FastQCFastQC Report
Sat 18 Jun 2016
SRR3553522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935337
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT56200.600852954603528No Hit
AATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCT50520.5401261791204668No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC45910.4908391307090386No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGT41870.44764614251334006No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT11890.12711995783337984No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC10400.1111898706027881No Hit
AACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG10060.10755481714077386No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT9940.10627185709535707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG253.8902468E-545.013
CTTCGAT207.032613E-445.016
TCGTTCA253.8902468E-545.016
CAACGCG502.1827873E-1145.01
CGACGGT253.8902468E-545.028
AACCCGC207.032613E-445.043
CGTGAAA207.032613E-445.042
TTCCGCG453.8380676E-1045.01
CGATTAC253.8902468E-545.019
TCGATGA207.032613E-445.018
TCGATAG207.032613E-445.01
CATTACG253.8902468E-545.01
TATTGCG253.8902468E-545.01
TCGCTAG207.032613E-445.01
CGAATCG253.8902468E-545.01
TCGTACG253.8902468E-545.01
CTCGATG253.8902468E-545.01
TATAGCG453.8380676E-1045.01
CGTAACG850.045.01
ACGTACG700.045.01