##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200292
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810973496449197	33.0	31.0	34.0	30.0	34.0
2	32.018091431085104	33.0	31.0	34.0	30.0	34.0
3	32.15657023457625	34.0	31.0	34.0	30.0	34.0
4	35.761727979524984	37.0	35.0	37.0	35.0	37.0
5	35.58611904436587	37.0	35.0	37.0	33.0	37.0
6	35.60057302723004	37.0	35.0	37.0	33.0	37.0
7	35.79334445284981	37.0	35.0	37.0	35.0	37.0
8	35.97498275419648	37.0	35.0	37.0	35.0	37.0
9	37.556809509685976	39.0	37.0	39.0	35.0	39.0
10	37.03565632362792	39.0	37.0	39.0	33.0	39.0
11	36.99048731475341	39.0	37.0	39.0	33.0	39.0
12	37.04301453313027	39.0	37.0	39.0	33.0	39.0
13	37.02642523652578	39.0	37.0	39.0	33.0	39.0
14	38.22131364701256	40.0	38.0	41.0	33.0	41.0
15	38.202531550656005	40.0	37.0	41.0	33.0	41.0
16	38.14204960126369	40.0	37.0	41.0	33.0	41.0
17	38.12866285870438	40.0	37.0	41.0	33.0	41.0
18	38.09016889223622	40.0	37.0	41.0	33.0	41.0
19	38.11331076104815	40.0	37.0	41.0	33.0	41.0
20	38.03180309458032	40.0	37.0	41.0	33.0	41.0
21	38.0320555331536	40.0	37.0	41.0	33.0	41.0
22	38.01787315086662	40.0	37.0	41.0	33.0	41.0
23	37.92555311540858	40.0	37.0	41.0	33.0	41.0
24	37.84702139146141	40.0	37.0	41.0	33.0	41.0
25	37.87084809363055	40.0	37.0	41.0	33.0	41.0
26	37.76658263155965	40.0	37.0	41.0	33.0	41.0
27	37.71335641660529	40.0	37.0	41.0	33.0	41.0
28	37.70360295661389	40.0	37.0	41.0	33.0	41.0
29	37.55008697883515	40.0	37.0	41.0	32.0	41.0
30	37.59528181475841	40.0	37.0	41.0	32.0	41.0
31	37.491222969077526	40.0	37.0	41.0	32.0	41.0
32	37.46342056766187	39.0	36.0	41.0	32.0	41.0
33	37.42752596868095	39.0	36.0	41.0	32.0	41.0
34	37.36674325914028	39.0	36.0	41.0	32.0	41.0
35	37.26774151623105	39.0	36.0	41.0	31.0	41.0
36	37.2591394427356	39.0	36.0	41.0	31.0	41.0
37	37.152403748421214	39.0	36.0	41.0	31.0	41.0
38	37.15372592669117	39.0	36.0	41.0	31.0	41.0
39	37.108326140639114	39.0	36.0	41.0	31.0	41.0
40	37.053736924015155	39.0	36.0	41.0	31.0	41.0
41	36.996383380044186	39.0	36.0	41.0	31.0	41.0
42	36.85852775824549	39.0	35.0	41.0	31.0	41.0
43	36.776937611847785	39.0	35.0	40.0	31.0	41.0
44	36.743524908938824	39.0	35.0	40.0	31.0	41.0
45	36.55506826672176	39.0	35.0	40.0	30.0	41.0
46	36.3891694687626	39.0	35.0	40.0	30.0	41.0
47	36.30027776574367	39.0	35.0	40.0	30.0	41.0
48	36.24027236705735	38.0	35.0	40.0	29.0	41.0
49	36.099578269287804	38.0	35.0	40.0	29.0	41.0
50	35.91328610038224	38.0	35.0	40.0	28.0	41.0
51	34.66883891586381	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	3.0
13	6.0
14	12.0
15	29.0
16	37.0
17	101.0
18	244.0
19	386.0
20	794.0
21	1213.0
22	1969.0
23	2605.0
24	3621.0
25	4812.0
26	6467.0
27	8487.0
28	11347.0
29	15364.0
30	20216.0
31	26992.0
32	35076.0
33	46160.0
34	66235.0
35	84488.0
36	110156.0
37	160215.0
38	260771.0
39	332387.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.16266375182039	32.80776677675099	21.593912148043977	27.435657323384643
2	22.134613910615084	29.64553625284514	22.0423030395937	26.17754679694608
3	19.43810339484059	31.14342176736994	22.494193079683946	26.92428175810553
4	20.667220976229117	31.751607108936824	19.746944910071882	27.834227004762173
5	18.128005518657126	33.965318439179796	18.197155358862677	29.709520683300394
6	21.552255617799666	34.93449927184385	19.736447464450315	23.776797645906164
7	87.08939158138186	3.783579329029936	4.855901730578893	4.2711273590093075
8	88.6251012253685	2.982857504673863	3.8891369766690103	4.5029042932886325
9	79.67136330159661	8.424949928850646	7.791937295258154	4.111749474294588
10	29.99378484568755	44.278725510125874	12.479963208952489	13.247526435234095
11	19.158004885477865	26.115728506063522	36.0398136453463	18.68645296311231
12	23.376894955560815	22.60741552888797	33.87692328200138	20.138766233549838
13	23.486285003982367	24.401395660389305	31.12234356306632	20.989975772562012
