##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553503_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409456 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.670015825876284 31.0 31.0 34.0 30.0 34.0 2 31.865304208510803 33.0 31.0 34.0 30.0 34.0 3 32.0364556875464 33.0 31.0 34.0 30.0 34.0 4 35.665360869055526 37.0 35.0 37.0 33.0 37.0 5 35.474221894416004 37.0 35.0 37.0 33.0 37.0 6 35.4819052596616 37.0 35.0 37.0 33.0 37.0 7 35.57283078035247 37.0 35.0 37.0 35.0 37.0 8 35.77979318901176 37.0 35.0 37.0 35.0 37.0 9 37.37537122425853 39.0 37.0 39.0 35.0 39.0 10 36.89839689734672 39.0 37.0 39.0 33.0 39.0 11 36.889326325661365 39.0 37.0 39.0 33.0 39.0 12 36.83984115509359 39.0 37.0 39.0 33.0 39.0 13 36.74037014966199 39.0 35.0 39.0 32.0 39.0 14 37.89213737251377 40.0 37.0 41.0 33.0 41.0 15 37.89310939392755 40.0 37.0 41.0 33.0 41.0 16 37.83694218670627 40.0 37.0 41.0 33.0 41.0 17 37.83104167480755 39.0 37.0 41.0 33.0 41.0 18 37.751709585401116 39.0 37.0 41.0 33.0 41.0 19 37.687565941151185 39.0 36.0 41.0 32.0 41.0 20 37.603713219491226 39.0 36.0 41.0 32.0 41.0 21 37.565606560900314 39.0 36.0 41.0 32.0 41.0 22 37.5727990309093 39.0 36.0 41.0 32.0 41.0 23 37.40900853815795 39.0 36.0 41.0 32.0 41.0 24 37.30551268023915 39.0 36.0 41.0 32.0 41.0 25 37.291445234652805 39.0 36.0 41.0 32.0 41.0 26 37.2068793716541 39.0 36.0 41.0 32.0 41.0 27 37.09756359657692 39.0 35.0 41.0 31.0 41.0 28 37.01674661013637 39.0 35.0 40.0 31.0 41.0 29 36.809867238482276 39.0 35.0 40.0 31.0 41.0 30 36.759031495447616 39.0 35.0 40.0 31.0 41.0 31 36.68443251533742 39.0 35.0 40.0 30.0 41.0 32 36.499763100308705 39.0 35.0 40.0 30.0 41.0 33 36.459258137626506 39.0 35.0 40.0 30.0 41.0 34 36.379896741041776 39.0 35.0 40.0 30.0 41.0 35 36.3029287640186 39.0 35.0 40.0 30.0 41.0 36 36.23075251064827 39.0 35.0 40.0 30.0 41.0 37 36.06022136688679 38.0 35.0 40.0 29.0 41.0 38 36.00175354616857 38.0 35.0 40.0 29.0 41.0 39 35.9087569848775 38.0 35.0 40.0 29.0 41.0 40 35.84628873432066 38.0 35.0 40.0 29.0 41.0 41 35.773743698956665 38.0 35.0 40.0 28.0 41.0 42 35.61289857762495 38.0 34.0 40.0 28.0 41.0 43 35.47104695009964 38.0 34.0 40.0 27.0 41.0 44 35.36283752100348 38.0 34.0 40.0 27.0 41.0 45 35.13156480794029 38.0 34.0 40.0 26.0 41.0 46 34.945146731272715 38.0 34.0 40.0 26.0 41.0 47 34.871041088663986 38.0 34.0 40.0 26.0 41.0 48 34.785346899300535 37.0 33.0 40.0 26.0 41.0 49 34.60491237153687 37.0 33.0 40.0 24.0 41.0 50 34.35735952092533 37.0 33.0 40.0 24.0 41.0 51 32.898584951740844 35.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 3.0 11 2.0 12 6.0 13 7.0 14 10.0 15 19.0 16 41.0 17 84.0 18 192.0 19 384.0 20 622.0 21 987.0 22 1431.0 23 1982.0 24 2596.0 25 3107.0 26 3679.0 27 4473.0 28 5702.0 29 6986.0 30 8972.0 31 11426.0 32 14882.0 33 18720.0 34 27172.0 35 34358.0 36 40226.0 37 55222.0 38 79222.0 39 86909.