##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553514_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2044887 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.895222083176233 33.0 31.0 34.0 30.0 34.0 2 32.099989388166684 34.0 31.0 34.0 30.0 34.0 3 32.200459487492466 34.0 31.0 34.0 30.0 34.0 4 35.79780398623494 37.0 35.0 37.0 35.0 37.0 5 35.55557495353044 37.0 35.0 37.0 33.0 37.0 6 35.536948496420585 37.0 35.0 37.0 33.0 37.0 7 35.318676777738816 37.0 35.0 37.0 33.0 37.0 8 35.67011673505675 37.0 35.0 37.0 35.0 37.0 9 37.34009458713366 39.0 37.0 39.0 34.0 39.0 10 36.96626806273403 39.0 37.0 39.0 33.0 39.0 11 36.92946896332169 39.0 37.0 39.0 33.0 39.0 12 36.71089356037766 39.0 35.0 39.0 32.0 39.0 13 36.75716457682014 39.0 35.0 39.0 33.0 39.0 14 37.88191425736483 40.0 37.0 41.0 33.0 41.0 15 37.94653494300663 40.0 37.0 41.0 33.0 41.0 16 37.753351652193984 40.0 36.0 41.0 33.0 41.0 17 37.73903154550838 40.0 36.0 41.0 33.0 41.0 18 37.65955380419554 40.0 36.0 41.0 32.0 41.0 19 37.60821796021003 40.0 36.0 41.0 32.0 41.0 20 37.687986671146135 40.0 36.0 41.0 32.0 41.0 21 37.712840367218334 40.0 36.0 41.0 33.0 41.0 22 37.69514647997664 40.0 36.0 41.0 33.0 41.0 23 37.58967952752401 39.0 36.0 41.0 32.0 41.0 24 37.49581223803565 39.0 36.0 41.0 32.0 41.0 25 37.42702995324436 39.0 36.0 41.0 32.0 41.0 26 37.383820230653335 39.0 36.0 41.0 32.0 41.0 27 37.31634608660527 39.0 36.0 41.0 32.0 41.0 28 37.19409874482062 39.0 36.0 41.0 31.0 41.0 29 37.08845916669234 39.0 35.0 41.0 31.0 41.0 30 37.05163806117404 39.0 35.0 41.0 31.0 41.0 31 37.00143479810865 39.0 35.0 41.0 31.0 41.0 32 36.86483165084427 39.0 35.0 41.0 31.0 41.0 33 36.88523424521746 39.0 35.0 41.0 31.0 41.0 34 36.812114312429 39.0 35.0 41.0 31.0 41.0 35 36.72170344865022 39.0 35.0 41.0 31.0 41.0 36 36.71981092353759 39.0 35.0 40.0 31.0 41.0 37 36.51228796505626 39.0 35.0 40.0 30.0 41.0 38 36.41245408670503 39.0 35.0 40.0 30.0 41.0 39 36.23229498744919 38.0 35.0 40.0 30.0 41.0 40 36.12332123975555 38.0 35.0 40.0 30.0 41.0 41 36.086674226986624 38.0 35.0 40.0 30.0 41.0 42 36.06361818525914 38.0 35.0 40.0 30.0 41.0 43 36.01110281399412 38.0 35.0 40.0 30.0 41.0 44 35.86596961103474 38.0 35.0 40.0 29.0 41.0 45 35.542802120606176 38.0 34.0 40.0 28.0 41.0 46 35.29314089238183 37.0 34.0 40.0 27.0 41.0 47 35.26247954043426 37.0 34.0 40.0 27.0 41.0 48 35.2214454881859 37.0 34.0 40.0 27.0 41.0 49 35.07064791355219 37.0 34.0 40.0 27.0 41.0 50 34.908779800546434 37.0 34.0 40.0 26.0 41.0 51 33.367797340390936 35.0 31.0 39.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 5.0 10 13.0 11 20.0 12 18.0 13 32.0 14 60.0 15 86.0 16 171.0 17 351.0 18 628.0 19 1299.0 20 2478.0 21 4252.0 22 6220.0 23 7948.0 24 9522.0 25 11800.0 26 14643.0 27 18421.0 28 23968.0 29 31641.0 30 41634.0 31 55317.0 32 72356.0 33 95619.0 34 142000.0 35 169060.0 36 193458.0 37 267281.0 38 393243.0 39 481218.