##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945228
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05026406327362	33.0	31.0	34.0	30.0	34.0
2	32.24263669717782	34.0	31.0	34.0	30.0	34.0
3	32.37611983563754	34.0	31.0	34.0	30.0	34.0
4	35.93867193946857	37.0	35.0	37.0	35.0	37.0
5	35.77971240801161	37.0	35.0	37.0	35.0	37.0
6	35.78988561489926	37.0	35.0	37.0	35.0	37.0
7	35.946254237072964	37.0	35.0	37.0	35.0	37.0
8	36.08458805706137	37.0	35.0	37.0	35.0	37.0
9	37.713189833563966	39.0	38.0	39.0	35.0	39.0
10	37.22903151408972	39.0	37.0	39.0	34.0	39.0
11	37.217234360387124	39.0	37.0	39.0	34.0	39.0
12	37.2074314345322	39.0	37.0	39.0	34.0	39.0
13	37.18601437959942	39.0	37.0	39.0	33.0	39.0
14	38.416237140668706	40.0	38.0	41.0	34.0	41.0
15	38.38703148869902	40.0	38.0	41.0	34.0	41.0
16	38.32755800716864	40.0	38.0	41.0	34.0	41.0
17	38.31936739072478	40.0	38.0	41.0	34.0	41.0
18	38.27575886452792	40.0	38.0	41.0	34.0	41.0
19	38.299927636506744	40.0	38.0	41.0	34.0	41.0
20	38.23003233082389	40.0	37.0	41.0	34.0	41.0
21	38.216480045026174	40.0	37.0	41.0	34.0	41.0
22	38.19563322288379	40.0	37.0	41.0	34.0	41.0
23	38.124292763227494	40.0	37.0	41.0	34.0	41.0
24	38.044873829382965	40.0	37.0	41.0	33.0	41.0
25	38.062120461941454	40.0	37.0	41.0	33.0	41.0
26	38.01189871650015	40.0	37.0	41.0	33.0	41.0
27	37.96523801664784	40.0	37.0	41.0	33.0	41.0
28	37.926452665388666	40.0	37.0	41.0	33.0	41.0
29	37.81966255760515	40.0	37.0	41.0	33.0	41.0
30	37.83532967707262	40.0	37.0	41.0	33.0	41.0
31	37.78059790865273	40.0	37.0	41.0	33.0	41.0
32	37.76231237331099	40.0	37.0	41.0	33.0	41.0
33	37.7359197992442	40.0	37.0	41.0	33.0	41.0
34	37.67158611467286	40.0	37.0	41.0	33.0	41.0
35	37.603816222117835	40.0	37.0	41.0	33.0	41.0
36	37.60265142378347	40.0	37.0	41.0	33.0	41.0
37	37.50571184941622	40.0	36.0	41.0	33.0	41.0
38	37.47882204081978	40.0	36.0	41.0	33.0	41.0
39	37.43660365541435	40.0	36.0	41.0	32.0	41.0
40	37.38730126488001	40.0	36.0	41.0	32.0	41.0
41	37.334058026211665	39.0	36.0	41.0	32.0	41.0
42	37.23840808778411	39.0	36.0	41.0	32.0	41.0
43	37.17694143635187	39.0	36.0	41.0	32.0	41.0
44	37.13341648787382	39.0	35.0	41.0	32.0	41.0
45	36.99250445395185	39.0	35.0	41.0	31.0	41.0
46	36.885219227530285	39.0	35.0	41.0	31.0	41.0
47	36.779996995433905	39.0	35.0	40.0	31.0	41.0
48	36.73873922482195	39.0	35.0	40.0	31.0	41.0
49	36.61452369163842	39.0	35.0	40.0	31.0	41.0
50	36.46678473341881	39.0	35.0	40.0	30.0	41.0
51	35.33773756173114	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	10.0
11	5.0
12	3.0
13	10.0
14	18.0
15	20.0
16	39.0
17	80.0
18	141.0
19	306.0
20	522.0
21	899.0
22	1345.0
23	1887.0
24	2487.0
25	3118.0
26	4085.0
27	5473.0
28	7202.0
29	9929.0
30	12784.0
31	17272.0
32	23644.0
33	32548.0
34	50012.0
35	63220.0
36	78703.0
37	119628.0
38	206961.0
39	302802.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.052093251575283	30.720207188106997	21.572572966522362	27.65512659379536
2	22.77820800907294	29.588099379197402	20.897603541156208	26.73608907057345
3	21.463392959159062	30.29501876795863	21.528245037176216	26.713343235706095
4	20.66020050188949	30.78230860702391	20.23067450392921	28.326816387157383
5	19.270377094203727	32.70459613976734	17.70810851984918	30.316918246179757
6	20.476752698819755	36.37249425535426	19.752906177133983	23.397846868692
7	88.08731861519125	3.19203409124571	4.609998857418527	4.110648436144507
8	89.55839226091483	3.125806683678435	3.735712441865878	3.58008861354086
9	81.32852602758275	8.151789832717608	6.874531858980055	3.645152280719573
10	30.03698578544013	43.963361220784826	12.37881230771835	13.620840686056699
