##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805684
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.764006732167946	31.0	31.0	34.0	30.0	34.0
2	31.973544218328772	33.0	31.0	34.0	30.0	34.0
3	32.11525858773415	34.0	31.0	34.0	30.0	34.0
4	35.75793363154785	37.0	35.0	37.0	33.0	37.0
5	35.55490862422488	37.0	35.0	37.0	33.0	37.0
6	35.5674681388733	37.0	35.0	37.0	33.0	37.0
7	35.739426375601354	37.0	35.0	37.0	35.0	37.0
8	35.93183183481365	37.0	35.0	37.0	35.0	37.0
9	37.522627978214786	39.0	37.0	39.0	35.0	39.0
10	36.99824869303598	39.0	37.0	39.0	33.0	39.0
11	36.99714900631017	39.0	37.0	39.0	33.0	39.0
12	37.03560204745285	39.0	37.0	39.0	33.0	39.0
13	36.995757641953915	39.0	37.0	39.0	33.0	39.0
14	38.170121288247	40.0	37.0	41.0	33.0	41.0
15	38.16332457886715	40.0	37.0	41.0	33.0	41.0
16	38.07673728161413	40.0	37.0	41.0	33.0	41.0
17	38.08175041331341	40.0	37.0	41.0	33.0	41.0
18	38.05863340962462	40.0	37.0	41.0	33.0	41.0
19	38.05953823086967	40.0	37.0	41.0	33.0	41.0
20	38.02471564533986	40.0	37.0	41.0	33.0	41.0
21	37.98908877425889	40.0	37.0	41.0	33.0	41.0
22	37.977879664980314	40.0	37.0	41.0	33.0	41.0
23	37.86747533772546	40.0	37.0	41.0	33.0	41.0
24	37.7741744902468	40.0	37.0	41.0	33.0	41.0
25	37.79143311769875	40.0	37.0	41.0	33.0	41.0
26	37.755517051350154	40.0	37.0	41.0	33.0	41.0
27	37.7045144250103	40.0	37.0	41.0	33.0	41.0
28	37.640582411963	40.0	37.0	41.0	32.0	41.0
29	37.54064869105009	40.0	37.0	41.0	32.0	41.0
30	37.461077792285806	39.0	36.0	41.0	32.0	41.0
31	37.37953217390441	39.0	36.0	41.0	32.0	41.0
32	37.33310826577169	39.0	36.0	41.0	31.0	41.0
33	37.25705859865654	39.0	36.0	41.0	31.0	41.0
34	37.200243519791876	39.0	36.0	41.0	31.0	41.0
35	37.12288440629329	39.0	36.0	41.0	31.0	41.0
36	36.98153742658412	39.0	35.0	41.0	31.0	41.0
37	36.839893804518894	39.0	35.0	40.0	30.0	41.0
38	36.916340649683995	39.0	35.0	40.0	31.0	41.0
39	36.83192293752886	39.0	35.0	40.0	31.0	41.0
40	36.762112689342224	39.0	35.0	40.0	30.0	41.0
41	36.669514350539416	39.0	35.0	40.0	30.0	41.0
42	36.60455836283208	39.0	35.0	40.0	30.0	41.0
43	36.53917044399541	39.0	35.0	40.0	30.0	41.0
44	36.40945209288009	39.0	35.0	40.0	30.0	41.0
45	36.24360791575854	39.0	35.0	40.0	29.0	41.0
46	36.072795289468324	38.0	35.0	40.0	28.0	41.0
47	35.99966363983894	38.0	35.0	40.0	28.0	41.0
48	35.9117296607603	38.0	35.0	40.0	28.0	41.0
49	35.73954428783493	38.0	34.0	40.0	28.0	41.0
50	35.600014397704314	38.0	34.0	40.0	28.0	41.0
51	34.3189290093883	37.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	4.0
13	7.0
14	9.0
15	15.0
16	40.0
17	83.0
18	164.0
19	329.0
20	577.0
21	893.0
22	1401.0
23	1991.0
24	2721.0
25	3608.0
26	4738.0
27	6267.0
28	8459.0
29	11155.0
30	14443.0
31	19077.0
32	24711.0
33	32756.0
34	46106.0
35	58799.0
36	75700.0
37	109590.0
38	172510.0
39	209466.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.809007005227855	32.08031933115216	21.4234116601546	26.687262003465378
2	21.905238282006344	31.31115921378605	21.159784729496923	25.623817774710684