14	18.847747048218267	27.10457122100289	31.522496192593135	22.525185538185706
15	18.109426706168165	30.529737763810804	30.446258077201215	20.914577452819813
16	20.748451210205516	28.530057685963083	29.61387728985947	21.107613813971934
17	19.853252375255355	28.433081283554333	30.650874953761253	21.06279138742906
18	20.653391008188006	27.0459188264189	29.459081623471622	22.841608541921467
19	20.466519813512047	30.14599780720025	29.027769909322064	20.35971246996564
20	21.790780909978572	29.637621512098722	28.104327946866263	20.467269631056443
21	21.60191020185088	28.972533350218114	28.43633049291339	20.989225955017613
22	20.89608195339134	27.133230913810973	28.93987463050658	23.03081250229111
23	19.94781269890993	28.737507206579732	27.739333428865642	23.57534666564469
24	18.60880519073692	29.157321718381862	29.051764070742784	23.182109020138434
25	21.22258583744622	29.14640770745785	26.17596384879679	23.455042606299134
26	22.084792700442893	30.39352090991192	25.960516274373234	21.56117011527195
27	20.065700679501322	28.525725406817674	27.431158418118258	23.977415495562745
28	21.308231663628515	27.84247499775055	28.559633822436542	22.289659516184397
29	21.69830341283621	28.41858481102932	26.94394364038084	22.939168135753633
30	24.492123583261407	25.88511795463104	26.060408633899083	23.562349828208472
31	21.826688839049165	28.72867602216794	24.554858317809334	24.889776820973562
32	22.98090797905843	27.92203897051718	26.512798552352262	22.584254498072138
33	22.576506383446695	27.11356903153566	25.204783502681018	25.10514108233663
34	21.061791630703194	26.734494606312463	27.29894059112283	24.904773171861514
35	21.758122190267034	27.47481446181429	26.79656283637648	23.97050051154219
36	21.6076588030246	28.012350328086832	26.734661232433442	23.645329636455127
37	20.83792943717029	28.430748517860653	25.77547796702802	24.955844077941034
38	20.264902207129598	26.6522646156102	28.57304722517521	24.509785952084993
39	19.67787838292682	26.15746834936832	27.88504797166023	26.27960529604463
40	22.315569877996356	26.591529394514	26.65201467642874	24.440886051060907
41	19.79809912921189	26.0983160764214	29.26721164516634	24.836373149200362
42	22.391801328343437	28.507729785752133	25.653341020351718	23.447127865552716
43	20.86025733738124	26.941277622445202	28.416835236759052	23.781629803414503
44	23.068470005631962	25.57044452516554	26.258443778680522	25.10264169052197
45	22.983573996994064	25.355080263802478	27.991188810722722	23.670156928480736
46	19.93031695620732	26.47855688449144	29.256714199544774	24.33441195975646
47	21.138189707171254	26.514214874380563	26.64768239728333	25.69991302116485
48	21.108696883758284	25.481216237382238	29.422757129098585	23.987329749760892
49	22.186434634239003	24.663665174807463	28.130071682557244	25.01982850839629
50	20.139932616396678	24.122213594691956	27.91679024770639	27.82106354120497
51	20.110023227681264	23.138869541744842	30.51582448270921	26.235282747864687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1340.0
1	1093.0
2	846.0
3	624.5
4	403.0
5	418.0
6	433.0
7	524.5
8	616.0
9	705.5
10	795.0
11	951.0
12	1107.0
13	1263.5
14	1420.0
15	1542.5
16	1665.0
17	1885.5
18	2106.0
19	2244.5
20	2383.0
21	2995.5
22	3608.0
23	4793.5
24	5979.0
25	6740.5
26	9782.0
27	12062.0
28	15065.0
29	18068.0
30	21088.5
31	24109.0
32	28806.0
33	33503.0
34	37787.0
35	42071.0
36	45441.5
37	48812.0
38	51514.5
39	54217.0
40	57350.0
41	60483.0
42	63415.5
43	66348.0
44	70846.0
45	75344.0
46	85271.0
47	95198.0
48	105697.5
49	116197.0
50	123899.0
51	131601.0
52	121203.0
53	110805.0
54	96839.5
55	82874.0
56	69751.0
57	56628.0
58	48529.5
59	40431.0
60	36763.5
61	33096.0
62	29491.0
63	25886.0
64	21303.5
65	16721.0
66	13143.5
67	9566.0
68	7510.5
69	5455.0
70	4613.5
71	3772.0
72	3395.0
73	3018.0
74	2442.0
75	1493.0
76	1120.0
77	738.5
78	357.0
79	274.5
80	192.0
81	160.5