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.253435519908923 36.955147154830556 18.36879006028206 25.422627264978452 2 21.56006664426934 35.20590624323007 18.24668062342809 24.9873464890725 3 19.406793627227323 26.98114859158477 27.355545807665653 26.25651197352225 4 26.07772458820463 27.471297925019815 17.615838919216564 28.83513856755899 5 18.32560919014107 35.96232945879161 17.65466747062307 28.05739388044425 6 21.203323215414837 33.03204529150022 23.562768994081335 22.201862499003614 7 78.345453807472 9.2002638776617 6.959602168726193 5.494680146140104 8 79.7485631235369 3.2988130884493865 5.168109533680834 11.78451425433288 9 76.80385273122671 6.325826317053217 9.80176410725874 7.068556844461332 10 39.83413264400429 28.374673025942265 14.65807157070293 17.133122759350517 11 28.454237324605224 25.671834189370855 24.04773466700116 21.826193819022762 12 29.050553893687038 21.10943049664847 30.527505920865067 19.31250968879943 13 26.476670838046306 25.124860200099075 24.771246528536782 23.627222433317833 14 20.41452657286197 24.175810203693405 31.501740682981506 23.907922540463115 15 20.191873682995688 26.91826003099438 26.430555820443868 26.459310465566066 16 22.479579556229755 25.25811939730655 28.874553948457788 23.387747098005903 17 21.348954734418086 25.64185698280638 27.559469056236356 25.44971922653917 18 22.38329061703654 25.647187350694683 25.580288788573647 26.389233243695127 19 23.865377402272106 28.40743767259511 26.50254023816475 21.224644686968034 20 25.414167139797943 28.65009166765694 24.312248060650784 21.62349313189433 21 24.010470994240755 25.134298374433406 25.43720019737032 25.41803043395552 22 23.799261279474123 26.827056947401008 24.92436012356673 24.449321649558144 23 25.42208933794386 25.476322163522973 22.369011099390825 26.73257739914235 24 21.91348470600087 29.75890599333851 24.103630176141763 24.22397912451886 25 23.81647494458129 25.08813445437327 24.192388136850592 26.90300246419484 26 27.186294401597742 25.96686271661955 21.591364217191465 25.255478664591248 27 22.121858078221436 25.3430629663155 25.593247939861712 26.941831015601352 28 25.001528201802834 27.174166592090423 23.243191433071853 24.58111377303489 29 23.408090520405285 28.50402002653447 22.689322197265668 25.398567255794575 30 25.724942258423084 26.577654413177843 22.021412430124503 25.675990898274577 31 28.644076665360974 27.535115632306333 20.081549738445208 23.739257963887493 32 24.430689813177942 27.287571391475424 20.829121609164712 27.452617186181925 33 24.999621005952896 26.635408215710697 21.792353318300716 26.57261746003569 34 21.813919302142367 26.479996205169282 25.142709597156227 26.563374895532128 35 22.163131752512484 28.54876577532157 22.828547494311422 26.45955497785452 36 23.455428099449993 27.708914966939492 24.128668234479463 24.706988699131053 37 23.691040140604347 29.86155225203153 21.16151161408919 25.28589599327493 38 26.68646238153991 25.239291951095588 23.795887009893455 24.27835865747105 39 22.32446096043449 25.561705854651134 26.609392108219183 25.50444107669519 40 23.04234903933567 24.865774979253132 24.96490026099242 27.126975720418784 41 21.327242043203366 23.304417310100753 30.70761367254034 24.66072697415554 42 24.352934905449544 24.468735925261395 23.044500747474068 28.133828421814993 43 23.39757649200176 22.970755841276315 26.729838861511663 26.901828805210265 44 23.87051216032964 