##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935337
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875612747063357	33.0	31.0	34.0	30.0	34.0
2	32.08796080984715	34.0	31.0	34.0	30.0	34.0
3	32.21207971030762	34.0	31.0	34.0	30.0	34.0
4	35.83485310642047	37.0	35.0	37.0	35.0	37.0
5	35.627418780610626	37.0	35.0	37.0	33.0	37.0
6	35.62804956929962	37.0	35.0	37.0	33.0	37.0
7	35.74607120214425	37.0	35.0	37.0	35.0	37.0
8	35.96484475648884	37.0	35.0	37.0	35.0	37.0
9	37.5664493118523	39.0	37.0	39.0	35.0	39.0
10	37.04253974770591	39.0	37.0	39.0	33.0	39.0
11	37.029125331297706	39.0	37.0	39.0	33.0	39.0
12	37.053603139830884	39.0	37.0	39.0	33.0	39.0
13	37.05757069377134	39.0	37.0	39.0	33.0	39.0
14	38.280135395050124	40.0	38.0	41.0	33.0	41.0
15	38.28469311061147	40.0	38.0	41.0	33.0	41.0
16	38.18608159412062	40.0	37.0	41.0	33.0	41.0
17	38.182990729544535	40.0	37.0	41.0	33.0	41.0
18	38.14449444424844	40.0	37.0	41.0	33.0	41.0
19	38.14750191642157	40.0	37.0	41.0	33.0	41.0
20	38.120862320211856	40.0	37.0	41.0	33.0	41.0
21	38.086511064995825	40.0	37.0	41.0	33.0	41.0
22	38.08560550903044	40.0	37.0	41.0	33.0	41.0
23	37.97705319045435	40.0	37.0	41.0	33.0	41.0
24	37.88878233192956	40.0	37.0	41.0	33.0	41.0
25	37.90793906367438	40.0	37.0	41.0	33.0	41.0
26	37.85548951875099	40.0	37.0	41.0	33.0	41.0
27	37.82326690807698	40.0	37.0	41.0	33.0	41.0
28	37.7876562137497	40.0	37.0	41.0	33.0	41.0
29	37.68602225721852	40.0	37.0	41.0	33.0	41.0
30	37.68740037013397	40.0	37.0	41.0	33.0	41.0
31	37.613064595969156	40.0	37.0	41.0	33.0	41.0
32	37.60014946484529	40.0	37.0	41.0	33.0	41.0
33	37.51450332874675	40.0	37.0	41.0	32.0	41.0
34	37.527576691609546	40.0	37.0	41.0	32.0	41.0
35	37.48161144058238	40.0	37.0	41.0	32.0	41.0
36	37.412405368332486	40.0	36.0	41.0	32.0	41.0
37	37.358037798141204	39.0	36.0	41.0	32.0	41.0
38	37.32829878428844	39.0	36.0	41.0	32.0	41.0
39	37.250348270195666	39.0	36.0	41.0	31.0	41.0
40	37.15704500089273	39.0	36.0	41.0	31.0	41.0
41	37.13055294508824	39.0	36.0	41.0	31.0	41.0
42	37.081223131341964	39.0	36.0	41.0	31.0	41.0
43	37.01853877265627	39.0	36.0	41.0	31.0	41.0
44	36.92679109240841	39.0	35.0	41.0	31.0	41.0
45	36.76581916464333	39.0	35.0	40.0	31.0	41.0
46	36.64553952211877	39.0	35.0	40.0	30.0	41.0
47	36.59008357415563	39.0	35.0	40.0	30.0	41.0
48	36.55166640472899	39.0	35.0	40.0	30.0	41.0
49	36.38798101646786	39.0	35.0	40.0	30.0	41.0
50	36.249126250752404	39.0	35.0	40.0	30.0	41.0
51	35.034976698238175	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	4.0
12	5.0
13	6.0
14	4.0
15	29.0
16	50.0
17	78.0
18	163.0
19	357.0
20	649.0
21	1076.0
22	1612.0
23	2078.0
24	2812.0
25	3609.0
26	4666.0
27	6236.0
28	8125.0
29	10937.0
30	14501.0
31	19446.0
32	25888.0
33	34614.0
34	49431.0
35	63829.0
36	82006.0
37	120711.0
38	202160.0
39	280173.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.94664703737797	32.876278817153604	20.98270462945441	27.194369516014017
2	22.490289596156252	30.553052001578042	20.623796556749067	26.332861845516643
