FastQCFastQC Report
Sat 18 Jun 2016
SRR3553514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044887
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT434212.1233936153929287TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC329191.6098200047239775No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT298611.4602762891054615No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT93690.4581671261052567RNA PCR Primer, Index 40 (96% over 26bp)
AACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG92860.4541082221169189RNA PCR Primer, Index 40 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT86390.4224683319909609No Hit
AATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT75450.368969043277208No Hit
AATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC48200.23570984606973394TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCTC43580.21311691061657684No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT43020.21037837298589118No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG21600.10562930861216292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG351.2125565E-745.0000041
TATCGCG207.034576E-445.0000041
ATTCACG551.8189894E-1245.01
GTATACG302.1663363E-644.9999961
AATCACG1250.041.3999981
TATACGG1500.040.5000042
CGATCGA501.0822987E-940.518
CGATAGG2450.040.4081652
TAACACG1350.040.01
CTACGCG4900.039.948981
TACGGCT41650.039.8139276
TACGCGG5550.039.729732
CTTAACG800.039.3750041
TAATGCG403.4599907E-739.3750041
ACGGCTG42300.039.255327
TACGAAT2700.039.16666411
CGTAACG1500.039.0000041
TGATACC41250.038.9454543
AATCTGT61050.038.6609341
ACGGGAT12700.038.6220475
Produced by FastQC (version 0.11.5)