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.95135008401391 32.01320776835606 21.650189519753038 26.385252627876987 2 21.462135125630105 31.76702747059513 21.740553319526395 25.030284084248365 3 20.82104060021101 31.611699425579303 21.975499198937126 25.591760775272554 4 19.73325583212848 32.614737603063574 20.756809034426166 26.895197530381775 5 17.512015943105 35.19865382360987 18.654507444023288 28.63482278926185 6 20.701613848618656 37.358837091164865 19.25261810792857 22.686930952287916 7 81.30763745066625 5.837989918330663 6.866671876831698 5.987700754171389 8 82.03860732288695 4.975137743738032 5.89733695439803 7.088917978976984 9 74.25535344457036 8.355232308233363 11.727023172208979 5.6623910749873 10 33.76773084287445 30.548581532570047 15.636356531593137 20.04733109296237 11 23.134353874408973 29.382888515493725 28.838996131452465 18.643761478644834 12 25.56733812668516 23.70266890703763 30.663367980930794 20.066624985346408 13 25.299177445195575 24.260237974287836 29.092258997303738 21.348325583212848 14 20.1154702825212 26.75379039506076 31.035080692430935 22.095658629987106 15 18.28694658278301 29.478625297956313 30.12313899417764 22.111289125083037 16 20.173351959673322 29.3831327419796 27.257873861904574 23.1856414364425 17 19.52615177210738 29.09128209136024 29.088595600015633 22.293970536516746 18 21.221816263530147 25.88776327615177 29.32940291508734 23.561017545230744 19 19.91154116681646 32.164628189597906 28.40964596928608 19.51418467429956 20 22.060245789535387 28.654116681645892 27.51089250126998 21.774745027548747 21 20.392667343988123 31.604372631003088 25.521179320854987 22.481780704153802 22 19.172511820561915 27.65913797819546 27.834981048024698 25.333369153217927 23 21.631384080340744 29.33282208588957 24.41678715173303 24.619006682036655 24 17.949914032276972 29.424651244578172 26.113428549099293 26.51200617404556 25 21.75203196436247 28.098745652768553 25.867736704310108 24.281485678558866 26 22.538197022390687 27.869416982532925 25.83378922277363 23.758596772302763 27 19.501973350005862 28.289486538236098 25.288919932788872 26.91962017896917 28 23.727091555625023 26.641201985072875 25.808633894728615 23.823072564573483 29 22.69157125551952 29.346742995584385 22.64199327888711 25.319692470008988 30 24.349624868117697 29.057578836309638 22.05560548630378 24.537190809268882 31 23.069633855652377 27.33333984603962 21.565198702668905 28.031827595639093 32 23.020544331991715 30.42353757180259 21.269196201789693 25.286721894416004 33 20.997616349497868 26.47195303036224 23.099429486928997 29.431001133210895 34 21.497303739595953 27.97223633308585 24.539144621155877 25.99131530616232 35 21.281895979055136 28.487798444765737 22.072212887343206 28.158092688835918 36 19.48634285490993 28.75864561759994 26.332255480442342 25.42275604704779 37 19.407946152944394 32.09648900003907 22.317416279160643 26.178148567855885 38 21.092864678988708 30.173205423781795 25.47990504474229 23.254024852487202 39 19.952326989957406 26.831942870540427 26.16911218787855 27.04661795162362 40 22.193593450822554 27.496483138603416 25.320913602438356 24.989009808135673 41 20.43174358172795 25.094271423547344 28.9970106678129 25.476974326911805 42 24.425090852252744 26.512983079989063 25.595424172560666 23.46650189519753 43 19.475596889531477 25.858211871361025 27.623480911257865 27.042710327849633 44 22.626118557305304 25.472089797194325 