##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553509_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1876396 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.030760031464574 33.0 31.0 34.0 30.0 34.0 2 32.22838782431853 34.0 31.0 34.0 30.0 34.0 3 32.36260736006685 34.0 31.0 34.0 30.0 34.0 4 35.92393236822078 37.0 35.0 37.0 35.0 37.0 5 35.76164626230284 37.0 35.0 37.0 35.0 37.0 6 35.77348118414237 37.0 35.0 37.0 35.0 37.0 7 35.89354166178141 37.0 35.0 37.0 35.0 37.0 8 36.02963287067335 37.0 35.0 37.0 35.0 37.0 9 37.640059454400884 39.0 37.0 39.0 35.0 39.0 10 37.20724516573261 39.0 37.0 39.0 34.0 39.0 11 37.175224206404195 39.0 37.0 39.0 34.0 39.0 12 37.13159429033104 39.0 37.0 39.0 33.0 39.0 13 37.102324882380906 39.0 37.0 39.0 33.0 39.0 14 38.35977640114347 40.0 38.0 41.0 34.0 41.0 15 38.34905958017391 40.0 38.0 41.0 34.0 41.0 16 38.29463556733227 40.0 38.0 41.0 33.0 41.0 17 38.271430444319854 40.0 38.0 41.0 33.0 41.0 18 38.216990976318435 40.0 37.0 41.0 33.0 41.0 19 38.23482090134492 40.0 37.0 41.0 34.0 41.0 20 38.16459318821826 40.0 37.0 41.0 34.0 41.0 21 38.15245342667539 40.0 37.0 41.0 33.0 41.0 22 38.12252797383921 40.0 37.0 41.0 33.0 41.0 23 38.016854651150396 40.0 37.0 41.0 33.0 41.0 24 37.94678362136777 40.0 37.0 41.0 33.0 41.0 25 37.938228923958484 40.0 37.0 41.0 33.0 41.0 26 37.88161027842737 40.0 37.0 41.0 33.0 41.0 27 37.82612518892601 40.0 37.0 41.0 33.0 41.0 28 37.76315074216743 40.0 37.0 41.0 33.0 41.0 29 37.64844361211599 40.0 37.0 41.0 33.0 41.0 30 37.64118608225556 40.0 37.0 41.0 33.0 41.0 31 37.59362629210465 40.0 36.0 41.0 33.0 41.0 32 37.5457989678085 40.0 36.0 41.0 33.0 41.0 33 37.510757857083476 40.0 36.0 41.0 32.0 41.0 34 37.42252434987071 40.0 36.0 41.0 32.0 41.0 35 37.3643218169299 40.0 36.0 41.0 32.0 41.0 36 37.352661165340365 39.0 36.0 41.0 32.0 41.0 37 37.246002442981116 39.0 36.0 41.0 32.0 41.0 38 37.189193006167145 39.0 35.0 41.0 32.0 41.0 39 37.12692043683742 39.0 35.0 41.0 31.0 41.0 40 37.03855103080586 39.0 35.0 41.0 31.0 41.0 41 36.98204856544141 39.0 35.0 41.0 31.0 41.0 42 36.889341588875695 39.0 35.0 41.0 31.0 41.0 43 36.81582619020718 39.0 35.0 41.0 31.0 41.0 44 36.72918509738882 39.0 35.0 40.0 31.0 41.0 45 36.56043606999802 39.0 35.0 40.0 31.0 41.0 46 36.43450369751375 39.0 35.0 40.0 30.0 41.0 47 36.31725819070175 38.0 35.0 40.0 30.0 41.0 48 36.25812674936421 38.0 35.0 40.0 30.0 41.0 49 36.097767209053956 38.0 35.0 40.0 30.0 41.0 50 35.92543205165647 38.0 35.0 40.0 29.0 41.0 51 34.695645801845664 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 12.0 10 13.0 11 14.0 12 13.0 13 19.0 14 40.0 15 66.0 16 127.0 17 223.0 18 417.0 19 714.0 20 1214.0 21 1995.0 22 2918.0 23 4161.0 24 5562.0 25 7620.0 26 9802.0 27 12958.0 28 17029.0 29 22446.0 30 29592.0 31 38284.0 32 50779.0 33 68534.0 34 105333.0 35 131982.0 36 163086.0 37 242194.0 38 398938.0 39 560120.0 40 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.346504682380477 