11	21.774111642905204	25.739292530479418	33.96989932587693	18.516696500738448
12	24.446905931690555	22.856284409687397	33.62976974867439	19.067039909947653
13	25.157951309102145	23.170917492922342	31.413267486786257	20.25786371118926
14	21.19605005353206	26.688693098384732	31.645380797014056	20.46987605106916
15	20.44892872407504	29.019347712932753	30.415095617142107	20.1166279458501
16	22.669451180032755	28.615952976424737	28.841930200967386	19.872665642575125
17	22.051399239125374	27.081931555138016	29.727959814986438	21.13870939075017
18	22.20194492757303	26.44314387639807	29.08494035301536	22.269970843013535
19	21.546230115908543	29.73917404054895	29.080391186041886	19.63420465750062
20	23.449474624111854	28.435361627036016	28.068042842573433	20.0471209062787
21	23.384305162352366	27.976318941038564	28.350725962413303	20.28864993419577
22	21.816641064378118	26.02335097986941	29.052038238393273	23.107969717359197
23	21.53057251795334	27.567740270072406	28.163998527339434	22.73768868463482
24	19.824423313740176	28.215203104436178	29.264367962015513	22.696005619808133
25	21.32226298840068	28.48275759922474	27.17809882906558	23.016880583309003
26	22.35217323227835	29.181425010685253	25.93755157485813	22.528850182178267
27	19.638753824474094	27.706225376311323	28.676044298306863	23.978976500907716
28	21.225355152407673	28.073967339097024	29.83259065537627	20.868086853119035
29	21.623883338199885	27.962248261795036	28.091952417829347	22.32191598217573
30	22.45013901407914	25.709035280376796	30.291844930535277	21.54898077500878
31	22.978371355905665	28.07597743613181	26.156757946230965	22.78889326173156
32	21.583575602923315	27.462368867617126	28.445412112209965	22.508643417249594
33	22.3225507496604	26.048530090094662	29.350167367026792	22.27875179321814
34	19.936565569365275	26.93487708785605	29.28764276978676	23.840914572991913
35	20.531977469986078	27.387254715264465	30.558024095773717	21.52274371897574
36	20.971553953120306	27.431053671706724	30.01371097766888	21.583681397504094
37	21.542209921838964	28.595640416915284	27.235651081009028	22.626498580236724
38	21.773476875420535	27.640844325390272	28.58929274206858	21.99638605712061
39	21.24323443655922	25.707659950826677	28.386801914458733	24.662303698155366
40	21.327975895762716	26.163211415658445	28.76067996292958	23.748132725649263
41	19.7268807102625	26.538993766583303	30.4655596321734	23.2685658909808
42	21.311577735742066	27.974837816907666	27.970288649934194	22.743295797416074
43	20.582547279598153	26.71619968938711	29.16999919596119	23.531253835053555
44	21.864671804051508	25.211165983233673	28.903925825303524	24.02023638741129
45	23.28305974854744	25.390910975976166	29.076476786553084	22.249552488923307
46	20.459613976733657	25.677614289885614	30.450219417960533	23.412552315420196
47	20.45993136047599	26.706043409632386	28.898001328779934	23.93602390111169
48	20.877819954550645	25.818744260643996	30.46471327552717	22.838722509278185
49	21.74544131151426	25.170540864214768	29.15846758665634	23.92555023761463
50	20.599791796265027	25.050358220450512	28.618386251782642	25.73146373150182
51	20.821325648414984	23.892859712154106	30.77003643565362	24.515778203777288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1159.0
1	970.0
2	781.0
3	570.5
4	360.0
5	366.0
6	372.0
7	413.0
8	454.0
9	497.0
10	540.0
11	626.5
12	713.0
13	844.5
14	976.0
15	1019.5
16	1063.0
17	1288.0
18	1513.0
19	1733.0
20	1953.0
21	2416.0
22	2879.0
23	3489.5
24	4100.0
25	5827.5
26	9183.0
27	10811.0
28	13450.5
29	16090.0
30	18765.5
31	21441.0
32	24656.0
33	27871.0
34	28333.0
35	28795.0
36	34022.0
37	39249.0
38	41534.0
