##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	951877
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01541165507729	33.0	31.0	34.0	30.0	34.0
2	32.21693033868872	34.0	31.0	34.0	30.0	34.0
3	32.34632941020741	34.0	31.0	34.0	30.0	34.0
4	35.914941741422474	37.0	35.0	37.0	35.0	37.0
5	35.74999080763586	37.0	35.0	37.0	35.0	37.0
6	35.75315193034394	37.0	35.0	37.0	33.0	37.0
7	35.83430842430272	37.0	35.0	37.0	35.0	37.0
8	35.98274987209482	37.0	35.0	37.0	35.0	37.0
9	37.616041778507096	39.0	37.0	39.0	35.0	39.0
10	37.17309694424805	39.0	37.0	39.0	34.0	39.0
11	37.13742006582783	39.0	37.0	39.0	34.0	39.0
12	37.05696744432316	39.0	37.0	39.0	33.0	39.0
13	37.0201202466285	39.0	37.0	39.0	33.0	39.0
14	38.23585295158934	40.0	38.0	41.0	33.0	41.0
15	38.24258176213944	40.0	38.0	41.0	33.0	41.0
16	38.202300297202264	40.0	37.0	41.0	33.0	41.0
17	38.201132079039624	40.0	37.0	41.0	33.0	41.0
18	38.14832378553111	40.0	37.0	41.0	33.0	41.0
19	38.14865681175194	40.0	37.0	41.0	33.0	41.0
20	38.07947245284843	40.0	37.0	41.0	33.0	41.0
21	38.053967056668036	40.0	37.0	41.0	33.0	41.0
22	38.019154785754885	40.0	37.0	41.0	33.0	41.0
23	37.94214798760764	40.0	37.0	41.0	33.0	41.0
24	37.83356673183615	40.0	37.0	41.0	33.0	41.0
25	37.832285053636134	40.0	37.0	41.0	33.0	41.0
26	37.757087312751544	40.0	37.0	41.0	33.0	41.0
27	37.68663913509834	40.0	37.0	41.0	33.0	41.0
28	37.6434602369844	40.0	36.0	41.0	33.0	41.0
29	37.500171765889924	40.0	36.0	41.0	32.0	41.0
30	37.51967533620415	40.0	36.0	41.0	32.0	41.0
31	37.46388871671445	40.0	36.0	41.0	32.0	41.0
32	37.40742764033588	40.0	36.0	41.0	32.0	41.0
33	37.33437723571428	39.0	36.0	41.0	32.0	41.0
34	37.26053050971922	39.0	36.0	41.0	32.0	41.0
35	37.1870924499699	39.0	36.0	41.0	31.0	41.0
36	37.14349858227481	39.0	35.0	41.0	31.0	41.0
37	37.0405535589157	39.0	35.0	41.0	31.0	41.0
38	37.04950744686551	39.0	35.0	41.0	31.0	41.0
39	37.00630858818944	39.0	35.0	41.0	31.0	41.0
40	36.8999208931406	39.0	35.0	41.0	31.0	41.0
41	36.805169155258504	39.0	35.0	41.0	31.0	41.0
42	36.71188084174741	39.0	35.0	41.0	31.0	41.0
43	36.63421639560573	39.0	35.0	40.0	31.0	41.0
44	36.56261785924022	39.0	35.0	40.0	31.0	41.0
45	36.41668724005307	38.0	35.0	40.0	30.0	41.0
46	36.28017485452427	38.0	35.0	40.0	30.0	41.0
47	36.15927372969407	38.0	35.0	40.0	30.0	41.0
48	36.09742330154001	38.0	35.0	40.0	30.0	41.0
49	35.93105306673026	38.0	35.0	40.0	29.0	41.0
50	35.77282253904654	38.0	34.0	40.0	29.0	41.0
51	34.570068401694755	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	10.0
12	4.0
13	17.0
14	32.0
15	31.0
16	67.0
17	138.0
18	276.0
19	450.0
20	749.0
21	1130.0
22	1681.0
23	2461.0
24	3152.0
25	4101.0
26	5459.0
27	7044.0
28	9113.0
29	11845.0
30	15471.0
31	20149.0
32	26672.0
33	36445.0
34	56439.0
35	69372.0
36	84927.0
37	123816.0
38	198886.0
39	271852.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.14010213504476	31.424543297085656	21.782331120512417	26.65302344735717
2	23.997113072382252	28.84595383647257	20.37185476694993	26.78507832419525
3	20.730829718545568	30.38722439979115	21.181833367126217	27.700112514537068
4	21.13581901863371	30.96177342240647	19.73784428030092	28.164563278658903
5	18.97965808607625	33.780940184498625	17.35623405124822	29.883167678176907
6	21.043265043697872	36.93029666648107	18.665541871481295	23.360896418339763
7	87.17355288550937	4.203064051342769	4.326714480967604	4.296668582180261
8	88.58329384994069	3.528922329250523	3.812887589467967	4.074896231340814
9	81.03946203133387	7.48205913158948	7.0504907671894586	4.4279880698871805
10	36.07724527433692	36.34408647335738	12.198214685300728	15.380453567004981
11	25.875086802181375	27.484538443517387	27.521412955665493	19.11896179863575
12	28.0533094086736	22.811140515003515	29.001541165507728	20.134008910815158
