Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553526_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1197040 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 51 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT | 3542 | 0.2958965448105326 | Illumina Paired End PCR Primer 2 (95% over 23bp) |
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT | 1376 | 0.11495021051928088 | RNA PCR Primer, Index 23 (95% over 21bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1318 | 0.1101049254828577 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCATAG | 65 | 0.0 | 45.000004 | 1 |
TATCACG | 25 | 3.890904E-5 | 45.0 | 1 |
TCGCATA | 20 | 7.0334045E-4 | 45.0 | 20 |
CGTATAG | 45 | 3.8562575E-10 | 45.0 | 1 |
CGCATAA | 20 | 7.0334045E-4 | 45.0 | 21 |
ATTAACG | 35 | 1.2119926E-7 | 45.0 | 1 |
CATTCCG | 35 | 1.2119926E-7 | 45.0 | 1 |
GTAATCG | 20 | 7.0334045E-4 | 45.0 | 34 |
AATTGCG | 25 | 3.890904E-5 | 45.0 | 1 |
CGTAATC | 20 | 7.0334045E-4 | 45.0 | 33 |
TATAGCG | 35 | 1.2119926E-7 | 45.0 | 1 |
CGCTTAG | 25 | 3.890904E-5 | 45.0 | 1 |
CGCTAAG | 85 | 0.0 | 42.35294 | 1 |
TACGGGT | 190 | 0.0 | 41.44737 | 4 |
TCACACG | 110 | 0.0 | 40.90909 | 1 |
CGAATAG | 55 | 6.184564E-11 | 40.90909 | 1 |
CAGTACG | 45 | 1.9284926E-8 | 40.0 | 1 |
AATCCCG | 40 | 3.45839E-7 | 39.375 | 1 |
CGTAGCG | 40 | 3.45839E-7 | 39.375 | 1 |
TACGACG | 40 | 3.45839E-7 | 39.375 | 1 |