##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285393
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.898266133392667	33.0	31.0	34.0	30.0	34.0
2	32.09245421439202	34.0	31.0	34.0	30.0	34.0
3	32.24393317841314	34.0	31.0	34.0	30.0	34.0
4	35.825922499966936	37.0	35.0	37.0	35.0	37.0
5	35.6629521088103	37.0	35.0	37.0	33.0	37.0
6	35.66525490647607	37.0	35.0	37.0	33.0	37.0
7	35.842094207763694	37.0	35.0	37.0	35.0	37.0
8	36.00214564728453	37.0	35.0	37.0	35.0	37.0
9	37.58673261796198	39.0	37.0	39.0	35.0	39.0
10	37.11079490863884	39.0	37.0	39.0	33.0	39.0
11	37.099690911651145	39.0	37.0	39.0	33.0	39.0
12	37.13243809480836	39.0	37.0	39.0	33.0	39.0
13	37.09412063081096	39.0	37.0	39.0	33.0	39.0
14	38.3145963919206	40.0	38.0	41.0	34.0	41.0
15	38.28053599171615	40.0	38.0	41.0	33.0	41.0
16	38.23848737312246	40.0	38.0	41.0	33.0	41.0
17	38.222584065729315	40.0	38.0	41.0	33.0	41.0
18	38.18739404991314	40.0	37.0	41.0	33.0	41.0
19	38.2009805561412	40.0	37.0	41.0	34.0	41.0
20	38.13668115510198	40.0	37.0	41.0	33.0	41.0
21	38.1088787631487	40.0	37.0	41.0	33.0	41.0
22	38.09873478383654	40.0	37.0	41.0	33.0	41.0
23	38.0064758404628	40.0	37.0	41.0	33.0	41.0
24	37.946936851219824	40.0	37.0	41.0	33.0	41.0
25	37.95644600522953	40.0	37.0	41.0	33.0	41.0
26	37.8547564830367	40.0	37.0	41.0	33.0	41.0
27	37.798030641212456	40.0	37.0	41.0	33.0	41.0
28	37.78172201031124	40.0	37.0	41.0	33.0	41.0
29	37.568751346864346	40.0	37.0	41.0	32.0	41.0
30	37.6208692594405	40.0	37.0	41.0	32.0	41.0
31	37.52481147789042	40.0	37.0	41.0	32.0	41.0
32	37.46393982229559	40.0	37.0	41.0	32.0	41.0
33	37.447272546217384	40.0	36.0	41.0	32.0	41.0
34	37.31501960878891	39.0	36.0	41.0	31.0	41.0
35	37.22611605944641	39.0	36.0	41.0	31.0	41.0
36	37.17880056916445	39.0	36.0	41.0	31.0	41.0
37	37.0763999803951	39.0	36.0	41.0	31.0	41.0
38	37.06408468071633	39.0	36.0	41.0	31.0	41.0
39	37.00473318276978	39.0	36.0	41.0	31.0	41.0
40	36.956274851348965	39.0	35.0	41.0	31.0	41.0
41	36.9674216368068	39.0	35.0	41.0	31.0	41.0
42	36.8733422385216	39.0	35.0	41.0	31.0	41.0
43	36.80143504749131	39.0	35.0	41.0	31.0	41.0
44	36.717940738746826	39.0	35.0	41.0	30.0	41.0
45	36.51715389767954	39.0	35.0	40.0	30.0	41.0
46	36.37610909659536	39.0	35.0	40.0	30.0	41.0
47	36.28534385981563	39.0	35.0	40.0	29.0	41.0
48	36.25392000734406	39.0	35.0	40.0	29.0	41.0
49	36.12090543514707	39.0	35.0	40.0	29.0	41.0
50	35.94748065377671	38.0	35.0	40.0	28.0	41.0
51	34.74272148673597	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	10.0
12	4.0
13	23.0
14	23.0
15	36.0
16	58.0
17	126.0
18	231.0
19	422.0
20	855.0
21	1332.0
22	2112.0
23	3057.0
24	4183.0
25	5389.0
26	7324.0
27	9638.0
28	12503.0
29	16393.0
30	21773.0
31	28232.0
32	36642.0
33	48519.0
34	69333.0
35	86547.0
36	112477.0
37	164894.0
38	275501.0
39	377621.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.736220751163263	32.980886001401906	20.62412040519903	26.658772842235802
2	21.838690579457023	30.373590022662327	21.570056784189738	26.217662613690912
