##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866700
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88560516903196	33.0	31.0	34.0	30.0	34.0
2	32.09262951424945	34.0	31.0	34.0	30.0	34.0
3	32.226348217376255	34.0	31.0	34.0	30.0	34.0
4	35.848614284065995	37.0	35.0	37.0	35.0	37.0
5	35.65850698050075	37.0	35.0	37.0	33.0	37.0
6	35.64966539748471	37.0	35.0	37.0	33.0	37.0
7	35.69178608515057	37.0	35.0	37.0	35.0	37.0
8	35.848658128533515	37.0	35.0	37.0	35.0	37.0
9	37.44755624783662	39.0	37.0	39.0	35.0	39.0
10	37.03864312911042	39.0	37.0	39.0	33.0	39.0
11	37.03205838236991	39.0	37.0	39.0	33.0	39.0
12	36.91969770393447	39.0	37.0	39.0	33.0	39.0
13	36.866304372908736	39.0	37.0	39.0	33.0	39.0
14	38.04839275412484	40.0	37.0	41.0	33.0	41.0
15	38.05095765547479	40.0	37.0	41.0	33.0	41.0
16	37.98914503288335	40.0	37.0	41.0	33.0	41.0
17	37.97980154609438	40.0	37.0	41.0	33.0	41.0
18	37.96792084919811	40.0	37.0	41.0	33.0	41.0
19	37.94484365985924	40.0	37.0	41.0	33.0	41.0
20	37.86593515634014	40.0	37.0	41.0	33.0	41.0
21	37.81059651551863	40.0	37.0	41.0	33.0	41.0
22	37.80677050882658	40.0	37.0	41.0	33.0	41.0
23	37.68283719856929	39.0	36.0	41.0	33.0	41.0
24	37.56650513441791	39.0	36.0	41.0	32.0	41.0
25	37.5580408445829	39.0	36.0	41.0	32.0	41.0
26	37.51058728510442	39.0	36.0	41.0	32.0	41.0
27	37.466022845275184	39.0	36.0	41.0	32.0	41.0
28	37.371534556363216	39.0	36.0	41.0	32.0	41.0
29	37.245409022729895	39.0	36.0	41.0	32.0	41.0
30	37.226656282450676	39.0	36.0	41.0	31.0	41.0
31	37.123744086765896	39.0	35.0	41.0	31.0	41.0
32	37.10581400715357	39.0	35.0	41.0	31.0	41.0
33	37.03219683858313	39.0	35.0	41.0	31.0	41.0
34	36.989763470635744	39.0	35.0	41.0	31.0	41.0
35	36.89075574016384	39.0	35.0	41.0	31.0	41.0
36	36.78251990308065	39.0	35.0	40.0	31.0	41.0
37	36.695646705895925	39.0	35.0	40.0	30.0	41.0
38	36.63245990538825	39.0	35.0	40.0	30.0	41.0
39	36.51488404292142	39.0	35.0	40.0	30.0	41.0
40	36.34303334487135	38.0	35.0	40.0	30.0	41.0
41	36.27019614630206	38.0	35.0	40.0	30.0	41.0
42	36.21491981077651	38.0	35.0	40.0	30.0	41.0
43	36.153313718703124	38.0	35.0	40.0	30.0	41.0
44	36.03685704395985	38.0	35.0	40.0	30.0	41.0
45	35.82216107072805	38.0	35.0	40.0	29.0	41.0
46	35.67439021576093	38.0	34.0	40.0	29.0	41.0
47	35.567092419522325	38.0	34.0	40.0	28.0	41.0
48	35.48136840890735	38.0	34.0	40.0	28.0	41.0
49	35.3275574016384	37.0	34.0	40.0	28.0	41.0
50	35.189715010961116	37.0	34.0	40.0	27.0	41.0
51	33.91097265489789	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	3.0
12	11.0
13	13.0
14	27.0
15	37.0
16	96.0
17	155.0
18	268.0
19	502.0
20	776.0
21	1277.0
22	1841.0
23	2574.0
24	3246.0
25	4335.0
26	5786.0
27	7526.0
28	9824.0
29	12770.0
30	16383.0
31	21312.0
32	27575.0
33	37004.0
34	55979.0
35	69541.0
36	83227.0
37	119343.0
38	178551.0
39	206647.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.82485289027345	30.928002769124262	20.233414099457715	28.013730241144568
2	24.52359524633668	29.700588438906195	20.11053421022268	25.66528210453444
3	21.18945425175955	29.78896965501327	21.29029652705665	27.73127956617053
4	21.560401523018346	30.781816083996773	19.896850121149185	27.7609322718357
5	19.169724241375334	32.95938617745471	17.43232952578747	30.438560055382485
6	20.371754932502594	36.3519095419407	19.659743856005537	23.61659166955117
7	83.73104880581515	4.68443521403023	6.261105342102227	5.3234106380523825
8	85.5822083766009	3.9901926848967344	5.040844582900657	5.386754355601708
9	77.3535248644283	8.121956847813545	9.093342563747548	5.4311757240106155
10	35.72574131764163	33.44652128764278	13.923502942194531	16.90423445252106
11	28.579323872158763	27.937117803161414	24.334025614399447	19.149532710280372
12	28.70128071997231	23.49936540902273	27.695857851621092	20.103496019383872