3	20.43083888968876	29.997741049840883	22.582054502757906	26.989365557712453
4	19.899489129733244	30.8972252148485	20.701664672501874	28.501620982916382
5	18.008797493806505	33.90572482511754	19.26077717814925	28.824700502926703
6	21.23959269390977	34.78175066154969	20.03353672159308	23.94511992294746
7	85.44267976030304	3.9705393181445827	5.655691313219575	4.931089608332796
8	87.29303300053122	3.1092835404451376	4.294860019561019	5.302823439462618
9	78.56020474528475	8.166104825216834	8.708749335967948	4.564941093530466
10	28.908852602260936	44.7982087269947	12.581731795592315	13.711206875152046
11	19.668877624478085	25.959184990641493	35.92698874496701	18.444948639913413
12	24.266958261551675	22.894087508253858	34.15569876030801	18.683255469886458
13	24.409445886973057	24.243748169257426	30.77571355519037	20.571092388579146
14	19.11469012665015	27.307232115817122	31.870435555379036	21.7076422021537
15	17.616832405757098	29.811067366362	30.59040517125821	21.981695056622698
16	19.99443950730063	28.888993699763184	29.653189091504856	21.46337770143133
17	18.781432919109726	28.89333783468457	31.023453363849846	21.301775882355862
18	19.918851559668553	27.689764225180095	28.95440395986516	23.43698025528619
19	20.37362042686711	31.159486845959457	29.428038784436577	19.038853942736857
20	22.59037041817884	29.5584869502187	27.96766474200803	19.88347788959443
21	21.510294358582275	30.048505369350764	26.874059805084872	21.567140466982092
22	20.019511371704045	27.840195411600575	28.352182741620783	23.788110475074596
23	20.933765595444367	29.213190283039008	25.942553159799626	23.910490961717002
24	17.672313214610195	29.77854841352193	28.238490524821145	24.31064784704673
25	20.46037900715417	29.806474995159395	26.577293330884068	23.155852666802367
26	22.90724403115862	29.729025275418152	25.484060748382742	21.879669945040487
27	19.926422766245825	27.357996435327003	28.07080691685574	24.644773881571435
28	22.436960396383693	28.159675505533187	28.143912501675594	21.259451596407526
29	24.027782604594357	27.676607702275334	25.784674884942483	22.510934808187827
30	24.030513203687796	26.751803436583078	27.125895512384506	22.091787847344616
31	23.878468481439374	30.486270051285615	23.396269505165797	22.238991962109214
32	25.526385034331078	27.644833458278928	25.370492649723715	21.45828885766628
33	24.589541308999557	28.448250182453666	25.602221218244374	21.359987290302403
34	24.06005332115321	28.598557250733535	25.518689709613195	21.822699718500058
35	25.79174961895731	27.6682917868544	26.100803789078597	20.4391548051097
36	23.760928602280796	29.552901633891203	28.26703769716167	18.419132066666336
37	22.271635033089897	31.80266705060545	26.006225765933046	19.919472150371607
38	24.21023627129247	29.383604490097852	27.474419251220084	18.931739987389598
39	24.850065286141962	27.6490534750597	25.643428440927213	21.857452797871126
40	25.31674949483917	29.843958673623906	24.75573549927763	20.0835563322593
41	21.44389115335541	28.898674914730837	28.58465601898511	21.072777912928643