22.359817437344947 24.851397656692033 28.918272745633377 45 27.74158180867696 23.58394375826146 24.62942940123342 24.045045031828163 46 20.751660116182457 23.647174636055684 30.4778210238512 25.12334422391066 47 22.704775373895966 26.171226087309467 24.53563448738243 26.58836405141213 48 22.433220026338862 22.369500123967732 29.92429410524885 25.272985744444558 49 23.329113051234614 23.145973347182508 26.89620502257582 26.628708579007053 50 22.251302883729025 22.78512211188198 25.475833138946065 29.487741865442928 51 20.86188625581756 22.608241922414297 29.560948844606084 26.968922977162062 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1057.0 1 990.0 2 923.0 3 768.5 4 614.0 5 646.0 6 678.0 7 719.0 8 760.0 9 838.0 10 916.0 11 1018.0 12 1120.0 13 1215.0 14 1310.0 15 1478.5 16 1647.0 17 1867.5 18 2088.0 19 2256.0 20 2424.0 21 2694.5 22 2965.0 23 4502.0 24 6039.0 25 7319.0 26 9883.0 27 11167.0 28 13911.5 29 16656.0 30 19515.0 31 22374.0 32 27665.5 33 32957.0 34 35018.5 35 37080.0 36 44290.5 37 51501.0 38 54394.0 39 57287.0 40 61983.5 41 66680.0 42 73451.5 43 80223.0 44 137958.5 45 195694.0 46 193746.5 47 191799.0 48 182814.5 49 173830.0 50 165679.5 51 157529.0 52 148034.5 53 138540.0 54 130540.0 55 122540.0 56 117243.0 57 111946.0 58 110101.0 59 108256.0 60 105883.0 61 103510.0 62 97582.5 63 91655.0 64 80975.0 65 70295.0 66 61323.5 67 52352.0 68 44409.5 69 36467.0 70 32546.5 71 28626.0 72 24306.0 73 19986.0 74 17141.5 75 11590.0 76 8883.0 77 7182.0 78 5481.0 79 4200.5 80 2920.0 81 2268.0 82 1616.0 83 1118.5 84 621.0 85 545.0 86 469.0 87 399.0 88 329.0 89 216.5 90 104.0 91 71.0 92 38.0 93 30.0 94 22.0 95 17.0 96 12.0 97 8.5 98 5.0 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2044887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.595626999327227 #Duplication Level Percentage of deduplicated Percentage of total 1 74.42192940134291 16.816101573230085 2 9.271768530526295 4.1900284667974494 3 3.2628456040289335 2.2117812667509695 4 1.6441109139195818 1.4859886782579945 5 1.0063079756899522 1.1369079832569107 6 0.7229315587590532 0.9801055108657067 7 0.588283937792065 0.9304851099631447 8 0.48500328353108235 0.8767162630493825 9 0.43250517556934376 0.8795453060199083 >10 6.596475544154198 36.04640405562935 >50 1.4585306728458869 21.748042126774692 >100 0.10010718532799012 3.627093974330574 >500 0.005038213804600027 0.77149957269508 >1k 0.0026286332893565357 1.3183352228609388 >5k 8.762110964521785E-4 1.7244032599681718 >10k+ 6.571583223391339E-4 5.256561629549637 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT 43421 2.1233936153929287 TruSeq Adapter, Index 19 (95% over 22bp) AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC 32919 1.6098200047239775 No Hit AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT 29861 1.4602762891054615 No Hit CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT 9369 0.4581671261052567 RNA PCR Primer, Index 40 (96% over 26bp) AACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG 9286 0.4541082221169189 RNA PCR Primer, Index 40 (95% over 24bp) AATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT 8639 