13	26.659513095650166	23.51563401407638	26.42840659974616	23.396446290527287
14	21.541479173877928	26.864428291219568	27.917272412599516	23.67682012230299
15	20.941502249913466	27.27529710395754	28.54320987654321	23.239990769585788
16	23.166608976577823	25.293527172031844	27.377523941386873	24.16233991000346
17	21.04234452521057	27.178493134879428	27.935156340140765	23.844005999769237
18	24.075920156917043	24.989615784008308	26.381446867428178	24.553017191646475
19	24.269066574362526	29.169031960309216	24.61451482635283	21.947386638975424
20	27.019499250028844	26.218530056536288	24.685127495096342	22.076843198338526
21	25.11111111111111	28.228914272527977	23.67139725395177	22.98857736240914
22	24.117341640706126	25.556017076266297	24.45932848736587	25.867312795661707
23	25.17491634937118	27.35410176531672	23.280027691242644	24.19095419406946
24	22.222337602399907	26.717549325025963	25.578862351448024	25.48125072112611
25	23.9531556478597	27.035767855082497	23.156109380408445	25.85496711664936
26	24.815391715703242	26.565824391369564	23.831429560401524	24.78735433252567
27	22.704626745125186	25.29756547825084	23.931579554632513	28.066228221991462
28	22.102226837429328	26.645552094150226	26.359063112957195	24.893157955463252
29	23.91646475135572	27.277143186800508	22.835467866620515	25.97092419522326
30	24.132802584515982	24.91415714780201	24.19833852544133	26.754701742240684
31	23.8713511018807	26.815276335525557	22.28360447675089	27.029768085842854
32	23.73543325256721	25.817353178723895	23.082612207222798	27.364601361486095
33	21.803276797046266	26.22891427252798	24.133956386292834	27.83385254413292
34	20.296873197184723	26.173647167416636	24.806622822199145	28.722856813199492
35	21.70358832352602	25.955117110880348	24.519441559940002	27.82185300565363
36	21.049959616937812	30.229837313949464	24.07315103265259	24.64705203646014
37	21.87665859005423	29.520595361716857	22.419176185531324	26.18356986269759
38	22.324679820006924	28.23549094265605	25.166608976577827	24.273220260759203
39	22.582554517133957	26.52001846082843	24.08203530633437	26.815391715703242
40	23.264912887965846	28.016383985231336	24.253259490019616	24.465443636783203
41	20.806853582554517	26.265720549209647	27.117918541594555	25.809507326641285
42	23.45759778470059	26.823699088496593	23.76335525556709	25.955347871235723
43	22.5004038306219	24.16972424137533	26.419637706242067	26.910234221760703
44	24.530171916464752	23.427022037613938	24.708780431521866	27.334025614399447
45	24.402792200299988	23.517133956386292	26.607130494980964	25.472943348332755
46	21.050074997115495	25.062766816660897	27.48436598592362	26.40279220029999
47	22.538017768547363	26.56640129225799	25.24795200184608	25.647628937348564
48	22.083996769355025	25.380408445829005	27.391600323064498	25.143994461751472
49	22.66020537671628	24.898465443636784	25.736356293988692	26.704972885658247
50	20.928695050190377	24.071997230875734	26.33714087919695	28.662166839736937
51	21.7529710395754	23.355832467982	28.324218299296184	26.56697819314642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1288.0
1	1052.0
2	816.0
3	573.5
4	331.0
5	331.5
6	332.0
7	366.5
8	401.0
9	453.5
10	506.0
11	574.5
12	643.0
13	685.5
14	728.0
15	779.5
16	831.0
17	831.5
18	832.0
19	938.5
20	1045.0
21	1393.0
22	1741.0
23	2144.0
24	2547.0
25	2958.0
26	4743.0
27	6117.0
28	7147.0
29	8177.0
30	9888.0
31	11599.0
32	13417.5
33	15236.0
34	17087.5
35	18939.0
36	21000.5
37	23062.0
38	24402.5
39	25743.0
40	27587.5
41	29432.0
42	34494.0
43	39556.0
44	45237.5
45	50919.0
46	58514.5
47	66110.0
48	74405.5
49	82701.0
50	80633.5
51	78566.0
52	73477.5
53	68389.0
54	63371.0
55	58353.0
56	54453.0
57	50553.0
58	48990.0
59	47427.0
60	45180.5
61	42934.0
62	39920.5
63	36907.0