42	24.431663034142417	29.61893248469623	25.609668306681034	20.339736174480315
43	21.76858420919368	27.150719140506702	28.28528306383148	22.795413586468143
44	24.151900745205314	25.289443503904756	26.378207833344092	24.180447917545838
45	24.99863470045328	25.985622154591624	27.46138684645593	21.554356298499165
46	22.246314932405262	26.325829978006265	29.31335362251205	22.114501467076423
47	23.062764061344147	27.283277314679204	26.671374881467173	22.982583742509473
48	22.462776969630774	25.831343305812204	30.001216357777988	21.704663366779034
49	22.815396607106507	25.970976213999535	27.67238768549456	23.541239493399395
50	21.476534224336092	24.57179241489219	26.84253379736969	27.109139563402028
51	21.87594640082216	23.69179479796049	30.168031138759115	24.264227662458236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1180.0
1	941.0
2	702.0
3	557.0
4	412.0
5	382.0
6	352.0
7	400.5
8	449.0
9	531.0
10	613.0
11	766.0
12	919.0
13	990.0
14	1061.0
15	1204.0
16	1347.0
17	1466.0
18	1585.0
19	1857.0
20	2129.0
21	2351.5
22	2574.0
23	3245.5
24	3917.0
25	4810.0
26	6997.5
27	8292.0
28	9947.5
29	11603.0
30	13271.0
31	14939.0
32	18070.0
33	21201.0
34	23781.0
35	26361.0
36	27973.5
37	29586.0
38	31004.5
39	32423.0
40	35348.0
41	38273.0
42	41955.5
43	45638.0
44	55157.0
45	64676.0
46	72884.0
47	81092.0
48	88551.5
49	96011.0
50	90240.0
51	84469.0
52	74025.5
53	63582.0
54	53063.0
55	42544.0
56	37237.5
57	31931.0
58	29698.0
59	27465.0
60	24207.5
61	20950.0
62	18887.5
63	16825.0
64	13380.5
65	9936.0
66	7809.5
67	5683.0
68	4435.0
69	3187.0
70	2437.0
71	1687.0
72	1554.0
73	1421.0
74	1421.5
75	1060.5
76	699.0
77	541.5
78	384.0
79	271.5
80	159.0
81	142.5
82	126.0
83	96.5
84	67.0
85	64.0
86	61.0
87	38.0
88	15.0
89	12.5
90	10.0
91	7.5
92	5.0
93	4.0
94	3.0
95	5.0
96	7.0
97	5.0
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	805684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.9919161097859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07484177415266	23.3780420777457
2	11.348978027329244	7.261511059642413
3	4.094475486496977	3.9297034883275828
4	2.1354041079877266	2.7326267633294212
5	1.2692158007951921	2.0302322712127263
6	0.8532622468303225	1.6378496532145868
7	0.6654678103980607	1.4902713254812368
8	0.5170379169223552	1.3232826931006751
9	0.4444770129085684	1.279770417872923
>10	4.857214977325311	34.8182621381755
>50	0.6706503417065309	14.190726723775313
>100	0.06351888720324121	3.5059178278111682
>500	0.0035071778210378573	0.7572343676169914
>1k	0.0015587456982390478	0.8605842673131531
>5k	3.8968642455976195E-4	0.803984925380635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	6449	0.8004378888000754	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	2380	0.2954011746540827	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	1738	0.21571732838184696	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	1490	0.18493602951032911	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1295	0.16073299209119207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.11058926328436458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2411814061095915E-4	0.0	0.0	0.9245560294110347	0.0