3	20.809199987863632	30.381914325035225	21.280495537162565	27.52839014993858
4	20.36754517878968	30.59601227017729	19.899205923791403	29.13723662724163
5	18.43366192285161	33.063273255728014	18.068481779502456	30.434583041917918
6	21.10879707606934	35.03216525996329	19.672660423699213	24.18637724026815
7	87.21122644980952	3.7248530216050657	4.81502544358029	4.24889508500513
8	88.61321012328526	3.185095920080473	3.8822367945056495	4.319457162128625
9	79.6134722999114	8.272333830976207	7.764706980666613	4.3494868884457905
10	29.53104614697606	43.8713296244806	12.666554120023992	13.93107010851934
11	20.825770795390984	26.15161277523683	34.496764802671244	18.52585162670094
12	25.165377437095117	22.813723118143635	33.39468940627497	18.626210038486285
13	24.557080986126422	23.8075825836923	30.5734510768302	21.061885353351077
14	19.566156031657243	27.50131671792207	31.155763256840512	21.776763993580172
15	18.169540366253745	30.45504371036718	30.942209892227513	20.433206031151563
16	20.867392307255447	28.05990074630872	30.070569856845335	21.002137089590498
17	19.617502195826493	28.094364914076863	30.79991877970395	21.488214110392697
18	20.437562675384104	27.37466284630459	29.354835447213418	22.832939031097883
19	20.925662423865695	30.646658259380594	28.472459395686766	19.95521992106694
20	23.160387523504486	28.16648293556912	28.477827403759004	20.19530213716739
21	22.14622298394343	29.263190323893156	27.21113309314739	21.37945359901602
22	20.491242756106498	27.52574504451168	28.534463778782055	23.448548420599767
23	20.704562729064186	28.86486856548931	26.882284250808897	23.548284454637606
24	18.6138402807546	28.784504038842595	29.06994203329254	23.531713647110262
25	21.195463177409554	29.392567098155975	25.680006037064153	23.73196368737032
26	22.32741270568612	29.629070642208262	26.426392550760742	21.61712410134488
27	20.37859238380791	28.481561670244044	27.556552743013224	23.583293202934822
28	22.2090053392231	28.27547683860111	28.509024088352746	21.00649373382304
29	22.520272010194546	27.42554222716321	27.733697009397122	22.320488753245115
30	24.074582637372384	26.325022775135697	27.81320576664102	21.7871888208509
31	23.16723367872705	27.024030782803393	26.541843622923107	23.266891915546452
32	24.204348397727387	25.9707342423679	28.204603572603865	21.620313787300848
33	23.72628449042433	26.581831393200368	28.315386811659938	21.376497304715368
34	22.54065488142537	25.283940397995007	28.99510110915494	23.180303611424677
35	23.26945922375491	24.61799620816357	30.920504468283237	21.19204009979827
36	22.74930702127676	27.27368205677174	29.155129987482425	20.82188093446907
37	23.321972346200734	26.889363797686777	28.593356273139808	21.195307582972678
38	22.214995725042847	25.208632690546786	31.493091995988777	21.083279588421597
39	21.213356537650352	24.15136849197094	30.964459896700852	23.670815073677858
40	22.17617491304216	25.23866241686395	30.458311193541586	22.126851476552307
