FastQCFastQC Report
Sat 18 Jun 2016
SRR3553513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064638
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT436562.1144626806248845TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC335731.6260961970088705No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT304791.476239418241842No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT98840.47872799008833505RNA PCR Primer, Index 40 (96% over 26bp)
AACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG94780.45906352590623634RNA PCR Primer, Index 40 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT86640.4196377282603536No Hit
AATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT74380.3602568585873165No Hit
AATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC47970.23234097212198943TruSeq Adapter, Index 19 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT43180.20914077915838034No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCTC40050.19398073657464407No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG21330.10331108891728236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG5900.041.56782
ACTACCG900.040.01
CAATACG900.040.01
TACGGCT41600.039.6454356
ACGGCTG41650.039.5978367
CGAATAG403.460009E-739.3750041
TATAGCG403.460009E-739.3750041
CTACGCG5500.039.2727281
CGATGAA13200.038.86363618
AATCCCG1050.038.571431
AATACGG2450.038.571432
ACGAATA2800.038.5714312
TGATACC41100.038.5401463
GCGTACG659.094947E-1238.0769271
AATCTGT60950.037.9860531
GATACCT41850.037.9032254
ACGATAG950.037.8947371
AATCACG1250.037.8000031
GCTACGA3050.037.622959
TAACGGG9350.037.5401043
Produced by FastQC (version 0.11.5)