31.77692768477443 20.84986324848273 27.02670438436236 2 23.319544488476847 29.187282428655788 20.306694322520404 27.18647876034696 3 20.442593141319847 30.156320947177463 21.31943363767563 28.08165227382706 4 21.214764900372842 31.408402064382994 19.401714776624974 27.97511825861918 5 19.26784111669392 33.243942110300814 17.365364240810575 30.1228525321947 6 21.170744341812707 36.156440324963384 18.655497027279957 24.017318305943945 7 88.3784126591615 3.5432286148552863 4.13857202850571 3.939786697477505 8 89.55359103302288 3.251339269535855 3.395924954007576 3.799144743433689 9 81.49377849878171 7.793184381122109 6.618219181878453 4.094817938217733 10 33.817062070053446 40.24496961195824 11.880860969646067 14.05710734834225 11 24.444360358900784 26.763646906090187 29.818545765392805 18.973446969616223 12 26.395654222243063 23.10668963267882 29.97336383151531 20.52429231356281 13 24.977669958793346 23.73427570726009 29.804582827931842 21.483471506014723 14 21.24002609257321 26.10312535307046 29.43872189026197 23.21812666409436 15 20.9492026203424 27.91025988117647 29.69405178864163 21.4464857098395 16 23.594806213613758 26.98545509583265 28.081439099209337 21.33829959134426 17 22.910249222445582 26.8505688564674 28.515569208205516 21.7236127128815 18 23.543111368815538 25.97884455093701 27.673209706266693 22.80483437398076 19 23.13376280912984 28.438613171206935 26.928910528481193 21.49871349118203 20 24.897569596183324 28.001605204871467 25.972182844133112 21.128642354812097 21 25.146077906795796 27.525159934256948 25.978471495355993 21.350290663591267 22 23.605944587389867 25.559849839799277 26.323228145871123 24.51097742693973 23 22.517901338523423 28.35739364185385 25.911268197118304 23.21343682250442 24 21.506227896456824 27.198363245285112 28.192769543316015 23.102639314942046 25 23.23672614949083 28.403652533900093 25.1972398150497 23.162381501559373 26 24.199102961208617 27.921931191496892 25.278565931711643 22.60039991558285 27 22.660568451435626 26.678057297073753 25.496590271989493 25.164783979501127 28 23.089049433062105 28.170919144999246 26.884090565104596 21.855940856834057 29 24.338572454854944 26.930402750805264 25.507568764802315 23.223456029537477 30 24.15327041839782 25.992381139162525 26.14602674488754 23.708321697552115 31 25.316830775593214 26.63675471488961 24.224524034372273 23.821890475144905 32 24.572318423189987 26.39171049181516 25.458858364652237 23.577112720342612 33 23.772487257487228 26.489824109622916 24.93812606720543 24.79956256568443 34 22.29454763280246 26.81736690975679 27.686586413528914 23.201499043911838 35 23.885469804881275 25.87795966309883 27.05612248160836 23.180448050411535 36 23.65678673371719 27.574882913841215 26.219625281656967 22.54870507078463 37 23.365856674177518 28.105154775431203 25.123428103662555 23.405560446728728 38 23.022538952332024 26.57269574226336 27.76668677613894 22.638078529265677 