82	129.0
83	112.5
84	96.0
85	64.0
86	32.0
87	24.5
88	17.0
89	14.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1200292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.660906632777017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99754260657977	21.651732956759247
2	11.144190872462815	6.610936099319309
3	4.005946695514596	3.5646003273462012
4	2.004349021096694	2.378032366969882
5	1.2704218613850777	1.8840932107390789
6	0.8544650124132873	1.52065241724991
7	0.6673163713141257	1.3855246008850293
8	0.5405941880863745	1.2827610991241483
9	0.45122010860246975	1.2045237760880425
>10	5.099280604151879	34.245422816118975
>50	0.8396705265923602	16.864084545860564
>100	0.11934592674354702	5.270520948799995
>500	0.003676533287099569	0.750259256659385
>1k	0.001696861517122878	0.7820513248921563
>5k	2.8281025285381307E-4	0.6048042531880775
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	7210	0.6006871661229101	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	2542	0.2117817997620579	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	1654	0.13779980204816827	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1579	0.13155132251152218	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1425	0.11872111119627558	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6837502874300587	0.0
2	0.0	0.0	0.0	0.965015179639621	0.0
3	0.0	0.0	0.0	1.2996837436223851	0.0
4	0.0	0.0	0.0	2.7255867738850212	0.0
5	0.0	0.0	0.0	2.996187594352041	0.0
6	0.0	0.0	0.0	4.45983144101602	0.0
7	0.0	0.0	0.0	5.645292978708515	0.0
8	0.0	0.0	0.0	6.7829328196805445	0.0
9	0.0	0.0	0.0	8.188840715425913	0.0
10	0.0	0.0	0.0	10.126619189330595	0.0
11	0.0	0.0	0.0	11.934679228054506	0.0
12	0.0	0.0	0.0	12.827711923431965	0.0
13	0.0	0.0	0.0	13.25194202743999	0.0
14	0.0	0.0	0.0	13.674422557177753	0.0
15	0.0	0.0	0.0	14.019505253721594	0.0
16	0.0	0.0	0.0	14.653351017918974	0.0
17	0.0	0.0	0.0	15.54705021778034	0.0
18	0.0	0.0	0.0	16.523146034464947	0.0
19	0.0	0.0	0.0	17.08809189763824	0.0
20	8.331306048861444E-5	0.0	0.0	17.660619249316	0.0
21	8.331306048861444E-5	0.0	0.0	18.305379024437386	0.0
22	8.331306048861444E-5	0.0	0.0	19.047531767269966	0.0
23	8.331306048861444E-5	0.0	0.0	19.747778040676767	0.0
24	1.6662612097722887E-4	0.0	0.0	20.258487101471975	0.0
25	1.6662612097722887E-4	0.0	0.0	20.70562829711437	0.0
26	1.6662612097722887E-4	0.0	0.0	21.143188490800572	0.0
27	1.6662612097722887E-4	0.0	0.0	21.628237128965285	0.0
28	1.6662612097722887E-4	0.0	0.0	22.108037044319214	0.0
29	1.6662612097722887E-4	0.0	0.0	22.62791054176817	0.0
30	1.6662612097722887E-4	0.0	0.0	23.321908335638327	0.0
31	2.499391814658433E-4	0.0	0.0	23.8218700116305	0.0
32	2.499391814658433E-4	0.0	0.0	24.291255794423357	0.0
33	2.499391814658433E-4	0.0	0.0	24.77938701582615	0.0
34	2.499391814658433E-4	0.0	0.0	25.247439789651185	0.0
35	2.499391814658433E-4	0.0	0.0	25.76064824226105	0.0
36	2.499391814658433E-4	0.0	0.0	26.24752976775651	0.0
37	2.499391814658433E-4	0.0	0.0	26.737993754852987	0.0
38	2.499391814658433E-4	0.0	0.0	27.231040446824608	0.0
39	2.499391814658433E-4	0.0	0.0	27.727752913457724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.033411E-4	45.000004	1
AGATCGT	20	7.033411E-4	45.000004	34
GTCGAAA	20	7.033411E-4	45.000004	17
CTATACG	45	3.8562575E-10	45.000004	1
GAATCGC	20	7.033411E-4	45.000004	9
TCTACCG	30	2.1655815E-6	45.000004	1
CGCTAGT	20	7.033411E-4	45.000004	39
TTGCGAG	40	6.8157533E-9	45.000004	1
TCCCGAA	20	7.033411E-4	45.000004	15
TATAGCG	40	6.8157533E-9	45.000004	1
CCGAACG	30	2.1655815E-6	45.000004	1
CACGTAG	20	7.033411E-4	45.000004	1
TATCGCG	20	7.033411E-4	45.000004	1
CGATACG	45	3.8562575E-10	45.000004	1
GCGTATG	30	2.1655815E-6	45.000004	1
TCGCGCG	40	6.8157533E-9	45.000004	1
GCCCGAC	20	7.033411E-4	45.000004	31
CGAAACG	75	0.0	45.0	1
GGCGTAA	25	3.89091E-5	45.0	20
CATACCG	75	0.0	45.0	1
>>END_MODULE