64	30749.0
65	24591.0
66	21414.5
67	18238.0
68	17079.0
69	15920.0
70	13281.5
71	10643.0
72	9129.0
73	7615.0
74	6552.5
75	4712.5
76	3935.0
77	2904.0
78	1873.0
79	1457.0
80	1041.0
81	754.5
82	468.0
83	427.5
84	387.0
85	277.0
86	167.0
87	150.0
88	133.0
89	82.5
90	32.0
91	30.5
92	29.0
93	16.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	866700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.340161998124977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68452069516734	19.672023738630248
2	10.359241663620447	5.457282071949765
3	3.7664819941899053	2.9762923766994875
4	1.896519547800559	1.9981852848670982
5	1.1022407514780168	1.451659997743299
6	0.7106238814701497	1.1230768894596062
7	0.523075826821583	0.9644531411048565
8	0.4156307680085067	0.8758225408599333
9	0.32983241096221005	0.7819055223279071
>10	4.540196122618882	30.172698514164985
>50	1.532260030905666	27.344492718520375
>100	0.13278121356762917	5.33898226581049
>500	0.005715747603904568	1.0620985739950979
>1k	4.396728926080437E-4	0.13341375610537048
>5k	4.396728926080437E-4	0.647612607761486
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	5592	0.6452059536171686	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1152	0.13291796469366562	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	998	0.11514941733010269	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	940	0.10845736702434522	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.10672666435906311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.755163262951425	0.0
2	0.0	0.0	0.0	1.0380754586362062	0.0
3	0.0	0.0	0.0	1.3440636898580824	0.0
4	0.0	0.0	0.0	2.681204569055036	0.0
5	0.0	0.0	0.0	2.8916580131533403	0.0
6	0.0	0.0	0.0	4.220145379023884	0.0
7	0.0	0.0	0.0	5.183454482519903	0.0
8	0.0	0.0	0.0	6.164185992846429	0.0
9	0.0	0.0	0.0	7.311295719395408	0.0
10	0.0	0.0	0.0	9.097034729433483	0.0
11	0.0	0.0	0.0	10.40683050651898	0.0
12	0.0	0.0	0.0	11.085727472020308	0.0
13	0.0	0.0	0.0	11.398638513903311	0.0
14	0.0	0.0	0.0	11.744202146071306	0.0
15	0.0	0.0	0.0	12.030575747086651	0.0
16	0.0	0.0	0.0	12.58705434406369	0.0
17	0.0	0.0	0.0	13.242529133494866	0.0
18	0.0	0.0	0.0	14.093227183569862	0.0
19	0.0	0.0	0.0	14.446636667820469	0.0
20	0.0	0.0	0.0	14.877466251298028	0.0
21	0.0	0.0	0.0	15.316372447213569	0.0
22	0.0	0.0	0.0	15.848505826698974	0.0
23	0.0	0.0	0.0	16.350640359986155	0.0
24	0.0	0.0	0.0	16.74350986500519	0.0
25	0.0	0.0	0.0	17.0969193492558	0.0
26	0.0	0.0	0.0	17.402330679589248	0.0
27	0.0	0.0	0.0	17.747778931579553	0.0
28	0.0	0.0	0.0	18.082381446867426	0.0
29	0.0	0.0	0.0	18.44375216337833	0.0
30	0.0	0.0	0.0	18.91784931348794	0.0
31	0.0	0.0	0.0	19.251182646821277	0.0
32	0.0	0.0	0.0	19.60643821391485	0.0
33	0.0	0.0	0.0	19.95996307834314	0.0
34	0.0	0.0	0.0	20.296296296296298	0.0
35	1.1538017768547363E-4	0.0	0.0	20.66989731164186	0.0
36	1.1538017768547363E-4	0.0	0.0	21.019499250028844	0.0
37	1.1538017768547363E-4	0.0	0.0	21.358024691358025	0.0
38	1.1538017768547363E-4	0.0	0.0	21.71651090342679	0.0
39	1.1538017768547363E-4	0.0	0.0	22.06265143648321	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTCG	30	2.1648812E-6	45.000004	9
ATGATCG	25	3.8900092E-5	45.0	15
GGTCGAT	20	7.0323265E-4	45.0	8
ACGCATC	25	3.8900092E-5	45.0	10
CTATCGT	20	7.0323265E-4	45.0	40
CATTGCG	25	3.8900092E-5	45.0	1
TAGCGGA	25	3.8900092E-5	45.0	3
CGATTAT	20	7.0323265E-4	45.0	33
TTATCGA	20	7.0323265E-4	45.0	37
TCGATAG	50	2.1827873E-11	45.0	1
CGTTAAG	20	7.0323265E-4	45.0	1
CTTATCG	20	7.0323265E-4	45.0	36
TACGAAT	75	0.0	45.0	11
AATTACG	55	1.8189894E-12	45.0	1
TTACGAA	20	7.0323265E-4	45.0	32
CTTTCGT	20	7.0323265E-4	45.0	45
TAGTGCG	20	7.0323265E-4	45.0	1
ACGAGTA	20	7.0323265E-4	45.0	33
TATCGTT	20	7.0323265E-4	45.0	41
TTCGACG	20	7.0323265E-4	45.0	27
>>END_MODULE