##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950390
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04880733172698	33.0	31.0	34.0	30.0	34.0
2	32.25733540967392	34.0	31.0	34.0	30.0	34.0
3	32.37857721566936	34.0	31.0	34.0	30.0	34.0
4	35.965239533244244	37.0	35.0	37.0	35.0	37.0
5	35.78097938740938	37.0	35.0	37.0	35.0	37.0
6	35.780133418912236	37.0	35.0	37.0	35.0	37.0
7	35.83511190142994	37.0	35.0	37.0	35.0	37.0
8	35.985730068708634	37.0	35.0	37.0	35.0	37.0
9	37.618072580730015	39.0	37.0	39.0	35.0	39.0
10	37.18356358968423	39.0	37.0	39.0	34.0	39.0
11	37.1470301665632	39.0	37.0	39.0	34.0	39.0
12	37.06599606477341	39.0	37.0	39.0	33.0	39.0
13	37.03990572291375	39.0	37.0	39.0	33.0	39.0
14	38.2635539094477	40.0	38.0	41.0	33.0	41.0
15	38.27276170835131	40.0	38.0	41.0	33.0	41.0
16	38.20733909237261	40.0	37.0	41.0	33.0	41.0
17	38.23462578520397	40.0	37.0	41.0	33.0	41.0
18	38.19682446153684	40.0	37.0	41.0	33.0	41.0
19	38.19301444670083	40.0	37.0	41.0	34.0	41.0
20	38.12435842127969	40.0	37.0	41.0	33.0	41.0
21	38.07741769168447	40.0	37.0	41.0	33.0	41.0
22	38.05833289491682	40.0	37.0	41.0	33.0	41.0
23	37.968415071707405	40.0	37.0	41.0	33.0	41.0
24	37.857316470080704	40.0	37.0	41.0	33.0	41.0
25	37.846109491892804	40.0	37.0	41.0	33.0	41.0
26	37.77866244383885	40.0	37.0	41.0	33.0	41.0
27	37.72133229516304	40.0	37.0	41.0	33.0	41.0
28	37.670387946001114	40.0	37.0	41.0	33.0	41.0
29	37.54095687033744	40.0	36.0	41.0	32.0	41.0
30	37.54289502204358	40.0	36.0	41.0	32.0	41.0
31	37.48126663790654	40.0	36.0	41.0	32.0	41.0
32	37.42621555361483	40.0	36.0	41.0	32.0	41.0
33	37.34905249423921	39.0	36.0	41.0	32.0	41.0
34	37.294471743179116	39.0	36.0	41.0	32.0	41.0
35	37.213907974620945	39.0	36.0	41.0	31.0	41.0
36	37.139531139847854	39.0	35.0	41.0	31.0	41.0
37	37.065336335609594	39.0	35.0	41.0	31.0	41.0
38	37.082320941929105	39.0	35.0	41.0	31.0	41.0
39	37.027044686918	39.0	35.0	41.0	31.0	41.0
40	36.90921726870022	39.0	35.0	41.0	31.0	41.0
41	36.79944759519776	39.0	35.0	41.0	31.0	41.0
42	36.72071149738528	39.0	35.0	41.0	31.0	41.0
43	36.64505413567062	39.0	35.0	40.0	31.0	41.0
44	36.52973095255632	39.0	35.0	40.0	30.0	41.0
45	36.379075958290805	38.0	35.0	40.0	30.0	41.0
46	36.26750071023474	38.0	35.0	40.0	30.0	41.0
47	36.16423047380549	38.0	35.0	40.0	30.0	41.0
48	36.08012184471638	38.0	35.0	40.0	30.0	41.0
49	35.9036606024895	38.0	35.0	40.0	29.0	41.0
50	35.761211713086205	38.0	34.0	40.0	29.0	41.0
51	34.53411862498553	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	3.0
12	5.0
13	11.0
14	22.0
15	38.0
16	75.0
17	126.0
18	230.0
19	429.0
20	724.0
21	1180.0
22	1677.0
23	2448.0
24	3251.0
25	4256.0
26	5426.0
27	6959.0
28	9014.0
29	11810.0
30	15384.0
31	19926.0
32	26419.0
33	35775.0
34	55707.0
35	68917.0
36	83770.0
37	123223.0
38	199487.0
39	273991.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.23495617588569	31.396374120098063	21.66226496490914	26.706404739107104
2	23.932806532055263	28.983154284030764	20.358905291511906	26.725133892402063
3	20.801039573227833	30.380159723902818	21.154683866623174	27.664116836246173
4	20.99043550542409	31.10428350466651	19.574069592483088	28.33121139742632
5	19.007354875366957	33.7874977640758	17.407380128157914	29.79776723239933
6	21.02578941276739	36.92599880049243	18.677700733383137	23.370511053357042
7	87.16474289502204	4.118730205494586	4.356632540325551	4.35989435915782
8	88.52544744788982	3.5390734330117106	3.830111848819958	4.105367270278517
9	80.8806910847126	7.585096644535401	7.0574185334441655	4.476793737307842
10	36.11759382990141	36.321615336861704	12.174475741537684	15.386315091699196
11	25.85685876324456	27.546165258472836	27.41442986563411	19.18254611264849
12	28.081313986889594	22.86829617314997	28.96894958911605	20.08144025084439
13	26.699249781668577	23.245509738107515	27.442628815538882	22.612611664685023