13	26.811972555277624	23.1934378076159	27.415621976368797	22.57896766073768
14	21.51401914322964	25.733366811048064	28.871062122522133	23.881551923200163
15	20.670948032151212	28.248397639611	28.833452221242872	22.24720210699492
16	23.02723986397402	25.570740757471818	28.79794343176692	22.60407594678724
17	22.57875754955735	26.096964208610984	28.416801750646353	22.90747649118531
18	23.28431089310909	26.4304106517964	27.052549856756702	23.23272859833781
19	23.74256337741116	29.739766797600954	25.535442079176196	20.982227745811695
20	26.37546657813982	27.235766806005397	25.204411914564588	21.184354701290186
21	25.442677993060027	27.735831415193353	24.904793371412485	21.91669722033414
22	23.268447498994092	26.05735825111858	25.065528424365752	25.608665825521577
23	23.54831559119508	27.551353798862664	24.62355955653934	24.276771053402907
24	22.003157971040377	27.325589335596934	26.865025628311223	23.806227065051473
25	23.915064656462967	27.286193489284855	24.06602953953084	24.732712314721333
26	24.998397902249973	27.35626556792527	24.612633775162127	23.03270275466263
27	22.639584736263192	26.91702814544316	24.98179911900382	25.461587999289826
28	23.64160495526208	27.869146959113415	25.601101823029655	22.88814626259485
29	23.417836548209486	27.546311130534722	24.220041034713518	24.815811286542274
30	24.49812318188169	26.508361899699224	24.470073339307493	24.523441579111587
31	24.704767527737303	27.407742807106377	22.61447645021363	25.273013214942686
32	24.708444473393097	27.91179952872062	23.494106906669664	23.885649091216617
33	24.406829874027842	27.240599363153013	24.773789050476058	23.578781712343087
34	22.976603069514233	27.487269888861693	24.263954271402714	25.272172770221363
35	23.327908963027784	27.060849248379782	25.481758672601607	24.129483115990826
36	23.515328135883102	30.311374263691633	24.207014141532994	21.96628345889227
37	24.110362998580698	29.7502723566175	23.69917541867279	22.440189226129007
38	24.7980568918043	26.202124854366687	25.88737830623074	23.112439947598272
39	23.67070535373793	25.270701991959044	26.053576249872616	25.005016404430403
40	24.677242963113933	25.974364334887806	26.61782982465171	22.730562877346546
41	22.48357718486737	26.54723246806047	27.494413668992944	23.474776678079206
42	23.14731840353323	28.30975010426767	24.749836375918317	23.793095116280778
43	22.21883709765022	26.62119160353701	26.874060409065457	24.28591088974731
44	24.078426099170375	25.37071491379664	24.960052611839558	25.59080637519343
45	23.964230672660438	24.977701950987367	26.991407503280364	24.066659873071835
46	22.28407661914302	26.059564418512053	27.205825962808223	24.450532999536705
47	22.72394437516612	27.320861834039484	26.289110883023753	23.666082907770647
48	22.666478967345572	25.399395089911824	27.436002760860912	24.49812318188169
49	22.782145172117826	26.09360242972569	26.151172893136405	24.97307950502008
50	22.210957928387806	24.46566100452054	27.014624788706943	26.30875627838471
51	22.239007770962004	24.010980410284102	28.089553587280708	25.660458231473182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	680.0
1	593.5
2	507.0
3	397.5
4	288.0
5	277.5
6	267.0
7	324.0
8	381.0
9	431.5
10	482.0
11	701.0
12	920.0
13	942.0
14	964.0
15	1031.5
16	1099.0
17	1152.5
18	1206.0
19	1325.5
20	1445.0
21	1925.0
22	2405.0
23	3045.0
24	3685.0
25	4498.0
26	5760.0
27	6209.0
28	8501.0
29	10793.0
30	12989.0
31	15185.0
32	17859.0
33	20533.0
34	22704.0
35	24875.0
36	28110.5
37	31346.0
38	33039.5
39	34733.0
40	38254.0
41	41775.0
42	44549.5
43	47324.0
44	53364.5
45	59405.0
46	66265.0
47	73125.0
48	80193.5
49	87262.0
50	84002.5
51	80743.0
52	74919.0
53	69095.0
54	62913.0
55	56731.0
56	53044.0
57	49357.0
58	49435.0
59	49513.0