41	19.393990787253394	24.59551281203492	33.051448078525404	22.959048322186288
42	22.9212388740253	25.906006956627277	29.47184246374455	21.700911705602877
43	21.711336532873602	26.00706554337856	29.674971001086824	22.606626922661007
44	24.074816029027698	25.282617845281557	27.585104322180065	23.057461803510677
45	23.78323205432113	24.53171909291555	29.29788788331662	22.387160969446697
46	20.43911861975287	26.065724646081005	30.689368932303196	22.80578780186293
47	21.636184419862253	25.68817474500017	29.71083551878686	22.96480531635072
48	21.655555927253378	23.38755540134418	31.693342036248833	23.263546635153606
49	21.41905238320109	25.154563623732194	29.712858246466254	23.713525746600457
50	20.06436941853581	24.28284579113158	29.75401297502009	25.898771815312514
51	20.050365919216926	23.194151516306686	31.72025987382847	25.035222690647917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1067.0
1	833.5
2	600.0
3	458.5
4	317.0
5	355.5
6	394.0
7	431.5
8	469.0
9	600.0
10	731.0
11	931.5
12	1132.0
13	1345.5
14	1559.0
15	1624.0
16	1689.0
17	1952.5
18	2216.0
19	2664.0
20	3112.0
21	3782.5
22	4453.0
23	5626.0
24	6799.0
25	8245.0
26	11966.0
27	14241.0
28	18034.0
29	21827.0
30	24326.5
31	26826.0
32	32497.5
33	38169.0
34	41358.5
35	44548.0
36	49065.5
37	53583.0
38	57445.5
39	61308.0
40	63161.0
41	65014.0
42	70156.5
43	75299.0
44	84257.0
45	93215.0
46	104238.0
47	115261.0
48	125913.0
49	136565.0
50	128146.5
51	119728.0
52	107737.0
53	95746.0
54	83990.0
55	72234.0
56	62818.5
57	53403.0
58	49749.5
59	46096.0
60	43715.0
61	41334.0
62	36554.0
63	31774.0
64	25041.0
65	18308.0
66	14604.0
67	10900.0
68	8420.5
69	5941.0
70	4794.5
71	3648.0
72	3047.5
73	2447.0
74	1985.5
75	1328.5
76	1133.0
77	766.5
78	400.0
79	374.0
80	348.0
81	240.0
82	132.0
83	99.5
84	67.0
85	86.5
86	106.0
87	61.0
88	16.0
89	13.0
90	10.0
91	6.5
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1285393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.170695688184992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91750920684484	20.823076578946388
2	10.344327050930243	5.828137789016319
3	3.738317668226846	3.159330282521513
4	1.92193979367528	2.1656952423455746
5	1.216192782933139	1.7130498393088143
6	0.8616456261176816	1.4563894034650102
7	0.5991857789449869	1.1815636167543115
8	0.5138607460787308	1.15806517631101
9	0.4649890621758312	1.1789158832000883
>10	5.255569392027576	34.05666574943189
>50	1.001656221381266	19.31394639953949
>100	0.16090921561630983	6.442556253336501
>500	0.002227117169876344	0.45015548239116865
>1k	0.001391948231172715	0.5848884299082467
>5k	2.78389646234543E-4	0.48756387352367525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	6217	0.48366530703061245	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	1799	0.13995719597041528	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1737	0.13513376842724364	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1479	0.11506208607017464	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5559443687650391E-4	0.0	0.0	0.567841897380801	0.0