0.4224683319909609 No Hit AATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT 7545 0.368969043277208 No Hit AATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC 4820 0.23570984606973394 TruSeq Adapter, Index 19 (95% over 21bp) AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCTC 4358 0.21311691061657684 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT 4302 0.21037837298589118 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 2160 0.10562930861216292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5609111897136614 0.0 2 0.0 0.0 0.0 0.7260547893355477 0.0 3 0.0 0.0 0.0 1.42002956642592 0.0 4 0.0 0.0 0.0 4.29784139661507 0.0 5 0.0 0.0 0.0 4.715761800040784 0.0 6 0.0 0.0 0.0 6.0481581622847616 0.0 7 0.0 0.0 0.0 7.134526259886243 0.0 8 0.0 0.0 0.0 7.937406810254063 0.0 9 0.0 0.0 0.0 10.383947866067905 0.0 10 4.890245769081617E-5 0.0 0.0 11.564502097181897 0.0 11 4.890245769081617E-5 0.0 0.0 14.24890470720387 0.0 12 4.890245769081617E-5 0.0 0.0 14.728099890116178 0.0 13 4.890245769081617E-5 0.0 0.0 15.056920015629226 0.0 14 4.890245769081617E-5 0.0 0.0 15.516358605634444 0.0 15 4.890245769081617E-5 0.0 0.0 15.755149306538698 0.0 16 4.890245769081617E-5 0.0 0.0 16.07228174466364 0.0 17 4.890245769081617E-5 0.0 0.0 16.41024662976487 0.0 18 4.890245769081617E-5 0.0 0.0 17.095370062013206 0.0 19 4.890245769081617E-5 0.0 0.0 17.33934442343269 0.0 20 4.890245769081617E-5 0.0 0.0 17.592365739524972 0.0 21 4.890245769081617E-5 0.0 0.0 17.899228661534842 0.0 22 4.890245769081617E-5 0.0 0.0 18.201934874640994 0.0 23 4.890245769081617E-5 0.0 0.0 18.507721942581668 0.0 24 4.890245769081617E-5 0.0 0.0 18.75159849908577 0.0 25 4.890245769081617E-5 0.0 0.0 18.974887120902036 0.0 26 4.890245769081617E-5 0.0 0.0 19.18795512906092 0.0 27 4.890245769081617E-5 0.0 0.0 19.411586068081025 0.0 28 4.890245769081617E-5 0.0 0.0 19.6284195654821 0.0 29 4.890245769081617E-5 0.0 0.0 19.86364038697493 0.0 30 4.890245769081617E-5 0.0 0.0 20.219307961760233 0.0 31 4.890245769081617E-5 0.0 0.0 20.44073829018425 0.0 32 4.890245769081617E-5 0.0 0.0 20.70026363314941 0.0 33 4.890245769081617E-5 0.0 0.0 20.94218409134588 0.0 34 4.890245769081617E-5 0.0 0.0 21.17349271622344 0.0 35 4.890245769081617E-5 0.0 0.0 21.416391223573722 0.0 36 4.890245769081617E-5 0.0 0.0 21.662028268554693 0.0 37 4.890245769081617E-5 0.0 0.0 21.91113738803171 0.0 38 4.890245769081617E-5 0.0 0.0 22.194135910688463 0.0 39 4.890245769081617E-5 0.0 0.0 22.468625405707016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTAG 35 1.2125565E-7 45.000004 1 TATCGCG 20 7.034576E-4 45.000004 1 ATTCACG 55 1.8189894E-12 45.0 1 GTATACG 30 2.1663363E-6 44.999996 1 AATCACG 125 0.0 41.399998 1 TATACGG 150 0.0 40.500004 2 CGATCGA 50 1.0822987E-9 40.5 18 CGATAGG 245 0.0 40.408165 2 TAACACG 135 0.0 40.0 1 CTACGCG 490 0.0 39.94898 1 TACGGCT 4165 0.0 39.813927 6 TACGCGG 555 0.0 39.72973 2 CTTAACG 80 0.0 39.375004 1 TAATGCG 40 3.4599907E-7 39.375004 1 ACGGCTG 4230 0.0 39.25532 7 TACGAAT 270 0.0 39.166664 11 CGTAACG 150 0.0 39.000004 1 TGATACC 4125 0.0 38.945454 3 AATCTGT 6105 0.0 38.660934 1 ACGGGAT 1270 0.0 38.622047 5 >>END_MODULE