60	47372.0
61	45231.0
62	42033.5
63	38836.0
64	33575.5
65	28315.0
66	23482.0
67	18649.0
68	16308.0
69	13967.0
70	12284.5
71	10602.0
72	8699.5
73	6797.0
74	6112.5
75	4170.5
76	2913.0
77	2297.0
78	1681.0
79	1325.0
80	969.0
81	698.5
82	428.0
83	311.5
84	195.0
85	151.0
86	107.0
87	71.5
88	36.0
89	27.5
90	19.0
91	21.5
92	24.0
93	28.0
94	32.0
95	17.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	951877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.08872051134574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05678347614747	19.32046726078459
2	9.750835298012019	5.087736336840004
3	3.426042288437813	2.681431791693164
4	1.7372242838548169	1.812878352280443
5	1.1288687591102695	1.4725370775208753
6	0.8035955151813441	1.2578867279842187
7	0.6169926511267187	1.1267584182959451
8	0.4829254431102693	1.007912553049729
9	0.44469910780251576	1.0441467661594177
>10	6.196221232396396	39.0938567621131
>50	1.2707191452196274	21.852674337290658
>100	0.08144596533212743	3.281844217030355
>500	0.0028364266533576717	0.49314876058774704
>1k	8.104076152450491E-4	0.4667206383697542
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	3325	0.3493098373004075	Illumina Single End Adapter 1 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1090	0.11451059328043434	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	964	0.10127358891957679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4110825243177427	0.0
2	0.0	0.0	0.0	0.5752844117464756	0.0
3	0.0	0.0	0.0	0.7577659718640118	0.0
4	0.0	0.0	0.0	1.5567137350729139	0.0
5	0.0	0.0	0.0	1.694126447009435	0.0
6	0.0	0.0	0.0	2.6408874255812465	0.0
7	0.0	0.0	0.0	3.3422385455263655	0.0
8	0.0	0.0	0.0	4.178270932063701	0.0
9	0.0	0.0	0.0	5.123876299143692	0.0
10	0.0	0.0	0.0	6.548009879427699	0.0
11	0.0	0.0	0.0	7.677987807248205	0.0
12	0.0	0.0	0.0	8.21398142827277	0.0
13	0.0	0.0	0.0	8.469686734735685	0.0
14	0.0	0.0	0.0	8.72822854213307	0.0
15	0.0	0.0	0.0	8.943382390792088	0.0
16	0.0	0.0	0.0	9.339336910125994	0.0
17	0.0	0.0	0.0	9.867451361888143	0.0
18	0.0	0.0	0.0	10.446832941651074	0.0
19	0.0	0.0	0.0	10.760633989475531	0.0
20	0.0	0.0	0.0	11.100803990431537	0.0
21	0.0	0.0	0.0	11.468393500420747	0.0
22	0.0	0.0	0.0	11.915089869804607	0.0
23	0.0	0.0	0.0	12.348129012466947	0.0
24	0.0	0.0	0.0	12.678844010308055	0.0
25	0.0	0.0	0.0	12.966486216181293	0.0
26	0.0	0.0	0.0	13.216623576365434	0.0
27	0.0	0.0	0.0	13.516452230697874	0.0
28	0.0	0.0	0.0	13.791907988111909	0.0
29	1.050555901655361E-4	0.0	0.0	14.11316798283812	0.0
30	1.050555901655361E-4	0.0	0.0	14.51910278323775	0.0
31	1.050555901655361E-4	0.0	0.0	14.827125773603102	0.0
32	1.050555901655361E-4	0.0	0.0	15.152167769575271	0.0
33	1.050555901655361E-4	0.0	0.0	15.451681257137214	0.0
34	1.050555901655361E-4	0.0	0.0	15.753295856502469	0.0
35	1.050555901655361E-4	0.0	0.0	16.088528244720695	0.0
36	1.050555901655361E-4	0.0	0.0	16.408422516774753	0.0
37	1.050555901655361E-4	0.0	0.0	16.730733067402618	0.0
38	1.050555901655361E-4	0.0	0.0	17.056510452505943	0.0
39	1.050555901655361E-4	0.0	0.0	17.38606983885523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAG	30	2.1651067E-6	45.000004	1
AACGTCG	20	7.032676E-4	45.0	1
ATTGTCG	20	7.032676E-4	45.0	44
TAACGTC	25	3.890299E-5	45.0	32
ATATGCG	50	2.1827873E-11	45.0	1
TAACGCC	25	3.890299E-5	45.0	12
CATTACG	35	1.2116288E-7	45.0	1
CGTTACG	20	7.032676E-4	45.0	1
TGCGACG	35	1.2116288E-7	45.0	1
TCATACG	25	3.890299E-5	45.0	1
CGCGTAG	20	7.032676E-4	45.0	1
CGGTACG	20	7.032676E-4	45.0	6
CGTTTAG	45	3.8380676E-10	45.0	1
TGTCGAC	20	7.032676E-4	45.0	16
TCGAAAC	20	7.032676E-4	45.0	45
AGTCGTG	20	7.032676E-4	45.0	24
AATTGCG	85	0.0	42.352943	1
TCATGCG	70	0.0	41.785713	1
TAACGCG	65	0.0	41.538464	1
CGATAAG	60	3.6379788E-12	41.250004	1
>>END_MODULE