39	43819.0
40	48485.0
41	53151.0
42	58257.5
43	63364.0
44	70886.5
45	78409.0
46	85632.5
47	92856.0
48	91318.0
49	89780.0
50	81823.5
51	73867.0
52	67099.5
53	60332.0
54	54366.0
55	48400.0
56	45335.0
57	42270.0
58	38617.5
59	34965.0
60	31813.0
61	28661.0
62	26211.0
63	23761.0
64	19812.5
65	15864.0
66	13343.5
67	10823.0
68	8467.0
69	6111.0
70	5147.5
71	4184.0
72	3493.0
73	2802.0
74	2135.5
75	1108.0
76	747.0
77	599.5
78	452.0
79	325.5
80	199.0
81	140.0
82	81.0
83	106.5
84	132.0
85	85.0
86	38.0
87	24.0
88	10.0
89	7.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	945228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.088432993798722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11326261638375	19.8052371534192
2	10.58691487196188	5.735658682403811
3	3.730061228088033	3.0312454091948773
4	1.8095618411818608	1.960727787319595
5	1.0780285522198756	1.4601052101104972
6	0.7818985200379316	1.270824340079874
7	0.6153480543027229	1.1668170175790609
8	0.47505139923675443	1.0294718397468117
9	0.4186549194548534	1.0206635159859294
>10	6.2953403081304895	41.95739914201042
>50	1.0302115347163572	17.618041652103486
>100	0.06134083873384371	2.733909316532809
>500	0.003538894542337135	0.5947773258861262
>1k	7.864210094082523E-4	0.6151216076275751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	4735	0.5009373399856966	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1040	0.11002636400952996	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5465348043011845	0.0
2	0.0	0.0	0.0	0.7798118549175437	0.0
3	0.0	0.0	0.0	1.014464235084022	0.0
4	0.0	0.0	0.0	2.0929341915389728	0.0
5	0.0	0.0	0.0	2.283047053197747	0.0
6	0.0	0.0	0.0	3.4060565281604016	0.0
7	0.0	0.0	0.0	4.234110711912893	0.0
8	0.0	0.0	0.0	4.944627116420588	0.0
9	0.0	0.0	0.0	5.871810822362435	0.0
10	0.0	0.0	0.0	7.222490235160194	0.0
11	0.0	0.0	0.0	8.540267533335872	0.0
12	0.0	0.0	0.0	9.23216409162657	0.0
13	0.0	0.0	0.0	9.506595234165726	0.0
14	0.0	0.0	0.0	9.818371863719653	0.0
15	0.0	0.0	0.0	10.090369730900905	0.0
16	0.0	0.0	0.0	10.604425598903122	0.0
17	0.0	0.0	0.0	11.362972743084208	0.0
18	0.0	0.0	0.0	12.160346498410965	0.0
19	0.0	0.0	0.0	12.607434396780459	0.0
20	0.0	0.0	0.0	13.05811931089642	0.0
21	0.0	0.0	0.0	13.551122057323736	0.0
22	0.0	0.0	0.0	14.139022542709272	0.0
23	0.0	0.0	0.0	14.694232502634286	0.0
24	0.0	0.0	0.0	15.125874392210134	0.0
25	0.0	0.0	0.0	15.493404765834274	0.0
26	0.0	0.0	0.0	15.865061128108774	0.0
27	0.0	0.0	0.0	16.251740320853806	0.0
28	0.0	0.0	0.0	16.6302733308789	0.0
29	0.0	0.0	0.0	17.045517060434097	0.0
30	0.0	0.0	0.0	17.588772232731152	0.0
31	0.0	0.0	0.0	17.99597557414719	0.0
32	0.0	0.0	0.0	18.389108236319704	0.0
33	0.0	0.0	0.0	18.79324353489317	0.0
34	0.0	0.0	0.0	19.191983309846936	0.0
35	0.0	0.0	0.0	19.62214407529189	0.0
36	0.0	0.0	0.0	20.021095439407212	0.0
37	0.0	0.0	0.0	20.42226848971888	0.0
38	0.0	0.0	0.0	20.828413885327137	0.0
39	0.0	0.0	0.0	21.235088253839287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.032651E-4	45.0	21
CGAACCG	35	1.2116288E-7	45.0	1
ATTCGCG	45	3.8380676E-10	45.0	1
ATCCGGT	20	7.032651E-4	45.0	33
TCGGACG	20	7.032651E-4	45.0	1
GTCACGC	20	7.032651E-4	45.0	13
CGTAACG	50	2.1827873E-11	45.0	1
TAGTGCG	20	7.032651E-4	45.0	1
ACTCACG	35	1.2116288E-7	45.0	1
TCGACAG	40	6.8121153E-9	45.0	1
TAAGACG	25	3.8902785E-5	45.0	1
CCGTTGC	20	7.032651E-4	45.0	30
CCGCAGT	20	7.032651E-4	45.0	38
TCGAACG	35	1.2116288E-7	45.0	1
CCGCACG	20	7.032651E-4	45.0	1
CGATTCG	30	2.1650903E-6	44.999996	1
CGTTACG	30	2.1650903E-6	44.999996	1
TAGTACG	30	2.1650903E-6	44.999996	1
TGCGAAG	110	0.0	42.954544	1
ACGACCA	105	0.0	42.857147	28
>>END_MODULE