14	21.491913845894846	25.778785551194773	28.849524931870075	23.87977567104031
15	20.674986058354992	28.297646229442652	28.884773619251042	22.142594092951313
16	23.024337377287218	25.528362040846392	28.814276244489108	22.633024337377286
17	22.603667967886867	26.13095676511748	28.3355254158819	22.929849851113755
18	23.353254979534718	26.372331358705374	26.994181336083084	23.280232325676828
19	23.657130230747377	29.754521827881184	25.502793589999897	21.08555435137154
20	26.38053851576721	27.14632940161407	25.2631025158093	21.210029566809414
21	25.361588400551355	27.832153116089188	24.796662422794853	22.00959606056461
22	23.33526236597607	25.97512600090489	25.073496143688377	25.61611548943066
23	23.582108397605197	27.496396216290155	24.613895348225466	24.307600037879187
24	21.94215006471028	27.2982670272204	26.793842527804372	23.965740380264943
25	23.93354307179158	27.30300192552531	24.111470028093727	24.651984974589382
26	25.043508454423975	27.28669283136397	24.756994496995972	22.91280421721609
27	22.714254148297012	26.857921484864107	25.06507854670188	25.362745820136993
28	23.68932753922074	27.817527541325138	25.54772251391534	22.94542240553878
29	23.49793242774019	27.51302097033849	24.21637433053799	24.77267227138333
30	24.465219541451404	26.511221708982628	24.439440650680247	24.58411809888572
31	24.744999421290206	27.42642494133987	22.594934711013373	25.23364092635655
32	24.650091015267417	27.862772125127577	23.47089089742106	24.016245962183945
33	24.38662022958996	27.18946958616989	24.788665705657678	23.635244478582475
34	22.93447952945633	27.408432327781227	24.32285693241722	25.334231210345227
35	23.349361840928463	27.02848304380307	25.515525205441975	24.106629909826495
36	23.485937352034426	30.23443007607403	24.278769768200423	22.000862803691117
37	24.12746346236808	29.68844369153716	23.72131440776944	22.46277843832532
38	24.73752880396469	26.095287197887185	26.003114510885005	23.164069487263124
39	23.69669293658393	25.236797525226486	26.078136344027186	24.988373194162396
40	24.637043739938342	25.93966687359926	26.67494397037006	22.74834541609234
41	22.383232146802893	26.589189701070087	27.506812992560945	23.520765159566075
42	23.21383852944581	28.232304632834943	24.719010090594388	23.834846747124864
43	22.309472953208683	26.58771662159745	26.857184945127788	24.245625480066078
44	24.1130483275287	25.32255179452646	24.941234651037995	25.623165226906846
45	24.002146487231556	24.935237113185114	26.978819221582718	24.08379717800061
46	22.200570292195835	25.983964477740717	27.292900809141514	24.522564420921938
47	22.735719020612592	27.24997106451036	26.324666715769318	23.689643199107735
48	22.67584886204611	25.250897000178874	27.517019328907082	24.556234808867938
49	22.77086248803123	25.97512600090489	26.244699544397566	25.009311966666314
50	22.240974757731035	24.370521575353276	26.969770304822234	26.418733362093455
51	22.2125653679016	24.014457222824316	28.007344353370723	25.765633055903365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	654.0
1	608.5
2	563.0
3	440.5
4	318.0
5	313.5
6	309.0
7	350.0
8	391.0
9	453.0
10	515.0
11	751.5
12	988.0
13	959.0
14	930.0
15	986.5
16	1043.0
17	1075.5
18	1108.0
19	1247.0
20	1386.0
21	1947.0
22	2508.0
23	3087.5
24	3667.0
25	4454.0
26	5724.5
27	6208.0
28	8496.0
29	10784.0
30	13043.5
31	15303.0
32	17838.0
33	20373.0
34	22594.0
35	24815.0
36	27996.0
37	31177.0
38	32999.5
39	34822.0
40	37792.0
41	40762.0
42	44204.0
43	47646.0
44	53460.0
45	59274.0
46	66024.0
47	72774.0
48	79682.5
49	86591.0
50	83789.0
51	80987.0
52	75050.0
53	69113.0
54	62980.5
55	56848.0
56	53085.0
57	49322.0
58	49234.0
59	49146.0
60	47160.5
61	45175.0
62	42138.0
63	39101.0
64	33807.5
65	28514.0
66	23523.5
67	18533.0
68	16315.5
69	14098.0
70	12322.5
71	10547.0
72	8660.0
