FastQCFastQC Report
Sat 18 Jun 2016
SRR3553503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409456
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT46821.1434684068617873Illumina PCR Primer Index 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15000.3663397288109101No Hit
AAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT11200.27353366417881286No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT10360.25301863936540187No Hit
AATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT9160.22371146106052908No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC8820.21540776054081515No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT8000.19538118869915203No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC6970.17022586065413622TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT5870.1433609472080028No Hit
GCTGCCGGGGTGAACAAAGTGTGCTCACTGGGTTTCTGGATTATTCCCATA5140.12553241373920518No Hit
ACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG4770.11649603376186941No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.02796E-445.00000418
ATCCTAC207.02796E-445.00000434
GTCATAC207.02796E-445.0000048
TTCTACG207.02796E-445.00000435
CCTTATG207.02796E-445.00000410
GTCGCGG207.02796E-445.0000042
ACGTTTA207.02796E-445.00000429
CAAAACG207.02796E-445.0000041
CGTGCAA207.02796E-445.00000411
GTCCTAG207.02796E-445.00000427
TTAGACT406.7957444E-945.00000428
CGTTGGA207.02796E-445.00000429
GGCCGAT406.7957444E-945.0000048
TCTTACG207.02796E-445.0000041
CCAATCG207.02796E-445.00000430
CGACACT207.02796E-445.00000435
TCGATAG207.02796E-445.0000041
CCATGCG207.02796E-445.0000041
ACCTCGT207.02796E-445.0000044
CCCACGT207.02796E-445.00000422