FastQCFastQC Report
Sat 18 Jun 2016
SRR3553520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805684
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT64490.8004378888000754No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT23800.2954011746540827No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC17380.21571732838184696No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT14900.18493602951032911No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC12950.16073299209119207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.11058926328436458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA253.8897637E-545.027
ATTTACG207.0320297E-445.01
TTTAGCG207.0320297E-445.01
ACCGGTC253.8897637E-545.04
TCGCACG207.0320297E-445.01
GGTAACG253.8897637E-545.01
ATAGGCG406.8102963E-945.01
CCTAGCG351.2113196E-745.01
TTTCGCG253.8897637E-545.01
TTAGACG207.0320297E-445.01
GAGTACG253.8897637E-545.01
CGCATAG453.8380676E-1045.01
TTCGTTA253.8897637E-545.012
CGCGATG207.0320297E-445.01
CGATTAG207.0320297E-445.01
CGCGACG351.2113196E-745.01
CCTCGCG406.8102963E-945.01
CGTTACG502.1827873E-1145.01
CTACGCG253.8897637E-545.01
CGCTAAG207.0320297E-445.01