FastQCFastQC Report
Sat 18 Jun 2016
SRR3553515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874255
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT55600.6359700545035487No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11950.1366878084769318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10620.12147485573431092No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC9870.11289612298471269No Hit
AAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT9560.10935024678154542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.164903E-645.0000041
GATACGA253.890037E-545.031
CGTATAG207.0323586E-445.01
CCGATCT253.890037E-545.022
CGGTTTA207.0323586E-445.032
CGTGACG253.890037E-545.01
ATTCGTT207.0323586E-445.026
GCGCATC207.0323586E-445.022
TTCCCGA207.0323586E-445.028
TGCGCTA207.0323586E-445.026
CTACGTT253.890037E-545.043
GTATGCG253.890037E-545.01
TACGAAT1000.045.011
TCGCTAA253.890037E-545.015
TCGTAGA207.0323586E-445.036
CGTAATG406.8121153E-945.01
CGTAACG900.045.01
TAGTGCG253.890037E-545.01
TAGCTCG207.0323586E-445.030
TATCGGC207.0323586E-445.027