24.619006682036655 27.28278496346372 45 23.590568950021492 24.144474619983587 27.774901332499706 24.490055097495215 46 20.10179359931226 24.726222109335314 30.103845101793596 25.06813918955883 47 20.530655308506898 24.87690985111953 27.10913993200735 27.483294908366222 48 22.38799773357821 22.83737446758626 31.02384627408073 23.750781524754796 49 22.809288421710757 23.43108905474581 26.609940994881015 27.149681528662423 50 20.48986948536595 21.3993689187605 27.64497284201477 30.46578875385878 51 21.385936462037435 21.77865265132273 30.487280684615687 26.34813020202415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1837.0 1 1504.5 2 1172.0 3 820.0 4 468.0 5 465.5 6 463.0 7 490.0 8 517.0 9 562.5 10 608.0 11 686.0 12 764.0 13 848.0 14 932.0 15 921.5 16 911.0 17 981.0 18 1051.0 19 964.5 20 878.0 21 1083.5 22 1289.0 23 1464.5 24 1640.0 25 1861.0 26 2473.0 27 2864.0 28 3986.5 29 5109.0 30 5445.5 31 5782.0 32 5947.5 33 6113.0 34 6615.5 35 7118.0 36 9229.0 37 11340.0 38 12298.0 39 13256.0 40 14024.0 41 14792.0 42 17402.0 43 20012.0 44 25594.5 45 31177.0 46 35980.0 47 40783.0 48 46051.5 49 51320.0 50 47228.0 51 43136.0 52 38147.0 53 33158.0 54 28551.5 55 23945.0 56 21536.0 57 19127.0 58 17466.0 59 15805.0 60 14221.0 61 12637.0 62 11358.5 63 10080.0 64 8699.0 65 7318.0 66 6688.0 67 6058.0 68 5026.0 69 3994.0 70 3625.0 71 3256.0 72 2790.5 73 2325.0 74 1924.5 75 1329.0 76 1134.0 77 990.5 78 847.0 79 664.0 80 481.0 81 307.0 82 133.0 83 125.0 84 117.0 85 91.0 86 65.0 87 42.5 88 20.0 89 13.0 90 6.0 91 5.0 92 4.0 93 5.5 94 7.0 95 4.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 409456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.6093950508874 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96286343145172 24.771030228328172 2 10.432011748266106 6.803631845494159 3 4.071329741173716 3.982907997370825 4 2.0497938244042078 2.6737014637146452 5 1.1836309594549876 1.9298744775664285 6 0.8380087323335761 1.6396174685255365 7 0.5372973413063074 1.2264658883714197 8 0.4051416370416316 1.056913895508304 9 0.3308445321984899 0.9709776045797934 >10 3.1163028005374835 23.886488796143095 >50 0.9069827129123323 20.664168995869264 >100 0.15829061408147405 7.279061778999525 >500 0.004501154902790731 1.0754076820864364 >1k 0.003000769935193821 2.039751877442381 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT 4682 1.1434684068617873 Illumina PCR Primer Index 1 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1500 0.3663397288109101 No Hit AAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT 1120 0.27353366417881286 No Hit AAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT 1036 0.25301863936540187 No Hit AATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT 916 0.22371146106052908 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC 882 0.21540776054081515 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT 800 0.19538118869915203 No Hit CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC 697 0.17022586065413622 TruSeq Adapter, Index 13 (95% over 22bp) AAAAAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT 587 0.1433609472080028 No Hit