FastQCFastQC Report
Sat 18 Jun 2016
SRR3553509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876396
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT47730.25437061259989896TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT19140.10200405458122913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA502.1827873E-1145.031
TTCGACG207.03443E-445.01
TATTACG800.042.18751
TATCGCG800.042.18751
ATTACCG750.042.01
ATACCGG2600.041.538462
TATAGCG556.184564E-1140.9090921
CTTACCG1000.040.51
CTAACGG4200.039.6428572
CCTAACG800.039.3751
CATACCG800.039.3751
CGAATAG750.039.01
CGGCACG356.2504023E-638.571431
TACGCCG356.2504023E-638.571431
TCATACG700.038.571431
CGGGATA14100.038.4574476
TAATACG659.094947E-1238.076921
TAACGGG11850.037.974683
TTCGGGA4450.037.921354
CCGCACG1250.037.8000031
Produced by FastQC (version 0.11.5)