73	6773.0
74	6138.0
75	4242.0
76	2981.0
77	2369.0
78	1757.0
79	1387.0
80	1017.0
81	707.0
82	397.0
83	301.0
84	205.0
85	160.5
86	116.0
87	72.5
88	29.0
89	27.5
90	26.0
91	26.5
92	27.0
93	24.0
94	21.0
95	12.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	950390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.273100486846605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28986633669113	19.51825123418289
2	9.639709147209519	5.065300941772202
3	3.4033254016873538	2.6824773080390836
4	1.6951419496725457	1.781465391328635
5	1.113946476774066	1.463341386062689
6	0.8183166566124904	1.2899829449544187
7	0.6186529178773058	1.137775119450992
8	0.5042897835126501	1.0599404925374383
9	0.4327380299209934	1.0232432770134865
>10	6.1495973433092415	39.045386602689675
>50	1.2498317883328371	21.730276374325655
>100	0.08176469611989032	3.373010431337075
>500	0.0016111270171406957	0.2733414677838886
>1k	0.0012083452628555218	0.5562070285219197
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	3220	0.3388082787066362	Illumina Single End Adapter 1 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1022	0.10753480150254106	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1014	0.10669304180389104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40688559433495725	0.0
2	0.0	0.0	0.0	0.5711339555340439	0.0
3	0.0	0.0	0.0	0.7481139321752123	0.0
4	0.0	0.0	0.0	1.5604120413724891	0.0
5	0.0	0.0	0.0	1.7074043287492504	0.0
6	0.0	0.0	0.0	2.6521743705215752	0.0
7	0.0	0.0	0.0	3.3528340996853925	0.0
8	0.0	0.0	0.0	4.195119898147077	0.0
9	0.0	0.0	0.0	5.154094634834121	0.0
10	0.0	0.0	0.0	6.596870758320269	0.0
11	0.0	0.0	0.0	7.727354033607256	0.0
12	0.0	0.0	0.0	8.275339597428424	0.0
13	0.0	0.0	0.0	8.527341407211777	0.0
14	0.0	0.0	0.0	8.779343216995128	0.0
15	0.0	0.0	0.0	8.984522143541072	0.0
16	0.0	0.0	0.0	9.37215248476941	0.0
17	0.0	0.0	0.0	9.89677921695304	0.0
18	0.0	0.0	0.0	10.482328307326465	1.0521996233125349E-4
19	0.0	0.0	0.0	10.790622796957038	1.0521996233125349E-4
20	0.0	0.0	0.0	11.12069781879018	1.0521996233125349E-4
21	0.0	0.0	0.0	11.49496522480245	1.0521996233125349E-4
22	0.0	0.0	0.0	11.94825282252549	1.0521996233125349E-4
23	0.0	0.0	0.0	12.391018424015405	1.0521996233125349E-4
24	0.0	0.0	0.0	12.723513504982165	1.0521996233125349E-4
25	0.0	0.0	0.0	13.00266206504698	1.0521996233125349E-4
26	0.0	0.0	0.0	13.266027630762109	1.0521996233125349E-4
27	0.0	0.0	0.0	13.556539946758699	1.0521996233125349E-4
28	0.0	0.0	0.0	13.83705636633382	1.0521996233125349E-4
29	0.0	0.0	0.0	14.16607918854365	1.0521996233125349E-4
30	0.0	0.0	0.0	14.588432117341302	1.0521996233125349E-4
31	0.0	0.0	0.0	14.907879922978987	1.0521996233125349E-4
32	0.0	0.0	0.0	15.230063447637285	1.0521996233125349E-4
33	0.0	0.0	0.0	15.543829375309084	1.0521996233125349E-4
34	0.0	0.0	0.0	15.849282925956713	1.0521996233125349E-4
35	0.0	0.0	0.0	16.188091204663348	1.0521996233125349E-4
36	0.0	0.0	0.0	16.51385220804091	1.0521996233125349E-4
37	0.0	0.0	0.0	16.83961321141847	1.0521996233125349E-4
38	0.0	0.0	0.0	17.167373394080325	1.0521996233125349E-4
39	0.0	0.0	0.0	17.48787339934132	1.0521996233125349E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	40	6.8121153E-9	45.000004	1
CGACCTA	20	7.032669E-4	45.000004	33
TAACGCC	35	1.2116288E-7	45.000004	12
TCGATAG	40	6.8121153E-9	45.000004	1
GGACGTA	20	7.032669E-4	45.000004	8
CGTACTA	20	7.032669E-4	45.000004	15
TACGTCC	20	7.032669E-4	45.000004	13
CGGGTTA	85	0.0	45.0	6
CGATTAG	55	1.8189894E-12	45.0	1
ATACTCG	25	3.890294E-5	45.0	44
AATTGCG	75	0.0	45.0	1
TGTACCG	25	3.890294E-5	45.0	1
CATACCG	65	0.0	44.999996	1
AATGCGG	165	0.0	42.272728	2
ATCAGCG	80	0.0	42.187504	1
CGATAGG	155	0.0	42.09677	2
CACCACG	70	0.0	41.785717	1
ACGATAG	65	0.0	41.53846	1
TAGCCCG	55	6.002665E-11	40.909092	1
TTTGCGC	55	6.002665E-11	40.909092	13
>>END_MODULE