3	19.194258326143412	30.53380760089679	23.314591425336538	26.95734264762326
4	21.659359140074645	31.230668732232342	19.306624243454497	27.803347884238516
5	17.87665835950037	34.62837458584446	18.31404082165038	29.18092623300479
6	20.464495684443147	36.127192658902615	20.640688864013722	22.767622792640513
7	86.066412426751	4.53954029403306	4.987079523209282	4.406967756006659
8	87.88436681110659	2.8488127808479726	3.6733284367024934	5.5934919713429485
9	80.17324237146612	8.018500283854909	7.446727756947497	4.361529587731481
10	28.9472136780647	45.40245922058039	12.505118476014527	13.145208625340384
11	19.58908928012043	25.716613370368112	36.02156228182997	18.672735067681486
12	23.695951298836675	23.29352949792428	34.202859504114556	18.80765969912449
13	24.528913108323525	25.241276673541197	30.286089398794232	19.943720819341053
14	18.99272668567586	26.791733888427377	32.34352965829429	21.872009767602478
15	18.038632065234243	30.11930459289005	30.72732074108049	21.11474260079522
16	20.56093151452364	28.340587403256794	29.835984249527176	21.26249683269239
17	19.579894733128274	28.391798891736347	31.02293611821194	21.005370256923438
18	20.673404345171846	27.61678411096749	28.981105205931122	22.728706337929538
19	20.61481583643115	30.691184033134583	29.557261179660376	19.136738950773893
20	22.976638366706332	29.348780172280154	28.05021077964413	19.62437068136939
21	21.770335184003198	28.88894590933535	27.642977878561414	21.697741028100033
22	20.376292181320743	27.1641130416096	28.768775318414647	23.690819458655007
23	20.83078077740964	29.252130515525423	26.65745073700709	23.25963797005785
24	18.10245932749373	30.186980735285786	28.900064896395634	22.810495040824858
25	19.602560360597305	29.267205296059068	27.192872729294358	23.93736161404927
26	22.858071475842397	29.70437393153484	26.381507413905364	21.056047178717403
27	20.599420315886146	27.50174536022845	27.781323736792196	24.117510587093207
28	20.50961311270697	28.147074263073097	28.40644601892152	22.936866605298412
29	20.992861396480627	27.841515945589663	29.32365553805741	21.8419671198723
30	22.99663116074741	26.571599327301282	26.63360906282976	23.798160449121546
31	21.84635056669414	29.273940836297506	25.677910742331374	23.20179785467698
32	22.97995268015699	26.235784535413437	27.83873619882459	22.945526585604973
33	21.674861573956765	28.50822751585792	26.405669828094048	23.411241082091266
34	20.02550952223637	25.843305674853024	28.52672352317935	25.60446127973126
35	21.487549407325915	27.246222484516274	28.67340862170533	22.592819486452477
36	21.273615819752667	28.150388576523756	29.18787559991746	21.388120003806115
37	22.16495231130598	29.22411922120049	26.631256969413165	21.97967149808037
38	21.957326610622697	28.432212133166978	28.658761494520157	20.951699761690172
39	20.670945338418132	27.194155689339777	28.188342811200666	23.94655616104142
40	22.748271478622144	27.566962495870474	26.935104673502707	22.74966135200468
41	21.415062164759867	25.751787858279958	29.360647552700257	23.47250242425992
