FastQCFastQC Report
Sat 18 Jun 2016
SRR3553526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197040
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT35420.2958965448105326Illumina Paired End PCR Primer 2 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT13760.11495021051928088RNA PCR Primer, Index 23 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG13180.1101049254828577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG650.045.0000041
TATCACG253.890904E-545.01
TCGCATA207.0334045E-445.020
CGTATAG453.8562575E-1045.01
CGCATAA207.0334045E-445.021
ATTAACG351.2119926E-745.01
CATTCCG351.2119926E-745.01
GTAATCG207.0334045E-445.034
AATTGCG253.890904E-545.01
CGTAATC207.0334045E-445.033
TATAGCG351.2119926E-745.01
CGCTTAG253.890904E-545.01
CGCTAAG850.042.352941
TACGGGT1900.041.447374
TCACACG1100.040.909091
CGAATAG556.184564E-1140.909091
CAGTACG451.9284926E-840.01
AATCCCG403.45839E-739.3751
CGTAGCG403.45839E-739.3751
TACGACG403.45839E-739.3751