2	1.2411814061095915E-4	0.0	0.0	1.3069640206333997	0.0
3	1.2411814061095915E-4	0.0	0.0	1.7356680783036524	0.0
4	1.2411814061095915E-4	0.0	0.0	3.689535847801371	0.0
5	1.2411814061095915E-4	0.0	0.0	3.993749410438832	0.0
6	1.2411814061095915E-4	0.0	0.0	5.636577119565487	0.0
7	1.2411814061095915E-4	0.0	0.0	6.917600448811196	0.0
8	1.2411814061095915E-4	0.0	0.0	8.107148708426628	0.0
9	1.2411814061095915E-4	0.0	0.0	9.857710963603596	0.0
10	1.2411814061095915E-4	0.0	0.0	12.264858182612539	0.0
11	1.2411814061095915E-4	0.0	0.0	14.56067142949345	0.0
12	1.2411814061095915E-4	0.0	0.0	15.652538712448056	0.0
13	1.2411814061095915E-4	0.0	0.0	16.131510617065747	0.0
14	1.2411814061095915E-4	0.0	0.0	16.625749052978588	0.0
15	1.2411814061095915E-4	0.0	0.0	17.029381246245425	0.0
16	1.2411814061095915E-4	0.0	0.0	17.786377785831665	0.0
17	1.2411814061095915E-4	0.0	0.0	18.891277473550424	0.0
18	1.2411814061095915E-4	0.0	0.0	20.12277766469236	0.0
19	1.2411814061095915E-4	0.0	0.0	20.74783662080915	0.0
20	1.2411814061095915E-4	0.0	0.0	21.39896038645424	0.0
21	1.2411814061095915E-4	0.0	0.0	22.109536741451983	0.0
22	1.2411814061095915E-4	0.0	0.0	22.997726155664008	0.0
23	1.2411814061095915E-4	0.0	0.0	23.794812854667587	0.0
24	1.2411814061095915E-4	0.0	0.0	24.365259828915555	0.0
25	1.2411814061095915E-4	0.0	0.0	24.86421475417161	0.0
26	1.2411814061095915E-4	0.0	0.0	25.32357599257277	0.0
27	1.2411814061095915E-4	0.0	0.0	25.84797513665407	0.0
28	1.2411814061095915E-4	0.0	0.0	26.360955411799168	0.0
29	1.2411814061095915E-4	0.0	0.0	26.939966537749292	0.0
30	1.2411814061095915E-4	0.0	0.0	27.689640107039484	0.0
31	1.2411814061095915E-4	0.0	0.0	28.212301597152234	0.0
32	1.2411814061095915E-4	0.0	0.0	28.723792454609995	0.0
33	1.2411814061095915E-4	0.0	0.0	29.235035075786538	0.0
34	1.2411814061095915E-4	0.0	0.0	29.722819368387604	0.0
35	1.2411814061095915E-4	0.0	0.0	30.27328332199721	0.0
36	2.482362812219183E-4	0.0	0.0	30.773479428659375	0.0
37	3.723544218328774E-4	0.0	0.0	31.2788884972272	0.0
38	4.964725624438366E-4	0.0	0.0	31.758605110688556	0.0
39	4.964725624438366E-4	0.0	0.0	32.26066298945989	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	25	3.8897637E-5	45.0	27
ATTTACG	20	7.0320297E-4	45.0	1
TTTAGCG	20	7.0320297E-4	45.0	1
ACCGGTC	25	3.8897637E-5	45.0	4
TCGCACG	20	7.0320297E-4	45.0	1
GGTAACG	25	3.8897637E-5	45.0	1
ATAGGCG	40	6.8102963E-9	45.0	1
CCTAGCG	35	1.2113196E-7	45.0	1
TTTCGCG	25	3.8897637E-5	45.0	1
TTAGACG	20	7.0320297E-4	45.0	1
GAGTACG	25	3.8897637E-5	45.0	1
CGCATAG	45	3.8380676E-10	45.0	1
TTCGTTA	25	3.8897637E-5	45.0	12
CGCGATG	20	7.0320297E-4	45.0	1
CGATTAG	20	7.0320297E-4	45.0	1
CGCGACG	35	1.2113196E-7	45.0	1
CCTCGCG	40	6.8102963E-9	45.0	1
CGTTACG	50	2.1827873E-11	45.0	1
CTACGCG	25	3.8897637E-5	45.0	1
CGCTAAG	20	7.0320297E-4	45.0	1
>>END_MODULE