39 22.02264340789471 25.372682525437064 27.302712220661313 25.30196184600692 40 22.739709528265887 25.817311484356182 28.589487506901527 22.853491480476404 41 21.377630308314448 26.751122897298863 28.53789924941217 23.333347544974515 42 23.22308297395646 27.633399346406623 26.628813960379365 22.514703719257557 43 22.76065393445733 25.84811521661739 27.318700316990657 24.072530531934625 44 24.03133453705934 24.78991641423239 26.910470924048013 24.268278124660252 45 23.89975250426882 25.126945484855007 27.493716678142565 23.479585332733603 46 21.710236005619283 25.371296890421853 28.12604588796821 24.792421215990654 47 22.082225713548738 26.827759172370865 27.21642979413727 23.873585319943125 48 22.689027262901863 25.14415933523627 28.673371718976163 23.493441682885702 49 22.902468348898633 25.898371132745968 26.934932711431912 24.264227806923486 50 21.774028509973377 25.233852555643903 27.435200245577157 25.556918688805563 51 21.74839426219199 24.171923197448727 28.597641436029498 25.482041104329788 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1154.0 1 978.0 2 802.0 3 631.5 4 461.0 5 485.0 6 509.0 7 580.5 8 652.0 9 774.0 10 896.0 11 1087.5 12 1279.0 13 1565.0 14 1851.0 15 1879.5 16 1908.0 17 1903.5 18 1899.0 19 2579.5 20 3260.0 21 3784.0 22 4308.0 23 6490.0 24 8672.0 25 9662.0 26 13994.0 27 17336.0 28 21243.5 29 25151.0 30 29235.0 31 33319.0 32 38969.5 33 44620.0 34 50120.0 35 55620.0 36 61036.5 37 66453.0 38 71952.5 39 77452.0 40 83191.0 41 88930.0 42 93570.5 43 98211.0 44 110227.0 45 122243.0 46 134854.0 47 147465.0 48 157755.0 49 168045.0 50 165738.0 51 163431.0 52 151463.5 53 139496.0 54 125492.0 55 111488.0 56 104000.5 57 96513.0 58 91352.0 59 86191.0 60 82048.5 61 77906.0 62 71684.0 63 65462.0 64 55389.5 65 45317.0 66 38680.0 67 32043.0 68 27844.5 69 23646.0 70 20185.0 71 16724.0 72 14477.5 73 12231.0 74 11004.5 75 7991.5 76 6205.0 77 4691.0 78 3177.0 79 2563.5 80 1950.0 81 1423.5 82 897.0 83 666.0 84 435.0 85 311.5 86 188.0 87 135.5 88 83.0 89 61.0 90 39.0 91 32.5 92 26.0 93 20.5 94 15.0 95 10.0 96 5.0 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1876396.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.366052900874116 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98076346067762 17.052723797683743 2 10.46538621413194 4.890695358149713 3 3.7277407201013046 2.61307760599889 4 1.8387594831660734 1.7185820542256969 5 1.0998860601441514 1.2849997933131125 6 0.8005545641144113 1.1223480177080132 7 0.5794200350012856 0.9477131432766461 8 0.4995790590217435 0.9338552577016777 9 0.43449081034113646 0.9137101733437191 >10 5.713225050208079 32.02148362501978 >50 1.5250727613631692 25.21059511703825 >100 0.32981703346350005 10.076681594795836 >500 0.003920900891968249 0.580036922096087 >1k 0.0013838473736358526 0.6334975396487506 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT 4773 0.25437061259989896 TruSeq Adapter, Index 13 (95% over 22bp) AATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT 1914 0.10200405458122913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.329365443115419E-5 0.0 0.0 0.29220910724601845 0.0 2 5.329365443115419E-5 0.0 0.0 0.44670741144193443 0.0 3 5.329365443115419E-5 0.0 0.0 0.6077608351328824 0.0 4 5.329365443115419E-5 0.0 0.0 1.2680692135348828 0.0 5 5.329365443115419E-5 0.0 0.0 1.4321603755284067 0.0 6 5.329365443115419E-5 0.0 0.0 2.465364453985193 0.0 7 5.329365443115419E-5 0.0 0.0 3.27974478734766 0.0 8 5.329365443115419E-5 0.0 0.0 4.226506558317114 0.0 9 1.0658730886230838E-4 0.0 0.0 5.150192176917879 0.0 10 1.0658730886230838E-4 0.0 0.0 6.587841798852694 0.0 11 1.0658730886230838E-4 0.0 0.0 7.636021394204635 0.0 12 1.0658730886230838E-4 0.0 0.0 8.181322066344205 0.0 13 1.0658730886230838E-4 0.0 0.0 8.453013116634228 0.0 14 1.0658730886230838E-4 0.0 0.0 8.710368173882273 0.0 15 1.0658730886230838E-4 0.0 0.0 8.92450207738665 0.0 16 1.0658730886230838E-4 0.0 0.0 9.2860462290476 0.0 17 1.0658730886230838E-4 0.0 0.0 9.763823841022898 0.0 18 1.0658730886230838E-4 0.0 0.0 10.322980863314568 0.0 19 1.0658730886230838E-4 0.0 0.0 10.617641478664419 0.0 20 1.0658730886230838E-4 0.0 0.0 10.944864516871705 0.0 21 1.0658730886230838E-4 0.0 0.0 11.302411644450318 0.0 22 1.0658730886230838E-4 0.0 0.0 11.731692030893266 0.0 23 1.0658730886230838E-4 0.0 0.0 12.139388487291596 0.0 24 1.0658730886230838E-4 0.0 0.0 12.458404302716485 0.0 25 1.0658730886230838E-4 0.0 0.0 12.733772615162257 0.0 26 1.0658730886230838E-4 0.0 0.0 13.001519935024376 0.0 27 1.0658730886230838E-4 0.0 0.0 13.297992534624887 0.0 28 1.0658730886230838E-4 0.0 0.0 13.587004022605036 0.0 29 1.0658730886230838E-4 0.0 0.0 13.922647458212445 0.0 30 1.0658730886230838E-4 0.0 0.0 14.410124515294212 0.0 31 1.0658730886230838E-4 0.0 0.0 14.733403823073594 0.0 32 1.0658730886230838E-4 0.0 0.0 15.08226408497993 0.0 33 1.5988096329346257E-4 0.0 0.0 15.408900892988473 0.0 34 1.5988096329346257E-4 0.0 0.0 15.710436389759945 0.0 35 1.5988096329346257E-4 0.0 0.0 16.04448101573442 0.0 36 1.5988096329346257E-4 0.0 0.0 16.353051274890802 0.0 37 1.5988096329346257E-4 0.0 0.0 16.673559312639764 0.0 38 1.5988096329346257E-4 0.0 0.0 16.996252390220402 0.0 39 1.5988096329346257E-4 0.0 0.0 17.334986857784816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTCTA 50 2.1827873E-11 45.0 31 TTCGACG 20 7.03443E-4 45.0 1 TATTACG 80 0.0 42.1875 1 TATCGCG 80 0.0 42.1875 1 ATTACCG 75 0.0 42.0 1 ATACCGG 260 0.0 41.53846 2 TATAGCG 55 6.184564E-11 40.909092 1 CTTACCG 100 0.0 40.5 1 CTAACGG 420 0.0 39.642857 2 CCTAACG 80 0.0 39.375 1 CATACCG 80 0.0 39.375 1 CGAATAG 75 0.0 39.0 1 CGGCACG 35 6.2504023E-6 38.57143 1 TACGCCG 35 6.2504023E-6 38.57143 1 TCATACG 70 0.0 38.57143 1 CGGGATA 1410 0.0 38.457447 6 TAATACG 65 9.094947E-12 38.07692 1 TAACGGG 1185 0.0 37.97468 3 TTCGGGA 445 0.0 37.92135 4 CCGCACG 125 0.0 37.800003 1 >>END_MODULE