045.01
ACGCATG453.8562575E-1045.01
GACACGC207.0335995E-445.09
TACGAGC207.0335995E-445.021
TGCGACG406.8157533E-945.01
CGTACCG406.8157533E-945.01
TTCGCAC207.0335995E-445.017
AGTCGAA207.0335995E-445.027
CGCAATG1400.041.7857171
AACCGCG650.041.538461
CTTACCG650.041.538461
CGCACTG1050.040.7142831
ATTCGCG1000.040.5000041
CGATTAG501.0822987E-940.5000041
CCGCTCG501.0822987E-940.5000041
CTAACCG1000.040.5000041