42	23.66312890434143	25.933005964695077	27.100392692687237	23.303472438276255
43	21.653585819870273	25.94241433836147	28.8111129999134	23.59288684185486
44	22.776496599621314	25.899862830188475	26.980863581789237	24.34277698840097
45	23.121185198489954	26.659375177075212	27.95217124950686	22.26726837492797
46	19.020524153326555	26.391984920942935	31.254617319746785	23.332873605983725
47	21.306331300910795	27.33934400114611	27.631645064826905	23.722679633116194
48	21.539402375828175	24.513303761104286	30.266951911450096	23.68034195161744
49	22.0177326460944	25.562337424906744	28.29931885512922	24.120611073869632
50	20.72525731367411	24.91989518216429	27.830290045192267	26.524557458969333
51	20.47935663830256	24.133654500998038	30.67311567916163	24.713873181537778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	872.0
1	727.0
2	582.0
3	419.5
4	257.0
5	252.5
6	248.0
7	275.5
8	303.0
9	366.0
10	429.0
11	514.5
12	600.0
13	642.0
14	684.0
15	848.5
16	1013.0
17	1290.0
18	1567.0
19	1795.0
20	2023.0
21	2491.5
22	2960.0
23	4039.0
24	5118.0
25	5931.0
26	8390.5
27	10037.0
28	12069.5
29	14102.0
30	17411.0
31	20720.0
32	23025.0
33	25330.0
34	27784.5
35	30239.0
36	35313.5
37	40388.0
38	43526.0
39	46664.0
40	48840.5
41	51017.0
42	54154.0
43	57291.0
44	68112.0
45	78933.0
46	86164.0
47	93395.0
48	96463.5
49	99532.0
50	92367.5
51	85203.0
52	75288.0
53	65373.0
54	58269.5
55	51166.0
56	44080.0
57	36994.0
58	33033.5
59	29073.0
60	26258.5
61	23444.0
62	21383.5
63	19323.0
64	16397.0
65	13471.0
66	10273.0
67	7075.0
68	5685.5
69	4296.0
70	3706.0
71	3116.0
72	2612.5
73	2109.0
74	1883.0
75	1384.5
76	1112.0
77	694.5
78	277.0
79	255.5
80	234.0
81	178.5
82	123.0
83	108.5
84	94.0
85	66.5
86	39.0
87	35.5
88	32.0
89	37.5
90	43.0
91	25.0
92	7.0
93	10.0
94	13.0
95	11.5
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	935337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.30267786660405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07621225676968	19.221000707013957
2	11.06601890074776	5.8213186082424055
3	4.1248090932633685	3.254805745240366
4	2.0122369047078625	2.117088763832934
5	1.2093830704321284	1.5905006659450396
6	0.7990430822048943	1.2610183675664255
7	0.6080911904205709	1.1196098686606442
8	0.4924010007694313	1.0361171923545445
9	0.396376178496271	0.9383179443284668
>10	4.71897942358496	29.46405904992295
>50	1.271818196361864	23.24994044707551
>100	0.21768757144465006	7.780059209944447
>500	0.004084194586203566	0.709221530508999
>1k	0.002042097293101783	1.2904995828543784
>5k	8.168389172407132E-4	1.1464423165089423
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	5620	0.600852954603528	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCT	5052	0.5401261791204668	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC	4591	0.4908391307090386	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGT	4187	0.44764614251334006	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT	1189	0.12711995783337984	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	1040	0.1111898706027881	No Hit
AACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	1006	0.10755481714077386	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT	994	0.10627185709535707	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0691333711806546E-4	0.0	0.0	0.6838177042071467	0.0
2	1.0691333711806546E-4	0.0	0.0	0.9424410666957471	0.0
3	1.0691333711806546E-4	0.0	0.0	1.2964311258936618	0.0
4	1.0691333711806546E-4	0.0	0.0	2.933060490497008	0.0
5	1.0691333711806546E-4	0.0	0.0	3.168269832156752	0.0
6	1.0691333711806546E-4	0.0	0.0	4.450588397550829	0.0
7	1.0691333711806546E-4	0.0	0.0	5.439002199207344	0.0
8	1.0691333711806546E-4	0.0	0.0	6.420038980602714	0.0
9	1.0691333711806546E-4	0.0	0.0	8.036889377839218	0.0
10	1.0691333711806546E-4	0.0	0.0	9.887986896701403	0.0
11	1.0691333711806546E-4	0.0	0.0	11.822156078504324	0.0
12	1.0691333711806546E-4	0.0	0.0	12.599095299341307	0.0
13	1.0691333711806546E-4	0.0	0.0	12.947739691683319	0.0
14	1.0691333711806546E-4	0.0	0.0	13.327068211778215	0.0
15	1.0691333711806546E-4	0.0	0.0	13.6086779417472	0.0
16	1.0691333711806546E-4	0.0	0.0	14.189217362298294	0.0
17	1.0691333711806546E-4	0.0	0.0	14.976527176835729	0.0
18	1.0691333711806546E-4	0.0	0.0	15.98397155249926	0.0
19	1.0691333711806546E-4	0.0	0.0	16.424347588088573	0.0
20	1.0691333711806546E-4	0.0	0.0	16.916576592180146	0.0
21	1.0691333711806546E-4	0.0	0.0	17.44173490410408	0.0
22	1.0691333711806546E-4	0.0	0.0	18.073699639808968	0.0
23	1.0691333711806546E-4	0.0	0.0	18.638202059792352	0.0
24	1.0691333711806546E-4	0.0	0.0	19.055912467912634	0.0
25	2.1382667423613092E-4	0.0	0.0	19.417065720697458	0.0
26	2.1382667423613092E-4	0.0	0.0	19.74486201230145	0.0
27	2.1382667423613092E-4	0.0	0.0	20.132422859354435	0.0
28	2.1382667423613092E-4	0.0	0.0	20.519556053058952	0.0
29	2.1382667423613092E-4	0.0	0.0	20.943788174743435	0.0
30	2.1382667423613092E-4	0.0	0.0	21.56516849007363	0.0
31	2.1382667423613092E-4	0.0	0.0	21.976357184629713	0.0
32	2.1382667423613092E-4	0.0	0.0	22.36883604519013	0.0
33	2.1382667423613092E-4	0.0	0.0	22.76901266602305	0.0
34	2.1382667423613092E-4	0.0	0.0	23.14427847930746	0.0
35	2.1382667423613092E-4	0.0	0.0	23.58240933481729	0.0
36	2.1382667423613092E-4	0.0	0.0	23.984082742369864	0.0
37	3.207400113541964E-4	0.0	0.0	24.385649236585316	0.0
38	3.207400113541964E-4	0.0	0.0	24.774172303672366	0.0
39	3.207400113541964E-4	0.0	0.0	25.186430131599625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.8902468E-5	45.0	13
CTTCGAT	20	7.032613E-4	45.0	16
TCGTTCA	25	3.8902468E-5	45.0	16
CAACGCG	50	2.1827873E-11	45.0	1
CGACGGT	25	3.8902468E-5	45.0	28
AACCCGC	20	7.032613E-4	45.0	43
CGTGAAA	20	7.032613E-4	45.0	42
TTCCGCG	45	3.8380676E-10	45.0	1
CGATTAC	25	3.8902468E-5	45.0	19
TCGATGA	20	7.032613E-4	45.0	18
TCGATAG	20	7.032613E-4	45.0	1
CATTACG	25	3.8902468E-5	45.0	1
TATTGCG	25	3.8902468E-5	45.0	1
TCGCTAG	20	7.032613E-4	45.0	1
CGAATCG	25	3.8902468E-5	45.0	1
TCGTACG	25	3.8902468E-5	45.0	1
CTCGATG	25	3.8902468E-5	45.0	1
TATAGCG	45	3.8380676E-10	45.0	1
CGTAACG	85	0.0	45.0	1
ACGTACG	70	0.0	45.0	1
>>END_MODULE