GCTGCCGGGGTGAACAAAGTGTGCTCACTGGGTTTCTGGATTATTCCCATA 514 0.12553241373920518 No Hit ACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG 477 0.11649603376186941 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.4422648587394004E-4 0.0 0.0 1.3473975225665273 0.0 2 2.4422648587394004E-4 0.0 0.0 1.79262240631472 0.0 3 2.4422648587394004E-4 0.0 0.0 2.31453440662733 0.0 4 2.4422648587394004E-4 0.0 0.0 4.559220038294713 0.0 5 2.4422648587394004E-4 0.0 0.0 4.905044742292212 0.0 6 2.4422648587394004E-4 0.0 0.0 7.093558282208589 0.0 7 2.4422648587394004E-4 0.0 0.0 8.896682427415888 0.0 8 2.4422648587394004E-4 0.0 0.0 10.907154859130163 0.0 9 2.4422648587394004E-4 0.0 0.0 13.043892384041264 0.0 10 2.4422648587394004E-4 0.0 0.0 16.393458637802354 0.0 11 2.4422648587394004E-4 0.0 0.0 18.830350513852526 0.0 12 2.4422648587394004E-4 0.0 0.0 20.041225430815523 0.0 13 2.4422648587394004E-4 0.0 0.0 20.577791020280568 0.0 14 2.4422648587394004E-4 0.0 0.0 21.087003243327732 0.0 15 2.4422648587394004E-4 0.0 0.0 21.498769098511197 0.0 16 2.4422648587394004E-4 0.0 0.0 22.338175530459928 0.0 17 2.4422648587394004E-4 0.0 0.0 23.378091907311163 0.0 18 2.4422648587394004E-4 0.0 0.0 24.903774764565668 0.0 19 2.4422648587394004E-4 0.0 0.0 25.485033800945644 0.0 20 2.4422648587394004E-4 0.0 0.0 26.165448790590442 0.0 21 2.4422648587394004E-4 0.0 0.0 26.811672072212886 0.0 22 2.4422648587394004E-4 0.0 0.0 27.49184283537181 0.0 23 2.4422648587394004E-4 0.0 0.0 28.128785510531046 0.0 24 2.4422648587394004E-4 0.0 0.0 28.55667031378219 0.0 25 2.4422648587394004E-4 0.0 0.0 28.959155562502442 0.0 26 2.4422648587394004E-4 0.0 0.0 29.311574381618538 0.0 27 2.4422648587394004E-4 0.0 0.0 29.760462662654838 0.0 28 2.4422648587394004E-4 0.0 0.0 30.20544331991716 0.0 29 2.4422648587394004E-4 0.0 0.0 30.631130084795437 0.0 30 2.4422648587394004E-4 0.0 0.0 31.281993669649488 0.0 31 2.4422648587394004E-4 0.0 0.0 31.68643273025673 0.0 32 2.4422648587394004E-4 0.0 0.0 32.08745262006174 0.0 33 2.4422648587394004E-4 0.0 0.0 32.49018209526787 0.0 34 2.4422648587394004E-4 0.0 0.0 32.878013754835685 0.0 35 2.4422648587394004E-4 0.0 0.0 33.290512289476766 0.0 36 2.4422648587394004E-4 0.0 0.0 33.684205384705564 0.0 37 2.4422648587394004E-4 0.0 0.0 34.08034074479309 0.0 38 2.4422648587394004E-4 0.0 0.0 34.49235082646243 0.0 39 2.4422648587394004E-4 0.0 0.0 34.89752256652729 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAT 20 7.02796E-4 45.000004 18 ATCCTAC 20 7.02796E-4 45.000004 34 GTCATAC 20 7.02796E-4 45.000004 8 TTCTACG 20 7.02796E-4 45.000004 35 CCTTATG 20 7.02796E-4 45.000004 10 GTCGCGG 20 7.02796E-4 45.000004 2 ACGTTTA 20 7.02796E-4 45.000004 29 CAAAACG 20 7.02796E-4 45.000004 1 CGTGCAA 20 7.02796E-4 45.000004 11 GTCCTAG 20 7.02796E-4 45.000004 27 TTAGACT 40 6.7957444E-9 45.000004 28 CGTTGGA 20 7.02796E-4 45.000004 29 GGCCGAT 40 6.7957444E-9 45.000004 8 TCTTACG 20 7.02796E-4 45.000004 1 CCAATCG 20 7.02796E-4 45.000004 30 CGACACT 20 7.02796E-4 45.000004 35 TCGATAG 20 7.02796E-4 45.000004 1 CCATGCG 20 7.02796E-4 45.000004 1 ACCTCGT 20 7.02796E-4 45.000004 4 CCCACGT 20 7.02796E-4 45.000004 22 >>END_MODULE