FastQCFastQC Report
Sat 18 Jun 2016
SRR3553512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849800
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT52280.28262514866472055Illumina PCR Primer Index 11 (96% over 25bp)
AATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT19020.1028219266947778Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA453.8562575E-1045.00000431
TTCGACG351.2124656E-745.0000041
CGAAACG406.8193913E-945.01
TATAGCG253.8917315E-545.01
TAAGCGT253.8917315E-545.036
ACGATAG302.1662217E-644.9999961
TATCGCG302.1662217E-644.9999961
TATACGG1600.042.18752
TATTGCG650.041.538461
ACGTTAG1150.041.086961
ACTACCG1700.041.029411
TAATACG556.184564E-1140.9090921
TTAATCG2200.040.90909220
TAATCGT2200.040.90909221
TATATCG501.0822987E-940.51
TCTACGG1900.040.2631572
AATTACG850.039.7058831
ATCGTTG2300.039.13043623
ATTCGCG356.250353E-638.571431
TATACGA356.250353E-638.5714331
Produced by FastQC (version 0.11.5)