2	1.5559443687650391E-4	0.0	0.0	0.8311076845758457	0.0
3	1.5559443687650391E-4	0.0	0.0	1.0989635076587472	0.0
4	1.5559443687650391E-4	0.0	0.0	2.370403448595099	0.0
5	1.5559443687650391E-4	0.0	0.0	2.604106292783608	0.0
6	1.5559443687650391E-4	0.0	0.0	3.982439611854118	0.0
7	1.5559443687650391E-4	0.0	0.0	4.98057792441689	0.0
8	1.5559443687650391E-4	0.0	0.0	5.9214574842091094	0.0
9	1.5559443687650391E-4	0.0	0.0	7.186907039325716	0.0
10	1.5559443687650391E-4	0.0	0.0	9.05100619032467	0.0
11	1.5559443687650391E-4	0.0	0.0	10.696106171419947	0.0
12	1.5559443687650391E-4	0.0	0.0	11.545885188420973	0.0
13	1.5559443687650391E-4	0.0	0.0	11.923357292283372	0.0
14	1.5559443687650391E-4	0.0	0.0	12.316233245396544	0.0
15	1.5559443687650391E-4	0.0	0.0	12.620887152800739	0.0
16	1.5559443687650391E-4	0.0	0.0	13.207555977043597	0.0
17	1.5559443687650391E-4	0.0	0.0	14.034773800697529	0.0
18	1.5559443687650391E-4	0.0	0.0	14.937065940144375	0.0
19	1.5559443687650391E-4	0.0	0.0	15.416841386253076	0.0
20	1.5559443687650391E-4	0.0	0.0	15.95294201851107	0.0
21	1.5559443687650391E-4	0.0	0.0	16.488653664676875	0.0
22	1.5559443687650391E-4	0.0	0.0	17.15273072126579	0.0
23	1.5559443687650391E-4	0.0	0.0	17.767095355272666	0.0
24	1.5559443687650391E-4	0.0	0.0	18.214118172418864	0.0
25	1.5559443687650391E-4	0.0	0.0	18.604893600634202	0.0
26	1.5559443687650391E-4	0.0	0.0	18.964861330347993	0.0
27	1.5559443687650391E-4	0.0	0.0	19.38745582090458	0.0
28	1.5559443687650391E-4	0.0	0.0	19.809505730932095	0.0
29	1.5559443687650391E-4	0.0	0.0	20.245714734715374	0.0
30	1.5559443687650391E-4	0.0	0.0	20.88054003717151	0.0
31	1.5559443687650391E-4	0.0	0.0	21.318771768634186	0.0
32	1.5559443687650391E-4	0.0	0.0	21.764160844193178	0.0
33	2.3339165531475589E-4	0.0	0.0	22.187922293026336	0.0
34	2.3339165531475589E-4	0.0	0.0	22.58025366561044	0.0
35	2.3339165531475589E-4	0.0	0.0	23.036067568440156	0.0
36	2.3339165531475589E-4	0.0	0.0	23.467064158588073	0.0
37	2.3339165531475589E-4	0.0	0.0	23.891603579605615	0.0
38	2.3339165531475589E-4	0.0	0.0	24.3141980701622	0.0
39	2.3339165531475589E-4	0.0	0.0	24.724811789079293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGAAC	25	3.8910635E-5	45.000004	28
CGATCTA	25	3.8910635E-5	45.000004	30
TCGACTA	25	3.8910635E-5	45.000004	17
AGTCGAC	25	3.8910635E-5	45.000004	15
TCGCACG	45	3.8562575E-10	45.0	1
ACGCATG	45	3.8562575E-10	45.0	1
GACACGC	20	7.0335995E-4	45.0	9
TACGAGC	20	7.0335995E-4	45.0	21
TGCGACG	40	6.8157533E-9	45.0	1
CGTACCG	40	6.8157533E-9	45.0	1
TTCGCAC	20	7.0335995E-4	45.0	17
AGTCGAA	20	7.0335995E-4	45.0	27
CGCAATG	140	0.0	41.785717	1
AACCGCG	65	0.0	41.53846	1
CTTACCG	65	0.0	41.53846	1
CGCACTG	105	0.0	40.714283	1
ATTCGCG	100	0.0	40.500004	1
CGATTAG	50	1.0822987E-9	40.500004	1
CCGCTCG	50	1.0822987E-9	40.500004	1
CTAACCG	100	0.0	40.500004	1
>>END_MODULE