Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553503_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 409456 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT | 4682 | 1.1434684068617873 | Illumina PCR Primer Index 1 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1500 | 0.3663397288109101 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT | 1120 | 0.27353366417881286 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT | 1036 | 0.25301863936540187 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT | 916 | 0.22371146106052908 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC | 882 | 0.21540776054081515 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT | 800 | 0.19538118869915203 | No Hit |
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC | 697 | 0.17022586065413622 | TruSeq Adapter, Index 13 (95% over 22bp) |
AAAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT | 587 | 0.1433609472080028 | No Hit |
GCTGCCGGGGTGAACAAAGTGTGCTCACTGGGTTTCTGGATTATTCCCATA | 514 | 0.12553241373920518 | No Hit |
ACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG | 477 | 0.11649603376186941 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGCGAT | 20 | 7.02796E-4 | 45.000004 | 18 |
ATCCTAC | 20 | 7.02796E-4 | 45.000004 | 34 |
GTCATAC | 20 | 7.02796E-4 | 45.000004 | 8 |
TTCTACG | 20 | 7.02796E-4 | 45.000004 | 35 |
CCTTATG | 20 | 7.02796E-4 | 45.000004 | 10 |
GTCGCGG | 20 | 7.02796E-4 | 45.000004 | 2 |
ACGTTTA | 20 | 7.02796E-4 | 45.000004 | 29 |
CAAAACG | 20 | 7.02796E-4 | 45.000004 | 1 |
CGTGCAA | 20 | 7.02796E-4 | 45.000004 | 11 |
GTCCTAG | 20 | 7.02796E-4 | 45.000004 | 27 |
TTAGACT | 40 | 6.7957444E-9 | 45.000004 | 28 |
CGTTGGA | 20 | 7.02796E-4 | 45.000004 | 29 |
GGCCGAT | 40 | 6.7957444E-9 | 45.000004 | 8 |
TCTTACG | 20 | 7.02796E-4 | 45.000004 | 1 |
CCAATCG | 20 | 7.02796E-4 | 45.000004 | 30 |
CGACACT | 20 | 7.02796E-4 | 45.000004 | 35 |
TCGATAG | 20 | 7.02796E-4 | 45.000004 | 1 |
CCATGCG | 20 | 7.02796E-4 | 45.000004 | 1 |
ACCTCGT | 20 | 7.02796E-4 | 45.000004 | 4 |
CCCACGT | 20 | 7.02796E-4 | 45.000004 | 22 |